SSGCID: Target Page

TrbrA.19527.a

Glucosyl transferase (HmdUGT)

CENTER ID:	TrbrA.19527.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19527.a.B2.GE41490	nucleoside diphospho-sugar transferase-like domain		9	429
TrbrA.19527.a.B3.GE41491	C-term domain		525	655

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19527.a.B3.PS38333	C-term domain		525	655

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.10.6900
UniProt:	Q4FKJ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGGPSEGKLY CDNGASAFDY PGAATCPQGV KGCAGLTENP YFYSLDTKFN NEGNNGIVTA DTRGTVYGHT CPIFVPSKGR FYHERGTLSL LIGDMVPFSL VVEKEEEAQY RELVDRLISR FWGEGADRVS HSGARTKLRK EEGATPRRGG SRTGNVPPSV PAVLQQQVLA GSELQMARNL VKIEVLPSSG RGVAYVRNYI LRKLAPAAVN VYANAGTPSC KPNSVSPGGR SHAAQWYWVM DDDIAKFFVA ADKKSTVITA REALKRVYSR IQRLHGTVCD MNEVAVFSLE YNQFAYAYGD SDVKINGYNT VASLYRHDLL PRCCEFRFPI REDYDFTLQV IAHGLKTARF RNLSFAVPTM GGLAGGMTEY YKNKKDDICQ QNKLFVRTWP SVAQECVKGK GVTLRNDIRV LWGLLGPKCN TVPSLTLQSR SKLPRASNRN ANSATRTSSL RATITSLSKA KKTSAATKVL VVQRSKAKTF RGGAYARRKA EGTDDGGGDC STQGVTSAVR EAAEVGNANG EESSAWKGYV MKKWRYLTNE EAKGLHLTLL PPEKLRCGDT VAVVMPYLEQ HPSVVRATLI EKTYKMRRME VAGEDGEVAS ENGKCKYRKV VEWEVAPDNF PLMMVSCCYE VPPEGVEVAA AAVDAFFGRG GSQAD

NT Sequence

atgggaggtc caagtgaggg gaagttatat tgtgacaacg gggcttccgc cttcgactat cctggagccg cgacgtgccc tcaaggcgta aagggttgcg caggcctgac tgagaaccct tacttctatt ctctcgatac gaagtttaac aatgaaggaa ataacggcat cgtaaccgcc gacacgcgcg gcactgtata tggtcacacg tgcccaattt ttgttccatc gaaggggagg ttttatcacg agcgagggac cttgtcgctc cttattgggg atatggtgcc attttccctt gtcgtggaga aagaggaaga ggctcaatat agggagcttg tcgaccggct gatttcccgt ttttggggtg agggcgctga ccgtgtttcc cacagcggtg cccggacgaa gttgaggaaa gaggagggtg ctacaccacg gaggggtgga tcacgcactg gtaatgtgcc tccctccgtt ccggcggtac tgcagcagca ggtgctcgcg ggtagtgagc tgcaaatggc gcggaacctg gtcaaaatcg aagtgctgcc gtcgtctggt aggggtgttg cgtacgttcg taactatatt ctgcgaaaac tcgctcctgc agcagtgaat gtgtatgcca acgccggaac cccctcgtgc aaacccaaca gtgtcagccc cgggggtcgt tcgcacgcag cccagtggta ttgggtgatg gatgatgata tagctaagtt ttttgttgca gccgacaaga aaagtaccgt gatcactgcg cgtgaggccc tcaagcgggt atattcacgg atccagcggc ttcatggaac ggtgtgcgat atgaacgagg tagctgtgtt ttccttggaa tataaccagt tcgcgtacgc ctacggtgat agcgatgtta aaatcaatgg ctacaataca gttgcatccc tttaccgcca tgatctgctt ccccggtgtt gcgagttccg tttccccatc cgtgaggatt acgacttcac ccttcaggtg attgcccacg gtctaaagac tgcgcggttt cggaatcttt catttgcggt acccactatg gggggactcg cggggggcat gactgagtac tacaagaaca aaaaggatga catctgccag cagaacaaat tgttcgtgag gacatggcct tctgtggctc aggagtgtgt gaaaggcaag ggcgtaacgc ttcgaaacga catccgtgta ctttggggtt tgctgggccc aaagtgtaac acagtcccca gtctgacact ccagtcgcgc tcgaagctgc cacgtgcaag caatagaaat gctaattctg ccaccagaac gtcgtccctg agggcgacca tcacgtccct atcgaaagct aagaaaactt ccgccgccac caaagttctc gtggtgcagc gttcaaaggc taaaacgttc cgcgggggtg cgtatgccag gaggaaggcg gaggggacgg acgatggtgg tggcgactgt tcaacgcaag gggtgacctc agcagtgcgc gaagctgcgg aggtggggaa tgctaatggc gaggaatcct ccgcgtggaa gggatatgta atgaagaagt ggagatacct cacgaatgaa gaggcgaaag ggttgcattt aactctcctt ccaccagaaa agttgaggtg tggggatacc gttgctgtgg tgatgcctta cctggagcag cacccttccg tggtaagggc gacgctaatt gaaaagacgt acaaaatgag gcgaatggag gtcgccgggg aagatggtga agttgcctca gagaacggaa aatgcaaata tcgaaaggtg gtggagtggg aagtggcgcc tgataatttt cctctcatga tggtaagctg ttgctacgag gtaccaccgg agggcgttga ggttgcggcc gcagccgttg atgccttttt tggtcgagga gggtcgcagg cagactaa

Details for TrbrA.19527.a.B2.GE41490

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML YCDNGASAFD YSGAATCPQG VKGCAGLTEN PYFYSLDTKF NNEGNNGIIT ADPRGTVYGH TCPIFVPSKG RFYHERGTLS LLIGDMVPFS LVVEKEEEAQ YRELVDRLIS RFWGEGADRV SHSGARTKLR KEEGATLRRG GSRTGNVPPS VPAVLRQQVL AGSELQMARN LVKIEVLPSS GRGVAYVRNY ILRKLAPAAV NVYANAGTPS CKPNSVSPGG RSHAAQWYWV MDDDIAKFFV AADKKSTVIT AREALKRVYS RIQRLHGTVC DMNEVAVFSL EYNQFAYAYG DSDVKINGYN TVASLYRHDL LPRCCEFRFP IREDYDFTLQ VIAHGLKTAR FRNLSFAVPT MGGLAGGMTE YYKNKKDDIC QQNKLFVRTW PSVAQECVKG KGVTLRNDIR VLWGLLGPKC NT

Validated NT Sequence

atggctcacc accaccacca ccatatgtta tattgtgaca acggggcttc cgccttcgac tattctggag ccgcgacgtg ccctcaaggc gtaaagggtt gcgcaggcct gactgagaac ccttacttct attctctcga tacgaagttt aacaatgaag gaaataacgg catcataacg gccgacccgc gcggcactgt atatggtcac acgtgcccaa tttttgttcc atcgaagggg aggttttatc acgagcgagg gaccttgtcg ctccttattg gggatatggt gccattttcc cttgtcgtgg agaaagagga agaggctcaa tatagggagc ttgtcgaccg gctgatttcc cgtttttggg gtgagggcgc tgaccgtgtt tcccacagcg gtgcccgaac gaagttgagg aaagaggagg gtgctacact acggaggggt ggatcacgca ctggtaatgt gcctccctcc gttccggcgg tactgcggca gcaggtgctc gcgggtagtg agctgcaaat ggcgcggaac ctggtcaaaa tcgaagtgct gccgtcgtct ggtaggggtg ttgcgtacgt tcgtaactat attctgcgaa aactcgctcc tgcagcagtg aatgtgtatg ccaacgccgg aactccctcg tgtaaaccca acagtgtcag ccccgggggt cgttcgcacg cagcccaatg gtattgggtg atggatgatg atatagctaa gttttttgtc gcagccgaca agaaaagtac cgtgatcact gcgcgtgagg ccctcaagcg ggtatattca cggatccagc ggcttcatgg aacggtgtgc gatatgaacg aggtagctgt gttttccttg gaatataacc agttcgcgta cgcctacggt gatagcgatg ttaaaatcaa tggctacaat acagttgcat ccctttaccg ccatgatcta cttccccggt gttgcgagtt ccgtttcccc atccgtgagg attacgactt cacccttcag gtgattgccc atggtctaaa gactgcgcgg tttcggaatc tttcatttgc ggtgcccact atggggggac tcgcgggggg catgactgag tactacaaga acaaaaagga tgacatctgc cagcagaaca aattgttcgt gaggacatgg ccttctgtgg ctcaggagtg tgtgaaaggc aagggcgtaa cgcttcgaaa cgacatccgt gtactttggg gtttgctggg cccaaagtgc aacacag

Expected Protein Sequence

MAHHHHHHLY CDNGASAFDY PGAATCPQGV KGCAGLTENP YFYSLDTKFN NEGNNGIVTA DTRGTVYGHT CPIFVPSKGR FYHERGTLSL LIGDMVPFSL VVEKEEEAQY RELVDRLISR FWGEGADRVS HSGARTKLRK EEGATPRRGG SRTGNVPPSV PAVLQQQVLA GSELQMARNL VKIEVLPSSG RGVAYVRNYI LRKLAPAAVN VYANAGTPSC KPNSVSPGGR SHAAQWYWVM DDDIAKFFVA ADKKSTVITA REALKRVYSR IQRLHGTVCD MNEVAVFSLE YNQFAYAYGD SDVKINGYNT VASLYRHDLL PRCCEFRFPI REDYDFTLQV IAHGLKTARF RNLSFAVPTM GGLAGGMTEY YKNKKDDICQ QNKLFVRTWP SVAQECVKGK GVTLRNDIRV LWGLLGPKCN TVPSLTLQS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttatatt gtgacaacgg ggcttccgcc ttcgactatc ctggagccgc gacgtgccct caaggcgtaa agggttgcgc aggcctgact gagaaccctt acttctattc tctcgatacg aagtttaaca atgaaggaaa taacggcatc gtaaccgccg acacgcgcgg cactgtatat ggtcacacgt gcccaatttt tgttccatcg aaggggaggt tttatcacga gcgagggacc ttgtcgctcc ttattgggga tatggtgcca ttttcccttg tcgtggagaa agaggaagag gctcaatata gggagcttgt cgaccggctg atttcccgtt tttggggtga gggcgctgac cgtgtttccc acagcggtgc ccggacgaag ttgaggaaag aggagggtgc tacaccacgg aggggtggat cacgcactgg taatgtgcct ccctccgttc cggcggtact gcagcagcag gtgctcgcgg gtagtgagct gcaaatggcg cggaacctgg tcaaaatcga agtgctgccg tcgtctggta ggggtgttgc gtacgttcgt aactatattc tgcgaaaact cgctcctgca gcagtgaatg tgtatgccaa cgccggaacc ccctcgtgca aacccaacag tgtcagcccc gggggtcgtt cgcacgcagc ccagtggtat tgggtgatgg atgatgatat agctaagttt tttgttgcag ccgacaagaa aagtaccgtg atcactgcgc gtgaggccct caagcgggta tattcacgga tccagcggct tcatggaacg gtgtgcgata tgaacgaggt agctgtgttt tccttggaat ataaccagtt cgcgtacgcc tacggtgata gcgatgttaa aatcaatggc tacaatacag ttgcatccct ttaccgccat gatctgcttc cccggtgttg cgagttccgt ttccccatcc gtgaggatta cgacttcacc cttcaggtga ttgcccacgg tctaaagact gcgcggtttc ggaatctttc atttgcggta cccactatgg ggggactcgc ggggggcatg actgagtact acaagaacaa aaaggatgac atctgccagc agaacaaatt gttcgtgagg acatggcctt ctgtggctca ggagtgtgtg aaaggcaagg gcgtaacgct tcgaaacgac atccgtgtac tttggggttt gctgggccca aagtgtaaca cagtccccag tctgacactc cagtcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for TrbrA.19527.a.B3.GE41491

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	16kDa
OBSERVED MW:	16kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA WKGYVMKKWR YLTNEEAEGL HLTLLPPEKL RCGDTVAVVM PYLEQHPSVV RATLIEKTYK MKRMEVAGED GEVASENGKC KYRKVVEWEV APDNFPLMMV SCCYEVPPEG VEVAAAAVDA FFGRGGSQAD

Validated NT Sequence

atggctcacc accaccacca ccatatggcg tggaagggat atgtaatgaa gaagtggaga tacctcacga atgaagaggc ggaagggttg catttaactc tcctcccacc agaaaagttg aggtgtgggg ataccgttgc tgtggtgatg ccttacctgg agcagcaccc ttccgtggta agggcgacgc taattgaaaa gacgtacaaa atgaagcgaa tggaggtcgc cggggaagat ggtgaagttg cctcagagaa cggaaaatgc aaatatcgaa aggtggtgga gtgggaagtg gcgcctgata attttcctct catgatggta agctgttgct acgaggtacc accggagggc gttgaggttg cggccgcagc cgttgatgcc ttttttggtc gaggagggtc gcaggcagac taagtgagta agat

Expected Protein Sequence

MAHHHHHHAW KGYVMKKWRY LTNEEAKGLH LTLLPPEKLR CGDTVAVVMP YLEQHPSVVR ATLIEKTYKM RRMEVAGEDG EVASENGKCK YRKVVEWEVA PDNFPLMMVS CCYEVPPEGV EVAAAAVDAF FGRGGSQAD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgtgga agggatatgt aatgaagaag tggagatacc tcacgaatga agaggcgaaa gggttgcatt taactctcct tccaccagaa aagttgaggt gtggggatac cgttgctgtg gtgatgcctt acctggagca gcacccttcc gtggtaaggg cgacgctaat tgaaaagacg tacaaaatga ggcgaatgga ggtcgccggg gaagatggtg aagttgcctc agagaacgga aaatgcaaat atcgaaaggt ggtggagtgg gaagtggcgc ctgataattt tcctctcatg atggtaagct gttgctacga ggtaccaccg gagggcgttg aggttgcggc cgcagccgtt gatgcctttt ttggtcgagg agggtcgcag gcagactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for TrbrA.19527.a.B3.PS38333

PURIFICATION DATe:	8/8/2017
CONCENTRATION:	6.49mg/ml
OBSERVED MW:	16kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMA WKGYVMKKWR YLTNEEAEGL HLTLLPPEKL RCGDTVAVVM PYLEQHPSVV RATLIEKTYK MKRMEVAGED GEVASENGKC KYRKVVEWEV APDNFPLMMV SCCYEVPPEG VEVAAAAVDA FFGRGGSQAD

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHAW KGYVMKKWRY LTNEEAKGLH LTLLPPEKLR CGDTVAVVMP YLEQHPSVVR ATLIEKTYKM RRMEVAGEDG EVASENGKCK YRKVVEWEVA PDNFPLMMVS CCYEVPPEGV EVAAAAVDAF FGRGGSQAD

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCGTGGA AGGGATATGT AATGAAGAAG TGGAGATACC TCACGAATGA AGAGGCGAAA GGGTTGCATT TAACTCTCCT TCCACCAGAA AAGTTGAGGT GTGGGGATAC CGTTGCTGTG GTGATGCCTT ACCTGGAGCA GCACCCTTCC GTGGTAAGGG CGACGCTAAT TGAAAAGACG TACAAAATGA GGCGAATGGA GGTCGCCGGG GAAGATGGTG AAGTTGCCTC AGAGAACGGA AAATGCAAAT ATCGAAAGGT GGTGGAGTGG GAAGTGGCGC CTGATAATTT TCCTCTCATG ATGGTAAGCT GTTGCTACGA GGTACCACCG GAGGGCGTTG AGGTTGCGGC CGCAGCCGTT GATGCCTTTT TTGGTCGAGG AGGGTCGCAG GCAGACTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T