TrbrA.19520.a

KREPA4

CENTER ID: TrbrA.19520.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.19520.a.B1.GE40794 full length 1 218
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: Tb927.10.5110
RefSeq: XP_822757.1
UniProt: Q38B91

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVRSLLLCI RRDPLQRAVD VAYASGMLLG SGSSRGTTHF SETTAFTSSN FAADLRGGRV GLAGDGGTKC DDRNGELVRG RIQFASRCAE GPFVGASPTV TSCAHIEGVV RQVECGYVGG DRVLQFIIEV EEVPASGGTP MRLPLAVRWR PGSEVAQNRM DEWQKEMERL VGRRVLASGR LQVEECFDSG SRRLYKTPSL VLPATSTVEM ISLQELEC
NT Sequence
atgcgggtgc gttcactcct tttgtgcatt cgtagggacc ccttgcagcg ggctgtcgat gtggcatatg cttccggtat gcttttaggt tcaggcagta gcagaggcac aacacatttc tcagaaacta ctgccttcac atcatcaaac tttgcggctg acctgagagg cggacgggtg ggacttgcag gtgatggggg gacaaaatgt gacgacagga acggagagct cgtgcgtggg cgaatccaat ttgcctcacg atgcgcggag ggaccatttg ttggtgcatc gccaactgtg acgtcatgcg cccacattga aggtgttgtg cgacaggtgg agtgtggtta tgtaggaggg gatcgggtgc ttcaatttat tatcgaggtt gaagaggtac ccgccagtgg aggcactcca atgcgtcttc ccttagcggt ccgttggcgg ccaggaagtg aagttgctca gaatcgtatg gatgagtggc aaaaggaaat ggagcgactg gtcgggcggc gggtgctggc cagtggccgg ttgcaggtgg aggaatgctt tgattcggga agtaggcgtt tgtacaagac tccttctctg gtgctccccg ctacgtcgac tgttgagatg atttctttgc aggagttgga gtgttaa
Details for TrbrA.19520.a.B1.GE40794
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR VRSLLLCIRR DPLQRAVDVA YASGMLLGSG SSRGTTHFSE TTAFTSSNFA ADLRGGRVGL AGDGGTKCED RNGELVRGRI QFASRCAEGP FVGASPTVTS CAHIEGVVRQ VECGYVGGDR VLQFIIEVEE VPASGGTPMR LPLAVRWRPG SEVAQNRMDE WQKEMERLVG RRVLASGRLQ VEECFDSGSR RLYKTPSLVL PATSTVEMIS LQELEC
Validated NT Sequence
atcttactca cttaacactc caactcctgc aaagaaatca tctcaacagt cgacgtagcg gggagcacca gagaaggagt cttgtacaaa cgcctacttc ccgaatcaaa gcattcctcc acctgcaacc ggccactggc cagcacccgc cgcccgacca gtcgctccat ttccttttgc cactcatcca tacgattctg agcaacttca cttcctggcc gccaacggac cgctaaggga agacgcattg gagtgcctcc actggcgggt acctcttcaa cctcgataat aaattgaagc acccgatccc ctcctacata accacactcc acctgtcgca caacaccttc aatgtgggcg catgacgtca cagttggcga tgcaccaaca aatggtccct ccgcgcatcg tgaggcaaat tggattcgcc cacgcacgag ttctccgttc ctgtcctcac attttgtccc cccatcacct gcaagtccca cccgtccgcc tctcaggtca gccgcaaagt ttgatgatgt gaaggcagta gtttctgaga aatgtgttgt gcctctgcta ctgcctgaac ctaaaagcat accggaagca tatgccacat cgacagcccg ctgcaagggg tccctacgaa tgcacaaaag gagtgaacgc acccgcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMR VRSLLLCIRR DPLQRAVDVA YASGMLLGSG SSRGTTHFSE TTAFTSSNFA ADLRGGRVGL AGDGGTKCDD RNGELVRGRI QFASRCAEGP FVGASPTVTS CAHIEGVVRQ VECGYVGGDR VLQFIIEVEE VPASGGTPMR LPLAVRWRPG SEVAQNRMDE WQKEMERLVG RRVLASGRLQ VEECFDSGSR RLYKTPSLVL PATSTVEMIS LQELEC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcggg tgcgttcact ccttttgtgc attcgtaggg accccttgca gcgggctgtc gatgtggcat atgcttccgg tatgctttta ggttcaggca gtagcagagg cacaacacat ttctcagaaa ctactgcctt cacatcatca aactttgcgg ctgacctgag aggcggacgg gtgggacttg caggtgatgg ggggacaaaa tgtgacgaca ggaacggaga gctcgtgcgt gggcgaatcc aatttgcctc acgatgcgcg gagggaccat ttgttggtgc atcgccaact gtgacgtcat gcgcccacat tgaaggtgtt gtgcgacagg tggagtgtgg ttatgtagga ggggatcggg tgcttcaatt tattatcgag gttgaagagg tacccgccag tggaggcact ccaatgcgtc ttcccttagc ggtccgttgg cggccaggaa gtgaagttgc tcagaatcgt atggatgagt ggcaaaagga aatggagcga ctggtcgggc ggcgggtgct ggccagtggc cggttgcagg tggaggaatg ctttgattcg ggaagtaggc gtttgtacaa gactccttct ctggtgctcc ccgctacgtc gactgttgag atgatttctt tgcaggagtt ggagtgttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at