SSGCID: Target Page

TrbrA.19519.a

KREPB10

CENTER ID:	TrbrA.19519.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19519.a.B1.GE40793	full length		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	Tb927.8.5700
RefSeq:	XP_847400.1
UniProt:	Q57X12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMYRWSPPSL SKKVKGDTFL SSLAQRVDSV AATSSSRQEP GGTLSNLRLG LRGSSTMCRS AFSLHSNQSL VGNRIFECPN KHIFLDTNAS YCHLCREPLG VSSFTHLSDR EHHNVQLFLF LSAAFPRGTL RDATDLSTTF AKTTWRAIGR GDNTAGESIL YHPDEVMDDA ARFCPRLLAA VKGGYAHTEQ EALRRASLQS MLILLSSGSI TSDGATRREP AVLGVFHGDV LPELAHSGER LFKGEVSRLI VEWFPALSAG VGTQIAHKCW GRSNLESVFD ALQLQTLINQ RCNRAAVRGS GSGWKSTLMK GLTTKTEKAT FMRLLMWELT TRNEDVGGDT NDGVSLNKVD RLLLELTRKR LAFEMVYLQS LKYMHLADAL LQEMSYPSLE DLVLIGAA

NT Sequence

atgatgtacc ggtggagccc cccgtcactc tccaaaaagg tgaagggaga tacatttctc tcttcgctgg cacaaagggt cgacagtgtt gctgctacat catcttctcg gcaggaaccg ggagggacgc tgagtaactt gcgtttgggg ttgcgcggaa gcagtacgat gtgccggagc gccttttcac ttcactccaa ccagagtttg gtgggaaatc gtatatttga atgtccaaac aaacatattt tcttggatac taatgcttcc tattgccatt tgtgccgcga gccgctcgga gtttcttcat tcacacatct ctctgaccgg gagcatcaca atgtccagtt atttctcttt ctatccgcag cattcccccg cggtacactt cgtgacgcaa ccgatctatc aacgactttt gcaaaaacca cctggagggc gattggcaga ggcgacaaca cggcagggga atcaatcctg tatcatccgg atgaggtgat ggatgacgct gcccggttct gtcctcgtct tttggccgcc gtgaagggtg gttatgcaca tacagaacag gaggccctac gccgcgccag tctgcaatcc atgcttattc tactcagtag cgggtcgatc acctcggacg gtgccactcg acgggaaccg gcggtactag gagtatttca tggtgatgtt ttacctgagt tggcgcacag cggtgaacga cttttcaagg gggaggtgtc acgtctcatt gtggaatggt tccccgcgtt aagtgcagga gtgggcacac agatagcaca caagtgctgg ggccgctcta atctggagag tgtttttgat gcccttcagt tacaaaccct aataaatcag cggtgcaaca gggcagcggt gcgggggtcg gggtcggggt ggaaaagcac cttgatgaag ggacttacaa ctaaaacgga gaaggcaaca tttatgcgac ttctgatgtg ggagctaacc acacgaaatg aagatgtagg tggagatacc aacgatggcg tgtctttaaa taaagtggac cgcttgcttc tggagctgac gaggaagcga cttgcattcg agatggttta tctccaatca ctgaagtaca tgcatttggc ggatgccctt ctacaggaga tgtcttaccc atccttggaa gatttggtat taataggcgc agcgtga

Details for TrbrA.19519.a.B1.GE40793

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	45kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMM YRWSPPSLSK KVKGDTFLSS LAQRAASVAA TSSSRQEPGG TLSNLRLGLR GSSTMCRSAF SLHSNQSLVG NRIFECPNKH IFLDTNASYC HLCREPLGVS SFTHLSDREH HNVQLFLFLS AAFPRGTLRD ATDLSTTFAK TTWRAIGRGD NTAGESILYH PDEVMDDAAR FCPRLLAAVK GGYAHTEQEA LRRASLQSML ILLSSGSITS DGATRREPAV LGVFHGDVLP ELAHSGERLF KGEVSRLIVE WFPALSAGVG TQITHKCWGR SNLESVFDAL QLQTLINQRC SMAAVRGSGS GWKSTLMKGL TTKTEKATFM RLLMWELTTR NEDVGGATND GVYLNKVDRL LLELTRKRLA FEMVYLQSLK YMQLADALLQ EMSYPSLE

Validated NT Sequence

tcttccaagg atgggtaaga catctcctgt agaagggcat ccgccaactg catgtacttc agtgattgga gataaaccat ctcgaatgca agtcgcttcc tcgtcagctc cagaagcaag cggtccactt tatttaaata cacgccatcg ttggtagctc cacctacatc ttcatttcgt gtggttagct cccacatcag aagtcgcata aatgttgcct tctccgtttt agttgtaagt cctttcatca aggtgctttt ccaccccgac cccgaccccc gcaccgctgc catgctgcac cgctgattta ttagggtttg taactgaagg gcatcaaaaa cactctccag attagagcgg ccccagcact tgtgtgttat ctgtgtgccc actcctgcac ttaacgcggg gaaccattcc acaatgagac gtgacacctc ccccttgaaa agtcgttcgc cgctgtgcgc caactcaggt aaaacatcac catgaaatac tcctagtacc gccggttccc gtcgagtggc accgtccgag gtgatcgacc cgctactgag tagaataagc atggattgca gactggcgcg gcgtagggcc tcctgttctg tatgtgcata accacccttc acggcggcca aaagacgagg acagaaccgg gcagcgtcat ccatcacctc atccggatga tacaggattg attcccctgc cgtgttgtcg cctctgccaa tcgccctcca ggtggtcttt gcaaaagtcg ttgatagatc ggttgcgtca cgaagtgtac ctcgggggaa tgctgcggat agaaagagaa atagctggac attgtgatgc tcccggtcag agagatgtgt gaatgaagaa actccgagcg gctcgcggca caaatggcaa taggaagcat tagtatccaa gaaaatatgt ttgtttggac attcaaatat acgatttccc accaaactct ggttggagtg aagtgaaaag gcgctccggc acatcgtact gcttccgcgc aaccccaaac gcaagttact cagcgtccct cccggttcct gccgagaaga tgatgtagca gcaacactgg cggccctttg tgccagcgaa gagagaaatg tatctccctt cacctttttg gagagtgacg gggggctcca ccggtacatc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMM YRWSPPSLSK KVKGDTFLSS LAQRVDSVAA TSSSRQEPGG TLSNLRLGLR GSSTMCRSAF SLHSNQSLVG NRIFECPNKH IFLDTNASYC HLCREPLGVS SFTHLSDREH HNVQLFLFLS AAFPRGTLRD ATDLSTTFAK TTWRAIGRGD NTAGESILYH PDEVMDDAAR FCPRLLAAVK GGYAHTEQEA LRRASLQSML ILLSSGSITS DGATRREPAV LGVFHGDVLP ELAHSGERLF KGEVSRLIVE WFPALSAGVG TQIAHKCWGR SNLESVFDAL QLQTLINQRC NRAAVRGSGS GWKSTLMKGL TTKTEKATFM RLLMWELTTR NEDVGGDTND GVSLNKVDRL LLELTRKRLA FEMVYLQSLK YMHLADALLQ EMSYPSLEDL VLIGAA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgt accggtggag ccccccgtca ctctccaaaa aggtgaaggg agatacattt ctctcttcgc tggcacaaag ggtcgacagt gttgctgcta catcatcttc tcggcaggaa ccgggaggga cgctgagtaa cttgcgtttg gggttgcgcg gaagcagtac gatgtgccgg agcgcctttt cacttcactc caaccagagt ttggtgggaa atcgtatatt tgaatgtcca aacaaacata ttttcttgga tactaatgct tcctattgcc atttgtgccg cgagccgctc ggagtttctt cattcacaca tctctctgac cgggagcatc acaatgtcca gttatttctc tttctatccg cagcattccc ccgcggtaca cttcgtgacg caaccgatct atcaacgact tttgcaaaaa ccacctggag ggcgattggc agaggcgaca acacggcagg ggaatcaatc ctgtatcatc cggatgaggt gatggatgac gctgcccggt tctgtcctcg tcttttggcc gccgtgaagg gtggttatgc acatacagaa caggaggccc tacgccgcgc cagtctgcaa tccatgctta ttctactcag tagcgggtcg atcacctcgg acggtgccac tcgacgggaa ccggcggtac taggagtatt tcatggtgat gttttacctg agttggcgca cagcggtgaa cgacttttca agggggaggt gtcacgtctc attgtggaat ggttccccgc gttaagtgca ggagtgggca cacagatagc acacaagtgc tggggccgct ctaatctgga gagtgttttt gatgcccttc agttacaaac cctaataaat cagcggtgca acagggcagc ggtgcggggg tcggggtcgg ggtggaaaag caccttgatg aagggactta caactaaaac ggagaaggca acatttatgc gacttctgat gtgggagcta accacacgaa atgaagatgt aggtggagat accaacgatg gcgtgtcttt aaataaagtg gaccgcttgc ttctggagct gacgaggaag cgacttgcat tcgagatggt ttatctccaa tcactgaagt acatgcattt ggcggatgcc cttctacagg agatgtctta cccatccttg gaagatttgg tattaatagg cgcagcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at