TrbrA.19513.a

KREN1

CENTER ID: TrbrA.19513.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.19513.a.B1.GE40789 full length 1 817
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: Tb927.1.1690
RefSeq: XP_001218871.1
UniProt: Q4GZ50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGLHWPLARS SNWCHMRALS NEHLRRRVGA AVRSIASTAP CFALERVEVA HSHAYSRRGV TVGFTSSSSP SGKTGSGGLS PASQSLHSSS SFDASNGDVL ANYVNFVADD ATGPAATKEN GAQPMRSLRS ASAVENLLFT DAAKNYCRLC MEEVQVTPKA HISTPYRGSH TNHTCREVVL DSLALLAIRG YPIDDVYFVW ADTLYQSSVF QRIPELVSPR WTVDKRSEVL AKILFMLKDM GVIDISLAAQ APDLFDNTAQ QVHHRRRVAF ERLEYIGDNS WGNHLSNRMM LLFPDRQWTY SQNAYTFNCF RDACEMNVTL EFMFDTLRVG ELLPPGVREK LGTGKIKADV VEAVIGELHV TLWGLEPQLY DSVCFVEING VGEARLAALV QHCLTEIYDL IVLSYVQELS GSAVPLAKQI AADRIWNSVY PPVRKAKDRA PGGRKSRSTV VNVVGVGEVR QLPSLPSLFP VASKRPTRAP HPLRRLRKLG EIPEETVCAG TNKDVFVHLI ESYERLDMLD DSLLPTLNMR RLQDVQFSKL KRQLVPSLSP AALEELHNCK ELDAESVTPH KVGDKEVATD ERVLDLEEVY FRDKYFDLFP TPLPNEVVTK EGDCDKRQSG ASTSRAVIIS LPRGFDGGRP CNRAQLHMAR NTIYPSLTER PAHSPTAGIT KGDRVCLFSL SVYVPPDTKR PSAGVVTDKN LHFGEFAFHG VRIKGIECAS EPECISRRSN APGSDVGTSL NRSSWKEGGD NSCGTLTVED GTGENSVVGE DGDTGVNGGE GKAEDATGKH LLRAKWCREN PFFPRASLPL LGISVGA
NT Sequence
atggggctac attggcccct ggcgcgtagt tccaactggt gccacatgcg ggcgttgagt aacgaacatt tgcggcgccg ggttggcgcc gccgtgcgca gcattgcctc aacggcaccc tgttttgctt tagaacgggt tgaggtggcg cattcgcatg cttattcccg ccgaggcgtg acagtgggct ttacgtcatc ttcgtcccct agcggcaaaa ctggcagtgg tgggctctcg ccagcatcac agtcgttgca ttcatcgtca tcttttgatg cgagtaatgg ggatgtgcta gccaattacg tcaacttcgt tgcagatgac gctaccggac ctgctgcgac caaagagaac ggtgcgcagc caatgcgtag tttgaggtcc gcctctgctg tcgaaaatct tctgtttacc gacgcagcga agaactattg ccgtctctgc atggaggagg tgcaggtgac gcccaaggcg catattagca ctccttaccg tgggagtcac acaaatcaca cctgtcggga ggtcgtactt gactctctcg ctttgcttgc cattcggggg taccctatcg atgatgttta cttcgtgtgg gcggacacgc tctatcaatc atctgtcttt caacgcatcc cagagctcgt ctccccgcgt tggactgttg ataagcggag tgaagtattg gctaagattc tatttatgtt aaaagacatg ggcgttatcg atatttcatt agccgcccag gcacccgatt tgtttgataa cacggcacaa caagtgcatc accgccggcg tgtggcgttc gaaagattag aatacatcgg tgataactcg tggggaaacc atctttccaa tcgtatgatg ctcctatttc ccgatcgcca gtggacttac agccaaaacg cctacacctt caactgcttt cgtgatgcgt gcgagatgaa tgtgacgttg gaatttatgt tcgatactct acgtgtgggg gagttacttc cacctggtgt acgggaaaaa ttgggaacgg ggaagataaa agcagatgtg gtggaagctg ttattggtga gttgcacgtg acattgtggg ggcttgagcc gcagctgtat gattctgtgt gctttgttga aattaacggc gtgggagagg cccgtctggc agcgttggtg cagcactgtc tcacagaaat atatgacctt atcgtgcttt cttacgttca ggaactcagt ggatctgcgg ttccgttagc aaagcagata gctgccgatc gcatatggaa cagcgtgtat cctcccgtac gaaaggcgaa ggaccgtgca cctgggggac gaaaaagtcg atctactgtt gtgaacgtag tcggtgtagg ggaggtacgg caacttccgt cacttccttc gctctttcct gtagcgtcaa agcgacccac acgggctcca catccacttc gccgacttag gaagttaggg gagattcctg aagaaactgt gtgtgctggt acgaacaagg atgtttttgt ccatctgatc gagagttatg aacgtctaga tatgttggat gactctctcc ttcccacatt gaatatgcga cggctccaag acgttcaatt ctcaaagctg aagcgacagt tggttccctc actctcccct gccgcgttgg aagaattgca caactgtaaa gagttggacg ctgaatcggt tacaccgcac aaggttggcg acaaggaggt tgcgacggat gaacgggtgc tggacttaga agaggtttat ttccgtgata agtattttga cctcttccca actccgttgc cgaatgaagt tgtcacaaaa gagggagact gtgacaaaag acaaagcggt gcctccacga gcagggcggt catcatatcg ctgccacgag ggtttgacgg gggtcgaccc tgcaacaggg cgcaactcca catggctcga aacacaattt atccttcact gactgaaaga ccggcccata gtcccactgc tggtattacg aagggtgatc gtgtgtgtct cttttctctt tcagtctatg tgcctccaga tacgaaaagg ccttctgcgg gcgtagtgac agacaagaac cttcactttg gtgaatttgc atttcatggc gtgcggatca agggaattga atgtgcctct gaaccagaat gtattagcag gcgaagcaat gctcctggtt ccgatgttgg gacctcactt aaccgcagtt catggaaaga aggaggtgac aattcatgtg gaacactaac tgttgaggat ggtaccggag agaactcggt agttggggaa gatggcgata ctggtgtgaa tggcggggaa ggtaaggctg aagatgcaac aggcaaacac ctactgcgag ccaaatggtg tcgtgaaaat cccttctttc cacgggcgtc gctacctctt ttaggcatct cggttggtgc gtga
Details for TrbrA.19513.a.B1.GE40789
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 91kDa
OBSERVED MW: 91kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG LHWPLARSSN WCHMRALSNE HLRRRVGAAV RSIASTAPCF ALERVEVAHS HAYSRRGVTV GFTSSSSPSG KTGSGGLSPA SQSLHSSSSF DASNGDVLAN YVNFVADDAT GPAATKENGA QPMRSLRSAS AVENLLFTDA AKNYCRLCME EVQVTPKAHI STPYRGSHTN HTCREVVLDS LALLAIRGYP IDDVYFVWAD TLYQSSVFQR IPELVSPRWT VDKRSEVLAK ILFMLKDMGV IDISLAAQAP DLFDNTAQQV HHRRRVAFER LEYIGDNSWG NHLSNRMMLL FPDRQWTYSQ NAYTFNCFRD ACEMNVTLEF MFDTLRVGEL LPPGVREKLG TGKIKADVVE AVIGELHVTL WGLEPQLYDS VCFVEINGVG EARLAALVQH CLTEIYDLIV LSYVQELSGS AVPLAKQIAA DRIWNSVYPP VRKAKDRAPG GRKSRSTVVN VVGVGEVRQL PSLPSLFPVA SKRPTRAPHP LRRLRKLGEI PEETVCAGTN KDVFVHLIES YERLDMLDDS LLPTLNMRRL QDVQFSKLKR QLVPSLSPAA LEELHNCKEL DAESVTPHKV GDKEVATDER VLDLEEVYFR DKYFDLFPTP LPNEVVTKEG DCDKRQSGAS TSRAVIISLP RGFDGGRPCN RAQLHMARNT IYPSLTERPA HSPTAGITKG DRVCLFSLSV YVPPDTKRPS AGVVTDKNLH FGEFAFHGVR IKGIECASEP ECISRRSNAP GSDVGTSLNR SSWKEGGDNS CGTLTVEDGT GENSVVGEDG DTGVNGGEGK AEDATGKHLL RAKWCRENPF FPRASLPLLG ISVGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggc tacattggcc cctggcgcgt agttccaact ggtgccacat gcgggcgttg agtaacgaac atttgcggcg ccgggttggc gccgccgtgc gcagcattgc ctcaacggca ccctgttttg ctttagaacg ggttgaggtg gcgcattcgc atgcttattc ccgccgaggc gtgacagtgg gctttacgtc atcttcgtcc cctagcggca aaactggcag tggtgggctc tcgccagcat cacagtcgtt gcattcatcg tcatcttttg atgcgagtaa tggggatgtg ctagccaatt acgtcaactt cgttgcagat gacgctaccg gacctgctgc gaccaaagag aacggtgcgc agccaatgcg tagtttgagg tccgcctctg ctgtcgaaaa tcttctgttt accgacgcag cgaagaacta ttgccgtctc tgcatggagg aggtgcaggt gacgcccaag gcgcatatta gcactcctta ccgtgggagt cacacaaatc acacctgtcg ggaggtcgta cttgactctc tcgctttgct tgccattcgg gggtacccta tcgatgatgt ttacttcgtg tgggcggaca cgctctatca atcatctgtc tttcaacgca tcccagagct cgtctccccg cgttggactg ttgataagcg gagtgaagta ttggctaaga ttctatttat gttaaaagac atgggcgtta tcgatatttc attagccgcc caggcacccg atttgtttga taacacggca caacaagtgc atcaccgccg gcgtgtggcg ttcgaaagat tagaatacat cggtgataac tcgtggggaa accatctttc caatcgtatg atgctcctat ttcccgatcg ccagtggact tacagccaaa acgcctacac cttcaactgc tttcgtgatg cgtgcgagat gaatgtgacg ttggaattta tgttcgatac tctacgtgtg ggggagttac ttccacctgg tgtacgggaa aaattgggaa cggggaagat aaaagcagat gtggtggaag ctgttattgg tgagttgcac gtgacattgt gggggcttga gccgcagctg tatgattctg tgtgctttgt tgaaattaac ggcgtgggag aggcccgtct ggcagcgttg gtgcagcact gtctcacaga aatatatgac cttatcgtgc tttcttacgt tcaggaactc agtggatctg cggttccgtt agcaaagcag atagctgccg atcgcatatg gaacagcgtg tatcctcccg tacgaaaggc gaaggaccgt gcacctgggg gacgaaaaag tcgatctact gttgtgaacg tagtcggtgt aggggaggta cggcaacttc cgtcacttcc ttcgctcttt cctgtagcgt caaagcgacc cacacgggct ccacatccac ttcgccgact taggaagtta ggggagattc ctgaagaaac tgtgtgtgct ggtacgaaca aggatgtttt tgtccatctg atcgagagtt atgaacgtct agatatgttg gatgactctc tccttcccac attgaatatg cgacggctcc aagacgttca attctcaaag ctgaagcgac agttggttcc ctcactctcc cctgccgcgt tggaagaatt gcacaactgt aaagagttgg acgctgaatc ggttacaccg cacaaggttg gcgacaagga ggttgcgacg gatgaacggg tgctggactt agaagaggtt tatttccgtg ataagtattt tgacctcttc ccaactccgt tgccgaatga agttgtcaca aaagagggag actgtgacaa aagacaaagc ggtgcctcca cgagcagggc ggtcatcata tcgctgccac gagggtttga cgggggtcga ccctgcaaca gggcgcaact ccacatggct cgaaacacaa tttatccttc actgactgaa agaccggccc atagtcccac tgctggtatt acgaagggtg atcgtgtgtg tctcttttct ctttcagtct atgtgcctcc agatacgaaa aggccttctg cgggcgtagt gacagacaag aaccttcact ttggtgaatt tgcatttcat ggcgtgcgga tcaagggaat tgaatgtgcc tctgaaccag aatgtattag caggcgaagc aatgctcctg gttccgatgt tgggacctca cttaaccgca gttcatggaa agaaggaggt gacaattcat gtggaacact aactgttgag gatggtaccg gagagaactc ggtagttggg gaagatggcg atactggtgt gaatggcggg gaaggtaagg ctgaagatgc aacaggcaaa cacctactgc gagccaaatg gtgtcgtgaa aatcccttct ttccacgggc gtcgctacct cttttaggca tctcggttgg tgcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat