SSGCID: Target Page

TrbrA.19512.a

KREX2

CENTER ID:	TrbrA.19512.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19512.a.B1.GE40786	full length		1	907
TrbrA.19512.a.B3.GE40788	endo-exonuclease-phosphatase		614	907

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	Tb927.10.3570
RefSeq:	XP_822606.1
UniProt:	Q38BP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRRSRLHLL ADYRTSSKIS DEFNNVLNRS GWGFDHVAVD VGSLSGAAGR LTRDLIGQER DAETVKIIHK QITNVILRRI QPKKSLAIFI DGSEPLWKVR HMRLYPGKKF EGKFYRSAAS PMVYSLEDKL RCVGPDLRTP PKEFIVSGPA APGPVEAKLS AWMLDLATRA LPDGAVTTPT VTGAAAAASA SAGPPEVTFN DTFCLIGGND AFLSALGATP FHNITTVTLQ QGEMKSLSLS EALEWFAMDH LLKGETSIGS GGNELQKRLA SVRTDIVLLY LMANGISATD LPGLGVNFKD LMEAYTAEEA NKLYLFGKTS EERCLRMCPL NLERVLTHAT RRTSTPTRFC QQSADYLEIL LQTHSMICDG GIKNFRWTPN DNDAVPEKQP KIPIERFIGH LKHLAAQGGE TTAVADVGST EPGLAASVDW TFALTGIETL LLSAPKAEMI DQIIPVFTKG HTLPDGVAQD IVDTKNVVEA LHKVRRVLQV VVTSDNSGND DGRIEHPAFN HYPTHYFVRT PGSRGPPPGW TYKSVNLGVR AVALGVRHRV ASGMLTAASR VLDSGGVSSK NSLFVFHVKD GITTPSGGGN EDGWLETPCH ELAGSSANPA SKEISTLRVV TWNVQFSRHS GERTPLGRDG IDWCTSTRYV ALAQTLEGLD ADVIGMQEVE PAWCKYLSQQ PWVREKYAMT CCEHSHAIQP WGVMLLVRRS LCVTSTHHAN VPAFSGHTSV MPEVTVVVSE GVPVTVGSMH LLAPYNQNNI SNRTTQLDNL TKRLRTRPPI NGKQAGLIVM GDFNDCAKNY FTFPPEMGFK DAWLLLHPDE GVSSKSGHTI DGDRNPYAAQ IIEKEFYGRA DRVLFSSRNL QPIQTEIIGT TSVREMGITK QVDIDKDVPE YLYPSDHFGL LVEFQVV

NT Sequence

atgttgcgcc gcagtcgctt gcacctgcta gcggactacc gcacgtcgtc caagatatca gatgaattta acaacgtatt gaatcggagc ggatgggggt ttgatcatgt cgccgtcgac gtcggatctc tctccggcgc ggcaggacgt ttgacacgcg accttattgg ccaggagcgc gatgcggaaa cggtgaaaat tattcacaaa caaatcacaa atgtaatttt gcgtcgtatt caaccgaaga agtcactcgc aattttcatt gacggaagtg agccgttatg gaaagtgcgt cacatgcggt tatatccggg aaagaaattt gagggaaagt tctaccggag tgcagcgtcg cccatggttt attcgcttga agacaaactt cgttgtgtgg gtccggatct gcggacacca ccaaaggagt ttattgtaag tggaccggca gcaccggggc cggtggaggc aaaactctct gcgtggatgc ttgacctcgc gacccgtgca cttccggatg gagcggtaac gacccctaca gttaccggag cggctgcggc tgcttctgcc tcggcagggc cacctgaagt aacattcaac gacacatttt gcctcattgg aggtaatgat gcgtttctat ccgccctcgg cgcaacaccc tttcacaaca tcactaccgt aacacttcag caaggagaga tgaaatccct ttccttgagt gaggcactgg agtggtttgc gatggatcat ctactgaaag gcgaaacatc aatcggttct ggagggaatg aacttcagaa gaggttggct tctgtacgca cagacattgt actcctgtac ttgatggcga atggtattag cgcgacagat cttcccggcc tgggcgtgaa cttcaaggat ttgatggaag cctacactgc agaggaggct aacaaactct acttattcgg gaaaacatct gaagagcgat gtttgcggat gtgccccctt aaccttgagc gtgtactaac ccacgctact cggaggacat caacaccgac tcgtttttgt cagcagtccg cggattacct cgagattctt ctacaaaccc actccatgat atgcgatgga ggcatcaaga attttcggtg gacacccaac gacaatgacg ccgtcccgga gaagcaacct aaaattccca ttgagcgctt tattggtcat ctgaaacatc tggctgcgca gggcggagaa accaccgccg tcgctgatgt cgggagcact gaaccgggtc ttgctgcctc cgtcgattgg acatttgcgc tgacagggat tgagacactg cttttgagcg cccccaaggc agaaatgatc gatcaaatta ttcccgtatt cacaaaaggt cacacacttc ccgatggcgt tgcccaggat atcgtggaca cgaagaacgt cgtggaagct cttcacaaag tgcgtcgtgt gctacaggtt gttgtgacaa gcgacaacag tggtaatgat gacggcagaa tagagcaccc agcctttaac cactatccga ctcactactt tgtgcggacc ccaggttccc gcgggccccc tcctgggtgg acgtacaaaa gtgtcaatct gggggtgcga gccgtcgctc ttggcgtgag gcaccgtgtc gcttcgggaa tgttgacggc ggcttctcga gtgcttgaca gtggtggcgt ctcctccaag aattctctct ttgtatttca tgtgaaggac ggtataacaa cccccagtgg cgggggaaac gaggatggct ggttagagac accgtgccat gagctggctg gctcctctgc aaacccagca tccaaggaga tttcaacgct tcgggtggta acctggaacg tgcagtttag tcgtcacagt ggcgagcgga cgccgttagg gcgtgatgga attgattggt gcacttcaac aaggtacgtg gcactcgccc aaacattgga ggggcttgac gctgatgtta ttggtatgca ggaagtagag ccagcatggt gcaagtatct gtcgcaacaa ccgtgggtac gggagaagta tgccatgact tgctgtgagc atagtcacgc cattcaacct tggggagtca tgttactcgt tagacggtca ctctgcgtaa cgtctacaca ccacgcaaat gttcccgctt tttcaggaca tacgagtgtg atgccggaag tcacagtggt tgtgtctgaa ggtgttccgg tgacggtggg ttcgatgcac cttcttgctc cttataacca gaacaacatc agtaaccgca caactcaact cgacaattta acgaaacgac tacgcacgcg accccctatt aacggtaagc aagcaggatt aattgttatg ggtgatttta atgactgcgc gaagaattac ttcacctttc ccccggaaat gggctttaaa gacgcttggt tacttcttca ccccgatgaa ggtgtgagca gcaaatcagg ccacaccatt gatggcgacc gaaatcctta cgccgcacag attattgaga aagaattcta tggacgcgcg gaccgagtat tgttttcctc tcgaaactta caacctattc aaactgagat tattgggacg acctctgtcc gtgagatggg gatcacaaaa caggtggaca ttgacaagga cgttccggag tatttgtacc cgtctgacca tttcggtctc cttgttgagt ttcaggtggt ttag

Details for TrbrA.19512.a.B1.GE40786

HARVESTED ON:	data unavailable
SEQUENCED ON:	11/22/2016
EXPECTED MW:	100kDa
OBSERVED MW:	100kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML RRSRLHLLAD YRTSSKISDE FNNVLNRSGW GFDHVAVDVG SLSGAAGRLT RDLIGQERDA ETVKIIHKQI TNVILRRIQP KKSLAIFIDG SEPLWKVRHM RLYPGKKFEG KFYRSAASPM VYSLEDKLRC VGPDLRTPPK EFIVSGPAAP GPVEAKLSAW MLDLATRALP DGAVTTPTVT GAAAAASASA GPPEVTFNDT FCLIGGNDAF LSALGATPFH NITTVTLQQG EMKSLSLSEA LEWFAMDHLL KGETSIGSGG NELQKRLASV RTDIVLLYLM ANGISATDLP GLGVNFKDLM EAYTAEEANK LYLFGKTSEE RCLRMCPLNL ERVLTHATRR TSTPTRFCQQ SADYLEILLQ THSMICDGGI KNFRWTPNDN DAVPEKQPKI PIERFIGHLK HLAAQGGETT AVADVGSTEP GLAASVDWTF ALTGIETLLL SAPKAEMIDQ IIPVFTKGHT LPDGVAQDIV DTKNVVEALH KVRRVLQVVV TSDNSGNDDG RIEHPAFNHY PTHYFVRTPG SRGPPPGWTY KSVNLGVRAV ALGVRHRVAS GMLTAASRVL DSGGVSSKNS LFVFHVKDGI TTPSGGGNED GWLETPCHEL AGSSANPASK EISTLRVVTW NVQFSRHSGE RTPLGRDGID WCTSTRYVAL AQTLEGLDAD VIGMQEVEPA WCKYLSQQPW VREKYAMTCC EHSHAIQPWG VMLLVRRSLC VTSTHHANVP AFSGHTSVMP EVTVVVSEGV PVTVGSMHLL APYNQNNISN RTTQLDNLTK RLRTRPPING KQAGLIVMGD FNDCAKNYFT FPPEMGFKDA WLLLHPDEGV SSKSGHTIDG DRNPYAAQII EKEFYGRADR VLFSSRNLQP IQTEIIGTTS VREMGITKQV DIDKDVPEYL YPSDHFGLLV EFQVV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc gccgcagtcg cttgcacctg ctagcggact accgcacgtc gtccaagata tcagatgaat ttaacaacgt attgaatcgg agcggatggg ggtttgatca tgtcgccgtc gacgtcggat ctctctccgg cgcggcagga cgtttgacac gcgaccttat tggccaggag cgcgatgcgg aaacggtgaa aattattcac aaacaaatca caaatgtaat tttgcgtcgt attcaaccga agaagtcact cgcaattttc attgacggaa gtgagccgtt atggaaagtg cgtcacatgc ggttatatcc gggaaagaaa tttgagggaa agttctaccg gagtgcagcg tcgcccatgg tttattcgct tgaagacaaa cttcgttgtg tgggtccgga tctgcggaca ccaccaaagg agtttattgt aagtggaccg gcagcaccgg ggccggtgga ggcaaaactc tctgcgtgga tgcttgacct cgcgacccgt gcacttccgg atggagcggt aacgacccct acagttaccg gagcggctgc ggctgcttct gcctcggcag ggccacctga agtaacattc aacgacacat tttgcctcat tggaggtaat gatgcgtttc tatccgccct cggcgcaaca ccctttcaca acatcactac cgtaacactt cagcaaggag agatgaaatc cctttccttg agtgaggcac tggagtggtt tgcgatggat catctactga aaggcgaaac atcaatcggt tctggaggga atgaacttca gaagaggttg gcttctgtac gcacagacat tgtactcctg tacttgatgg cgaatggtat tagcgcgaca gatcttcccg gcctgggcgt gaacttcaag gatttgatgg aagcctacac tgcagaggag gctaacaaac tctacttatt cgggaaaaca tctgaagagc gatgtttgcg gatgtgcccc cttaaccttg agcgtgtact aacccacgct actcggagga catcaacacc gactcgtttt tgtcagcagt ccgcggatta cctcgagatt cttctacaaa cccactccat gatatgcgat ggaggcatca agaattttcg gtggacaccc aacgacaatg acgccgtccc ggagaagcaa cctaaaattc ccattgagcg ctttattggt catctgaaac atctggctgc gcagggcgga gaaaccaccg ccgtcgctga tgtcgggagc actgaaccgg gtcttgctgc ctccgtcgat tggacatttg cgctgacagg gattgagaca ctgcttttga gcgcccccaa ggcagaaatg atcgatcaaa ttattcccgt attcacaaaa ggtcacacac ttcccgatgg cgttgcccag gatatcgtgg acacgaagaa cgtcgtggaa gctcttcaca aagtgcgtcg tgtgctacag gttgttgtga caagcgacaa cagtggtaat gatgacggca gaatagagca cccagccttt aaccactatc cgactcacta ctttgtgcgg accccaggtt cccgcgggcc ccctcctggg tggacgtaca aaagtgtcaa tctgggggtg cgagccgtcg ctcttggcgt gaggcaccgt gtcgcttcgg gaatgttgac ggcggcttct cgagtgcttg acagtggtgg cgtctcctcc aagaattctc tctttgtatt tcatgtgaag gacggtataa caacccccag tggcggggga aacgaggatg gctggttaga gacaccgtgc catgagctgg ctggctcctc tgcaaaccca gcatccaagg agatttcaac gcttcgggtg gtaacctgga acgtgcagtt tagtcgtcac agtggcgagc ggacgccgtt agggcgtgat ggaattgatt ggtgcacttc aacaaggtac gtggcactcg cccaaacatt ggaggggctt gacgctgatg ttattggtat gcaggaagta gagccagcat ggtgcaagta tctgtcgcaa caaccgtggg tacgggagaa gtatgccatg acttgctgtg agcatagtca cgccattcaa ccttggggag tcatgttact cgttagacgg tcactctgcg taacgtctac acaccacgca aatgttcccg ctttttcagg acatacgagt gtgatgccgg aagtcacagt ggttgtgtct gaaggtgttc cggtgacggt gggttcgatg caccttcttg ctccttataa ccagaacaac atcagtaacc gcacaactca actcgacaat ttaacgaaac gactacgcac gcgaccccct attaacggta agcaagcagg attaattgtt atgggtgatt ttaatgactg cgcgaagaat tacttcacct ttcccccgga aatgggcttt aaagacgctt ggttacttct tcaccccgat gaaggtgtga gcagcaaatc aggccacacc attgatggcg accgaaatcc ttacgccgca cagattattg agaaagaatt ctatggacgc gcggaccgag tattgttttc ctctcgaaac ttacaaccta ttcaaactga gattattggg acgacctctg tccgtgagat ggggatcaca aaacaggtgg acattgacaa ggacgttccg gagtatttgt acccgtctga ccatttcggt ctccttgttg agtttcaggt ggtttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for TrbrA.19512.a.B3.GE40788

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI STLRVVTWNV QFSRHSGERT PLGRDGIDWC TSTRYVALAQ TLEGLDADVI GMQEVEPAWC KYLSQQPWVR EKYAMTCCEH SHAIQPWGVM LLVRRSLCVT STHHANVPAF SGHTSVMPEV TVVVSEGVPV TVGSMHLLAP YNQNNISNRT TQLDNLTKRL RTRPPINGKQ AGLIVMGDFN DCAKNYFTFP PEMGFKDAWL LLHPDEGVSS KSGHTIDGDR NPYAAQIIEK EFYGRADRVL FSSRNLQPIQ TEIIGTTSVR EMGITKQVDI DKDVPEYLYP SDHFGLLVEF QVV

Validated NT Sequence

tcttactcac ttaaaccacc tgaaactcaa caaggagacc gaaatggtca gacgggtaca aatactccgg aacgtccttg tcaatgtcca cctgttttgt gatccccatc tcacggacag aggtcgtccc aataatctca gtttgaatag gttgtaagtt tcgagaggaa aacaatactc ggtccgcgcg tccatagaat tctttctcaa taatctgtgc ggcgtaagga tttcggtcgc catcaatggt gtggcctgat ttgctgctca caccttcatc ggggtgaaga agtaaccaag cgtctttaaa gcccatttcc gggggaaagg tgaagtaatt cttcgcgcag tcattaaaat cacccataac aattaatcct gcttgtttac cgttaatagg gggtcgcgtg cgtagtcgtt tcgttaaatt gtcgagttga gttgtgcggt tactgatgtt gttctggtta taaggagcaa gaaggtgcat cgaacccacc gtcaccggaa caccttcaga cacaaccact gtgacttccg gcatcacact cgtatgtcct gaaaaagcgg gaacatttgc gtggtgtgta gacgttacgc agagtgaccg tctaacgagt aacatgactc cccaaggttg aatggcgtga ctatgctcac agcaagtcat ggcatacttc tcccgtaccc acggttgttg cgacagatac ttgcaccatg ctggctctac ttcctgcata ccaataacat cagcgtcaag cccctctaat gtttgggcga gtgccacgta ccttgttgag gtgcaccaat caattccatc acgccctaac ggcgtccgct cgccactgtg acgactaaac tgcacattcc aggttaccac ccgaagcgtt gaaatcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHIS TLRVVTWNVQ FSRHSGERTP LGRDGIDWCT STRYVALAQT LEGLDADVIG MQEVEPAWCK YLSQQPWVRE KYAMTCCEHS HAIQPWGVML LVRRSLCVTS THHANVPAFS GHTSVMPEVT VVVSEGVPVT VGSMHLLAPY NQNNISNRTT QLDNLTKRLR TRPPINGKQA GLIVMGDFND CAKNYFTFPP EMGFKDAWLL LHPDEGVSSK SGHTIDGDRN PYAAQIIEKE FYGRADRVLF SSRNLQPIQT EIIGTTSVRE MGITKQVDID KDVPEYLYPS DHFGLLVEFQ VV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatttcaa cgcttcgggt ggtaacctgg aacgtgcagt ttagtcgtca cagtggcgag cggacgccgt tagggcgtga tggaattgat tggtgcactt caacaaggta cgtggcactc gcccaaacat tggaggggct tgacgctgat gttattggta tgcaggaagt agagccagca tggtgcaagt atctgtcgca acaaccgtgg gtacgggaga agtatgccat gacttgctgt gagcatagtc acgccattca accttgggga gtcatgttac tcgttagacg gtcactctgc gtaacgtcta cacaccacgc aaatgttccc gctttttcag gacatacgag tgtgatgccg gaagtcacag tggttgtgtc tgaaggtgtt ccggtgacgg tgggttcgat gcaccttctt gctccttata accagaacaa catcagtaac cgcacaactc aactcgacaa tttaacgaaa cgactacgca cgcgaccccc tattaacggt aagcaagcag gattaattgt tatgggtgat tttaatgact gcgcgaagaa ttacttcacc tttcccccgg aaatgggctt taaagacgct tggttacttc ttcaccccga tgaaggtgtg agcagcaaat caggccacac cattgatggc gaccgaaatc cttacgccgc acagattatt gagaaagaat tctatggacg cgcggaccga gtattgtttt cctctcgaaa cttacaacct attcaaactg agattattgg gacgacctct gtccgtgaga tggggatcac aaaacaggtg gacattgaca aggacgttcc ggagtatttg tacccgtctg accatttcgg tctccttgtt gagtttcagg tggtttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat