SSGCID: Target Page

TrbrA.19511.a

KREN3

CENTER ID:	TrbrA.19511.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19511.a.B1.GE40784	full length		1	596
TrbrA.19511.a.B2.GE40785	RNase III-like catalytic-like domain		160	371

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	Tb927.10.5320
RefSeq:	XP_822777.1
UniProt:	Q38B71

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRNSLIQHTR NLSATKQITG IVCDHVDVFT WGHSPPEQHG NKFSSGKATA PFYNAGQRRC TLCDDRLETS FSAHCGYVGH VARVGILERA MEILQKGEKR AANGKSGGLG GKITDLDESI KALVGTWWSR LNDTKREPAL DYKRIPSLSA STTKKRLWRM RFLLQYLRDR GIIRYSLTPA KVAGAGDNAF VRSARFERSE MIGDNIVKVV VPDRLVRLFP ADEGGVTYKL ASIQQLLDSN EGLLEIYDYL GLNNIIGVRL PNNKTKSDVV EALFGELQTF LWASELSCGS CQYPAFPTAE HRYVRALVDH LLNELTHMVI MWRVESTLEN SKEYLAKYLL QGARQNGSFS TSASGATIVK EVDCDRSRYA VLPLLLTFPY STSSANKSAG GKQPPLQSSS LAAAKSVQER GTPRMLFTSP VAMPAVLHSH YNPPLRIVRT IKDYQREILT VLEEEEVVSA LIPCTDSSWG MKEGNMNSRE TGRRKPPHGQ IRAPWHELAL EKYPRWRCDT EEAVVRQALE LRLMQRKVYT KETNWRALTT AMAQRLLPNS PLEVDPVGVR PVRQTGRLLL TFEGTTERRA ALATSMCFPS LAQSAG

NT Sequence

atgcggaact cgctcattca gcatacgcga aacctgtcag cgacaaagca aatcacgggg attgtctgcg accacgtgga tgtcttcact tggggccact cccctcctga acagcacggc aataagtttt cctcagggaa agcaacagcg ccattctaca atgcgggaca aaggcgctgc acactctgtg atgaccgtct agaaacttcc ttctctgctc actgcggcta cgtggggcat gtggcgcgag tgggtatact ggagcgtgca atggaaatac tacaaaaggg ggaaaaacgt gcggcgaatg gaaagagtgg tggtttaggt ggaaaaataa cagatttaga tgaaagcatt aaggcacttg tgggcacctg gtggtctcga ttaaatgata caaagcggga accggcactc gactacaagc gcataccatc actcagcgca tctacgacga aaaagcgatt gtggcgtatg cgtttcctgc tgcagtattt gcgtgataga ggtattatcc gctacagttt gactccggca aaggtagctg gcgcgggaga taacgcgttt gtacgatctg cccgtttcga gagaagtgag atgattggag ataacattgt aaaagttgtg gtccctgacc gcttagttcg gctcttccct gcggatgagg gcggtgtgac atataaattg gcttctattc aacaactgct tgacagtaat gagggattat tggaaatata cgactatctt ggcctcaaca acatcatcgg cgtgaggctt ccaaacaaca agacgaagtc agacgttgtg gaggctttgt ttggtgaact gcagacattt ctttgggcaa gcgagttgtc ctgtgggtcg tgccagtacc ccgcatttcc tactgctgag caccgttatg tgcgcgcgtt agttgatcac ttattgaatg agcttacgca tatggttatt atgtggcgtg ttgagagcac cctcgagaac tctaaggaat atcttgccaa gtaccttcta cagggcgcac gtcaaaatgg tagtttttct actagtgcta gtggtgctac aatcgtaaag gaggtggatt gtgaccgttc ccgctacgct gtcttgccgc tgcttttgac gtttccttac tcgacatcat ctgccaataa atcagcggga ggaaagcagc caccgcttca atcttcgagt ttggctgcag cgaaaagtgt gcaggaaaga gggacgccgc ggatgttgtt tacctcacca gtagctatgc cagccgtttt gcattcgcat tacaacccac cccttcgtat tgtgcgaacc attaaggatt atcagcgtga gattttaacg gtactcgaag aggaggaagt tgtgtctgca ttaatccctt gtacagattc ttcgtggggg atgaaagagg gaaatatgaa ttcgcgggaa actgggagga ggaaaccgcc acatgggcag atccgcgccc catggcacga gttggcactg gaaaaatatc caagatggcg gtgtgacact gaagaagcgg tggtacgcca agccttggag cttcgactca tgcagcggaa ggtttacacc aaagagacca attggagggc attaactacc gccatggctc aacgtctcct tccgaattcc ccattggaag tagatccagt aggtgtacgg cctgttagac aaaccggaag gttgctgttg acttttgaag ggactactga gcgtagagcg gcactcgcta ctagcatgtg ttttccgtcg ctagcacaat cggccggctg a

Details for TrbrA.19511.a.B1.GE40784

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	82
PERCENT COVERAGE:	52

Validated AA Sequence

Validated NT Sequence

cggaaaacac atgctagtag cgagtgccgc cctacgctca gtagtccctt caaaagtcaa cagcaacctt ccggtttgtc taacaggtcg tacacctacc ggatctactt ccaatgggga attcggaagg agacgttgag ccatggcggt agttaatgcc ctccaattgg tctctttggt gtaaaccttc cgctgcatga gtcgaagctc caaggcttgg cgtaccaccg cttcttcagt gtcacaccgc catcttggat atttttccag tgccaactcg tgccatgggg cgcggatctg cccatgtggc ggtttcctcc tcccagtttc ccgcgaattc atatatccct ctttcatccc ccacgaagaa tctgtacaag ggattaatgc agacacaact tcctcctctt cgagtaccgt taaaatctca cgctgataat ccttaatggt tcgcacaata cgaaggggtg ggttgtaatg cgaatgtaaa gcggctggca tagctactgg tgaggtaaac aacatccgcg gcgtccctct ttcctgcaca cttttcgctg cagccaaact cgaagattga agcggtggct gctttcctcc cgctgattta ttggcagatg atgtcgagta aggaaacgtc aaaagcagcg gcaagacagc gtagcgggaa cggtcacaat ccacctcctt tacgattgta gcaccactag cactagtaga aaaactacca ttttgacgtg cgccctgtag aaggtacttg gcaagatatt ccttnnngtt ctcgagggtg ctcnnnnnac gccacataat aaccatatgc gtaagttcat tcaataagtg ntcaactaac gcgcgcacat aannnngctc agcagtagga aatgcgggnn nntnngnnng acccacagga caactcgnnn gnnnnaagaa atgtctgcag nnnnncaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gttgaggccn nnatagtcgt atatttcnnn nnnnnnnnca ttactgtcaa gcagttgttg aatagaagcc aatttatatg tcacaccgcn nncatccgca gggaagagcc gaactaagcg gtcagggacc acaactttta caatgttatc tccaatcatc tcacttctct cgaaacgggc agatcgtaca aacgcgttat ctcccgcgcc agctaccttt gccggagtca aactgtagcg gataatacct ctatcacgca aatactgcag caggaaacgc atacgccaca atcgcttttt cgtcgtagat gcgctgagtg atggtatgcg cttgtagtcg agtgccggtt cccgctttgt atcatttaat cgagaccacc aggtgcccac aagtgcctta atgctttcat ctaaatctgt tatttttcca cctaaaccac cactctttcc attcgccgca cgtttttccc ccttttgtag tatttccatt gcacgctcca gtatacccac tcgcgccaca tgccccacat agccgcagtg agcagagaag gaagtttcta gacggtcatc acagagtgtg cagcgccttt gtcccgcatt gtagaatggt gctgttgctt tccctgagga aaacttattg ccgtgctgtt caggagggga gtggccccaa gtgaagacat ccacgtggtc gcagacaatc cccgtgattt gctttgtcgc tgacaggttt cgcgtatgct gaatgagcga gttccgcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMR NSLIQHTRNL SATKQITGIV CDHVDVFTWG HSPPEQHGNK FSSGKATAPF YNAGQRRCTL CDDRLETSFS AHCGYVGHVA RVGILERAME ILQKGEKRAA NGKSGGLGGK ITDLDESIKA LVGTWWSRLN DTKREPALDY KRIPSLSAST TKKRLWRMRF LLQYLRDRGI IRYSLTPAKV AGAGDNAFVR SARFERSEMI GDNIVKVVVP DRLVRLFPAD EGGVTYKLAS IQQLLDSNEG LLEIYDYLGL NNIIGVRLPN NKTKSDVVEA LFGELQTFLW ASELSCGSCQ YPAFPTAEHR YVRALVDHLL NELTHMVIMW RVESTLENSK EYLAKYLLQG ARQNGSFSTS ASGATIVKEV DCDRSRYAVL PLLLTFPYST SSANKSAGGK QPPLQSSSLA AAKSVQERGT PRMLFTSPVA MPAVLHSHYN PPLRIVRTIK DYQREILTVL EEEEVVSALI PCTDSSWGMK EGNMNSRETG RRKPPHGQIR APWHELALEK YPRWRCDTEE AVVRQALELR LMQRKVYTKE TNWRALTTAM AQRLLPNSPL EVDPVGVRPV RQTGRLLLTF EGTTERRAAL ATSMCFPSLA QSAG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgga actcgctcat tcagcatacg cgaaacctgt cagcgacaaa gcaaatcacg gggattgtct gcgaccacgt ggatgtcttc acttggggcc actcccctcc tgaacagcac ggcaataagt tttcctcagg gaaagcaaca gcgccattct acaatgcggg acaaaggcgc tgcacactct gtgatgaccg tctagaaact tccttctctg ctcactgcgg ctacgtgggg catgtggcgc gagtgggtat actggagcgt gcaatggaaa tactacaaaa gggggaaaaa cgtgcggcga atggaaagag tggtggttta ggtggaaaaa taacagattt agatgaaagc attaaggcac ttgtgggcac ctggtggtct cgattaaatg atacaaagcg ggaaccggca ctcgactaca agcgcatacc atcactcagc gcatctacga cgaaaaagcg attgtggcgt atgcgtttcc tgctgcagta tttgcgtgat agaggtatta tccgctacag tttgactccg gcaaaggtag ctggcgcggg agataacgcg tttgtacgat ctgcccgttt cgagagaagt gagatgattg gagataacat tgtaaaagtt gtggtccctg accgcttagt tcggctcttc cctgcggatg agggcggtgt gacatataaa ttggcttcta ttcaacaact gcttgacagt aatgagggat tattggaaat atacgactat cttggcctca acaacatcat cggcgtgagg cttccaaaca acaagacgaa gtcagacgtt gtggaggctt tgtttggtga actgcagaca tttctttggg caagcgagtt gtcctgtggg tcgtgccagt accccgcatt tcctactgct gagcaccgtt atgtgcgcgc gttagttgat cacttattga atgagcttac gcatatggtt attatgtggc gtgttgagag caccctcgag aactctaagg aatatcttgc caagtacctt ctacagggcg cacgtcaaaa tggtagtttt tctactagtg ctagtggtgc tacaatcgta aaggaggtgg attgtgaccg ttcccgctac gctgtcttgc cgctgctttt gacgtttcct tactcgacat catctgccaa taaatcagcg ggaggaaagc agccaccgct tcaatcttcg agtttggctg cagcgaaaag tgtgcaggaa agagggacgc cgcggatgtt gtttacctca ccagtagcta tgccagccgt tttgcattcg cattacaacc caccccttcg tattgtgcga accattaagg attatcagcg tgagatttta acggtactcg aagaggagga agttgtgtct gcattaatcc cttgtacaga ttcttcgtgg gggatgaaag agggaaatat gaattcgcgg gaaactggga ggaggaaacc gccacatggg cagatccgcg ccccatggca cgagttggca ctggaaaaat atccaagatg gcggtgtgac actgaagaag cggtggtacg ccaagccttg gagcttcgac tcatgcagcg gaaggtttac accaaagaga ccaattggag ggcattaact accgccatgg ctcaacgtct ccttccgaat tccccattgg aagtagatcc agtaggtgta cggcctgtta gacaaaccgg aaggttgctg ttgacttttg aagggactac tgagcgtaga gcggcactcg ctactagcat gtgttttccg tcgctagcac aatcggccgg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for TrbrA.19511.a.B2.GE40785

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR FLLQYLRDRG IIRYSLTPAK VAGAGDNAFV RSARFERSEM IGDNIVKVVV PDRLVRLFPA DEGGVTYKLA SIQQLLDSNE GLLEIYDYLG LNNIIGVRLP NNKTKSDVVE ALFGELQTFL WASELSCGSC QYPAFPTAEH RYVRALVDHL LNELTHMVIM WRVESTLENS KEYLAKYLLQ GARQNGSFST SASGATIVKE VDCDRSRYAV

Validated NT Sequence

atcttactca cttagacagc gtagcgggaa cggtcacaat ccacctcctt tacgattgta gcaccactag cactagtaga aaaactacca ttttgacgtg cgccctgtag aaggtacttg gcaagatatt ccttagagtt ctcgagggtg ctctcaacac gccacataat aaccatatgc gtaagttcat tcaataagtg atcaactaac gcgcgcacat aacggtgctc agcagtagga aatgcggggt actggcacga cccacaggac aactcgcttg cccaaagaaa tgtctgcagt tcaccaaaca aagcctccac aacgtctgac ttcgtcttgt tgtttggaag cctcacgccg atgatgttgt tgaggccaag atagtcgtat atttccaata atccctcatt actgtcaagc agttgttgaa tagaagccaa tttatatgtc acaccgccct catccgcagg gaagagccga actaagcggt cagggaccac aacttttaca atgttatctc caatcatctc acttctctcg aaacgggcag atcgtacaaa cgcgttatct cccgcgccag ctacctttgc cggagtcaaa ctgtagcgga taatacctct atcacgcaaa tactgcagca ggaaacgcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtt tcctgctgca gtatttgcgt gatagaggta ttatccgcta cagtttgact ccggcaaagg tagctggcgc gggagataac gcgtttgtac gatctgcccg tttcgagaga agtgagatga ttggagataa cattgtaaaa gttgtggtcc ctgaccgctt agttcggctc ttccctgcgg atgagggcgg tgtgacatat aaattggctt ctattcaaca actgcttgac agtaatgagg gattattgga aatatacgac tatcttggcc tcaacaacat catcggcgtg aggcttccaa acaacaagac gaagtcagac gttgtggagg ctttgtttgg tgaactgcag acatttcttt gggcaagcga gttgtcctgt gggtcgtgcc agtaccccgc atttcctact gctgagcacc gttatgtgcg cgcgttagtt gatcacttat tgaatgagct tacgcatatg gttattatgt ggcgtgttga gagcaccctc gagaactcta aggaatatct tgccaagtac cttctacagg gcgcacgtca aaatggtagt ttttctacta gtgctagtgg tgctacaatc gtaaaggagg tggattgtga ccgttcccgc tacgctgtct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat