SSGCID: Target Page

TrbrA.19509.a

KREPA2

CENTER ID:	TrbrA.19509.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19509.a.B2.GE40782	C2H2 zinc finger and OB fold		386	587

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	Tb927.10.8210
RefSeq:	XP_823055.1
UniProt:	Q38AE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYRRFFRSAA VKHLGTTSAQ CLMRATKYPC GAMCRNASSC HPQTLGYQQR FQSTTDKKFH CSVCKKPFRL EMAAKLHLQQ AHGGDGSVEA GPGPGVEASS VNVVSTPVPS PISPVERTFV DEEERRPRRM RPTPKPLHQP DRDIPAAAME EMLGVWDKIG LNRLEGNFVH STMVMKVFAA PPDVSEIPLY EHVAPEGENP FDSLDHMTTG TVDATYVGHD AFAEVDLVDP FVAAPDQTLN PFRAGKVRNP FTRISPTQRE VVKPLQPPQP KEEPLKAPVT PFGQLPMFGQ TREPSASFAA AAVSVQAAAN PNEVSSPFAA AVSSSPFVGQ VASPFAPAQD VAGSPFEASP CAASSPFVTA GGQETSPFAA PSTPASFGQG SLFPPMGTGA PGFTAITETE QRQQELEHGC PTCGKKFSTF EGAAMHSKSK HGIVLESKKV KDRLNRKGVP DLPAYVPSPV DLSSTSPFGT RSAIGASWAE TELIPHAQCV SNITIVGRVL DVSQASENVS HVTVFVEGER SGEEETLTLC CFGEVSQKIR GTLKRNATIF ASGTLRLHPV YEASNNKYYV SPVVHVSMPT GTLAVIT

NT Sequence

atgtaccgca ggttctttcg cagcgcagcc gttaagcatt taggcacgac gagtgcgcaa tgtctgatgc gtgcaacaaa atatccctgt ggcgcgatgt gcaggaatgc tagcagctgc cacccacaga cactgggcta ccaacagagg ttccaatcaa caacggacaa gaagtttcac tgtagcgtgt gtaagaagcc atttcgtctt gaaatggcgg ctaagttgca cctccagcag gcgcatggcg gagacggttc tgtggaggcc ggccccggac ccggtgtcga ggcctcatcc gttaacgttg tcagcacacc ggtgccctca ccgataagtc ctgttgagcg aacgtttgtc gatgaagaag agcgccgccc gcgtcgcatg aggccgacgc cgaagcctct tcatcaaccc gaccgtgaca taccagctgc tgcgatggaa gagatgcttg gcgtatggga caaaattggg ttgaacaggc tggagggtaa ctttgtgcac agcacgatgg tgatgaaggt gtttgctgcg ccccctgatg tgtcagagat acccctttat gaacacgttg cccctgaggg cgagaatcca ttcgatagtt tggaccacat gaccacaggt accgtagatg cgacgtacgt tggtcatgat gctttcgcag aagtggacct ggtggaccct tttgttgccg cacccgatca aacattgaac ccatttcgcg ctggaaaggt ccgaaaccct tttacgcgaa tctctcccac acaacgggag gtagtcaagc cattgcaacc gccccaaccg aaagaggagc cacttaaagc ccctgttaca ccgtttgggc agctgccgat gtttgggcag accagggagc caagtgcctc atttgcagca gcagctgttt cggtccaagc ggccgcaaat ccgaatgagg tttcttcacc attcgcagct gcagtcagta gttcaccctt tgttggtcag gttgcgtcgc catttgctcc tgcgcaggat gtggcgggga gcccattcga ggcgtcaccg tgcgcggcct cgtcaccctt tgttacagcc ggagggcagg aaacatctcc ctttgcagct ccgtctacac ctgcctcctt cggtcagggg agcttgtttc ctccaatggg gacgggagcg cctggtttta ctgcaattac tgagactgag cagcgccaac aggagctgga gcacggctgc cccacatgcg gaaaaaaatt ttctacgttt gagggagctg ccatgcacag caagtcaaaa cacggaattg tgctagaatc taagaaggtg aaagacaggc tgaacagaaa gggagttcct gatttacccg cgtacgtgcc cagtccggtg gatttgtctt ccacctcccc ctttggaacc aggtctgcca tcggagcttc atgggccgaa acggagctca tcccccatgc gcagtgcgtg agtaacatca ctattgttgg ccgcgtgctc gatgtttcgc aggcgtcgga gaatgtgtct cacgtgactg tttttgttga aggagagcgg agtggtgagg aggagacctt gacactttgt tgctttggcg aggtctctca aaaaattcgt gggactttga agcgcaacgc aacgatattc gcaagcggca cccttcggct tcatcctgtg tacgaagcat caaacaacaa atattacgtg agtcctgtgg tgcatgtgtc aatgcccaca gggactctag ctgtgatcac atag

Details for TrbrA.19509.a.B2.GE40782

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TGAPGFTAIT ETEQRQQELE HGCPTCGKKF STFEGAAMHS KSKHGIVLES KKVKDRLNRK GVPDLPAYVP SPVDLSSTSP FGTRSAIGAS WAETELIPHA QCVSNITIVG RVLDVSQASE NVSHVTVFVE GERSGEEETL TLCCFGEVSQ KIRGTLKRNA TIFASGTLRL HPVYEASNNK YYVSPVVHVS MPTGTLAVIT

Validated NT Sequence

atcttactca cttatgtgat cacagctaga gtccctgtgg gcattgacac atgcaccaca ggactcacgt aatatttgtt gtttgatgct tcgtacacag gatgaagccg aagggtgccg cttgcgaata tcgttgcgtt gcgcttcaaa gtcccacgaa ttttttgaga gacctcgcca aagcaacaaa gtgtcaaggt ctcctcctca ccactccgct ctccttcaac aaaaacagtc acgtgagaca cattctccga cgcctgcgaa acatcgagca cgcggccaac aatagtgatg ttactcacgc actgcgcatg ggggatgagc tccgtttcgg cccatgaagc tccgatggca gacctggttc caaaggggga ggtggaagac aaatccaccg gactgggcac gtacgcgggt aaatcaggaa ctccctttct gttcagcctg tctttcacct tcttagattc tagcacaatt ccgtgttttg acttgctgtg catggcagct ccctcaaacg tagaaaattt ttttccgcat gtggggcagc cgtgctccag ctcctgttgg cgctgctcag tctcagtaat tgcagtaaaa ccaggcgctc ccgtccccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga cgggagcgcc tggttttact gcaattactg agactgagca gcgccaacag gagctggagc acggctgccc cacatgcgga aaaaaatttt ctacgtttga gggagctgcc atgcacagca agtcaaaaca cggaattgtg ctagaatcta agaaggtgaa agacaggctg aacagaaagg gagttcctga tttacccgcg tacgtgccca gtccggtgga tttgtcttcc acctccccct ttggaaccag gtctgccatc ggagcttcat gggccgaaac ggagctcatc ccccatgcgc agtgcgtgag taacatcact attgttggcc gcgtgctcga tgtttcgcag gcgtcggaga atgtgtctca cgtgactgtt tttgttgaag gagagcggag tggtgaggag gagaccttga cactttgttg ctttggcgag gtctctcaaa aaattcgtgg gactttgaag cgcaacgcaa cgatattcgc aagcggcacc cttcggcttc atcctgtgta cgaagcatca aacaacaaat attacgtgag tcctgtggtg catgtgtcaa tgcccacagg gactctagct gtgatcacat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat