TrbrA.19508.a

KREPB4

CENTER ID: TrbrA.19508.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.19508.a.B1.GE40780 full length 1 414
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: Tb927.11.2990
RefSeq: XP_828381.1
UniProt: Q386L2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRVENLRRS MTRLARHSLI RAVPFSPLSV GSSTDQTFGA PTRTPFRSHT CLLCDVSYES WGDHAESTTH IARHAICRTF VSPERHNAVM QQLWKHIRLD FGYVDEVTHK KEDRRRMRLA STMRHLQEKG VLHHSLPRVT VDAQSEVSLT VESDSFVNYM FLGESFARQE TLDRVARLMP RAEALELSSI ISFVLSKRRL AHFFDILDMR KMVLNGDSSD DKASADGGVP PTIPRLQQDG KAVILFSCLG ELQMFSRRDR SHSVATRSAA EQLVLNVLGT HVMENIIGEL VHEALQTVVE EGTAVWREHC GELKHKLFEG TKAVSPPIAT TPNPVSNSGG PEVTADVNDQ MWVDLCRLYV LDKNGSVPQL QPTVKRHSWH DVARALTLEL TVPNPVNKSA VFAAAAPRLA TKKK
NT Sequence
atgttacgtg ttgaaaactt gcggcgttcg atgactcgcc tggctcgtca ttctctcatt cgtgccgtcc ccttttcgcc tctgagtgtc ggatccagta ccgaccaaac ctttggtgcc cccacccgaa caccattccg gtctcacacg tgccttcttt gcgatgtgtc gtacgagtcg tggggagacc acgcagagag taccactcac atagcgcgtc atgctatttg caggactttt gtatcccctg agcgacataa tgcggtaatg caacagctgt ggaagcatat tcgtcttgat tttgggtacg ttgacgaggt cactcataaa aaggaggacc gtcgacgcat gcgcctcgcc tccacgatgc gccacctaca ggagaaggga gtgcttcacc actcacttcc ccgtgtgacg gtagacgcgc agtccgaggt atcgcttacg gtggagtcag atagctttgt gaattatatg ttcctgggcg aaagctttgc gcggcaggag acgttggatc gcgtggcgcg gctgatgccg cgggcagaag ctcttgaact tagtagtatc atatcgtttg ttctttcgaa gaggcgccta gctcattttt tcgatatact tgacatgcgt aaaatggttt taaatggtga cagttccgac gataaggcgt cggctgatgg tggcgtacca cccaccattc cacgtctgca gcaggacggg aaggctgtta tcctgttcag ctgtttgggg gagttgcaaa tgttctcgcg gcgggatcga tcgcacagcg tggccacacg gtcagccgcg gagcaactgg tcctcaatgt gcttggcaca cacgtaatgg agaacatcat aggcgaactg gtgcacgagg cactgcagac tgtcgtggag gaagggacgg cagtatggcg tgaacactgt ggtgaattga agcacaagtt atttgaaggg acaaaggcgg tatcaccccc catagctacg actccaaacc ccgtgtcaaa ctctggtgga ccagaagtga cagcggacgt taatgatcaa atgtgggtgg acctttgcag attgtacgtg ctcgacaaga atggcagtgt gcctcagctt caacctacgg tgaagcgaca ctcatggcat gatgtggcgc gcgcgctgac actcgaacta acggttccga atccagtgaa caagagcgcc gttttcgctg cggctgctcc ccgccttgcc acaaagaaaa aatag
Details for TrbrA.19508.a.B1.GE40780
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 47kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML RVENLRRSMT RLARHSLIRA VPFSPLSVGS STDQTFGAPT RTPFRSHTCL LCDVSYESWG DHAESTTHIA RHAICRTFVS PERHNAVMQQ LWKHIRLDFG YVDEVTHKKE DRRRMRLAST MRHLQEKGVL HHSLPRVTVD AQSEVSLTVE SDSFVNYMFL GESFARQETL DRVARLMPRA EALELSSIIS FVLSKRRLAH FFDILDMRKM VLNGDSSDDK ASADGDVPPT IPRLQQDGKA VILFSCLGEL QMFSRRDRSH SVATRSAAEQ LVLNVLGTHV MENIIGELVH EALQTVVEEG TAVWREHCGE LKHKLFEGTK AASPPIATTP NPVSNSGGPE VTADVNDQMW VDLCRLYVLD KNGSVPQLQP TVKRHSWHDV ARALTLELTV PNPVNKSAVF AAAAP
Validated NT Sequence
ggggagcagc cgcagcgaaa acggcgctct tgttcactgg attcggaacc gttagttcga gtgtcagcgc gcgcgccaca tcatgccatg agtgtcgctt caccgtaggt tgaagctgag gcacactgcc attcttgtcg agcacgtaca atctgcaaag gtccacccac atttgatcat taacgtccgc cgtcacttct ggtccaccag agtttgacac ggggtttgga gtcgtagcta tggggggtga tgccgccttt gtcccttcaa ataacttgtg cttcaattca ccacagtgtt cacgccatac tgccgtccct tcctccacga cagtctgcag tgcctcgtgc accagttcgc ctatgatgtt ctccattacg tgtgtgccaa gcacattgag gaccagttgc tccgcggctg accgtgtggc cacgctgtgc gatcgatccc gccgcgagaa catttgcaac tcccccaaac agctgaacag gataacagcc ttcccgtcct gctgcagacg tggaatggtg ggtggtacgt caccatcagc cgacgcctta tcgtcggaac tgtcaccatt taaaaccatt ttacgcatgt caagtatatc gaaaaaatga gctaggcgcc tcttcgaaag aacaaacgat atgatactac taagttcaag agcttctgcc cgcggcatca gccgcgccac gcgatccaac gtctcctgcc gcgcaaagct ttcgccgagg aacatataat tcacaaagct atctgactcc accgtaagcg atacctcgga ctgcgcgtct accgtcacac ggggaagtga gtggtgaagc actcccttct cctgtaggtg gcgcatcgtg gaggcgaggc gcatgcgtcg acggtcctcc tttttatgag tgacctcgtc aacgtaccca aaatcaagac gaatatgctt ccacagctgt tgcattaccg cattatgtcg ctcaggggat acaaaagtcc tgcaaatagc atgacgcgct atgtgagtgg tactctctgc gtggtctccc cacgactcgt acgacacatc gcaaagaagg cacgtgtgag accggaatgg tgttcgggtg ggggcaccaa aggtttggtc ggtactggat ccgacactca gaggcgaaaa ggggacggca cgaatgagag aatgacgagc caggcgagtc atcgaacgcc gcaagttttc aacacgtaac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHML RVENLRRSMT RLARHSLIRA VPFSPLSVGS STDQTFGAPT RTPFRSHTCL LCDVSYESWG DHAESTTHIA RHAICRTFVS PERHNAVMQQ LWKHIRLDFG YVDEVTHKKE DRRRMRLAST MRHLQEKGVL HHSLPRVTVD AQSEVSLTVE SDSFVNYMFL GESFARQETL DRVARLMPRA EALELSSIIS FVLSKRRLAH FFDILDMRKM VLNGDSSDDK ASADGGVPPT IPRLQQDGKA VILFSCLGEL QMFSRRDRSH SVATRSAAEQ LVLNVLGTHV MENIIGELVH EALQTVVEEG TAVWREHCGE LKHKLFEGTK AVSPPIATTP NPVSNSGGPE VTADVNDQMW VDLCRLYVLD KNGSVPQLQP TVKRHSWHDV ARALTLELTV PNPVNKSAVF AAAAPRLATK KK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttac gtgttgaaaa cttgcggcgt tcgatgactc gcctggctcg tcattctctc attcgtgccg tccccttttc gcctctgagt gtcggatcca gtaccgacca aacctttggt gcccccaccc gaacaccatt ccggtctcac acgtgccttc tttgcgatgt gtcgtacgag tcgtggggag accacgcaga gagtaccact cacatagcgc gtcatgctat ttgcaggact tttgtatccc ctgagcgaca taatgcggta atgcaacagc tgtggaagca tattcgtctt gattttgggt acgttgacga ggtcactcat aaaaaggagg accgtcgacg catgcgcctc gcctccacga tgcgccacct acaggagaag ggagtgcttc accactcact tccccgtgtg acggtagacg cgcagtccga ggtatcgctt acggtggagt cagatagctt tgtgaattat atgttcctgg gcgaaagctt tgcgcggcag gagacgttgg atcgcgtggc gcggctgatg ccgcgggcag aagctcttga acttagtagt atcatatcgt ttgttctttc gaagaggcgc ctagctcatt ttttcgatat acttgacatg cgtaaaatgg ttttaaatgg tgacagttcc gacgataagg cgtcggctga tggtggcgta ccacccacca ttccacgtct gcagcaggac gggaaggctg ttatcctgtt cagctgtttg ggggagttgc aaatgttctc gcggcgggat cgatcgcaca gcgtggccac acggtcagcc gcggagcaac tggtcctcaa tgtgcttggc acacacgtaa tggagaacat cataggcgaa ctggtgcacg aggcactgca gactgtcgtg gaggaaggga cggcagtatg gcgtgaacac tgtggtgaat tgaagcacaa gttatttgaa gggacaaagg cggtatcacc ccccatagct acgactccaa accccgtgtc aaactctggt ggaccagaag tgacagcgga cgttaatgat caaatgtggg tggacctttg cagattgtac gtgctcgaca agaatggcag tgtgcctcag cttcaaccta cggtgaagcg acactcatgg catgatgtgg cgcgcgcgct gacactcgaa ctaacggttc cgaatccagt gaacaagagc gccgttttcg ctgcggctgc tccccgcctt gccacaaaga aaaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat