SSGCID: Target Page

TrbrA.19506.a

KREPB6

CENTER ID:	TrbrA.19506.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19506.a.B1.GE40776	full length		1	438
TrbrA.19506.a.B2.GE40777	ordered domain 1		54	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	Tb927.3.3990
RefSeq:	XP_843991.1
UniProt:	Q57WD5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTPVFGTAAF FCRLRSSSLR RFCLNHQRRA RSSGSSIGIA HEVTSLHPYG CSNNVVGSTR VWNETENWCS LCLEPLNSWS EHRGKRDHIC LEMFYNAITQ YGRRWSPSTL WWEVEECTLL RRHASPRLVL DGGEGFFSGC ILDYVRDSTG KRGGLSLLMD YYDRMEHYKR RLELYACIQH LIDNGIVRTD RGSLGGGASF HGSLVTFKEL FPNLVNMFPF ADAKEISALT QMIASTYNGE TVFDLCNFQG LLPAGQGGGD TTGGKGNTSG CGNSQQRLAF NAEQKGSEDS SVTASAEGVY SYHWKGMFCR GVMGQLRWAL ECDSVACPLK RADGSKHVCD PYWQVLSEYT CRALLAEMVF CRVSEYIVRV EGVWRKCGQE LANKHVSHGS QKEEEERAHP PRTSNWGYIR YMGDHLYDDM FGGNLPNEGA PKRLSLMR

NT Sequence

atgactcctg tatttggtac cgctgctttc ttttgtaggt tgcggtcctc gtctctgcga cgattttgcc tcaatcatca acgcagggct cgcagtagcg gcagttcaat tggtattgct catgaagtta cttcacttca cccatatggt tgcagcaaca atgttgttgg ttctacacgg gtttggaatg aaacggagaa ttggtgcagt ctttgcttgg agccacttaa cagttggagc gagcaccgcg ggaagcgcga tcacatttgt ttggagatgt tttataatgc gataactcaa tacgggcgcc gctggtcacc atcgacatta tggtgggaag tggaggaatg cactttgctg cgtcgccatg cttctccgcg acttgtgttg gatggtggcg agggtttttt tagtggttgt attttggatt atgtacgcga ctcaactgga aagaggggag gtcttagtct tttgatggat tattacgatc gaatggagca ttacaagcgg cgtttggagc tctacgcttg cattcaacat ttgattgaca atggtatcgt gcgcaccgat cggggttcgc tgggcggcgg cgcgtcattc cacggttctc tcgtgacatt caaggaatta tttccaaatt tagtaaatat gtttccattt gcggacgcaa aggagatctc cgccttaaca caaatgatag cctcgacgta taacggtgaa acggtattcg acttgtgtaa cttccagggg ttgcttccag cgggtcaagg aggaggcgat acaacaggag gaaaaggcaa tacttcagga tgcggaaact cgcagcagcg actggcattc aacgccgaac aaaagggaag tgaagattcg tccgttaccg catctgctga gggggtttat tcatatcatt ggaaaggaat gttttgtcgc ggcgtgatgg gtcaattacg atgggcattg gagtgtgata gcgttgcctg cccactgaaa cgtgccgatg gctccaagca cgtttgcgac ccctattggc aagttctttc ggaatacacc tgtcgggccc tccttgcgga aatggttttt tgtcgtgttt cggagtatat cgtgcgcgtt gagggtgttt ggagaaaatg tggtcaggaa ttggcgaaca aacatgtgtc gcatgggagt caaaaggaag aagaggagag ggcgcatccg ccgcgcacgt cgaattgggg ttacatacga tacatggggg atcatttgta tgatgacatg tttgggggaa acttgccaaa tgaaggggct ccgaagagat tgtctttgat gaggtga

Details for TrbrA.19506.a.B1.GE40776

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT PFGTAAFFCR LRSSSLRRFC LNHQRRARSS GSSIGIAHEV TSLHPYGCSN NVVGSTRVWN ETENWCSLCL EPLNSWSEHR GKRDHICLEM FYNAITQYGR RWSPSTLWWE VEECTLLRRH ASPRLVLDGG EGFFSGCILD YVRDSTGKRG GLSLLMDYYD RMEHYKRRLE LYACIQHLID NGIVRTDRGS LGGGASFHGS LVTFKELFPN LVNMFPFADA KEISALTQMI ASTYNGETVF DLCNFQGLLP AGQGGGDTTG GKGNTSGCGN SQQRLAFNAE QKGSEDSSVT ASAEGVYSYH WKGMFCRGVM GQLRWALECD SVACPLKRAD GSKHVCDPYW QVLSEYTCRA LLAEMVFCRV SEYIVRVEGV WRKCGQELAN KHVSHGSQKE EEERAHPPRT SNWGYIRYMG DHLYDDMFGG NLPNEGA

Validated NT Sequence

cggagcccct tcatttggca agtttccccc aaacatgtca tcatacaaat gatcccccat gtatcgtatg taaccccaat tcgacgtgcg cggcggatgc gccctctcct cttcttcctt ttgactccca tgcgacacat gtttgttcgc caattcctga ccacattttc tccaaacacc ctcaacgcgc acgatatact ccgaaacacg acaaaaaacc atttccgcaa ggagggcccg acaggtgtat tccgaaagaa cttgccaata ggggtcgcaa acgtgcttgg agccatcggc acgtttcagt gggcaggcaa cgctatcaca ctccaatgcc catcgtaatt gacccatcac gccgcgacaa aacattcctt tccaatgata tgaataaacc ccctcagcag atgcggtaac ggacgaatct tcacttccct tttgttcggc gttgaatgcc agtcgctgct gcgagtttcc gcatcctgaa gtattgcctt ttcctcctgt tgtatcgcct cctccttgac ccgctggaag caacccctgg aagttacaca agtcgaatac cgtttcaccg ttgtacgtcg aggctatcat ttgtgttaag gcggagatct cctttgcgtc cgcaaatgga aacatattta ctaaatttgg aaataattcc ttgaatgtca cgagagaacc gtggaatgac gcgccgccgc ccagcgaacc ccgatcggtg cgcacgatac cattgtcaat caaatgttga atgcaagcgt agagctccaa acgccgcttg taatgctcca ttcgatcgta ataatccatc aaaagactaa gacctcccct ctttccagtt gagtcgcgta cataatccaa aatacaacca ctaaaaaaac cctcgccacc atccaacaca agtcgcggag aagcatggcg acgcagcaaa gtgcattcct ccacttccca ccataatgtc gatggtgacc agcggcgccc gtattgagtt atcgcattat aaaacatctc caaacaaatg tgatcgcgct tcccgcggtg ctcgctccaa ctgttaagtg gctccaagca aagactgcac caattctccg tttcattcca aacccgtgta gaaccaacaa cattgttgct gcaaccatat gggtgaagtg aagtaacttc atgagcaata ccaattgaac tgccgctact gcgagccctg cgttgatgat tgaggcaaaa tcgtcgcaga gacgaggacc gcaacctaca aaagaaagca gcggtaccaa aaggagtcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHMT PVFGTAAFFC RLRSSSLRRF CLNHQRRARS SGSSIGIAHE VTSLHPYGCS NNVVGSTRVW NETENWCSLC LEPLNSWSEH RGKRDHICLE MFYNAITQYG RRWSPSTLWW EVEECTLLRR HASPRLVLDG GEGFFSGCIL DYVRDSTGKR GGLSLLMDYY DRMEHYKRRL ELYACIQHLI DNGIVRTDRG SLGGGASFHG SLVTFKELFP NLVNMFPFAD AKEISALTQM IASTYNGETV FDLCNFQGLL PAGQGGGDTT GGKGNTSGCG NSQQRLAFNA EQKGSEDSSV TASAEGVYSY HWKGMFCRGV MGQLRWALEC DSVACPLKRA DGSKHVCDPY WQVLSEYTCR ALLAEMVFCR VSEYIVRVEG VWRKCGQELA NKHVSHGSQK EEEERAHPPR TSNWGYIRYM GDHLYDDMFG GNLPNEGAPK RLSLMR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactc ctgtatttgg taccgctgct ttcttttgta ggttgcggtc ctcgtctctg cgacgatttt gcctcaatca tcaacgcagg gctcgcagta gcggcagttc aattggtatt gctcatgaag ttacttcact tcacccatat ggttgcagca acaatgttgt tggttctaca cgggtttgga atgaaacgga gaattggtgc agtctttgct tggagccact taacagttgg agcgagcacc gcgggaagcg cgatcacatt tgtttggaga tgttttataa tgcgataact caatacgggc gccgctggtc accatcgaca ttatggtggg aagtggagga atgcactttg ctgcgtcgcc atgcttctcc gcgacttgtg ttggatggtg gcgagggttt ttttagtggt tgtattttgg attatgtacg cgactcaact ggaaagaggg gaggtcttag tcttttgatg gattattacg atcgaatgga gcattacaag cggcgtttgg agctctacgc ttgcattcaa catttgattg acaatggtat cgtgcgcacc gatcggggtt cgctgggcgg cggcgcgtca ttccacggtt ctctcgtgac attcaaggaa ttatttccaa atttagtaaa tatgtttcca tttgcggacg caaaggagat ctccgcctta acacaaatga tagcctcgac gtataacggt gaaacggtat tcgacttgtg taacttccag gggttgcttc cagcgggtca aggaggaggc gatacaacag gaggaaaagg caatacttca ggatgcggaa actcgcagca gcgactggca ttcaacgccg aacaaaaggg aagtgaagat tcgtccgtta ccgcatctgc tgagggggtt tattcatatc attggaaagg aatgttttgt cgcggcgtga tgggtcaatt acgatgggca ttggagtgtg atagcgttgc ctgcccactg aaacgtgccg atggctccaa gcacgtttgc gacccctatt ggcaagttct ttcggaatac acctgtcggg ccctccttgc ggaaatggtt ttttgtcgtg tttcggagta tatcgtgcgc gttgagggtg tttggagaaa atgtggtcag gaattggcga acaaacatgt gtcgcatggg agtcaaaagg aagaagagga gagggcgcat ccgccgcgca cgtcgaattg gggttacata cgatacatgg gggatcattt gtatgatgac atgtttgggg gaaacttgcc aaatgaaggg gctccgaaga gattgtcttt gatgaggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for TrbrA.19506.a.B2.GE40777

HARVESTED ON:	data unavailable
SEQUENCED ON:	11/22/2016
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN VVGSTRVWNE TENWCSLCLE PLNSWSEHRG KRDHICLEMF YNAITQYGRR WSPSTLWWEV EECTLLRRHA SPRLVLDGGE GFFSGCILDY VRDSTGKRGG LSLLMDYYDR MEHYKRRLEL YACIQHLIDN GIVRTDRGSL GGGASFHGSL VTFKELFPNL VNMFPFADAK EISALTQMIA STYNGETVFD LCNFQGL

Validated NT Sequence

atcttactca cttacaaccc ctggaagtta cacaagtcga ataccgtttc accgttgtac gtcgaggcta tcatttgtgt taaggcggag atctcctttg cgtccgcaaa tggaaacata tttactaaat ttggaaataa ttccttgaat gtcacgagag aaccgtggaa tgacgcgccg ccgcccagcg aaccccgatc ggtgcgcacg ataccattgt caatcaaatg ttgaatgcaa gcgtagagct ccaaacgccg cttgtaatgc tccattcgat cgtaataatc catcaaaaga ctaagacctc ccctctttcc agttgagtcg cgtacataat ccaaaataca accactaaaa aaaccctcgc caccatccaa cacaagtcgc ggagaagcat ggcgacgcag caaagtgcat tcctccactt cccaccataa tgtcgatggt gaccagcggc gcccgtattg agttatcgca ttataaaaca tctccaaaca aatgtgatcg cgcttcccgc ggtgctcgct ccaactgtta agtggctcca agcaaagact gcaccaattc tccgtttcat tccaaacccg tgtagaacca acaacattca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHNV VGSTRVWNET ENWCSLCLEP LNSWSEHRGK RDHICLEMFY NAITQYGRRW SPSTLWWEVE ECTLLRRHAS PRLVLDGGEG FFSGCILDYV RDSTGKRGGL SLLMDYYDRM EHYKRRLELY ACIQHLIDNG IVRTDRGSLG GGASFHGSLV TFKELFPNLV NMFPFADAKE ISALTQMIAS TYNGETVFDL CNFQGL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatgttg ttggttctac acgggtttgg aatgaaacgg agaattggtg cagtctttgc ttggagccac ttaacagttg gagcgagcac cgcgggaagc gcgatcacat ttgtttggag atgttttata atgcgataac tcaatacggg cgccgctggt caccatcgac attatggtgg gaagtggagg aatgcacttt gctgcgtcgc catgcttctc cgcgacttgt gttggatggt ggcgagggtt tttttagtgg ttgtattttg gattatgtac gcgactcaac tggaaagagg ggaggtctta gtcttttgat ggattattac gatcgaatgg agcattacaa gcggcgtttg gagctctacg cttgcattca acatttgatt gacaatggta tcgtgcgcac cgatcggggt tcgctgggcg gcggcgcgtc attccacggt tctctcgtga cattcaagga attatttcca aatttagtaa atatgtttcc atttgcggac gcaaaggaga tctccgcctt aacacaaatg atagcctcga cgtataacgg tgaaacggta ttcgacttgt gtaacttcca ggggttgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at