TrbrA.19505.a

KREX1

CENTER ID: TrbrA.19505.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.19505.a.B2.GE40774 5'-3' exonuclease 1 539
TrbrA.19505.a.B3.GE40775 endo-exonuclease-phosphatase 617 890
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: Tb927.7.1070
RefSeq: XP_845731.1
UniProt: Q57WU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALAQSCRHF AATTYRQSKI TDRFQLLITK KEIECDYVAV DATALVATGL RLAKNVTTEQ RRHKEVARHV VQSIQQLLKK VRCKKSLLIA MDGAENLLKA DRTRGSSLTR KVESRLMRLP GTPLMQAVEE RIVRMMPERQ ILPGEVVFAG TCVQGCVEQK MSAWALDLAS RDTFNGSSDS LNLIGASELY LNVLALSPFY NVSSVVQNNA DLRHIRLQDI LEWLELDKKA KEGESVTIAK MRTDILFLFI IANGASATEL NPIPAVGFHE IVDRYMKVLA ENGATPPSQS ATASAPTASA NVPAAPAFLF EDSPGNTLQL NLRMLCRIMQ LVARKEAPAR VDGLSEAYLE HALQTHAMLC TGQAPQCTYL PAAGTGQLGS TAPSAVQLAG HLAALCASTA QRNARCTSLQ PSLVTPEKGS CEEDSSATKL PPTPPTSAVA PVEDKPLTAA EYTILCQSLP AAVEGLIHQY VGVAPKPDVG KMITTATTEE AYRLVREVLS YANPLRPHKC LCLSPSYCWL QNEKTQLWRF EYVDIGVKSH ELGTRRQLNA MKGVTLEVNM SRDGPSHFDP LSGTWEPIAN FPCGAECEYD KHTEECTSSP SAPQTAKEVE GGPVKASLKL LTWNVMFDRY SGKPTPLGMP GIDWCSPKRY PVIAKIIEEE DADVVGMQEV EPVFWEFLSK RPLIRQRYYF SCGHMSPAIA PWGVLMLIHR RRLPVQSINY LNVPAWTNHV SLMPVVGLKM VHGTVHIAAA HLLAPYTKSH ENARTSQDTA LRHHMTKTLG GGDVVTMGDF NDWPTNEFIM PHESQYVDCW PVLHPNNPGK TMDETNTFCK LKVEEMFFGR SDKVFLRSRR LVPVEAHLVG TRSVNDENNN ADAPAYLFPS DHYGVSITFS LKLP
NT Sequence
atggcattgg ctcagtcatg tcgtcacttt gctgcgacga cgtaccgaca gtccaaaatt acagatcgct tccagctgtt aataactaaa aaggaaattg aatgtgacta cgttgcggtg gacgccactg ccctcgtcgc aacggggtta cgtctcgcaa agaacgttac aactgagcag cgtcgccata aggaagtggc gcgtcatgta gtacagtcaa tacaacagtt gttgaagaag gtgcgttgca agaagtctct tcttatagca atggacggag ctgagaatct gctcaaggcg gatcgtacac ggggtagcag tttaacccga aaggtggaaa gtcgtctgat gagactaccg ggaactccac ttatgcaggc agtggaggaa cgtatcgttc gtatgatgcc agagcggcag atattacccg gtgaagtggt gtttgccggc acctgtgttc aagggtgtgt cgagcagaag atgtccgctt gggcacttga tctggcgtcc cgagacacct ttaacggcag cagcgactca ctgaatttga ttggggcctc tgagttgtac cttaatgtgt tggcgctgtc gcccttctat aacgtatcga gtgtggtgca aaataacgca gatttgcgac atatacgact gcaggatatt ttggagtggc tagagctaga taagaaggcg aaggaagggg agagtgtcac aatagccaag atgagaacgg atattttatt cctcttcatt atcgctaacg gcgcctcggc aactgagctg aatcccattc ctgctgttgg tttccatgaa attgttgata ggtacatgaa ggttctcgca gaaaacggtg ctacgccccc ttctcaaagc gcgaccgcga gcgccccaac tgcttcagcc aacgtcccgg ccgccccagc atttcttttt gaggatagtc cgggcaacac actccaactg aatcttcgca tgctctgtcg catcatgcag ttagtggcgc gtaaggaggc gccagcccgt gtggatggtc tgtcagaagc ctacttggag cacgccttac aaacgcatgc aatgttgtgt actggacagg caccccaatg cacctacttg ccagcagcgg gtacaggaca actaggctcg acggcgccta gcgccgtgca gctcgcaggt catcttgctg cattatgcgc atccacagca caacgcaacg cacggtgtac ttctctgcag ccctcgctgg taacaccaga aaagggatcg tgtgaggaag acagttcggc aacaaagctg ccgccgacac ccccaactag cgcggtcgca ccagttgaag acaagccgct gacagcagct gaatacacca ttctctgtca gtcgcttccc gcagctgtgg aaggattgat tcatcagtac gtaggcgtag cgcctaagcc ggatgttggg aagatgatta caacggcgac cacggaggaa gcctaccgcc ttgttcggga agtgctttct tatgcgaacc ctcttcgacc gcataagtgc ttatgccttt ccccatctta ttgctggctc cagaatgaaa agacgcaact gtggcgcttc gaatatgtcg atattggtgt caaaagtcat gaactgggga cacgtcggca actcaatgct atgaagggtg taacgcttga agtgaacatg tcccgtgatg gtccttcgca ttttgatcca ctgagtggga cgtgggagcc aattgccaat ttcccgtgcg gagcggagtg cgaatatgac aaacatactg aagaatgcac atcttctcct tcagctccac aaactgcaaa agaggttgaa ggaggaccag ttaaggcatc tttaaaattg ctgacatgga atgtcatgtt tgatcgttac agcggcaaac caactccttt aggtatgcct ggtatagatt ggtgctcccc caagaggtat cccgtcattg ccaagatcat agaggaggag gatgcggatg ttgttggcat gcaagaggtg gagcctgtat tttgggagtt cctttccaag cggccactta tacgccaaag gtactacttt tcgtgcggtc atatgagtcc agctattgcc ccgtgggggg tgctgatgtt gatacaccgt cggcgtcttc cagtacagag catcaactat cttaacgtcc ctgcgtggac taatcatgtt tctctcatgc cggttgtggg tctgaagatg gtgcacggta ctgtacacat agctgccgca cacttactcg cgccatacac gaagtcccac gagaacgctc gtacttccca ggataccgcg ttacgccatc acatgacgaa gacgttgggt ggcggggatg tggtaactat gggagatttc aacgactggc ctacaaatga atttattatg ccacatgaat cacagtacgt ggactgttgg cccgttttgc accccaacaa ccctggaaag actatggatg aaacaaacac cttttgtaaa ctgaaagtgg aggaaatgtt ttttggccgt tccgacaagg tgtttctgcg aagcaggcgg cttgtgccag tcgaagctca tctcgtcgga acacggagtg taaacgatga gaacaacaac gccgacgcac ccgcatattt gttcccatcc gatcactacg gcgtaagtat aacgttttca ttgaagttac cgtaa
Details for TrbrA.19505.a.B2.GE40774
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 60kDa
OBSERVED MW: 61kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA LAQSCRHFAA TTYRQSKITD RFQLLITKKE IECDYVAVDA TALVATGLRL AKNVTTEQRR HKEVARHVVQ SIQQLLKKVR CKKSLLIAMD GAENLLKADR TRGSSLTRKV ESRLMRLPGT PLMQAVEERI VRMMPERQIL PGEVVFAGTC VQGCVEQKMS AWALDLASRD TFNGSSDSLN LIGASELYLN VLALSPFYNV SSVVQNNADL RHIRLQDILE WLELDKKAXX GESVTIAKMR TDILFLXIIA NGASATELNP IPAVGFHEIV IXXMKVLAEN GATPPSQSAT ASAPTASANL PAAPPFLFED SPGNTLQLNL RMLCRIMQLV ARKEAPARVD GLSEAYLEHA LQTHAMLCTG QAPQCTYLPA AGTGQLGSTA PSAVQLAGHL AALCASTAQR NARCTSLQPS LVTPEKGSCE EDSPATKLPP TPPTSAAAPV EDKPLTAAEY TILCQSLPAA VEGLIHQYVG VAPKPDVGKM ITTATTEEAY RLVREVLSYA NPLRPHKCLC LSPSYCWLQN EKTQLWRFE
Validated NT Sequence
atattcgaag cgccacagtt gcgtcttttc attctggagc cagcaataag atggggaaag gcataagcac ttatgcggtc gaagagggtt cgcataagaa agcacttccc gaacaaggcg gtaggcttcc tccgtggtcg ccgttgtaat catcttccca acatccggct taggcgctac gcctacgtac tgatgaatca atccttccac agctgcggga agcgactgac agagaatggt gtattcagct gctgtcagcg gcttgtcttc aactggtgcg gccgcgctag ttgggggtgt cggcggcagc tttgttgccg gactgtcttc ctcacacgat cccttttctg gtgttaccag cgagggctgc agagaagtac accgtgcatt gcgttgtgct gtggatgcgc ataatgcagc aagatgacct gcgagttgca cggcactagg cgccgtcgag cctagttgtc ctgtacccgc tgctggcaag taggtgcatt ggggtgcctg tccagtgcac aacattgcat gcgtttgtaa ggcgtgctcc aagtaggctt ctgatagacc atccacacgg gctggcgcct ccttacgcgc cactaactgc atgatgcgac agagcatgcg aagattcagt tggagtgtgt tgcccggact atcctcaaaa agaaatggtg gggcggccgg gaggttggct gaagcagttg gggcgctcgc ggtcgcgctt tgagaagggg gcgtagcacc gttttctgcg agaaccttca tgtnnnngat cacaatttca tggaaaccaa cagcaggaat gggattcagc tcagttgccg aggcgccgtt agcgataatg annaggaata aaatatccgt tctcatcttg gctattgtga cactctcccc nnnnnnngcc ttcttatcta gctctagcca ctccaaaata tcctgcagtc gtatatgtcg caaatctgcg ttattttgca ccacactcga tacgttatag aagggcgaca gcgccaacac attaaggtac aactcggagg ccccaatcaa attcagtgaa tcgctgctgc cgttaaaggt gtctcgggac gccagatcaa gtgcccaagc ggacatcttc tgctcgacac acccttgaac acaggtgccg gcaaacacca cttcaccggg taatatctgc cgctctggca tcatacgaac gatacgttcc tccactgcct gcataagtgg agttcccggt agtctcatca gacgactttc cacctttcgg gttaaactgc taccccgtgt acgatccgcc ttgagcagat tctcagctcc gtccattgct ataagaagag acttcttgca acgcaccttc ttcaacaact gttgtattga ctgtactaca tgacgcgcca cttccttatg gcgacgctgc tcagttgtaa cgttctttgc gagacgtaac cccgtagcga cgagggcagt ggcgtccacc gcaacgtagt cacattcaat ttccttttta gttattaaca gctggaagcg atctgtaatt ttggactgtc ggtacgtcgt cgcagcaaag tgacgacatg actgagccaa tgccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMA LAQSCRHFAA TTYRQSKITD RFQLLITKKE IECDYVAVDA TALVATGLRL AKNVTTEQRR HKEVARHVVQ SIQQLLKKVR CKKSLLIAMD GAENLLKADR TRGSSLTRKV ESRLMRLPGT PLMQAVEERI VRMMPERQIL PGEVVFAGTC VQGCVEQKMS AWALDLASRD TFNGSSDSLN LIGASELYLN VLALSPFYNV SSVVQNNADL RHIRLQDILE WLELDKKAKE GESVTIAKMR TDILFLFIIA NGASATELNP IPAVGFHEIV DRYMKVLAEN GATPPSQSAT ASAPTASANV PAAPAFLFED SPGNTLQLNL RMLCRIMQLV ARKEAPARVD GLSEAYLEHA LQTHAMLCTG QAPQCTYLPA AGTGQLGSTA PSAVQLAGHL AALCASTAQR NARCTSLQPS LVTPEKGSCE EDSSATKLPP TPPTSAVAPV EDKPLTAAEY TILCQSLPAA VEGLIHQYVG VAPKPDVGKM ITTATTEEAY RLVREVLSYA NPLRPHKCLC LSPSYCWLQN EKTQLWRFEY VDIGVKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat tggctcagtc atgtcgtcac tttgctgcga cgacgtaccg acagtccaaa attacagatc gcttccagct gttaataact aaaaaggaaa ttgaatgtga ctacgttgcg gtggacgcca ctgccctcgt cgcaacgggg ttacgtctcg caaagaacgt tacaactgag cagcgtcgcc ataaggaagt ggcgcgtcat gtagtacagt caatacaaca gttgttgaag aaggtgcgtt gcaagaagtc tcttcttata gcaatggacg gagctgagaa tctgctcaag gcggatcgta cacggggtag cagtttaacc cgaaaggtgg aaagtcgtct gatgagacta ccgggaactc cacttatgca ggcagtggag gaacgtatcg ttcgtatgat gccagagcgg cagatattac ccggtgaagt ggtgtttgcc ggcacctgtg ttcaagggtg tgtcgagcag aagatgtccg cttgggcact tgatctggcg tcccgagaca cctttaacgg cagcagcgac tcactgaatt tgattggggc ctctgagttg taccttaatg tgttggcgct gtcgcccttc tataacgtat cgagtgtggt gcaaaataac gcagatttgc gacatatacg actgcaggat attttggagt ggctagagct agataagaag gcgaaggaag gggagagtgt cacaatagcc aagatgagaa cggatatttt attcctcttc attatcgcta acggcgcctc ggcaactgag ctgaatccca ttcctgctgt tggtttccat gaaattgttg ataggtacat gaaggttctc gcagaaaacg gtgctacgcc cccttctcaa agcgcgaccg cgagcgcccc aactgcttca gccaacgtcc cggccgcccc agcatttctt tttgaggata gtccgggcaa cacactccaa ctgaatcttc gcatgctctg tcgcatcatg cagttagtgg cgcgtaagga ggcgccagcc cgtgtggatg gtctgtcaga agcctacttg gagcacgcct tacaaacgca tgcaatgttg tgtactggac aggcacccca atgcacctac ttgccagcag cgggtacagg acaactaggc tcgacggcgc ctagcgccgt gcagctcgca ggtcatcttg ctgcattatg cgcatccaca gcacaacgca acgcacggtg tacttctctg cagccctcgc tggtaacacc agaaaaggga tcgtgtgagg aagacagttc ggcaacaaag ctgccgccga cacccccaac tagcgcggtc gcaccagttg aagacaagcc gctgacagca gctgaataca ccattctctg tcagtcgctt cccgcagctg tggaaggatt gattcatcag tacgtaggcg tagcgcctaa gccggatgtt gggaagatga ttacaacggc gaccacggag gaagcctacc gccttgttcg ggaagtgctt tcttatgcga accctcttcg accgcataag tgcttatgcc tttccccatc ttattgctgg ctccagaatg aaaagacgca actgtggcgc ttcgaatatg tcgatattgg tgtcaaaagt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrbrA.19505.a.B3.GE40775
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS LKLLTWNVMF DRYSGKPTPL GMPGIDWCSP KRYPVIAKII EEEDADVVGM QEVEPVFWEF LSKRPLIRQR YYFSCGHMSP AIAPWGVLML IHRRRLPVQS INYLNVPAWT NHVSLMPVVG LKMVHGTVHI AAAHLLAPYT KSHENARTSQ DTALRHHMTK TLGGGDVVTM GDFNDWPTNE FIMPHESQYV DCWPVLHPND PGKTMDETNT FCKLKVEEMF FGRSDKVFLR SRRLVPVEAH LVGTRSVNDE NNNADAPAYL FPSDHXXXSI TFS
Validated NT Sequence
atcttactca cttatgaaaa cgttatactt annnnntagt gatcggatgg gaacaaatat gcgggtgcgt cggcgttgtt gttctcatcg tttacactcc gtgttccgac gagatgagct tcgactggca caagccgcct gcttcgcaga aacaccttgt cggaacggcc aaaaaacatt tcctccactt tcagtttaca aaaggtgttt gtttcatcca tagtctttcc agggtcgttg gggtgcaaaa cgggccaaca gtccacgtac tgtgattcat gtggcataat aaattcattt gtaggccagt cgttgaaatc tcccatagtt accacatccc cgccacccaa cgtcttcgtc atgtgatggc gtaacgcggt atcctgggaa gtacgagcgt tctcgtggga cttcgtgtat ggcgcaagta agtgtgcggc agctatgtgt acagtaccgt gcaccatctt cagacccaca accggcatga gagaaacatg attagtccac gcagggacgt taagatagtt gatgctctgt actggaagac gccgacggtg tatcaacatc agcacccccc acggggcaat agctggactc atatgaccgc acgaaaagta gtacctttgg cgtataagtg gccgcttgga aaggaactcc caaaatacag gctccacctc ttgcatgcca acaacatccg catcctcctc ctctatgatc ttggcaatga cgggatacct cttgggggag caccaatcta taccaggcat acctaaagga gttggtttgc cgctgtaacg atcaaacatg acattccatg tcagcaattt taaagacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHSL KLLTWNVMFD RYSGKPTPLG MPGIDWCSPK RYPVIAKIIE EEDADVVGMQ EVEPVFWEFL SKRPLIRQRY YFSCGHMSPA IAPWGVLMLI HRRRLPVQSI NYLNVPAWTN HVSLMPVVGL KMVHGTVHIA AAHLLAPYTK SHENARTSQD TALRHHMTKT LGGGDVVTMG DFNDWPTNEF IMPHESQYVD CWPVLHPNNP GKTMDETNTF CKLKVEEMFF GRSDKVFLRS RRLVPVEAHL VGTRSVNDEN NNADAPAYLF PSDHYGVSIT FS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctttaa aattgctgac atggaatgtc atgtttgatc gttacagcgg caaaccaact cctttaggta tgcctggtat agattggtgc tcccccaaga ggtatcccgt cattgccaag atcatagagg aggaggatgc ggatgttgtt ggcatgcaag aggtggagcc tgtattttgg gagttccttt ccaagcggcc acttatacgc caaaggtact acttttcgtg cggtcatatg agtccagcta ttgccccgtg gggggtgctg atgttgatac accgtcggcg tcttccagta cagagcatca actatcttaa cgtccctgcg tggactaatc atgtttctct catgccggtt gtgggtctga agatggtgca cggtactgta cacatagctg ccgcacactt actcgcgcca tacacgaagt cccacgagaa cgctcgtact tcccaggata ccgcgttacg ccatcacatg acgaagacgt tgggtggcgg ggatgtggta actatgggag atttcaacga ctggcctaca aatgaattta ttatgccaca tgaatcacag tacgtggact gttggcccgt tttgcacccc aacaaccctg gaaagactat ggatgaaaca aacacctttt gtaaactgaa agtggaggaa atgttttttg gccgttccga caaggtgttt ctgcgaagca ggcggcttgt gccagtcgaa gctcatctcg tcggaacacg gagtgtaaac gatgagaaca acaacgccga cgcacccgca tatttgttcc catccgatca ctacggcgta agtataacgt tttcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat