TrbrA.19504.a

KREPB8

CENTER ID: TrbrA.19504.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TrbrA.19504.a.B1.GE40772 full length 1 368
TrbrA.19504.a.B2.GE40773 ordered domain 89 361
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: Tb927.8.5690
RefSeq: XP_847399.1
UniProt: Q57X13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHRGPPALLH TVGVATRSKS FAASTKLPGR LTPLPSTSST LQPTNTLLSS SAGIASLRQL VSASTLTSTS TSGKKRVGSL DAHCRLLSRG TSADSYMPMT SRSFDVFEGN NKQAWLDLKT GWCHLCQEPL GATMGVHIGD RDHTNLQYFL FLYAAHGRRW SSEEVLRSCL ATAPTVHNYA TRHTTWDHLH VMDDALRRAE LEALLFHLTH PPQQALSHVL QGRSPLGFWY SGERMWKLRI SRLVTQVFPP ISAGMMTNFT QKCWGRSNGE RMYDALRLQR IKAYYGWQPY ESKEKKAFFV RQLLWELLSV EVRDEVDELT KHMADLALRR MAFEMIFLQS MEYMHKVQYV YDLMGRPTVD ELAAMNLL
NT Sequence
atgcaccgcg gaccgccggc acttctgcat actgtaggcg tcgcgacacg gagcaaatca ttcgcagcga gtacgaaact accagggaga ttaacacctc tcccgtcgac atcttccacg ctccagccta ccaacacttt actgtcctct tcagcgggaa tagcctctct gcgccagctc gtcagcgcct ctacactaac cagcacttca acaagcggca agaaacgtgt cgggtcactg gatgctcact gtcgtctcct ctcaagagga acctcagccg atagttacat gccaatgacg tcacgctctt tcgatgtttt tgaaggtaac aacaagcagg catggctgga tctcaaaact ggctggtgcc atctctgcca agaaccacta ggtgcgacaa tgggtgtcca tattggcgac cgtgatcaca ctaatctaca gtattttctg tttctctacg cagctcatgg gcgtcgatgg tcaagtgagg aggttcttcg ttcctgtctc gccaccgctc cgacggtgca caactacgcg acaaggcaca caacgtggga ccacttgcat gtcatggacg acgcgctgcg tcgcgctgag ttggaggcgc ttcttttcca tctgacacac ccaccacaac aggccctgtc gcacgtgctt cagggtcgca gcccactggg attttggtac agtggagaac gtatgtggaa gttgcgtatc tcgcgcctcg tcacgcaggt gtttcccccc atctctgcgg ggatgatgac aaacttcaca cagaagtgct ggggtcgtag taacggcgaa aggatgtatg atgccttaag gttgcaacga ataaaggctt actatggctg gcaaccgtac gaaagtaagg agaagaaagc ctttttcgtg cggcaactgc tgtgggagct gctttccgtg gaggtacgtg acgaggtaga tgaattaaca aaacacatgg cggatcttgc gctacggcgt atggcgtttg agatgatctt cttgcagtca atggaataca tgcataaggt acaatatgtg tacgatttga tggggagacc cactgtggat gaacttgcag caatgaattt gctgtag
Details for TrbrA.19504.a.B1.GE40772
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 43kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMH RGPPALLHNV GVATRSKSFA ASTKLPGRLT PLPSTSSTLQ PTNTLLSSSA GIASLRQLVS ASTLTSTSTS GKKRVGSLDA HCRLLSRGTS ADSYMPMTSR SFDVFEGNNK QAWLDLKTGW CHLCQEPLGA TMGVHIGDRD HTNLQYFLFL YAAHGRRWSS EEVLRSCLAT APTVHNYATR HTTWDHLHVM DDALRRAELE ALLFHLTHPP QQALSHVLQG RSPLGFWYSG ERMWKLRISR LVTQVFPPIS AGMMTNFTQK CWGRSNGERM YDALRLQRIK AYYGWQPYES KEKKAFFVRQ LLWELLSVEV RDEVDELTKH MADLALRRMA FEMIFLQSME YMHKVQYVYD LMGRPTVD
Validated NT Sequence
ttcatccaca gtgggtctcc ccatcaaatc atacacatat tgtaccttat gcatgtattc cattgactgc aagaagatca tctcaaacgc catacgccgt agcgcaagat ccgccatgtg ttttgttaat tcatctacct cgtcacgtac ctccacggaa agcagctccc acagcagttg ccgcacgaaa aaggctttct tctccttact ttcgtacggt tgccagccat agtaagcctt tattcgttgc aaccttaagg catcatacat cctttcgccg ttactacgac cccagcactt ctgtgtgaag tttgtcatca tccccgcaga gatgggggga aacacctgcg taacgaggcg cgagatacgc aacttccaca tacgttctcc actgtaccaa aatccaagtg ggctgcgacc ctgaagcacg tgcgacaggg cctgttgtgg tgggtgcgtc agatggaaaa gaagcgcctc caactcagcg cgacgcagcg cgtcgtccat gacatgcaag tggtcccacg ttgtgtgcct tgtcgcgtag ttgtgcaccg tcggagcggt ggcgagacag gaacgaagaa cctcctcact tgaccatcga cgcccatgag ctgcgtagag aaacagaaaa tactgtagat tagtgtgatc acggtcgcca atatggacac ccattgtcgc acctagtggt tcttggcaga gatggcacca gccagtcttg agatccagcc atgcctgctt gttgttacct tcaaaaacat cgaaagagcg tgacgtcatt ggcatgtaac tatcggctga ggttcctctt gagaggagac gacagtgagc atccagtgac ccgacacgtt tcttgccgct tgttgaagtg ctggttagtg tagaggcgct gacgagctgg cgcagagaag ctattcccgc tgaagaggac agtaaagtgt tggtaggctg gagcgtggaa gatgtcgacg ggagaggtgt taatctccct ggtagtttcg tactcgctgc gaatgatttg ctccgtgtcg cgacacctac attatgcaga agtgccggcg gtccgcggtg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMH RGPPALLHTV GVATRSKSFA ASTKLPGRLT PLPSTSSTLQ PTNTLLSSSA GIASLRQLVS ASTLTSTSTS GKKRVGSLDA HCRLLSRGTS ADSYMPMTSR SFDVFEGNNK QAWLDLKTGW CHLCQEPLGA TMGVHIGDRD HTNLQYFLFL YAAHGRRWSS EEVLRSCLAT APTVHNYATR HTTWDHLHVM DDALRRAELE ALLFHLTHPP QQALSHVLQG RSPLGFWYSG ERMWKLRISR LVTQVFPPIS AGMMTNFTQK CWGRSNGERM YDALRLQRIK AYYGWQPYES KEKKAFFVRQ LLWELLSVEV RDEVDELTKH MADLALRRMA FEMIFLQSME YMHKVQYVYD LMGRPTVDEL AAMNLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcacc gcggaccgcc ggcacttctg catactgtag gcgtcgcgac acggagcaaa tcattcgcag cgagtacgaa actaccaggg agattaacac ctctcccgtc gacatcttcc acgctccagc ctaccaacac tttactgtcc tcttcagcgg gaatagcctc tctgcgccag ctcgtcagcg cctctacact aaccagcact tcaacaagcg gcaagaaacg tgtcgggtca ctggatgctc actgtcgtct cctctcaaga ggaacctcag ccgatagtta catgccaatg acgtcacgct ctttcgatgt ttttgaaggt aacaacaagc aggcatggct ggatctcaaa actggctggt gccatctctg ccaagaacca ctaggtgcga caatgggtgt ccatattggc gaccgtgatc acactaatct acagtatttt ctgtttctct acgcagctca tgggcgtcga tggtcaagtg aggaggttct tcgttcctgt ctcgccaccg ctccgacggt gcacaactac gcgacaaggc acacaacgtg ggaccacttg catgtcatgg acgacgcgct gcgtcgcgct gagttggagg cgcttctttt ccatctgaca cacccaccac aacaggccct gtcgcacgtg cttcagggtc gcagcccact gggattttgg tacagtggag aacgtatgtg gaagttgcgt atctcgcgcc tcgtcacgca ggtgtttccc cccatctctg cggggatgat gacaaacttc acacagaagt gctggggtcg tagtaacggc gaaaggatgt atgatgcctt aaggttgcaa cgaataaagg cttactatgg ctggcaaccg tacgaaagta aggagaagaa agcctttttc gtgcggcaac tgctgtggga gctgctttcc gtggaggtac gtgacgaggt agatgaatta acaaaacaca tggcggatct tgcgctacgg cgtatggcgt ttgagatgat cttcttgcag tcaatggaat acatgcataa ggtacaatat gtgtacgatt tgatggggag acccactgtg gatgaacttg cagcaatgaa tttgctgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for TrbrA.19504.a.B2.GE40773
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 33kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR GTSADSYMPM TSRSFDVFEG NNKQAWLDLK TGWCHLCQEP LGATMGVHIG DRDHTNLQYF LFLYAAHGRR WSSEEVLRSC LATAPTVHNY ATRHTTWDHL HVMDDALRRA ELEALLFHLT HPPQQALSHV LQGRSPLGFW YSGERMWKLR ISRLVTQVFP PISAGMMTNF TQKCWGRSNG ERMYDALRLQ RIKAYYGWQP YESKEKKAFF VRQLLWELLS VEVRDEVDEL TKHMADLALR RMAFEMIFLQ SMEYMHKVQY VYDLMGRPTV DE
Validated NT Sequence
tcacttattc atccacagtg ggtctcccca tcaaatcata cacatattgt accttatgca tgtattccat tgactgcaag aagatcatct caaacgccat acgccgtagc gcaagatccg ccatgtgttt tgttaattca tctacctcgt cacgtacctc cacggaaagc agctcccaca gcagttgccg cacgaaaaag gctttcttct ccttactttc gtacggttgc cagccatagt aagcctttat tcgttgcaac cttaaggcat catacatcct ttcgccgtta ctacgacccc agcacttctg tgtgaagttt gtcatcatcc ccgcagagat ggggggaaac acctgcgtaa cgaggcgcga gatacgcaac ttccacatac gttctccact gtaccaaaat ccaagtgggc tgcgaccctg aagcacgtgc gacagggcct gttgtggtgg gtgcgtcaga tggaaaagaa gcgcctccaa ctcagcgcga cgcagcgcgt cgtccatgac atgcaagtgg tcccacgttg tgtgccttgt cgcgtagttg tgcaccgtcg gagcggtggc gagacaggaa cgaagaacct cctcacttga ccatcgacgc ccatgagctg cgtagagaaa cagaaaatac tgtagattag tgtgatcacg gtcgccaata tggacaccca ttgtcgcacc tagtggttct tggcagagat ggcaccagcc agtcttgaga tccagccatg cctgcttgtt gttaccttca aaaacatcga aagagcgtga cgtcattggc atgtaactat cggctgaggt tcctctcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHRG TSADSYMPMT SRSFDVFEGN NKQAWLDLKT GWCHLCQEPL GATMGVHIGD RDHTNLQYFL FLYAAHGRRW SSEEVLRSCL ATAPTVHNYA TRHTTWDHLH VMDDALRRAE LEALLFHLTH PPQQALSHVL QGRSPLGFWY SGERMWKLRI SRLVTQVFPP ISAGMMTNFT QKCWGRSNGE RMYDALRLQR IKAYYGWQPY ESKEKKAFFV RQLLWELLSV EVRDEVDELT KHMADLALRR MAFEMIFLQS MEYMHKVQYV YDLMGRPTVD E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagaggaa cctcagccga tagttacatg ccaatgacgt cacgctcttt cgatgttttt gaaggtaaca acaagcaggc atggctggat ctcaaaactg gctggtgcca tctctgccaa gaaccactag gtgcgacaat gggtgtccat attggcgacc gtgatcacac taatctacag tattttctgt ttctctacgc agctcatggg cgtcgatggt caagtgagga ggttcttcgt tcctgtctcg ccaccgctcc gacggtgcac aactacgcga caaggcacac aacgtgggac cacttgcatg tcatggacga cgcgctgcgt cgcgctgagt tggaggcgct tcttttccat ctgacacacc caccacaaca ggccctgtcg cacgtgcttc agggtcgcag cccactggga ttttggtaca gtggagaacg tatgtggaag ttgcgtatct cgcgcctcgt cacgcaggtg tttcccccca tctctgcggg gatgatgaca aacttcacac agaagtgctg gggtcgtagt aacggcgaaa ggatgtatga tgccttaagg ttgcaacgaa taaaggctta ctatggctgg caaccgtacg aaagtaagga gaagaaagcc tttttcgtgc ggcaactgct gtgggagctg ctttccgtgg aggtacgtga cgaggtagat gaattaacaa aacacatggc ggatcttgcg ctacggcgta tggcgtttga gatgatcttc ttgcagtcaa tggaatacat gcataaggta caatatgtgt acgatttgat ggggagaccc actgtggatg aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat