SSGCID: Target Page

TrbrA.19503.a

KREPB7

CENTER ID:	TrbrA.19503.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19503.a.B2.GE40771	RNase III-like catalytic-like domain		88	411

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	Tb927.9.5630
RefSeq:	XP_826958.1
UniProt:	Q38EZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFQKALRRLQ QQASQNPPVG SANIPSPYAT EGGHVPIWSI VNGFVQKRNW VFRNPEWCDL CKEPVALWVN HHGRKDHALM DMHYTQMMEY PRRWNPEEVL VAFFDELQLP VETYQRSYTC YERAHRNELY SMLVELEEAK MLYFGEPRNT YLHRMQGGLR GMDHQGALVL HRYILGPFMR LYPNAHIQDF SNLVDFVTCA YNMETVYDLC GFYTLDKVAL KADYKPSSPA ALGLGGIAVH SSPSHGFVQN AAESQQQSRR ASSTASTGSR SAADMDEEAF SRKAVFVRQL LGQLRWLTLP CEEHPAGCKF PQHLMLLGEI CLKYLVVEII AARLCEYMVR VEPVWRDHGY ERVKLNVDKI MKQGRDILPE PIKYFYRPMS PNMDDLYSTK VGVLDEALEK AAVTQSLSKG V

NT Sequence

atgtttcaaa aagcacttcg tcgactgcag caacaggcat cacagaatcc accagttggt tccgccaata ttccttcacc ctacgcaacg gaaggtgggc acgtccccat ttggtccatc gtaaacggct tcgtgcagaa acgtaattgg gtgttccgta accctgagtg gtgtgacctt tgcaaagaac ctgtggcatt gtgggtgaac catcacggtc gtaaggacca cgccttaatg gacatgcact atacacaaat gatggaatat ccgaggcggt ggaaccccga ggaagttctc gtggcatttt ttgacgaact acaactcccc gtagaaacgt atcagcgtag ctatacatgt tacgaacgtg cccataggaa tgagctttac tccatgcttg tggagttgga ggaggccaaa atgctttact ttggggagcc gcgaaacact taccttcacc gaatgcaagg tggtttgcgt ggtatggacc atcagggtgc acttgtgctg caccgctaca tcctaggccc cttcatgcgg ctatacccca atgctcacat acaggacttc tcaaacctcg tggactttgt aacctgcgcg tacaatatgg aaactgttta cgatttgtgt ggtttctaca cacttgataa agtagcatta aaggccgact ataaaccgag ctcaccggca gctttggggt taggaggaat cgccgtacac tcctcccctt cgcatggatt tgttcagaac gcggcagagt cccagcagca gtcgaggcga gcgagttcaa ccgcttcaac ggggtctcgt agtgctgcgg acatggatga agaggctttt tcacgtaagg ctgtttttgt taggcagttg ttaggtcaac tgcgatggtt gacacttcca tgtgaagagc atccggcagg ttgtaaattc ccccaacacc tgatgctctt gggtgagatt tgcctcaagt atcttgtggt ggagataata gcggcacgcc tgtgtgaata tatggtgcga gtggagccgg tgtggcgtga ccatggttac gagcgggtca aactcaacgt tgataaaatc atgaaacagg gaagggatat attacccgag ccaataaagt acttttaccg accaatgtca cccaatatgg atgacttgta cagtaccaaa gtaggcgtgt tggatgaggc actggaaaag gctgcggtta ctcaatcact ctctaaaggt gtgtag

Details for TrbrA.19503.a.B2.GE40771

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	38kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHME YPRRWNPEEV LVAFFDELQL PVETYQRSYT CYERAHRNEL YSMLVELEEA KMLYFGEPRN TYLHRMQGGL RGMDHQGALV LHRYILGPFM RLYPNAHIQD FSNLVDFVTC AYNMETVYDL CGFYTLDKVA LKADYKPSSP AALGLGGIAV HSSPSHGFVQ NAAESQQQSR RASSTASTGS RSAADMDEEA FSRKAVFVRQ LLGQLRWLTL PCEEHPAGCK FPQHLMLLGE ICLKYLVVEI IAARLCEYMV RVEPVWRDHG YERVKLNVDK IMKQGRDILP EPIKYFYRPM SPNMDDLYST KVGVLDEALE KAAVTQSLSK

Validated NT Sequence

cctttagaga gtgattgagt aaccgcagcc ttttccagtg cctcatccaa cacgcctact ttggtactgt acaagtcatc catattgggt gacattggtc ggtaaaagta ctttattggc tcgggtaata tatcccttcc ctgtttcatg atcttatcaa cgttgagttt aacccgctcg taaccatggt cacgccacac cggctccact cgcaccatat attcacacag gcgtgccgct attatctcca ccacaagata cttgaggcaa atctcaccca agagcatcag gtgttggggg aatttacaac ctgccggatg ctcttcacat ggaagtgtca accatcgcag ttgacctaac aactgtctaa caaaaacagc cttacgtgaa aaagcctctt catccatgtc cgcagcacta cgagaccccg ttgaagcggt tgaacttgct cgcctcgact gctgctggga ctctgccgcg ttctgaacaa atccatgcga aggggaggag tgtacggcga ttcctcctaa ccccaaagct gccggtgagc tcggtttata gtcggccttt aatgctactt tatcaagtgt gtagaaaccg cacaaatcgt aaacagtttc catattgtac gcgcaggtta caaagtccac gaggtttgag aagtcctgta tgtgagcatt ggggtatagc cgcatgaagg ggcctaggat gtagcggtgc agcacaagtg caccctgatg gtccatacca cgcaaaccac cttgcattcg gtgaaggtaa gtgtttcgcg gctccccaaa gtaaagcatt ttggcctcct ccaactccac aagcatggag taaagctcat tcctatgggc acgttcgtaa catgtatagc tacgctgata cgtttctacg gggagttgta gttcgtcaaa aaatgccacg agaacttcct cggggttcca ccgcctcgga tattccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat atccgaggcg gtggaacccc gaggaagttc tcgtggcatt ttttgacgaa ctacaactcc ccgtagaaac gtatcagcgt agctatacat gttacgaacg tgcccatagg aatgagcttt actccatgct tgtggagttg gaggaggcca aaatgcttta ctttggggag ccgcgaaaca cttaccttca ccgaatgcaa ggtggtttgc gtggtatgga ccatcagggt gcacttgtgc tgcaccgcta catcctaggc cccttcatgc ggctataccc caatgctcac atacaggact tctcaaacct cgtggacttt gtaacctgcg cgtacaatat ggaaactgtt tacgatttgt gtggtttcta cacacttgat aaagtagcat taaaggccga ctataaaccg agctcaccgg cagctttggg gttaggagga atcgccgtac actcctcccc ttcgcatgga tttgttcaga acgcggcaga gtcccagcag cagtcgaggc gagcgagttc aaccgcttca acggggtctc gtagtgctgc ggacatggat gaagaggctt tttcacgtaa ggctgttttt gttaggcagt tgttaggtca actgcgatgg ttgacacttc catgtgaaga gcatccggca ggttgtaaat tcccccaaca cctgatgctc ttgggtgaga tttgcctcaa gtatcttgtg gtggagataa tagcggcacg cctgtgtgaa tatatggtgc gagtggagcc ggtgtggcgt gaccatggtt acgagcgggt caaactcaac gttgataaaa tcatgaaaca gggaagggat atattacccg agccaataaa gtacttttac cgaccaatgt cacccaatat ggatgacttg tacagtacca aagtaggcgt gttggatgag gcactggaaa aggctgcggt tactcaatca ctctctaaag gtgtgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat