TrbrA.19502.a

KREPA1

CENTER ID: TrbrA.19502.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.19502.a.B2.GE40770 C2H2 zinc finger and OB fold 488 762
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.19502.a.B2.PS38188 C2H2 zinc finger and OB fold 488 762

External Resources

RESOURCE REFERENCE ID
EuPathDB: Tb927.2.2470
RefSeq: XP_951549.1
UniProt: Q586L9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRLTRRSGR LSGKGNGGSC LQMSPTHVGA VVTWALNRLM PLHTRTIPLR CSLPTPESGT TEPRELCFYE TFELTEEDVH YLLLHEAHVK HGVLLNVPPQ LAPNGTPPEV PEVIMPAAQL ERMGGMKLAY EPTHLPPPLH TTGARQLVLD ESFYTTPTKE KKATTTAVSH VSESTAASGG RGGASATAAG TALPPRLPPD PTMKFHCSAC GKAFRLKFSA DHHVKLNHGS DPKAAVVDGP GEGELLGGAV TITTAKVAKH SSSAASGTAS RAGDSATLDV KQQPDPQKEL SAPGISAVKI PYSKAVLSLP DDELVDELLI DVWDAVAAQR DDVPKSNSAN IFLPFASVVT GTADRRKEME AVARPTARAT PEGAAPGIKR PGAMAGGAVA VGKGRSGGQI LPIRELIKKY PNPFGDSPNA AVQDLENEPL NPFLPEEELA AQLQVACEED TVVTPSACTT DVSTGSVIGK KGSLEKLKEK LRGTRPSMAA SAAKRRFTCP ICVEKQQTLQ QQQSENVGSG FCTDIPSFRL LDALLDHVES VHGEELTEDQ LRELYAKQRQ STLYPQKSST GDGAGSRETP DDSEKKEGSV GNTNMDELKS LPEEVRRVVP PAPVEQDALA VHIRAGSNAL MIGRIADVQH GFLGAMTVTQ YVLEVDGDER INSKGVTTPA SACTPDPAST KAVEAKGEEG EVVEPEKEFI VIRCMGDNFP ASLLKDQVKL GSRVLVQGTL RMNRHVDDVS KRLHAYPFIQ VVPPLGYVKV VG
NT Sequence
atgagacgcc tcacccgccg cagtggccgt ctcagcggca agggcaacgg cggttcatgc ctccaaatgt ccccaacaca tgtgggggcg gttgtcacgt gggcgttaaa ccgtttgatg ccgctgcaca cccgcacaat tccactccgg tgttcgcttc cgacacctga aagtggcacc actgagccac gcgagctctg cttttacgaa accttcgaac ttactgagga ggatgtacac tatttattgc ttcatgaagc acatgtgaag catggagtac ttttaaatgt tccgccacag ttagcaccaa atggaacgcc tcccgaggtg ccggaggtga ttatgccagc agcacagttg gagcgcatgg gcgggatgaa attagcttac gaacccacac atctgccgcc accacttcac acgacaggtg cacggcagtt agttctagac gaaagtttct acacgacacc aactaaggaa aagaaggcta caaccaccgc tgtttcacac gtgagtgaat cgacggcggc gtcgggcgga agaggcggtg caagtgccac ggcagctggt accgctttac cgcctcgctt gcctccggac ccaacaatga agtttcactg cagcgcctgc ggcaaggcct ttcgcctgaa gttttctgca gaccaccatg tgaagttaaa ccatggatca gatccaaaag cggcggtggt tgatggtccc ggagagggcg aacttctcgg gggagcagta acgataacta cagcaaaggt agcaaagcat tcatcgtctg ctgcaagcgg aactgcctca cgggcgggtg attctgcaac tctggatgta aagcaacaac cggatccgca aaaggagttg tctgcccctg gcatcagcgc cgtgaagata ccgtacagta aagctgtgct ctcgctcccg gatgatgagc ttgtggacga gcttcttatc gatgtgtggg atgcggttgc ggctcaacgt gatgatgtac cgaagtcaaa tagtgcaaac atcttcctgc cgtttgcctc cgttgtcacg ggcactgccg acagacgcaa agagatggaa gcggttgctc ggcccaccgc ccgggccaca cctgagggag ccgcgccagg aattaaaagg cccggagcga tggcgggcgg ggccgtggcg gttggaaagg gccgcagtgg cggacagata ttacccatcc gagaactgat aaagaaatat ccaaatccct tcggtgactc ccccaacgcg gcggtgcagg atctggaaaa tgagccgctg aatccgtttt tgcctgaaga ggagttggct gcgcagttgc aggtagcgtg tgaggaagat actgttgtaa ctccctccgc atgcaccaca gatgtttcca ctggctccgt cattggaaaa aagggttccc tggagaagct aaaggaaaag cttcgcggca cgcgaccaag tatggcagct tccgcagcga aacggcgctt cacatgccca atctgcgtcg aaaagcaaca aacacttcaa caacagcaaa gtgaaaatgt cggcagcggt ttctgcacgg atattccatc ttttaggcta cttgatgctc ttctcgacca cgtggagtcg gtgcacggag aggagttgac ggaggatcaa ctgcgcgagc tatacgccaa gcaaagacag tctacacttt atccacagaa gtcctctacc ggtgatggag ccgggagccg cgaaactcct gatgattcgg aaaagaaaga aggtagcgtc ggcaatacca atatggatga gctgaaatct cttccagaag aagtcaggcg ggtcgtacca cctgcccctg tcgagcaaga cgcattggca gttcacatcc gcgccgggag taatgcgcta atgattggtc gcatagcgga tgtgcaacac ggtttccttg gtgccatgac cgttacgcag tatgtccttg aggttgacgg tgatgagaga ataaattcaa agggagtaac cacaccggct tcagcgtgca caccggaccc agcctccaca aaggcagttg aagcaaaggg tgaagaaggg gaagtggtgg aaccggagaa ggagtttatt gtcattcgat gtatgggtga caacttcccc gcctcattgt tgaaagatca ggtgaaactc ggcagccgtg tgctggtgca aggcacgttg cgtatgaata ggcacgttga cgacgtctca aaacggttgc atgcgtaccc cttcatccag gtggttccac ccctcggcta tgtgaaagtt gtagggtaa
Details for TrbrA.19502.a.B2.GE40770
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA ASAAKRRFTC PICVEKQQTL QQQQSENVGS GFCTDIPSFR LLDALLDHVE SVHGEELTED QLRELYAKQR QSTLYPQKSS TGDGAGSRET PDDSEKKEGS VGNTSMDELK SLPEEVRRVV PPAPVEQDAL AVHIRAGSNA LMIGRIADVQ HGFLGAMTVT QYVLEVDGDE RINSKGVTTP ASACTPDPAS TKAVEAKGEE GEVVEPEKEF IVIRCMGDNF PASLLKDQVK LGSRVLVQGT LRMNRHVDDV SKRLHAYPFI QVVPPLXXVK VVG
Validated NT Sequence
atcttactca cttaccctac aactttcaca tnnncgaggg gtggaaccac ctggatgaag gggtacgcat gcaaccgttt tgagacgtcg tcaacgtgcc tattcatacg caacgtgcct tgcaccagca cacggctgcc gagtttcacc tgatctttca acaatgaggc ggggaagttg tcacccatac atcgaatgac aataaactcc ttctccggtt ccaccacttc cccttcttca ccctttgctt caactgcctt tgtggaggct gggtccggtg tgcacgctga agccggtgtg gttactccct ttgaatttat tctctcatca ccgtcaacct caaggacata ctgcgtaacg gtcatggcac caaggaaacc gtgttgcaca tccgctatgc gaccaatcat tagcgcatta ctcccggcgc ggatgtgaac tgccaatgcg tcttgctcga caggggcagg tggtacgacc cgcctgactt cttctggaag agatttcagc tcatccatac tggtattgcc gacgctacct tctttctttt ccgaatcatc aggagtttcg cggctcccgg ctccatcacc ggtagaggac ttctgtggat aaagtgtaga ctgtctttgc ttggcgtata gctcgcgcag ttgatcctcc gtcaactcct ctccgtgcac cgactccacg tggtcgagaa gagcatcaag tagcctaaaa gatggaatat ccgtgcagaa accgctgccg acattttcac tttgctgttg ttgaagtgtt tgttgctttt cgacgcagat tgggcatgtg aagcgccgtt tcgctgcgga agctgccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMA ASAAKRRFTC PICVEKQQTL QQQQSENVGS GFCTDIPSFR LLDALLDHVE SVHGEELTED QLRELYAKQR QSTLYPQKSS TGDGAGSRET PDDSEKKEGS VGNTNMDELK SLPEEVRRVV PPAPVEQDAL AVHIRAGSNA LMIGRIADVQ HGFLGAMTVT QYVLEVDGDE RINSKGVTTP ASACTPDPAS TKAVEAKGEE GEVVEPEKEF IVIRCMGDNF PASLLKDQVK LGSRVLVQGT LRMNRHVDDV SKRLHAYPFI QVVPPLGYVK VVG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag cttccgcagc gaaacggcgc ttcacatgcc caatctgcgt cgaaaagcaa caaacacttc aacaacagca aagtgaaaat gtcggcagcg gtttctgcac ggatattcca tcttttaggc tacttgatgc tcttctcgac cacgtggagt cggtgcacgg agaggagttg acggaggatc aactgcgcga gctatacgcc aagcaaagac agtctacact ttatccacag aagtcctcta ccggtgatgg agccgggagc cgcgaaactc ctgatgattc ggaaaagaaa gaaggtagcg tcggcaatac caatatggat gagctgaaat ctcttccaga agaagtcagg cgggtcgtac cacctgcccc tgtcgagcaa gacgcattgg cagttcacat ccgcgccggg agtaatgcgc taatgattgg tcgcatagcg gatgtgcaac acggtttcct tggtgccatg accgttacgc agtatgtcct tgaggttgac ggtgatgaga gaataaattc aaagggagta accacaccgg cttcagcgtg cacaccggac ccagcctcca caaaggcagt tgaagcaaag ggtgaagaag gggaagtggt ggaaccggag aaggagttta ttgtcattcg atgtatgggt gacaacttcc ccgcctcatt gttgaaagat caggtgaaac tcggcagccg tgtgctggtg caaggcacgt tgcgtatgaa taggcacgtt gacgacgtct caaaacggtt gcatgcgtac cccttcatcc aggtggttcc acccctcggc tatgtgaaag ttgtagggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for TrbrA.19502.a.B2.PS38188
PURIFICATION DATe: 2/1/2017
CONCENTRATION: 37.3mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA ASAAKRRFTC PICVEKQQTL QQQQSENVGS GFCTDIPSFR LLDALLDHVE SVHGEELTED QLRELYAKQR QSTLYPQKSS TGDGAGSRET PDDSEKKEGS VGNTSMDELK SLPEEVRRVV PPAPVEQDAL AVHIRAGSNA LMIGRIADVQ HGFLGAMTVT QYVLEVDGDE RINSKGVTTP ASACTPDPAS TKAVEAKGEE GEVVEPEKEF IVIRCMGDNF PASLLKDQVK LGSRVLVQGT LRMNRHVDDV SKRLHAYPFI QVVPPLXXVK VVG
Validated NT Sequence
atcttactca cttaccctac aactttcaca tnnncgaggg gtggaaccac ctggatgaag gggtacgcat gcaaccgttt tgagacgtcg tcaacgtgcc tattcatacg caacgtgcct tgcaccagca cacggctgcc gagtttcacc tgatctttca acaatgaggc ggggaagttg tcacccatac atcgaatgac aataaactcc ttctccggtt ccaccacttc cccttcttca ccctttgctt caactgcctt tgtggaggct gggtccggtg tgcacgctga agccggtgtg gttactccct ttgaatttat tctctcatca ccgtcaacct caaggacata ctgcgtaacg gtcatggcac caaggaaacc gtgttgcaca tccgctatgc gaccaatcat tagcgcatta ctcccggcgc ggatgtgaac tgccaatgcg tcttgctcga caggggcagg tggtacgacc cgcctgactt cttctggaag agatttcagc tcatccatac tggtattgcc gacgctacct tctttctttt ccgaatcatc aggagtttcg cggctcccgg ctccatcacc ggtagaggac ttctgtggat aaagtgtaga ctgtctttgc ttggcgtata gctcgcgcag ttgatcctcc gtcaactcct ctccgtgcac cgactccacg tggtcgagaa gagcatcaag tagcctaaaa gatggaatat ccgtgcagaa accgctgccg acattttcac tttgctgttg ttgaagtgtt tgttgctttt cgacgcagat tgggcatgtg aagcgccgtt tcgctgcgga agctgccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMA ASAAKRRFTC PICVEKQQTL QQQQSENVGS GFCTDIPSFR LLDALLDHVE SVHGEELTED QLRELYAKQR QSTLYPQKSS TGDGAGSRET PDDSEKKEGS VGNTNMDELK SLPEEVRRVV PPAPVEQDAL AVHIRAGSNA LMIGRIADVQ HGFLGAMTVT QYVLEVDGDE RINSKGVTTP ASACTPDPAS TKAVEAKGEE GEVVEPEKEF IVIRCMGDNF PASLLKDQVK LGSRVLVQGT LRMNRHVDDV SKRLHAYPFI QVVPPLGYVK VVG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG CTTCCGCAGC GAAACGGCGC TTCACATGCC CAATCTGCGT CGAAAAGCAA CAAACACTTC AACAACAGCA AAGTGAAAAT GTCGGCAGCG GTTTCTGCAC GGATATTCCA TCTTTTAGGC TACTTGATGC TCTTCTCGAC CACGTGGAGT CGGTGCACGG AGAGGAGTTG ACGGAGGATC AACTGCGCGA GCTATACGCC AAGCAAAGAC AGTCTACACT TTATCCACAG AAGTCCTCTA CCGGTGATGG AGCCGGGAGC CGCGAAACTC CTGATGATTC GGAAAAGAAA GAAGGTAGCG TCGGCAATAC CAATATGGAT GAGCTGAAAT CTCTTCCAGA AGAAGTCAGG CGGGTCGTAC CACCTGCCCC TGTCGAGCAA GACGCATTGG CAGTTCACAT CCGCGCCGGG AGTAATGCGC TAATGATTGG TCGCATAGCG GATGTGCAAC ACGGTTTCCT TGGTGCCATG ACCGTTACGC AGTATGTCCT TGAGGTTGAC GGTGATGAGA GAATAAATTC AAAGGGAGTA ACCACACCGG CTTCAGCGTG CACACCGGAC CCAGCCTCCA CAAAGGCAGT TGAAGCAAAG GGTGAAGAAG GGGAAGTGGT GGAACCGGAG AAGGAGTTTA TTGTCATTCG ATGTATGGGT GACAACTTCC CCGCCTCATT GTTGAAAGAT CAGGTGAAAC TCGGCAGCCG TGTGCTGGTG CAAGGCACGT TGCGTATGAA TAGGCACGTT GACGACGTCT CAAAACGGTT GCATGCGTAC CCCTTCATCC AGGTGGTTCC ACCCCTCGGC TATGTGAAAG TTGTAGGGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT