TrbrA.19501.a

JBP3 (glucosyl transferase interacting protein 2) (GTIP2)

CENTER ID:	TrbrA.19501.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19501.a.B5.GE41048	N-extended J-binding domain		48	243

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19501.a.B5.PS38215	N-extended J-binding domain		48	243

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79393

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.10.4800
UniProt:	Q38BC1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSSGYDTSY KSSLSSAPSS VGSRTSRQIS EGATSVFSGE TSSVSGHDPV FRNVKRNPNL QFLKPDDSVI HVSPSARNVV RYVASNYDSF PDGHYRVVDY FRVAVEMFLQ QVRLEYEKQA VQAVKNGVKK PRYTWGNKSG LAISYATSCD MLKLLYDVIS EENRPKWDEV VKQFIYFLIS ESGIPGGVFS EQTYHTKFLD WRKGGTRYQN LSMASNVRFP SASHRRQGVE NYLRSGSWLS MKTTEVTVLA NTDGTSRGES MGGVTDSTPS CSNSHASSMC YPPATRIQQR INMEHPVVPA MAKQVDDFSP RRTRWREKLT QIMGSPSARG LGEDRSNMVL TFVRLCQVCD SAEQAESLTD IIVESDESIQ RSFEGNGGIA VLRRFVGEPK WGCQFKFLFG LVEKLLRMRL MSWGTESRQS WLRDLNGRGR RNLDWLRNIE VIPKGEREKW TNLVRELEAR YVLMPSHRDL EQPARVKRPP TGREGLEQKV ASSDGHSVKP QLCVSNSRCR RLPTEFLTLP DPEAEGDVNN FMDRKAKYCE DGQAEWRKAL RKAAGVDLGG VSVPIWYDPY QLLDTSQCSS A

NT Sequence

atgggttcgt cggggtatga cacttcatat aagagttctc tgagtagcgc gccaagctca gtcggttccc gcactagcag acagatatca gagggtgcaa cgagcgtttt ctctggtgaa acttcctcag tttccggtca tgacccagtg ttccgcaatg ttaagcgaaa cccaaatcta cagttcctaa aacccgacga tagtgttatt catgtttccc cttctgcgcg taacgtagtc cggtatgttg cttccaacta tgatagtttt ccagacggcc attacagagt tgttgactat tttcgagtgg ctgttgagat gttccttcaa caggtacgtt tggagtatga aaagcaggct gttcaggcgg tgaagaatgg tgtaaaaaaa ccccggtata catggggaaa caagagcgga ctggcgatct cttacgcgac tagctgcgac atgttaaagt tactctacga tgtcatatct gaagaaaacc gccccaagtg ggacgaggtg gtcaaacagt ttatttattt cctaatctcc gaatcgggta tacctggggg tgtcttttcg gagcaaacat atcatacaaa gttccttgac tggcggaaag gggggacacg ataccaaaac cttagcatgg cgtcgaatgt gcgcttccca tccgcttccc acaggcgcca aggtgtagaa aactacctcc ggagtggatc gtggctaagt atgaaaacaa cggaggttac cgtactcgct aacacggacg gtacatccag aggcgagagc atgggagggg ttacggattc cactccttcc tgctcgaact cacacgccag tagcatgtgt tatccaccag ccactagaat ccagcagagg ataaacatgg aacaccctgt cgtacccgct atggcaaaac aggtagacga cttttcacct agacgaacac gctggaggga aaaattgaca caaatcatgg ggagcccttc agcgagggga ttgggagagg atagatcaaa catggtactg acttttgttc gtctatgtca ggtatgtgac tccgccgagc aggcagaaag tttaacggac atcattgtgg aatctgacga gagcattcaa cgttcgtttg aaggtaacgg tggaatagct gtcctgcgac gtttcgtggg tgaacccaag tggggatgcc aatttaaatt tctttttggt cttgtggaga agctgctccg gatgcgactt atgtcatggg gtaccgagag tcgccagtca tggctgcgtg acctgaacgg ccgaggccgg cggaacctgg actggcttcg gaacatcgaa gtaattccaa agggagagcg tgagaagtgg acaaacttag tgagggagct cgaagctcgg tacgtgctca tgccttcgca tcgggatcta gagcaacctg cccgtgtgaa gcgtccgcca acgggccgcg aggggctcga gcaaaaagta gcttccagcg atggacactc ggtcaagccc cagctctgtg tttccaattc ccgctgcagg cggcttccta cggagtttct tacgctaccg gatcctgaag cggaggggga cgtaaacaac ttcatggacc gcaaggcgaa gtattgcgag gacggtcagg cggagtggcg taaggctctt cgtaaagctg cgggtgtgga cctcgggggt gtgtcggttc cgatttggta cgatccgtat cagttattgg atacaagtca atgttcttct gcgtag

Details for TrbrA.19501.a.B5.GE41048

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD PVFRNVKRNP NLQFLKPDDS VIHVSPSARN VVRYIASNYD SFPDGHYRVV DYFRVAVEMF LQQVRLEYEK QAVQAVKNGV KKPRYTWGNK SGLAISYATS CDMLKLLYDV ISEENRPKWD EVVKQFIYFL ISESGIPGGV FSEQTYHTKF LDWRKGGTRY QNLSIASNVR FPSASHRRQG VENYLRSGSW LSMKT

Validated NT Sequence

atcttactca cttatgtttt catacttagc cacgatccac tccggaggta gttttctaca ccttggcgcc tgtgggaagc ggatgggaag cgcacattcg acgctatgct aaggttttgg tatcgtgtcc cccctttccg ccagtcaagg aactttgtat gatatgtttg ctccgaaaag acaccccctg gtatacccga ttcggagatt aggaaataaa taaactgttt gaccacctcg tcccacttgg ggcggttttc ttcagatatg acatcgtaga gtaactttaa catgtcgcag ctagtcgcgt aagagatcgc cagtccgctc ttgtttcccc atgtataccg gggttttttt acaccattct tcaccgcctg aacagcctgc ttttcatact ccaaacgtac ctgttgaagg aacatctcaa cagccactcg aaagtagtca acaactctgt aatggccgtc tggaaaacta tcatagttgg aagcaatata ccggactacg ttacgcgcag aaggggaaac atgaataaca ctatcgtcgg gttttaggaa ctgtagattt gggtttcgct taacattgcg gaacactggg tccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHDP VFRNVKRNPN LQFLKPDDSV IHVSPSARNV VRYVASNYDS FPDGHYRVVD YFRVAVEMFL QQVRLEYEKQ AVQAVKNGVK KPRYTWGNKS GLAISYATSC DMLKLLYDVI SEENRPKWDE VVKQFIYFLI SESGIPGGVF SEQTYHTKFL DWRKGGTRYQ NLSMASNVRF PSASHRRQGV ENYLRSGSWL SMKT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacccag tgttccgcaa tgttaagcga aacccaaatc tacagttcct aaaacccgac gatagtgtta ttcatgtttc cccttctgcg cgtaacgtag tccggtatgt tgcttccaac tatgatagtt ttccagacgg ccattacaga gttgttgact attttcgagt ggctgttgag atgttccttc aacaggtacg tttggagtat gaaaagcagg ctgttcaggc ggtgaagaat ggtgtaaaaa aaccccggta tacatgggga aacaagagcg gactggcgat ctcttacgcg actagctgcg acatgttaaa gttactctac gatgtcatat ctgaagaaaa ccgccccaag tgggacgagg tggtcaaaca gtttatttat ttcctaatct ccgaatcggg tatacctggg ggtgtctttt cggagcaaac atatcataca aagttccttg actggcggaa aggggggaca cgataccaaa accttagcat ggcgtcgaat gtgcgcttcc catccgcttc ccacaggcgc caaggtgtag aaaactacct ccggagtgga tcgtggctaa gtatgaaaac atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for TrbrA.19501.a.B5.PS38215

PURIFICATION DATe:	3/1/2017
CONCENTRATION:	34.6mg/ml
OBSERVED MW:	24kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC buffer: 20mM HEPES pH 7.0, 300mM NaCl, 55 glycerol, 1mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGACCCAG TGTTCCGCAA TGTTAAGCGA AACCCAAATC TACAGTTCCT AAAACCCGAC GATAGTGTTA TTCATGTTTC CCCTTCTGCG CGTAACGTAG TCCGGTATGT TGCTTCCAAC TATGATAGTT TTCCAGACGG CCATTACAGA GTTGTTGACT ATTTTCGAGT GGCTGTTGAG ATGTTCCTTC AACAGGTACG TTTGGAGTAT GAAAAGCAGG CTGTTCAGGC GGTGAAGAAT GGTGTAAAAA AACCCCGGTA TACATGGGGA AACAAGAGCG GACTGGCGAT CTCTTACGCG ACTAGCTGCG ACATGTTAAA GTTACTCTAC GATGTCATAT CTGAAGAAAA CCGCCCCAAG TGGGACGAGG TGGTCAAACA GTTTATTTAT TTCCTAATCT CCGAATCGGG TATACCTGGG GGTGTCTTTT CGGAGCAAAC ATATCATACA AAGTTCCTTG ACTGGCGGAA AGGGGGGACA CGATACCAAA ACCTTAGCAT GGCGTCGAAT GTGCGCTTCC CATCCGCTTC CCACAGGCGC CAAGGTGTAG AAAACTACCT CCGGAGTGGA TCGTGGCTAA GTATGAAAAC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT