TrbrA.19310.a

Phosphatidylinositol 4-phosphate 5-kinase alpha, putative (Pi4Ka)

CENTER ID: TrbrA.19310.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.19310.a.B1.GE42090 full length 1 481
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tb927.4.1620
UniProt: Q581R9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPFNREILAA ISFESGPLSS AEIAARRTPT EEPPKNLEVK SCDKRDEPRQ NEATINLIRC MKWALGQSIT SISHPAKQRP LNPKRDFSIK TKINLDVGVE KPKPGFCCST AAATGVREQR NHHAPFYDAH GDSEEASHLD ESDVESHDEV PNVPLAFVDY SPMCYRHIRE FFNIDHRAYR DVLLRSRWHS TPTPGKSAAQ LFFCGRDWVI KTMTHEESKF LRRILHRYYF FVLDHPYTLL PHFVGHHRLE INGEKMYIII MQNVFVTGNT IHEKYDLKGS TVGRFATEAE KKKQTCTQKD LDINRPLHVG ATRRNLLIEQ MKMDCGFLNQ CNIMDYSFLV GIHVLPMYEA HRPAPSTGSR EYLGGAAALA TSALEPSYLL GMGETMQPMA LNGHPTAAAD SAPTDGRCFK ADQGGMMSAD HPGGRREIYY IGIIDILQEY NSRKSMETLV FGMLKDRQKI SAVPPKEYAA RFVSFVSSII V
NT Sequence
atgccgttca atagagaaat acttgccgcc atctcctttg aatctggacc cctttcctca gcggagatag cggcacgtcg cactcctacg gaggaaccgc caaagaacct cgaagtcaag tcttgcgata aaagagatga gccgaggcaa aatgaggcga cgattaactt gatacgctgt atgaaatggg cgttggggca gagcatcacg agcatctccc atccggccaa acagcggccg ctaaacccaa agcgagattt ctccataaaa acaaagatta atctggatgt gggggtagaa aaacctaagc caggtttctg ttgcagcacg gcggcggcaa ctggtgtaag ggaacagcgt aaccaccatg caccctttta cgatgcacat ggtgacagtg aggaggcaag tcatttagat gaaagtgatg tggagtccca cgatgaggtc ccgaatgtgc cgttagcatt tgtggactac tcgcccatgt gctatcgtca cattcgggaa tttttcaata tcgaccatag ggcctaccgc gatgtcctgt tgcgaagccg ctggcacagc acaccgacac caggtaagtc cgccgcgcaa ctattcttct gtggtaggga ttgggtgatc aagaccatga cacacgaaga gagcaagttc ttacggcgca tattacatcg ctactatttt tttgtgctag accatcctta tacgctccta ccacactttg tcggtcacca ccggcttgaa atcaacggag agaagatgta cattatcatt atgcagaatg tgttcgtcac aggaaacaca atccatgaaa aatacgacct gaaggggagt acggtcggtc gttttgcgac tgaggcggaa aagaaaaagc agacatgcac acaaaaggat ttggatataa atcgtcccct tcatgtgggc gccactaggc gtaaccttct cattgaacaa atgaaaatgg actgtggatt cctgaaccag tgtaatatca tggactactc gtttcttgta ggtattcacg tgcttcccat gtacgaggca catcgcccag caccaagcac gggaagcaga gagtacttgg gtggggcagc agcgcttgca acgagtgcac tggaaccttc ctatctgtta ggcatgggtg agacgatgca acccatggct ttgaatggac atccaaccgc ggctgccgac agcgcaccaa ccgacgggcg gtgcttcaaa gcggatcagg gcggtatgat gagcgcagac caccccggtg gaaggaggga aatatattat attgggatca ttgacatatt gcaggagtac aattctcgga agagtatgga aacccttgtg ttcggaatgt tgaaagacag gcagaagata tcggcggtgc cgccgaagga atacgcagcg cgattcgtca gttttgtctc gtccattatt gtgtaa
Details for TrbrA.19310.a.B1.GE42090
HARVESTED ON: 12/5/2017
SEQUENCED ON: 12/7/2017
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMP FNREILAAIS FESGPLSSAE IAARRTPTEE PPKNLEVKSC DKRDEPRQNE ATINLIRCMK WALGQSITSI SHPAKQRPLN PKRDFSIKTK INLDVGVEKP KPGFCCSTAA ATGVREQRNH HAPFYDAHGD SEEASHLDES DVESHDEVPN VPLAFVDYSP MCYRHIREFF NIDHRAYRDV LLRSRWHSTP TPGKSAAQLF FCGRDWVIKT MTHEESKFLR RILHRYYFFV LDHPYTLLPH FVGHHRLEIN GEKMYIIIMQ NVFVTGNTIH EKYDLKGSTV GRFATEAEKK KQTCTQKDLD INRPLHVGAT RRNLLIEQMK MDCGFLNQCN IMDYSFLVGI HVLPMYEAHR PAPSTGSREY LGGAAALATS ALEPSYLLGM GETMQPMALN GHPTAAADSA PTDGRCFKAD QGGMMSADHP GGRREIYYIG IIDILQEYNS RKSMETLVFG MLKDRQKISA VPPKEYAARF VS
Validated NT Sequence
aaactgacga atcgcgctgc gtattccttc ggcggcaccg ccgatatctt ctgcctgtct ttcaacattc cgaacacaag ggtttccata ctcttccgag aattgtactc ctgcaatatg tcaatgatcc caatataata tatttccctc cttccaccgg ggtggtctgc gctcatcata ccgccctgat ccgctttgaa gcaccgcccg tcggttggtg cgctgtcggc agccgcggtt ggatgtccat tcaaagccat gggttgcatc gtctcaccca tgcctaacag ataggaaggt tccagtgcac tcgttgcaag cgctgctgcc ccacccaagt actctctgct tcccgtgctt ggtgctgggc gatgtgcctc gtacatggga agcacgtgaa tacctacaag aaacgagtag tccatgatat tacactggtt caggaatcca cagtccattt tcatttgttc aatgagaagg ttacgcctag tggcgcccac atgaagggga cgatttatat ccaaatcctt ttgtgtgcat gtctgctttt tcttttccgc ctcagtcgca aaacgaccga ccgtactccc cttcaggtcg tatttttcat ggattgtgtt tcctgtgacg aacacattct gcataatgat aatgtacatc ttctctccgt tgatttcaag ccggtggtga ccgacaaagt gtggtaggag cgtataagga tggtctagca caaaaaaata gtagcgatgc aatatgcgcc gtaagaactt gctctcttcg tgtgtcatgg tcttgatcac ccaatcccta ccacagaaga atagttgcgc ggcggactta cctggtgtcg gtgtgctgtg ccagcggctt cgcaacagga catcgcggta ggccctatgg tcgatattga aaaattcccg aatgtgacga tagcacatgg gtgagtagtc cacaaatgct aacggcacat tcgggacctc atcgtgggac tccacgtcac tttcatctaa atgacttgcc tcctcactgt caccatgtgc atcgtaaaag ggtgcatggt ggttacgctg ttcccttaca ccagttgccg ccgccgtgct gcaacagaaa cccggcttag gtttttctac ccccacatcc agattaatct ttgtttttat ggagaaatct cgctttgggt ttagcggccg ctgtttggcc ggatgggaga tgctcgtgat gctctgcccc aacgcccatt tcatacagcg tatcaagtta atcgtcgcct cattttgcct cggctcatct cttttatcgc aagacttgac ttcgaggttc tttggcggtt cctccgtagg agtgcgacgt gccgctatct ccgctgagga aaggggtcca gattcaaagg agatggcggc aagtatttct ctattgaacg gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMP FNREILAAIS FESGPLSSAE IAARRTPTEE PPKNLEVKSC DKRDEPRQNE ATINLIRCMK WALGQSITSI SHPAKQRPLN PKRDFSIKTK INLDVGVEKP KPGFCCSTAA ATGVREQRNH HAPFYDAHGD SEEASHLDES DVESHDEVPN VPLAFVDYSP MCYRHIREFF NIDHRAYRDV LLRSRWHSTP TPGKSAAQLF FCGRDWVIKT MTHEESKFLR RILHRYYFFV LDHPYTLLPH FVGHHRLEIN GEKMYIIIMQ NVFVTGNTIH EKYDLKGSTV GRFATEAEKK KQTCTQKDLD INRPLHVGAT RRNLLIEQMK MDCGFLNQCN IMDYSFLVGI HVLPMYEAHR PAPSTGSREY LGGAAALATS ALEPSYLLGM GETMQPMALN GHPTAAADSA PTDGRCFKAD QGGMMSADHP GGRREIYYIG IIDILQEYNS RKSMETLVFG MLKDRQKISA VPPKEYAARF VSFVSSIIV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgt tcaatagaga aatacttgcc gccatctcct ttgaatctgg acccctttcc tcagcggaga tagcggcacg tcgcactcct acggaggaac cgccaaagaa cctcgaagtc aagtcttgcg ataaaagaga tgagccgagg caaaatgagg cgacgattaa cttgatacgc tgtatgaaat gggcgttggg gcagagcatc acgagcatct cccatccggc caaacagcgg ccgctaaacc caaagcgaga tttctccata aaaacaaaga ttaatctgga tgtgggggta gaaaaaccta agccaggttt ctgttgcagc acggcggcgg caactggtgt aagggaacag cgtaaccacc atgcaccctt ttacgatgca catggtgaca gtgaggaggc aagtcattta gatgaaagtg atgtggagtc ccacgatgag gtcccgaatg tgccgttagc atttgtggac tactcgccca tgtgctatcg tcacattcgg gaatttttca atatcgacca tagggcctac cgcgatgtcc tgttgcgaag ccgctggcac agcacaccga caccaggtaa gtccgccgcg caactattct tctgtggtag ggattgggtg atcaagacca tgacacacga agagagcaag ttcttacggc gcatattaca tcgctactat ttttttgtgc tagaccatcc ttatacgctc ctaccacact ttgtcggtca ccaccggctt gaaatcaacg gagagaagat gtacattatc attatgcaga atgtgttcgt cacaggaaac acaatccatg aaaaatacga cctgaagggg agtacggtcg gtcgttttgc gactgaggcg gaaaagaaaa agcagacatg cacacaaaag gatttggata taaatcgtcc ccttcatgtg ggcgccacta ggcgtaacct tctcattgaa caaatgaaaa tggactgtgg attcctgaac cagtgtaata tcatggacta ctcgtttctt gtaggtattc acgtgcttcc catgtacgag gcacatcgcc cagcaccaag cacgggaagc agagagtact tgggtggggc agcagcgctt gcaacgagtg cactggaacc ttcctatctg ttaggcatgg gtgagacgat gcaacccatg gctttgaatg gacatccaac cgcggctgcc gacagcgcac caaccgacgg gcggtgcttc aaagcggatc agggcggtat gatgagcgca gaccaccccg gtggaaggag ggaaatatat tatattggga tcattgacat attgcaggag tacaattctc ggaagagtat ggaaaccctt gtgttcggaa tgttgaaaga caggcagaag atatcggcgg tgccgccgaa ggaatacgca gcgcgattcg tcagttttgt ctcgtccatt attgtgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t