SSGCID: Target Page

TrbrA.18896.a

threonyl-tRNA synthetase, putative

CENTER ID:	TrbrA.18896.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.18896.a.B1.GE42777	full length		1	799

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.5.1090
UniProt:	Q57W54

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATAVVGKKK GGATVDLKQL QEPDFWKTRN AIFDELYAAQ QEKYKSMQSP ISITLNDGKQ LPATSWLTTP IDIAKKLSNS LAERVVAARV NEEIWDLTRP FEGDATLELL DWDDADARHV FWHSSSHVLG YALERVFQTR LSVGPALEEG GFFYEGETNR PVTEADYTTI ETAMQELVKM KVPFQRLTVK KEDALRLFQY TEFKSKILAS KVPENGTCTV YRCGNLIDPC RGPHLPDTGR VKSFSLTKNS SSYFEGKAEN AVLQRVYGIS FPKQTMLTEW KKLQEEAARR DHRTIGRHQQ LFHFHEASPG NAFWLPHGAR IYNTLIEFQR KQYRRRGFEE VVSPNMFSSR LWMVSGHWDK YADNMFLINV EKEDHGMKPM NCPGHCLMFA MQPHSYRELP IRYADFGVLH RNELSGALTG LTRVRRFQQD DAHIFCRMDQ VKDEIHSALI FLKDVYDVLG FKFFLKHSTR PENKLGSDEM WDEAESYLQA ALNSFCGIPD ELPDPFNKGS TFVYDGRKES VKKLRAMLKK RGDKKNDENS PADDVWPGPT HEDAWEENTG DGAFYGPKID IVVEDALRRR HQCATIQLDF NLPKRFGLKY TLPSNAAGDD GPAPKATECH KDPNAELKAD KPEGKVEAAA GAPKSSYEKA AHELNIDQRL DANQARPVMI HRAIFGSLER CIAILCEHYG GDWPFWLSPR QVIVVPVSLE NAAYAQTVRD TFFAAGFFAD VDNGTATLEK KIRNAELARY NFILVVGQAE QEGQSVNVRT RDNRRHGTKT LDEALQWLRE LADKYDPSF

NT Sequence

atggccacgg ctgttgttgg gaagaaaaag ggaggtgcaa ctgtcgatct caaacagcta caggaacctg acttttggaa gacgcgtaat gccatatttg acgaattgta tgccgcccag caggagaaat ataaatccat gcagagcccc atttccatca ccctcaatga tggaaagcag ctccctgcca cgagctggct cacaacgcct attgacattg cgaagaagtt gtccaattcg ctcgcggaaa gggttgttgc tgcgcgtgta aatgaagaaa tttgggacct tactcgacct tttgagggcg atgccacact tgagctgctt gattgggacg atgctgatgc tcgccacgtt ttttggcaca gttcctccca cgtgcttggt tatgcgctgg agcgagtgtt ccaaacccgc ctgtccgttg gccctgcact tgaggagggt ggtttcttct atgagggtga gacgaaccgc ccggtgacgg aagcggatta caccacgata gagacagcga tgcaggagtt ggtcaagatg aaggttccat ttcagcgctt aaccgtaaag aaggaggacg cccttcgact cttccagtac acagagttta agagcaagat actcgcaagt aaggttcctg agaatggcac ttgcacggtg tatcgctgcg ggaaccttat tgacccctgc cgcggtcctc acctccccga caccgggcgc gtgaagtcat tctccttaac aaagaactcc tcttcgtact ttgaggggaa ggcggagaat gctgtgttac agcgtgtcta tggaatttca ttcccaaagc agacgatgtt aacggagtgg aaaaaacttc aagaagaggc cgcccgtcgc gaccaccgca ctattggccg gcatcaacag ctcttccatt tccatgaggc ctctcccggt aacgctttct ggctcccgca tggtgcacgc atctacaaca cactaattga gttccagcgc aagcaatatc gccgacgggg cttcgaggag gtagtgtctc ctaacatgtt ctcatccagg ttgtggatgg tctccggtca ctgggataag tatgctgaca atatgttcct catcaatgtt gaaaaggagg accacggaat gaagccaatg aactgtcctg gacactgcct catgtttgcc atgcagcccc actcgtacag ggagcttcct attcgttatg ctgattttgg ggttctgcat cgaaatgagc tcagcggtgc tcttacaggg ctcacccgag ttcgtcgttt tcagcaggat gatgcccaca ttttctgtcg gatggatcag gtgaaggatg agatccacag cgctctcatt tttctcaagg atgtttatga cgtcctcggc tttaagttct ttttgaagca ctccacgcgg ccagaaaaca agctcggttc agatgagatg tgggatgagg cggagtccta cttgcaggca gccctcaaca gcttctgcgg gatccccgac gagcttcctg acccatttaa caagggttcc acctttgtat acgatggccg caaggagagc gttaaaaaac ttcgggcaat gctgaagaag cgtggagaca agaaaaacga cgagaactca cccgcagatg atgtgtggcc gggcccaaca catgaggatg catgggagga aaacaccggt gacggtgctt tctatgggcc aaagattgac atcgttgttg aagatgcttt gcgccgacgg catcaatgtg cgaccatcca gcttgacttc aacttaccga agcggtttgg tctcaagtac actctgccga gcaatgcagc gggcgatgat ggcccagcgc caaaggccac cgagtgccac aaagatccga acgcggaatt aaaagctgac aaaccggagg ggaaggtgga ggctgctgcg ggagcaccga agtcgtccta cgagaaggct gcacacgaac tcaacattga ccagcgtttg gatgccaatc aggctcgacc tgtgatgatt caccgtgcca ttttcggttc tttggagcgc tgcattgcga tcctctgcga gcactacggc ggtgactggc ccttctggct gagcccgcgg caagttattg tcgtgcctgt ttcgctcgag aacgctgcgt acgcacaaac ggtgcgcgac acgttttttg ctgcaggctt ctttgctgat gtggacaacg gcactgcgac gttggagaag aagattcgga atgcggaact ggcacggtac aacttcatac ttgttgtggg acaagctgag caagaggggc agagtgtcaa tgtgaggact cgtgacaatc gccggcacgg cacgaaaacg ttggatgagg cattacaatg gttgcgagaa ttggccgata aatatgatcc gtccttctaa

Details for TrbrA.18896.a.B1.GE42777

HARVESTED ON:	11/5/2018
SEQUENCED ON:	11/7/2018
EXPECTED MW:	92kDa
OBSERVED MW:	92kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA TAVVGKKKGG ATVDLKQLQE PDFWKTRNAI FDELYAAQQE KYKSMQSPIS ITLNDGKQLP ATSWLTTPID IAKKLSNSLA ERVVAARVNE EIWDLTRPFE GDATLELLDW DDADARHVFW HSSSHVLGYA LERVFQTRLS VGPALEEGGF FYEGETNRPV TEADYTTIET AMQELVKMKV PFQRLTVKKE DALRLFQYTE FKSKILASKV PENGTCTVYR CGNLIDPCRG PHLPDTGRVK SFSLTKNSSS YFEGKAENAV LQRVYGISFP KQTMLTEWKK LQEEAARRDH RTIGRHQQLF HFHEASPGNA FWLPHGARIY NTLIEFQRKQ YRRRGFEEVV SPNMFSSRLW MVSGHWDKYA DNMFLINVEK EDHGMKPMNC PGHCLMFAMQ PHSYRELPIR YADFGVLHRN ELSGALTGLT RVRRFQQDDA HIFCRMDQVK DEIHSALIFL KDVYDVLGFK FFLKHSTRPE NKLGSDEMWD EAESYLQAAL NSFCGIPDEL PDPFNKGSTF VYDGRKESVK KLRAMLKKRG DKKNDENSPA DDVWPGPTHE DAWEENTGDG AFYGPKIDIV VEDALRRRHQ CATIQLDFNL PKRFGLKYTL PSNAAGDDGP APKATECHKD PNAELKADKP EGKVEAAAGA PKSSYEKAAH ELNIDQRLDA NQARPVMIHR AIFGSLERCI AILCEHYGGD WPFWLSPRQV IVVPVSLENA AYAQTVRDTF FAAGFFADVD NGTATLEKKI RNAELARYNF ILVVGQAEQE GQSVNVRTRD NRRHGTKTLD EALQWLRELA DKYDPSF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca cggctgttgt tgggaagaaa aagggaggtg caactgtcga tctcaaacag ctacaggaac ctgacttttg gaagacgcgt aatgccatat ttgacgaatt gtatgccgcc cagcaggaga aatataaatc catgcagagc cccatttcca tcaccctcaa tgatggaaag cagctccctg ccacgagctg gctcacaacg cctattgaca ttgcgaagaa gttgtccaat tcgctcgcgg aaagggttgt tgctgcgcgt gtaaatgaag aaatttggga ccttactcga ccttttgagg gcgatgccac acttgagctg cttgattggg acgatgctga tgctcgccac gttttttggc acagttcctc ccacgtgctt ggttatgcgc tggagcgagt gttccaaacc cgcctgtccg ttggccctgc acttgaggag ggtggtttct tctatgaggg tgagacgaac cgcccggtga cggaagcgga ttacaccacg atagagacag cgatgcagga gttggtcaag atgaaggttc catttcagcg cttaaccgta aagaaggagg acgcccttcg actcttccag tacacagagt ttaagagcaa gatactcgca agtaaggttc ctgagaatgg cacttgcacg gtgtatcgct gcgggaacct tattgacccc tgccgcggtc ctcacctccc cgacaccggg cgcgtgaagt cattctcctt aacaaagaac tcctcttcgt actttgaggg gaaggcggag aatgctgtgt tacagcgtgt ctatggaatt tcattcccaa agcagacgat gttaacggag tggaaaaaac ttcaagaaga ggccgcccgt cgcgaccacc gcactattgg ccggcatcaa cagctcttcc atttccatga ggcctctccc ggtaacgctt tctggctccc gcatggtgca cgcatctaca acacactaat tgagttccag cgcaagcaat atcgccgacg gggcttcgag gaggtagtgt ctcctaacat gttctcatcc aggttgtgga tggtctccgg tcactgggat aagtatgctg acaatatgtt cctcatcaat gttgaaaagg aggaccacgg aatgaagcca atgaactgtc ctggacactg cctcatgttt gccatgcagc cccactcgta cagggagctt cctattcgtt atgctgattt tggggttctg catcgaaatg agctcagcgg tgctcttaca gggctcaccc gagttcgtcg ttttcagcag gatgatgccc acattttctg tcggatggat caggtgaagg atgagatcca cagcgctctc atttttctca aggatgttta tgacgtcctc ggctttaagt tctttttgaa gcactccacg cggccagaaa acaagctcgg ttcagatgag atgtgggatg aggcggagtc ctacttgcag gcagccctca acagcttctg cgggatcccc gacgagcttc ctgacccatt taacaagggt tccacctttg tatacgatgg ccgcaaggag agcgttaaaa aacttcgggc aatgctgaag aagcgtggag acaagaaaaa cgacgagaac tcacccgcag atgatgtgtg gccgggccca acacatgagg atgcatggga ggaaaacacc ggtgacggtg ctttctatgg gccaaagatt gacatcgttg ttgaagatgc tttgcgccga cggcatcaat gtgcgaccat ccagcttgac ttcaacttac cgaagcggtt tggtctcaag tacactctgc cgagcaatgc agcgggcgat gatggcccag cgccaaaggc caccgagtgc cacaaagatc cgaacgcgga attaaaagct gacaaaccgg aggggaaggt ggaggctgct gcgggagcac cgaagtcgtc ctacgagaag gctgcacacg aactcaacat tgaccagcgt ttggatgcca atcaggctcg acctgtgatg attcaccgtg ccattttcgg ttctttggag cgctgcattg cgatcctctg cgagcactac ggcggtgact ggcccttctg gctgagcccg cggcaagtta ttgtcgtgcc tgtttcgctc gagaacgctg cgtacgcaca aacggtgcgc gacacgtttt ttgctgcagg cttctttgct gatgtggaca acggcactgc gacgttggag aagaagattc ggaatgcgga actggcacgg tacaacttca tacttgttgt gggacaagct gagcaagagg ggcagagtgt caatgtgagg actcgtgaca atcgccggca cggcacgaaa acgttggatg aggcattaca atggttgcga gaattggccg ataaatatga tccgtccttc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat