SSGCID: Target Page

TrbrA.18809.a

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34)

CENTER ID:	TrbrA.18809.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.18809.a.B2.GE41047	soluble domain		1	401

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.6.4540
UniProt:	Q587C0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRRTLSCAC GTGKTGWGAM SNAELVKAVS SRQLTFYGLE QALEPDYQRA IAVRREVVSD YVASSQSAEV KRKSLERIPF ENYEWDRVVG QNCENIVGYV PIPLGMAGPI IMDGCEYPIP MATTEGALVA STHRGARAIS QSGGCKTLIL GEGMSRAPVV EVESLEEAGR LHNFCVENFT EIKAAFESTT RFGKLQSLKC VIIGRKAYIR FRATTGDAMG MNMITKGVDK ALELIKQNFP SMKVIALSGN YCTDKKPSAV NWIEGRGKTV VAEALVRGEL VERTLKCTVE DLVSLNIDKN LVGSAVAGSV GGFNAQAANV VAAIFIATGQ DPAQVVESST CITTMSKVGN DLLISVAMPS IEVGTVGGGT GLPAQSGCLD MIGCAGSNKE APGANAQLLS RLVAAGVLSA ELSLMSGLAA GHLLSAHMRL NRRK

NT Sequence

atgcttcgta ggactctctc atgtgcttgt gggacaggca aaacgggttg gggcgcaatg agcaatgccg agcttgttaa agccgtaagc agtcgtcaat taacctttta tgggttagag caggctctgg agcccgacta ccagcgcgct atcgctgtgc gtcgtgaggt ggtgtccgac tatgtggcat cctcccaaag tgcggaggtg aagaggaaaa gcttggagcg tataccattt gaaaactacg aatgggatcg agtggttggt cagaactgtg aaaatattgt gggttacgtt cctattcctc ttggtatggc tgggccaata ataatggatg gctgtgaata ccccattccc atggccacga cagaaggtgc cctggttgcc agcacacatc gaggcgcacg agctatatcc caaagtgggg gctgtaagac actgatcttg ggcgagggca tgtcacgtgc tccagtagtg gaggttgagt ctttggaaga ggcgggaagg cttcataatt tttgtgtaga aaatttcact gaaatcaaag cagctttcga gagcacgacg cgatttggga agttgcagtc gctcaagtgc gtcatcatcg gtcgcaaagc ctatattcgg ttccgcgcga ccaccggaga tgcgatgggg atgaacatga taaccaaagg tgtcgacaag gcattggagt taataaagca aaactttccg tcgatgaagg ttattgcgtt gtcggggaac tactgcactg acaagaaacc ctccgcagtg aactggattg aggggagagg aaaaactgtc gttgccgagg cgctcgtaag gggcgagttg gttgagcgca cattaaagtg caccgttgag gatcttgtgt cactaaatat cgacaaaaac ctcgttggct cagctgttgc cggttctgtt ggtggcttta atgcacaagc tgccaatgtt gttgcagcaa ttttcattgc gacggggcag gacccagcgc aggtggtgga atcctccacc tgcatcacca caatgtctaa agttgggaac gatctgctca tttcggtcgc aatgcccagc attgaggtgg gaacagttgg tggtggcacg ggtctaccag cccagagtgg gtgcttagat atgatcgggt gtgctgggtc gaacaaggaa gcaccagggg ccaacgcgca gcttctctca cgtcttgttg cggctggtgt tctttctgcg gaactgtcgc tcatgtcagg gttggctgca gggcacctcc taagtgccca catgcggttg aatcgtagga agtaa

Details for TrbrA.18809.a.B2.GE41047

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML RRTLSCACGT GKTGWGAMSN AELVKAVSSR QLTFYGLEQA LEPDYQRAIA VRREVVSDYV ASSQSAEVKR KSLERIPFEN YEWDRVVGQN CENIVGYVPI PLGMAGPIIM DGCEYPIPMA TTEGALVAST HRGARAISQS GGCKTLILGE GMSRAPVVEV ESLEEAGRLH NFCVENFTEI KAAFESTTRF GKLQSLKCVI IGRKAYIRFR ATTGDAMGMN MITKGVDKAL ELIKQNFPSM KVIALSGNYC TDKKPSAVNW IEGRGKTVVA EALVRGELVE RTLKCTVEDL VSLNIDKNLV GSAVAGSVGG FNAQAANVVA AIFIATGQDP AQVVESSTCI TTMSKVGNDL LISVAMPSIE VGTVGGGTGL PAQSGCLDMI GCAGSNKEAP GA

Validated NT Sequence

tggcccctgg tgcttccttg ttcgacccag cacacccgat catatctaag cacccactct gggctggtag acccgtgcca ccaccaactg ttcccacctc aatgctgggc attgcgaccg aaatgagcag atcgttccca actttagaca ttgtggtgat gcaggtggag gattccacca cctgcgctgg gtcctgcccc gtcgcaatga aaattgctgc aacaacattg gcagcttgtg cattaaagcc accaacagaa ccggcaacag ctgagccaac gaggtttttg tcgatattta gtgacacaag atcctcaacg gtgcacttta atgtgcgctc aaccaactcg ccccttacga gcgcctcggc aacgacagtt tttcctctcc cctcaatcca gttcactgcg gagggtttct tgtcagtgca gtagttcccc gacaacgcaa taaccttcat cgacggaaag ttttgcttta ttaactccaa tgccttgtcg acacctttgg ttatcatgtt catccccatc gcatctccgg tggtcgcgcg gaaccgaata taggctttgc gaccgatgat gacgcacttg agcgactgca acttcccaaa tcgcgtcgtg ctctcgaaag ctgctttgat ttcagtgaaa ttttctacac aaaaattatg aagccttccc gcctcttcca aagactcaac ctccactact ggagcacgtg acatgccctc gcccaagatc agtgtcttac agcccccact ttgggatata gctcgtgcgc ctcgatgtgt gctggcaacc agggcacctt ctgtcgtggc catgggaatg gggtattcac agccatccat tattattggc ccagccatac caagaggaat aggaacgtaa cccacaatat tttcacagtt ctgaccaacc actcgatccc attcgtagtt ttcaaatggt atacgctcca agcttttcct cttcacctcc gcactttggg aggatgccac atagtcggac accacctcac gacgcacagc gatagcgcgc tggtagtcgg gctccagagc ctgctctaac ccataaaagg ttaattgacg actgcttacg gctttaacaa gctcggcatt gctcattgcg ccccaacccg ttttgcctgt cccacaagca catgagagag tcctacgaag catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttc gtaggactct ctcatgtgct tgtgggacag gcaaaacggg ttggggcgca atgagcaatg ccgagcttgt taaagccgta agcagtcgtc aattaacctt ttatgggtta gagcaggctc tggagcccga ctaccagcgc gctatcgctg tgcgtcgtga ggtggtgtcc gactatgtgg catcctccca aagtgcggag gtgaagagga aaagcttgga gcgtatacca tttgaaaact acgaatggga tcgagtggtt ggtcagaact gtgaaaatat tgtgggttac gttcctattc ctcttggtat ggctgggcca ataataatgg atggctgtga ataccccatt cccatggcca cgacagaagg tgccctggtt gccagcacac atcgaggcgc acgagctata tcccaaagtg ggggctgtaa gacactgatc ttgggcgagg gcatgtcacg tgctccagta gtggaggttg agtctttgga agaggcggga aggcttcata atttttgtgt agaaaatttc actgaaatca aagcagcttt cgagagcacg acgcgatttg ggaagttgca gtcgctcaag tgcgtcatca tcggtcgcaa agcctatatt cggttccgcg cgaccaccgg agatgcgatg gggatgaaca tgataaccaa aggtgtcgac aaggcattgg agttaataaa gcaaaacttt ccgtcgatga aggttattgc gttgtcgggg aactactgca ctgacaagaa accctccgca gtgaactgga ttgaggggag aggaaaaact gtcgttgccg aggcgctcgt aaggggcgag ttggttgagc gcacattaaa gtgcaccgtt gaggatcttg tgtcactaaa tatcgacaaa aacctcgttg gctcagctgt tgccggttct gttggtggct ttaatgcaca agctgccaat gttgttgcag caattttcat tgcgacgggg caggacccag cgcaggtggt ggaatcctcc acctgcatca ccacaatgtc taaagttggg aacgatctgc tcatttcggt cgcaatgccc agcattgagg tgggaacagt tggtggtggc acgggtctac cagcccagag tgggtgctta gatatgatcg ggtgtgctgg gtcgaacaag gaagcaccag gggccaacgc gcagcttctc tcacgttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t