TrbrA.18754.a

phenylalanyl-tRNA synthetase (beta subunit), putative

CENTER ID: TrbrA.18754.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.18754.a.B1.GE42776 full length 1 624
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tb927.11.2360
UniProt: Q386S3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTLAVVRDY LFDFIGKSYT EDQFEDICFQ FGVELDDVTS EREMFRREQG EAVAAKGEEL SDAVIYKIDT PANRYDLLCA EGMGTAMKVF LGMTPAPRFQ LLNRANPLYK VTVEKSVRNV RDYIVCAVLR NVRLDKRSYR SFIDFQDKLH LGLARRRALA SVGTHDLDKI NQNEFLYAAR PKESIRFVPL NQQERVLDCR GNGLAEFYKD DRHISKFIPL ISEFSHYPVV LDGKGENILS LPPIINSDYS CISANTRNIF IECTAPDHHK ASVLVNQLVC AFSTYCEKPF TVEAVRVVYD EPTPDGTKEE VTPNLDPRLV SVEQSKVEKL IGIKLNSANH MGELLERMLH HVAKVEDSTV TVEVPPTRPD VLGPTDLMED VAVAYGYDNI THKECLTFGD VAQQPLSKLS HLIRIEMANA GYMELLTFSL CSREEGFTRL NRVDDDVAVH IANPKTMEFQ ICRPSLMPGI LKTLNANKSH PLPLRFFECA DIVLLDNEKN FPPVITPKLE YPNCGARNQR HLAALHCCSE SSSFEDIHGL VEFVMAKLGV VRKVGEEVVD GDSYTLERSS DGAFFPGRCM DVFLHRSGQK VCIGGFGVVH PNTLKAYDIP FPCSYMELNV QFAQ
NT Sequence
atgccaaccc tcgctgttgt gcgtgattat ctctttgatt ttatcgggaa aagctacacg gaggaccaat tcgaagacat atgctttcaa tttggtgtgg agctcgatga tgtgacaagt gagcgcgaga tgttccgccg tgagcaaggc gaagccgttg cagccaaggg agaggagctt tctgatgccg tcatctacaa gattgataca ccagccaatc gctacgacct tctttgtgct gagggaatgg gtactgcgat gaaggtattt ctcggcatga cccctgcgcc tcggttccaa ttactcaacc gtgcaaatcc actctacaaa gtaacggtgg aaaagtccgt gcgcaatgta cgggattaca ttgtttgtgc cgtgttacga aacgttcgtc ttgacaagcg cagctaccgc agttttattg attttcagga caaactccac ttgggtcttg cacgacgccg tgcgcttgct tctgttggga cgcacgactt ggataagatt aatcagaatg agttccttta cgccgcccgg ccgaaggaga gcatacggtt tgttccactc aatcaacagg aacgggtgct ggattgtcgg ggcaatggcc tcgcagagtt ttataaggac gaccgacaca tctctaagtt tattccgctt atctctgaat tctcccatta ccccgtggtg ctggacggca agggtgaaaa cattctctcc cttccaccca ttatcaactc ggactactcg tgcatcagtg caaacaccag gaacatcttt atcgagtgca ctgcaccaga tcatcacaag gcgagtgttt tagtgaacca actcgtttgc gccttctcaa cttattgcga gaagcccttt accgtggagg cggtgcgtgt tgtgtacgat gaacccactc ctgatggcac gaaggaggaa gtgactccca acttggatcc tcgactagtc tccgtagagc agtcgaaggt tgagaaactc attggcatta aacttaactc agccaatcat atgggtgaac ttctggagcg tatgctgcac catgtagcaa aggtggagga cagcaccgta acagtagaag tccctccaac gcgacctgat gtattgggac caacggacct tatggaagat gttgcggtgg cctacggtta cgataatatt acccacaaag agtgcttgac atttggtgac gtggcgcagc aaccactgag taaactctcg catcttattc gtatcgagat ggctaacgcc ggttatatgg agctcttgac cttttcgctt tgctcccgcg aggagggatt tactcgtcta aatcgcgtgg atgatgatgt ggccgtacat atcgcaaacc ccaaaactat ggagttccaa atatgccgcc cctcactcat gccgggcatc ctaaagacac ttaatgccaa taaatcgcac ccacttcccc ttcgattctt tgagtgcgct gatatagttc tcttggataa tgaaaagaac tttcctcctg tcatcacccc aaaactagag taccccaact gcggggcgcg caatcaacgg catcttgccg ccctacattg ctgtagtgag tctagcagtt ttgaagatat acacggactt gtggaatttg tcatggctaa acttggtgtt gtgcggaaag tgggggaaga ggttgtggat ggtgattcat acacgctgga gaggagcagt gatggtgcct tctttcctgg tcgttgcatg gatgtttttc tgcaccgcag tgggcagaag gtgtgtattg gaggctttgg tgttgttcac ccgaacacgc tgaaggcgta tgacatcccc tttccatgct catacatgga gctgaatgtt cagtttgccc agtag
Details for TrbrA.18754.a.B1.GE42776
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 71kDa
OBSERVED MW: 71kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMP TLAVVRDYLF DFIGKSYTED QFEDICFQFG VELDDVTSER EMFRREQGEA VAAKGEELSD AVIYKIDTPA NRYDLLCAEG MGTAMKVFLG MTPAPRFQLL NRANPLYKVT VEKSVRNVRD YIVCAVLRNV RLDKRSYRSF IDFQDKLHLG LARRRALASV GTHDLDKINQ NEFLYAARPK ESIRFVPLNQ QERVLDCRGN GLAEFYKDDR HISKFIPLIS EFSHYPVVLD GKGENILSLP PIINSDYSCI SANTRNIFIE CTAPDHHKAS VLVNQLVCAF STYCEKPFTV EAVRVVYDEP TPDGTKEEVT PNLDPRLVSV EQSKVEKLIG IKLNSANHMG ELLERMLHHV AKVEDSTVTV EVPPTRPDVL GPTDLMEDVA VAYGYDNITH KECLTFGDVA QQPLSKLSHL IRIEMANAGY MELLTFSLCS REEGFTRLNR VDDDVAVHIA NPKTMEFQIC RPSLMPGILK TLNANKSHPL PLRFFECADI VLLDNEKNFP PVITPKLEYP NCGARNQRHL AALHCCSESS SFEDIHGLVE FVMAKLGVVR KVGEEVVDGD SYTLERSSDG AFFPGRCMDV FLHRSGQKVC IGGFGVVHPN TLKAYDIPFP CSYMELNVQF AQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa ccctcgctgt tgtgcgtgat tatctctttg attttatcgg gaaaagctac acggaggacc aattcgaaga catatgcttt caatttggtg tggagctcga tgatgtgaca agtgagcgcg agatgttccg ccgtgagcaa ggcgaagccg ttgcagccaa gggagaggag ctttctgatg ccgtcatcta caagattgat acaccagcca atcgctacga ccttctttgt gctgagggaa tgggtactgc gatgaaggta tttctcggca tgacccctgc gcctcggttc caattactca accgtgcaaa tccactctac aaagtaacgg tggaaaagtc cgtgcgcaat gtacgggatt acattgtttg tgccgtgtta cgaaacgttc gtcttgacaa gcgcagctac cgcagtttta ttgattttca ggacaaactc cacttgggtc ttgcacgacg ccgtgcgctt gcttctgttg ggacgcacga cttggataag attaatcaga atgagttcct ttacgccgcc cggccgaagg agagcatacg gtttgttcca ctcaatcaac aggaacgggt gctggattgt cggggcaatg gcctcgcaga gttttataag gacgaccgac acatctctaa gtttattccg cttatctctg aattctccca ttaccccgtg gtgctggacg gcaagggtga aaacattctc tcccttccac ccattatcaa ctcggactac tcgtgcatca gtgcaaacac caggaacatc tttatcgagt gcactgcacc agatcatcac aaggcgagtg ttttagtgaa ccaactcgtt tgcgccttct caacttattg cgagaagccc tttaccgtgg aggcggtgcg tgttgtgtac gatgaaccca ctcctgatgg cacgaaggag gaagtgactc ccaacttgga tcctcgacta gtctccgtag agcagtcgaa ggttgagaaa ctcattggca ttaaacttaa ctcagccaat catatgggtg aacttctgga gcgtatgctg caccatgtag caaaggtgga ggacagcacc gtaacagtag aagtccctcc aacgcgacct gatgtattgg gaccaacgga ccttatggaa gatgttgcgg tggcctacgg ttacgataat attacccaca aagagtgctt gacatttggt gacgtggcgc agcaaccact gagtaaactc tcgcatctta ttcgtatcga gatggctaac gccggttata tggagctctt gaccttttcg ctttgctccc gcgaggaggg atttactcgt ctaaatcgcg tggatgatga tgtggccgta catatcgcaa accccaaaac tatggagttc caaatatgcc gcccctcact catgccgggc atcctaaaga cacttaatgc caataaatcg cacccacttc cccttcgatt ctttgagtgc gctgatatag ttctcttgga taatgaaaag aactttcctc ctgtcatcac cccaaaacta gagtacccca actgcggggc gcgcaatcaa cggcatcttg ccgccctaca ttgctgtagt gagtctagca gttttgaaga tatacacgga cttgtggaat ttgtcatggc taaacttggt gttgtgcgga aagtggggga agaggttgtg gatggtgatt catacacgct ggagaggagc agtgatggtg ccttctttcc tggtcgttgc atggatgttt ttctgcaccg cagtgggcag aaggtgtgta ttggaggctt tggtgttgtt cacccgaaca cgctgaaggc gtatgacatc ccctttccat gctcatacat ggagctgaat gttcagtttg cccagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat