TrbrA.18376.a

trypanothione synthetase

CENTER ID: TrbrA.18376.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.18376.a.A1.GE34299 full length 1 627
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.18376.a.A1.PW34907 full length 1 627

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.2.4370
RefSeq: XP_951630.1
UniProt: Q586P2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKSALADTK EEPHVPFGEI QGYTPCGVPA YSNGHDGFFS GERSIDGNLF CGFKYQCVEF ARRWLYEAKG LVLPDVNWAA HIFDLTEVHD ASTATPVPCV KVSNGTAAKP VADSLLIYAV NEDAPWGHVA VITEVGDKWV RIADQNHRFH KWKGTYSAEL LLKHEGGVWT VEDHAAEGIF VPLGWVTFPS RPNRNPKEPL VLHESLYFKQ PEKPFLRRVV FTPENRKTDW LDLTNEAEAE FYKTFGKEAT RGGVYESCYY LMNRELYLNC VRYGTQLHSF FLEATKQVLE SDDKLRRFRI PEEYWPRIRH SWKTQPHAIT GRFDFVFDEN TQEFKCFEYN ADSASTLLEC AVIQEKWANS VGLDDNATRS SGKFMPQTLV RAWEMTGLKG RVHFLVDDDG EERYTALYVM EKAREAGIDA KLCVMFDEFH FDEKGAVVDS DGIPVTAVWK TWMWETAISD HQAAREQRGA EWKPTPKDKV RLCDILLGNN WDIRVFEPMW KLIPSNKAIL PIIYNNHPDH PAILPASYEL TDELRRTGYA KKPIVGRVGR NVTVTEPDGK VLAESDGNFS NRDMVYQQLF RIPKRGDYYA ILGGWMLGDT YSGTGVREDK KLITGLESPF GPVRIQM
NT Sequence
atgacgaagt cggcacttgc agacactaaa gaagaacctc acgttccctt tggtgagatt cagggctaca ccccttgtgg cgtacctgct tacagcaacg gacatgatgg ctttttttcg ggagagcgca gtattgacgg gaatcttttc tgcggtttca aataccaatg tgtagaattt gctcgtcgtt ggttatatga ggccaaaggg cttgttcttc cagacgttaa ttgggctgct cacatatttg atctaacaga ggttcatgac gcaagcactg caacccccgt cccgtgcgtt aaagtttcca acggaacagc agcgaagccc gtggcagatt cgttgctgat ttacgccgtt aatgaggatg cgccatgggg acatgtcgcg gttatcacag aggtgggtga caaatgggtg cgcattgcgg accaaaacca tcggtttcat aagtggaagg ggacctactc ggcagagttg ttgctgaagc acgaaggagg tgtgtggact gttgaggatc atgcagcaga gggaatcttt gttcctctgg ggtgggttac attccctagt aggccaaacc gtaatcctaa ggaaccattg gtgttgcatg agtcacttta ctttaaacaa ccagagaagc ctttccttcg tcgcgttgtt ttcactccag agaatcgaaa gactgactgg ctggacttga caaacgaagc cgaagcggaa ttctacaaaa catttggtaa ggaggcgact cgaggagggg tgtacgaatc atgttactat ttgatgaaca gggagttgta ccttaactgc gtccgttacg gtacgcagct gcattcattc tttctcgagg caacaaagca ggtgcttgag agcgacgata agttacgtcg atttcgcatc ccagaagagt attggcctcg catccggcac tcatggaaaa ctcagcccca tgccatcacg gggcggtttg actttgtttt tgatgaaaac actcaagaat tcaagtgctt tgaatacaat gcggacagcg cgtcgacact tctcgaatgt gctgtaatcc aagagaagtg ggcaaactcc gttgggttag acgacaatgc tacaagaagc agcgggaaat tcatgcccca aacactcgtc cgggcgtggg aaatgacggg gttgaagggg cgtgtgcatt ttcttgtgga cgatgacggg gaggaacgtt atactgcttt gtatgtgatg gagaaggctc gagaggctgg tattgacgcg aaattgtgcg tcatgtttga cgagttccat ttcgatgaaa agggagcagt tgtggattcg gacggcattc cggtgacggc tgtgtggaag acgtggatgt gggagacggc gatttctgat catcaagctg cacgagagca gcgtggcgcg gagtggaagc ccacgccaaa ggataaggtt cgcctgtgtg atattcttct cggaaataac tgggacatac gtgtctttga gcctatgtgg aaactcattc ccagcaacaa agcaatattg cccattattt ataataatca ccccgaccac cccgccatac ttcccgcaag ttatgaactc actgatgagc ttcgccgcac cggctatgca aagaagccca tcgttggccg tgtaggaagg aatgtgaccg tcactgaacc tgacggcaaa gttctagctg agtctgatgg caacttcagt aaccgtgaca tggtgtatca acaacttttc cgcataccaa agcgaggcga ttactacgca attctcgggg gctggatgct cggtgacacc tacagtggta caggtgtacg tgaggacaaa aagcttatca ctgggctcga atctccgttt ggtcccgtac gtattcaaat gtag
Details for TrbrA.18376.a.A1.GE34299
HARVESTED ON: 12/19/2011
SEQUENCED ON: 1/3/2012
EXPECTED MW: 73kDa
OBSERVED MW: 73kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 39
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKSALADT KEEPHVPFGE IQGYTPCGVP AYSNGHDGFF SGERSIDGNL FCGFKYQCVE FARRWLYEAK GLVLPDVNWA AHIFDLTEVH DASTATPVPC VKVSNGTAAK PVAXSLLIYA VXEDAPWGHV AVITEVGXKW VRIADQNHRF HKWKGXYSAE LXLKHEGSVW TVEDHAAXGI FVPLGWVTFP SRPNRNPKEP LVLHESLXFK XQRSXXXXAL FSLXXIXXXX GWX
Validated NT Sequence
attttnnttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgacg aagtcggcac ttgcagacac taaagaagaa cctcacgttc cctttggtga gattcagggc tacacccctt gtggcgtacc tgcttacagc aacggacatg atggcttttt ttcgggagag cgcagtattg acgggaatct tttctgcggt ttcaaatacc aatgtgtaga atttgctcgt cgttggttat atgaggccaa agggcttgtt cttccagacg ttaattgggc tgctcacata tttgatctaa cagaggttca tgacgcaagc actgcaaccc ccgtcccgtg cgttaaagtt tccaacggaa cagcagcgaa gcccgtggca gantcgttgc tgatttacgc cgttnatgag gatgcgccat ggggacatgt tgcggttatt acagaggtgg gtganaaatg ggtgcgcatt gcggaccaaa accatcggtt tcataagtgg aagggganct actcggcaga gttgttnctg aagcacgaag gaagtgtgtg gactgttgag gatcatgcag canagggnat ctttgttcct ctggggtggg ttacattccc tagtaggcca aaccgtaatc ctaaggagcc attggtgttg catgagtcac tttnctttaa annncagaga agccnttnnn tcgncgcgtt gttttcnctn cannnnatcn nannantgnn tggctggant tgannaacga annnaannng nnttntannn nanattggag gcnnntcgng nnggnnnncn annnnnntan nntttgnngn nnnngnntgt annnnnnnnn ncnnnngnnn gnngctnnnt tcnttntttn nnnannannn annnnnnntn anannnanna nnnnnacntc nantnnnnnn ncnnnnnnnn nnnnnnnncn nnnnnnnnnn nnnnnnnnnn nnncnngnnn nnnnngnnnn nnnntntttt tt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKSALADT KEEPHVPFGE IQGYTPCGVP AYSNGHDGFF SGERSIDGNL FCGFKYQCVE FARRWLYEAK GLVLPDVNWA AHIFDLTEVH DASTATPVPC VKVSNGTAAK PVADSLLIYA VNEDAPWGHV AVITEVGDKW VRIADQNHRF HKWKGTYSAE LLLKHEGGVW TVEDHAAEGI FVPLGWVTFP SRPNRNPKEP LVLHESLYFK QPEKPFLRRV VFTPENRKTD WLDLTNEAEA EFYKTFGKEA TRGGVYESCY YLMNRELYLN CVRYGTQLHS FFLEATKQVL ESDDKLRRFR IPEEYWPRIR HSWKTQPHAI TGRFDFVFDE NTQEFKCFEY NADSASTLLE CAVIQEKWAN SVGLDDNATR SSGKFMPQTL VRAWEMTGLK GRVHFLVDDD GEERYTALYV MEKAREAGID AKLCVMFDEF HFDEKGAVVD SDGIPVTAVW KTWMWETAIS DHQAAREQRG AEWKPTPKDK VRLCDILLGN NWDIRVFEPM WKLIPSNKAI LPIIYNNHPD HPAILPASYE LTDELRRTGY AKKPIVGRVG RNVTVTEPDG KVLAESDGNF SNRDMVYQQL FRIPKRGDYY AILGGWMLGD TYSGTGVRED KKLITGLESP FGPVRIQM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgaagtcg gcacttgcag acactaaaga agaacctcac gttccctttg gtgagattca gggctacacc ccttgtggcg tacctgctta cagcaacgga catgatggct ttttttcggg agagcgcagt attgacggga atcttttctg cggtttcaaa taccaatgtg tagaatttgc tcgtcgttgg ttatatgagg ccaaagggct tgttcttcca gacgttaatt gggctgctca catatttgat ctaacagagg ttcatgacgc aagcactgca acccccgtcc cgtgcgttaa agtttccaac ggaacagcag cgaagcccgt ggcagattcg ttgctgattt acgccgttaa tgaggatgcg ccatggggac atgtcgcggt tatcacagag gtgggtgaca aatgggtgcg cattgcggac caaaaccatc ggtttcataa gtggaagggg acctactcgg cagagttgtt gctgaagcac gaaggaggtg tgtggactgt tgaggatcat gcagcagagg gaatctttgt tcctctgggg tgggttacat tccctagtag gccaaaccgt aatcctaagg aaccattggt gttgcatgag tcactttact ttaaacaacc agagaagcct ttccttcgtc gcgttgtttt cactccagag aatcgaaaga ctgactggct ggacttgaca aacgaagccg aagcggaatt ctacaaaaca tttggtaagg aggcgactcg aggaggggtg tacgaatcat gttactattt gatgaacagg gagttgtacc ttaactgcgt ccgttacggt acgcagctgc attcattctt tctcgaggca acaaagcagg tgcttgagag cgacgataag ttacgtcgat ttcgcatccc agaagagtat tggcctcgca tccggcactc atggaaaact cagccccatg ccatcacggg gcggtttgac tttgtttttg atgaaaacac tcaagaattc aagtgctttg aatacaatgc ggacagcgcg tcgacacttc tcgaatgtgc tgtaatccaa gagaagtggg caaactccgt tgggttagac gacaatgcta caagaagcag cgggaaattc atgccccaaa cactcgtccg ggcgtgggaa atgacggggt tgaaggggcg tgtgcatttt cttgtggacg atgacgggga ggaacgttat actgctttgt atgtgatgga gaaggctcga gaggctggta ttgacgcgaa attgtgcgtc atgtttgacg agttccattt cgatgaaaag ggagcagttg tggattcgga cggcattccg gtgacggctg tgtggaagac gtggatgtgg gagacggcga tttctgatca tcaagctgca cgagagcagc gtggcgcgga gtggaagccc acgccaaagg ataaggttcg cctgtgtgat attcttctcg gaaataactg ggacatacgt gtctttgagc ctatgtggaa actcattccc agcaacaaag caatattgcc cattatttat aataatcacc ccgaccaccc cgccatactt cccgcaagtt atgaactcac tgatgagctt cgccgcaccg gctatgcaaa gaagcccatc gttggccgtg taggaaggaa tgtgaccgtc actgaacctg acggcaaagt tctagctgag tctgatggca acttcagtaa ccgtgacatg gtgtatcaac aacttttccg cataccaaag cgaggcgatt actacgcaat tctcgggggc tggatgctcg gtgacaccta cagtggtaca ggtgtacgtg aggacaaaaa gcttatcact gggctcgaat ctccgtttgg tcccgtacgt attcaaatgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for TrbrA.18376.a.A1.PW34907
PURIFICATION DATe: 2/3/2012
CONCENTRATION: 13.99mg/ml
OBSERVED MW: 73kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 89
PERCENT COVERAGE: 39
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKSALADT KEEPHVPFGE IQGYTPCGVP AYSNGHDGFF SGERSIDGNL FCGFKYQCVE FARRWLYEAK GLVLPDVNWA AHIFDLTEVH DASTATPVPC VKVSNGTAAK PVAXSLLIYA VXEDAPWGHV AVITEVGXKW VRIADQNHRF HKWKGXYSAE LXLKHEGSVW TVEDHAAXGI FVPLGWVTFP SRPNRNPKEP LVLHESLXFK XQRSXXXXAL FSLXXIXXXX GWX
Validated NT Sequence
attttnnttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgacg aagtcggcac ttgcagacac taaagaagaa cctcacgttc cctttggtga gattcagggc tacacccctt gtggcgtacc tgcttacagc aacggacatg atggcttttt ttcgggagag cgcagtattg acgggaatct tttctgcggt ttcaaatacc aatgtgtaga atttgctcgt cgttggttat atgaggccaa agggcttgtt cttccagacg ttaattgggc tgctcacata tttgatctaa cagaggttca tgacgcaagc actgcaaccc ccgtcccgtg cgttaaagtt tccaacggaa cagcagcgaa gcccgtggca gantcgttgc tgatttacgc cgttnatgag gatgcgccat ggggacatgt tgcggttatt acagaggtgg gtganaaatg ggtgcgcatt gcggaccaaa accatcggtt tcataagtgg aagggganct actcggcaga gttgttnctg aagcacgaag gaagtgtgtg gactgttgag gatcatgcag canagggnat ctttgttcct ctggggtggg ttacattccc tagtaggcca aaccgtaatc ctaaggagcc attggtgttg catgagtcac tttnctttaa annncagaga agccnttnnn tcgncgcgtt gttttcnctn cannnnatcn nannantgnn tggctggant tgannaacga annnaannng nnttntannn nanattggag gcnnntcgng nnggnnnncn annnnnntan nntttgnngn nnnngnntgt annnnnnnnn ncnnnngnnn gnngctnnnt tcnttntttn nnnannannn annnnnnntn anannnanna nnnnnacntc nantnnnnnn ncnnnnnnnn nnnnnnnncn nnnnnnnnnn nnnnnnnnnn nnncnngnnn nnnnngnnnn nnnntntttt tt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKSALADT KEEPHVPFGE IQGYTPCGVP AYSNGHDGFF SGERSIDGNL FCGFKYQCVE FARRWLYEAK GLVLPDVNWA AHIFDLTEVH DASTATPVPC VKVSNGTAAK PVADSLLIYA VNEDAPWGHV AVITEVGDKW VRIADQNHRF HKWKGTYSAE LLLKHEGGVW TVEDHAAEGI FVPLGWVTFP SRPNRNPKEP LVLHESLYFK QPEKPFLRRV VFTPENRKTD WLDLTNEAEA EFYKTFGKEA TRGGVYESCY YLMNRELYLN CVRYGTQLHS FFLEATKQVL ESDDKLRRFR IPEEYWPRIR HSWKTQPHAI TGRFDFVFDE NTQEFKCFEY NADSASTLLE CAVIQEKWAN SVGLDDNATR SSGKFMPQTL VRAWEMTGLK GRVHFLVDDD GEERYTALYV MEKAREAGID AKLCVMFDEF HFDEKGAVVD SDGIPVTAVW KTWMWETAIS DHQAAREQRG AEWKPTPKDK VRLCDILLGN NWDIRVFEPM WKLIPSNKAI LPIIYNNHPD HPAILPASYE LTDELRRTGY AKKPIVGRVG RNVTVTEPDG KVLAESDGNF SNRDMVYQQL FRIPKRGDYY AILGGWMLGD TYSGTGVRED KKLITGLESP FGPVRIQM
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGAAGTCG GCACTTGCAG ACACTAAAGA AGAACCTCAC GTTCCCTTTG GTGAGATTCA GGGCTACACC CCTTGTGGCG TACCTGCTTA CAGCAACGGA CATGATGGCT TTTTTTCGGG AGAGCGCAGT ATTGACGGGA ATCTTTTCTG CGGTTTCAAA TACCAATGTG TAGAATTTGC TCGTCGTTGG TTATATGAGG CCAAAGGGCT TGTTCTTCCA GACGTTAATT GGGCTGCTCA CATATTTGAT CTAACAGAGG TTCATGACGC AAGCACTGCA ACCCCCGTCC CGTGCGTTAA AGTTTCCAAC GGAACAGCAG CGAAGCCCGT GGCAGATTCG TTGCTGATTT ACGCCGTTAA TGAGGATGCG CCATGGGGAC ATGTCGCGGT TATCACAGAG GTGGGTGACA AATGGGTGCG CATTGCGGAC CAAAACCATC GGTTTCATAA GTGGAAGGGG ACCTACTCGG CAGAGTTGTT GCTGAAGCAC GAAGGAGGTG TGTGGACTGT TGAGGATCAT GCAGCAGAGG GAATCTTTGT TCCTCTGGGG TGGGTTACAT TCCCTAGTAG GCCAAACCGT AATCCTAAGG AACCATTGGT GTTGCATGAG TCACTTTACT TTAAACAACC AGAGAAGCCT TTCCTTCGTC GCGTTGTTTT CACTCCAGAG AATCGAAAGA CTGACTGGCT GGACTTGACA AACGAAGCCG AAGCGGAATT CTACAAAACA TTTGGTAAGG AGGCGACTCG AGGAGGGGTG TACGAATCAT GTTACTATTT GATGAACAGG GAGTTGTACC TTAACTGCGT CCGTTACGGT ACGCAGCTGC ATTCATTCTT TCTCGAGGCA ACAAAGCAGG TGCTTGAGAG CGACGATAAG TTACGTCGAT TTCGCATCCC AGAAGAGTAT TGGCCTCGCA TCCGGCACTC ATGGAAAACT CAGCCCCATG CCATCACGGG GCGGTTTGAC TTTGTTTTTG ATGAAAACAC TCAAGAATTC AAGTGCTTTG AATACAATGC GGACAGCGCG TCGACACTTC TCGAATGTGC TGTAATCCAA GAGAAGTGGG CAAACTCCGT TGGGTTAGAC GACAATGCTA CAAGAAGCAG CGGGAAATTC ATGCCCCAAA CACTCGTCCG GGCGTGGGAA ATGACGGGGT TGAAGGGGCG TGTGCATTTT CTTGTGGACG ATGACGGGGA GGAACGTTAT ACTGCTTTGT ATGTGATGGA GAAGGCTCGA GAGGCTGGTA TTGACGCGAA ATTGTGCGTC ATGTTTGACG AGTTCCATTT CGATGAAAAG GGAGCAGTTG TGGATTCGGA CGGCATTCCG GTGACGGCTG TGTGGAAGAC GTGGATGTGG GAGACGGCGA TTTCTGATCA TCAAGCTGCA CGAGAGCAGC GTGGCGCGGA GTGGAAGCCC ACGCCAAAGG ATAAGGTTCG CCTGTGTGAT ATTCTTCTCG GAAATAACTG GGACATACGT GTCTTTGAGC CTATGTGGAA ACTCATTCCC AGCAACAAAG CAATATTGCC CATTATTTAT AATAATCACC CCGACCACCC CGCCATACTT CCCGCAAGTT ATGAACTCAC TGATGAGCTT CGCCGCACCG GCTATGCAAA GAAGCCCATC GTTGGCCGTG TAGGAAGGAA TGTGACCGTC ACTGAACCTG ACGGCAAAGT TCTAGCTGAG TCTGATGGCA ACTTCAGTAA CCGTGACATG GTGTATCAAC AACTTTTCCG CATACCAAAG CGAGGCGATT ACTACGCAAT TCTCGGGGGC TGGATGCTCG GTGACACCTA CAGTGGTACA GGTGTACGTG AGGACAAAAA GCTTATCACT GGGCTCGAAT CTCCGTTTGG TCCCGTACGT ATTCAAATGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA