SSGCID: Target Page

TrbrA.18331.b

Putative fumarate hydratase (EC 4.2.1.2) (class I, cytosolic)

CENTER ID:	TrbrA.18331.b
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.18331.b.B1.GE42089	full length		1	565

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.3.4500
UniProt:	Q580J2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLCENCELG ENCHGVDDIE ETLPITTEFH YEPIFQPSDP HHDKTEYYNI PGDYVKEIEV MGRKVLSVDP TALTVLAQHA FTDVHHYFRE DHLSGWRRVL DDPEATDNDR FVATTLLQNA CVAAGRILPA CQDTGTAIVL GKRGELCWTG GEDEKYLSHG IWKCYVSRNL RYSQTAALDM FKEANTGDNL PAQMDLMAVP GNEYHFLFVA KGGGSANKAF LYQQTKALLN PKSLRAFVEE KLKTLGTSAC PPYHIALVIG GTSAEMTMKT VKLASCRYYD SLPTTGNKSG RAFRDIEWEE IILEMTRNLG IGAQFGGKYF AHQIRVIRLP RHGASCPVGL GVSCSADRQI LGKITSEGVF LEKLVRDPAK YLPDVPLTSL GNGTVEVDLH QPMSKIRELL TQYPVTTRLS LTGPLIVARD IAHAKIMERL NADEPLPQYM KDHPIYYAGP AKTPKGMASG SFGPTTAGRM DSYVAPFMAA GGSFVTLAKG NRSKVVTDAC KKYGGFYLGS IGGPAAILAR DNIKKVEVVE YPELGMEAVW RIEVVNFPAF IIVDDKGNDF YAKLV

NT Sequence

atgagtctct gcgaaaactg tgagcttggc gagaactgcc atggtgttga cgatatcgag gaaactcttc ccattaccac agagttccat tacgaaccca tctttcaacc ctccgatcca caccatgaca agacagaata ctacaatatt cccggagatt acgtaaagga aatcgaggta atgggccgta aagtgttgag cgtcgatccc accgccttaa ccgtacttgc gcaacacgct ttcaccgacg tgcatcacta cttccgcgaa gatcatctct ccggctggcg ccgtgttctt gacgaccctg aggcaactga taatgaccgc ttcgtcgcta caacgctact gcaaaatgcc tgcgtcgctg ctggccgcat ccttcccgcc tgtcaagata cgggtactgc cattgtattg gggaagcgcg gtgagctgtg ttggacgggg ggtgaagacg agaaatatct gtcccatggc atctggaaat gctacgtgag tcgcaacctc cgctacagcc agactgctgc cttggatatg ttcaaggaag caaatacagg cgacaatctg ccggcgcaaa tggatctgat ggccgtgccc ggaaatgagt atcatttcct cttcgtcgca aagggcggtg gcagtgccaa caaggcgttt ctctaccagc aaacgaaagc tctcctaaac ccgaagtctc tccgcgcctt tgttgaagaa aaactgaaaa ctctcggcac ttcagcttgc cctccttatc acattgcact tgtcatcggg ggcaccagcg cggaaatgac gatgaaaacg gtaaaacttg cctcctgtcg ctactacgac tccctgccga caactggaaa caaaagcggc agggccttcc gcgacattga atgggaagaa atcatactgg agatgacgcg taatcttggt attggtgcgc agtttggtgg aaaatacttc gcccatcaga tccgtgtcat tcgactaccg cgccatggtg cttcatgccc tgttggtttg ggcgtctcct gcagtgcaga ccgtcaaatt ctaggaaaga taacatctga aggtgtgttc ctggagaaac tcgtgcggga cccggccaaa taccttcccg atgtgccact cacgtccctc gggaacggca ctgttgaagt ggacctccac cagccaatga gtaagattcg tgagttactt actcaatacc ccgtgacaac gcggctgagc ctcactggtc cccttatcgt ggcgcgtgac atcgcacacg caaagatcat ggaacgactt aatgccgatg agccactacc gcagtacatg aaggatcacc cgatttacta cgcggggcct gcaaagactc ccaagggaat ggcgtccggt tcctttggac ctaccaccgc tggtcgaatg gattcctacg tggcaccatt catggctgcg ggcggcagtt tcgtcaccct tgcaaaaggg aaccgctcta aagtcgttac cgacgcttgc aagaagtacg gtggatttta ccttggaagt attggcggcc ccgcggccat ccttgcgagg gacaacatca agaaggttga agttgtggaa tatccagagc tgggtatgga agccgtatgg cgcattgagg ttgtgaactt ccccgcattc attattgtgg acgacaaggg taatgacttc tatgctaaac tcgtctag

Details for TrbrA.18331.b.B1.GE42089

HARVESTED ON:	12/5/2017
SEQUENCED ON:	12/7/2017
EXPECTED MW:	63kDa
OBSERVED MW:	63kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	75
PERCENT COVERAGE:	62

Validated AA Sequence

Validated NT Sequence

ttacccttgt cgtccacaat aatgaatgcg gggaagttca caacctcaat gcgccatacg gcttccatac ccagctctgg atattccaca acttcaacct tcttgatgtt gtccctcgca aggatggccg cggggccgcc aatacttcca aggtaaaatc caccgtactt cttgcaagcg tcggtaacga ctttagagcg gttccctttt gcaagggtga cgaaactgcc gcccgcagcc atgaatggtg ccacgtagga atccattcga ccagcggtgg taggtccaaa ggaaccggac gccattccct taggagtctt tgcaggcccc gcgtagtaaa tcgggtgatc cttcatgtac tgcgggagtg gctcatcggc attaagtcgt tccatgatct ttgcgtgtgc gatgtcacgc gccacgataa ggggaccagt gaggctcagc cgcgttgtca cggggtattg agtaagtaac tcacgaatct tactcattgg ctggtggagg tccacttcaa cagtgccgtt cccgagggac gtgagtggca catcgggaag gtatttggcc gggtcccgca cgagtttctc caggaacaca ccttcagatg ttatctttcc tagaatttga cggtctgcac tgcaggagac gcccaaacca acagggcatg aagcaccatg gcgcggtagt cgaatgacac ggatctgatg ggcgaagtat tttccaccaa actgcgcacc aatacnaaga ttacgcgnca tctccagtat gatttcttcc cattcaatgt nnnnnaaggc cctgccnnnn ntgnnnnnng ttgtcggcag ggannnnnnn tagcgacagg aggcaagnnn nnnnnnnnnn nntcgtcatt nnnnnnnnnn nnnnnnnnnt gacaagtgca atgtgataag gagggcaagc nnnnnntgcc aagagttttc agtttttctt caacaaaggn nnnnngagac ttcgggttta ggagagctnt cnttnnntgg tagngnnncg ccttgttggc actgccaccg ccctttgcga cgaagaggaa atggtactca tttccgggca cggccatcag atccatttgc gccggcagat tgtcgcctgt atttgcttcc ttgaacatat ccagggcagc agtctggctg tagcggaggt tgcgactcac gtagcatttc cagatgccat gggacagata tttctcgtct tcaccccccg tccaacacag ctcaccgcgc ttccccaata caatggcagt acccgtatct tgacaggcgg gaaggatgcg gccagcagcg acgcaggcat tttgcagtag cgttgtagcg acgaagcggt cattatcagt tgcctcaggg tcgtcaagaa cacggcgcca gccggagaga tgatcttcgc ggaagtagtg atgcacgtcg gtgaaagcgt gttgcgcaag tacggttaag gcggtgggat cgacgctcaa cactttacgg cccattacct cgatttcctt tacgtaatct ccgggaatat tgtagtattc tgtcttgtca tggtgtggat cggagggttg aaagatgggt tcgtaatgga actctgtggt aatgggaaga gtttcctcga tatcgtcaac accatggcag ttctcgccaa gctcacagtt ttcgcagaga ctcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMS LCENCELGEN CHGVDDIEET LPITTEFHYE PIFQPSDPHH DKTEYYNIPG DYVKEIEVMG RKVLSVDPTA LTVLAQHAFT DVHHYFREDH LSGWRRVLDD PEATDNDRFV ATTLLQNACV AAGRILPACQ DTGTAIVLGK RGELCWTGGE DEKYLSHGIW KCYVSRNLRY SQTAALDMFK EANTGDNLPA QMDLMAVPGN EYHFLFVAKG GGSANKAFLY QQTKALLNPK SLRAFVEEKL KTLGTSACPP YHIALVIGGT SAEMTMKTVK LASCRYYDSL PTTGNKSGRA FRDIEWEEII LEMTRNLGIG AQFGGKYFAH QIRVIRLPRH GASCPVGLGV SCSADRQILG KITSEGVFLE KLVRDPAKYL PDVPLTSLGN GTVEVDLHQP MSKIRELLTQ YPVTTRLSLT GPLIVARDIA HAKIMERLNA DEPLPQYMKD HPIYYAGPAK TPKGMASGSF GPTTAGRMDS YVAPFMAAGG SFVTLAKGNR SKVVTDACKK YGGFYLGSIG GPAAILARDN IKKVEVVEYP ELGMEAVWRI EVVNFPAFII VDDKGNDFYA KLV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc tctgcgaaaa ctgtgagctt ggcgagaact gccatggtgt tgacgatatc gaggaaactc ttcccattac cacagagttc cattacgaac ccatctttca accctccgat ccacaccatg acaagacaga atactacaat attcccggag attacgtaaa ggaaatcgag gtaatgggcc gtaaagtgtt gagcgtcgat cccaccgcct taaccgtact tgcgcaacac gctttcaccg acgtgcatca ctacttccgc gaagatcatc tctccggctg gcgccgtgtt cttgacgacc ctgaggcaac tgataatgac cgcttcgtcg ctacaacgct actgcaaaat gcctgcgtcg ctgctggccg catccttccc gcctgtcaag atacgggtac tgccattgta ttggggaagc gcggtgagct gtgttggacg gggggtgaag acgagaaata tctgtcccat ggcatctgga aatgctacgt gagtcgcaac ctccgctaca gccagactgc tgccttggat atgttcaagg aagcaaatac aggcgacaat ctgccggcgc aaatggatct gatggccgtg cccggaaatg agtatcattt cctcttcgtc gcaaagggcg gtggcagtgc caacaaggcg tttctctacc agcaaacgaa agctctccta aacccgaagt ctctccgcgc ctttgttgaa gaaaaactga aaactctcgg cacttcagct tgccctcctt atcacattgc acttgtcatc gggggcacca gcgcggaaat gacgatgaaa acggtaaaac ttgcctcctg tcgctactac gactccctgc cgacaactgg aaacaaaagc ggcagggcct tccgcgacat tgaatgggaa gaaatcatac tggagatgac gcgtaatctt ggtattggtg cgcagtttgg tggaaaatac ttcgcccatc agatccgtgt cattcgacta ccgcgccatg gtgcttcatg ccctgttggt ttgggcgtct cctgcagtgc agaccgtcaa attctaggaa agataacatc tgaaggtgtg ttcctggaga aactcgtgcg ggacccggcc aaataccttc ccgatgtgcc actcacgtcc ctcgggaacg gcactgttga agtggacctc caccagccaa tgagtaagat tcgtgagtta cttactcaat accccgtgac aacgcggctg agcctcactg gtccccttat cgtggcgcgt gacatcgcac acgcaaagat catggaacga cttaatgccg atgagccact accgcagtac atgaaggatc acccgattta ctacgcgggg cctgcaaaga ctcccaaggg aatggcgtcc ggttcctttg gacctaccac cgctggtcga atggattcct acgtggcacc attcatggct gcgggcggca gtttcgtcac ccttgcaaaa gggaaccgct ctaaagtcgt taccgacgct tgcaagaagt acggtggatt ttaccttgga agtattggcg gccccgcggc catccttgcg agggacaaca tcaagaaggt tgaagttgtg gaatatccag agctgggtat ggaagccgta tggcgcattg aggttgtgaa cttccccgca ttcattattg tggacgacaa gggtaatgac ttctatgcta aactcgtctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat