TrbrA.18331.a

fumarate hydratase, putative

CENTER ID: TrbrA.18331.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.18331.a.A1.GE34302 full length 1 555
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb11.02.2700
RefSeq: XP_828580.1
UniProt: Q385R3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRRSSLWFA PKSAPPFKFV PLLPHLHHKD TEYTLVTKDY VSVVNPGAGL PEILKVEAAG LTLLASRAIG DVQHFLRPSH LASLRSIFED PEASDNDRFV ALQLIKNANI AAARILPGCQ DTGTAIIAGY KGEQVFTNGD DEEALSRGVH HIYTTTNLRY SQNVPLTMYD EKNTGCNLPA QIDLYATKGC EYEFFFVAKG GGSANKAFLF QETKSVLNPK SLRNFLEEKI STIGTSACPP YHMAVVVGGT SAEMTMKAVK YASCKYLDEL PTKPDESKGY TYRDLEMEQM VMDICRNIGM GAQFGGKYFA HDARVIRMPR HGASCPIGIG VSCSADRQAL AKINKDGIWV EKLEMDPAKY LPEVTEDQLL KVPPVKIDLN MPMDKIRAEL SRHPVKTRLS LTGTIIVARD IAHARMREML ENNKPLPDYI KNHPVYYAGP AKRPEALASG SFGPTTAGRM DPFVDLFQAN GGSFVMLAKG NRSKRVTEAC KKYGGFYLGS IGGPAALLAM DSIRKVEVLD MEELGMEAVW KIEVENFPAF IVVDDKGNDF FQQLR
NT Sequence
atgttacgac ggtcgtccct ctggtttgca cccaagtcgg cacctccctt taagtttgtc ccactgttgc ctcatcttca ccacaaagac accgagtaca cattggtaac aaaagattac gtatctgttg tcaatcccgg tgccgggtta ccggaaattc taaaggtgga agcggcagga ttaacattgc tcgcctctcg tgccattggc gatgtgcaac atttcctccg cccatcacac ctggcgagct taagaagcat ttttgaggat ccggaagcga gtgacaatga ccgttttgtt gcgctgcagc ttataaagaa tgccaatatt gcagcagctc ggattctccc tggttgccaa gacacgggca ccgcaattat tgcaggttac aagggggagc aggtgtttac caacggagat gatgaggagg cgctgagccg cggtgtgcat catatttata cgacgacgaa tttgcgctac agtcagaatg ttccgcttac catgtacgac gaaaagaaca ccggatgcaa ccttcccgca cagatagatc tctacgcaac aaagggatgt gagtacgaat tcttttttgt ggctaaaggc ggcgggagtg cgaacaaggc atttttgttc caggaaacaa aatctgttct caatcccaag tcgctgcgca attttctgga ggaaaagatt tcgacgattg gtacttccgc ttgtccacca tatcatatgg ccgtcgttgt gggaggaacc agtgctgaga tgacaatgaa ggcggtgaag tacgcttcct gcaagtatct ggatgaactc ccaacgaagc ctgatgaatc gaagggttat acctatcgcg atctggaaat ggaacaaatg gttatggata tttgccgcaa tattggcatg ggcgcacagt ttggtgggaa gtattttgcc cacgatgcac gcgtcatccg catgccacgt cacggtgcca gctgccccat cggaattggc gttagctgca gtgcggaccg ccaagcactt gctaaaatta acaaagatgg catttgggtc gagaaacttg aaatggaccc agcgaagtat ctgccagagg taacggagga tcaattactt aaggtgccac cagtgaagat cgacttgaac atgcccatgg acaaaatacg tgcagagctc tcgagacacc ccgtgaagac gcgcctgtct ctcactggaa ccatcatcgt agcccgtgac atcgcgcatg cccgtatgcg ggaaatgttg gagaacaata aaccccttcc agattacatc aagaaccacc cggtatatta tgccggtcct gccaagcgac cagaggcgtt ggcatccggt tcctttgggc caaccacagc gggccgtatg gacccgtttg tggatctctt ccaggcaaat ggcgggtctt tcgtcatgtt ggctaaaggc aaccgtagca aacgtgtgac tgaggcatgt aagaagtacg gtgggtttta ccttggaagt attgggggac ctgccgcgtt gctagcaatg gattccattc gcaaagtgga agtacttgac atggaggaac tgggaatgga ggcggtatgg aagattgaag ttgagaactt tcccgcattt attgttgtgg acgataaggg taatgacttc ttccaacagc tcaggtag
Details for TrbrA.18331.a.A1.GE34302
HARVESTED ON: 12/19/2011
SEQUENCED ON: 1/3/2012
EXPECTED MW: 63kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 35
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLRRSSLWF APKSAPPFKF VPLLPHLHHK DTEYTLVTKD YVSVVNPGAG LPEILKVEAA GLTLLASRAI GDVQHFLRPS HLASLRSIFE DPEASDNDRF VALQLIKNAN IAAARILPGC QDTGTAIIAG YKGEQVFTNG DDEEALSRGV HHIYTTTXLR YSQNVPLTMY DERTPXXTXP HR
Validated NT Sequence
ttttgnttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttac gacggtcgtc cctctggttt gcacccaagt cggcacctcc ttttaagttt gtcccactgt tgcctcatct tcaccacaaa gacaccgagt acacattggt aacaaaggat tacgtatctg ttgtcaatcc cggtgccggg ttaccggaaa ttctaaaggt ggaagcggca ggattaacat tgctcgcctc tcgtgccatt ggcgatgtgc aacatttcct ccgcccatca cacctggcga gcttaagaag catttttgaa gatcccgaag cgagtgacaa tgaccgtttt gttgcgctgc agcttataaa gaatgccaat attgcagcag ctcggattct ccctggntgc caagacacgg gcaccgcaat tattgcaggt tacaaggggg agcaggtgtt taccaacgga gatgatgagg aggcgctgag ccgcggtgtg catcatattt atacgacgac gaanttgcgc tacagtcaga atgttccgct taccatgtac gacgaaagaa caccggangn aaccntcccg cacagataga tctctacgca acnnnggatg tgagtncnan ttcttttttg tggctnaagg cggcgggagt gcgaacaagg catttttgnt ccangnaaca aaatctgttc tcaatccnna gtngctgcgc anntttctgg angaaanatt tcnacgatng nnacttccgc tngncnnncn natcnnngnn nncntntggg annannnagt gctgannnga nnnnanncng nnantangcn ncnnnagtnt cnngannann nccnncnanc cnnnngancn angntnnnnc cnnnnnnnnn cnnnntnnnn nnaatnntnn nnnnnnnncn gnannnnnnn nngttnnnnn ggnnnnnnnn nnnnnannnn nnnnnntcnn nnncnnnnnn nncccnnnnn nnnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRRSSLWF APKSAPPFKF VPLLPHLHHK DTEYTLVTKD YVSVVNPGAG LPEILKVEAA GLTLLASRAI GDVQHFLRPS HLASLRSIFE DPEASDNDRF VALQLIKNAN IAAARILPGC QDTGTAIIAG YKGEQVFTNG DDEEALSRGV HHIYTTTNLR YSQNVPLTMY DEKNTGCNLP AQIDLYATKG CEYEFFFVAK GGGSANKAFL FQETKSVLNP KSLRNFLEEK ISTIGTSACP PYHMAVVVGG TSAEMTMKAV KYASCKYLDE LPTKPDESKG YTYRDLEMEQ MVMDICRNIG MGAQFGGKYF AHDARVIRMP RHGASCPIGI GVSCSADRQA LAKINKDGIW VEKLEMDPAK YLPEVTEDQL LKVPPVKIDL NMPMDKIRAE LSRHPVKTRL SLTGTIIVAR DIAHARMREM LENNKPLPDY IKNHPVYYAG PAKRPEALAS GSFGPTTAGR MDPFVDLFQA NGGSFVMLAK GNRSKRVTEA CKKYGGFYLG SIGGPAALLA MDSIRKVEVL DMEELGMEAV WKIEVENFPA FIVVDDKGND FFQQLR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttacgacgg tcgtccctct ggtttgcacc caagtcggca cctcccttta agtttgtccc actgttgcct catcttcacc acaaagacac cgagtacaca ttggtaacaa aagattacgt atctgttgtc aatcccggtg ccgggttacc ggaaattcta aaggtggaag cggcaggatt aacattgctc gcctctcgtg ccattggcga tgtgcaacat ttcctccgcc catcacacct ggcgagctta agaagcattt ttgaggatcc ggaagcgagt gacaatgacc gttttgttgc gctgcagctt ataaagaatg ccaatattgc agcagctcgg attctccctg gttgccaaga cacgggcacc gcaattattg caggttacaa gggggagcag gtgtttacca acggagatga tgaggaggcg ctgagccgcg gtgtgcatca tatttatacg acgacgaatt tgcgctacag tcagaatgtt ccgcttacca tgtacgacga aaagaacacc ggatgcaacc ttcccgcaca gatagatctc tacgcaacaa agggatgtga gtacgaattc ttttttgtgg ctaaaggcgg cgggagtgcg aacaaggcat ttttgttcca ggaaacaaaa tctgttctca atcccaagtc gctgcgcaat tttctggagg aaaagatttc gacgattggt acttccgctt gtccaccata tcatatggcc gtcgttgtgg gaggaaccag tgctgagatg acaatgaagg cggtgaagta cgcttcctgc aagtatctgg atgaactccc aacgaagcct gatgaatcga agggttatac ctatcgcgat ctggaaatgg aacaaatggt tatggatatt tgccgcaata ttggcatggg cgcacagttt ggtgggaagt attttgccca cgatgcacgc gtcatccgca tgccacgtca cggtgccagc tgccccatcg gaattggcgt tagctgcagt gcggaccgcc aagcacttgc taaaattaac aaagatggca tttgggtcga gaaacttgaa atggacccag cgaagtatct gccagaggta acggaggatc aattacttaa ggtgccacca gtgaagatcg acttgaacat gcccatggac aaaatacgtg cagagctctc gagacacccc gtgaagacgc gcctgtctct cactggaacc atcatcgtag cccgtgacat cgcgcatgcc cgtatgcggg aaatgttgga gaacaataaa ccccttccag attacatcaa gaaccacccg gtatattatg ccggtcctgc caagcgacca gaggcgttgg catccggttc ctttgggcca accacagcgg gccgtatgga cccgtttgtg gatctcttcc aggcaaatgg cgggtctttc gtcatgttgg ctaaaggcaa ccgtagcaaa cgtgtgactg aggcatgtaa gaagtacggt gggttttacc ttggaagtat tgggggacct gccgcgttgc tagcaatgga ttccattcgc aaagtggaag tacttgacat ggaggaactg ggaatggagg cggtatggaa gattgaagtt gagaactttc ccgcatttat tgttgtggac gataagggta atgacttctt ccaacagctc aggtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa