TrbrA.18281.a

V-type ATPase, A subunit, putative

CENTER ID: TrbrA.18281.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.18281.a.A1.GE34292 full length 1 610
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.4.1080
RefSeq: XP_844258.1
UniProt: Q580D6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSDRNPYKT EQRMGSVKAV SGPVVIAENM GGSAMYELVQ VGSFRLVGEI IRLEGDTATI QVYEETGGLT VGDPVYCTGK PLSLELGPGI MSEIFDGIQR PLDTIYRMVE NVFIPRGVQV KSLNDQKQWD FKPCLKVGDL ISGGDIIGSV VENSLMYNHS IMVPPNIRGR VTSIVASGNY TLEDDIVELE YNGKVKSLKL MHRWPVRTPR PVASKESGNH PLLTGQRVLD ALFPSVQGGT CAIPGAFGCG KTVISQALSK YSNSDAVIYV GCGERGNEMA EVLMDFPTLT TIIDGREESI MKRTCLVANT SNMPVAAREA SIYTGITLAE YYRDMGKHIA MMADSTSRWA EALREISGRL AEMPADGGYP AYLSARLASF YERAGRVTCI GGPKREGSVT IVGAVSPPGG DFSDPVTSAT LGIVQVFWGL EKRLAQRKHF PSVNWLISYS KYLKALEPFF NSLDPDYMRL RSVAAEILQR EEELQEIVQL VGKDSLSESD KIILETAKVI REEFLQQNAF TPYDKYCPPY KTCWMLRNIV TFYEEGQRVV AESAGEHKIT WNYIREKIPH VYTGLTEMKF RDPQEGEEAN VKYYKKQHEE IMTAFASLVQ
NT Sequence
atgtcgagcg accgcaaccc atacaaaaca gagcagcgga tgggttccgt caaggctgtt tccggacctg tcgttattgc ggagaacatg ggcggcagcg cgatgtacga acttgtgcag gtgggctcat ttcggttggt gggtgaaatc attcgactag agggtgatac ggccactatt caagtgtacg aggaaactgg cgggctaacc gttggagatc cagtctactg tacaggtaag cctctctccc ttgaacttgg gccgggtatc atgtcagaaa tatttgatgg gattcagcgc cccctcgata ccatctaccg catggtggag aacgtcttca ttcctagggg cgtccaggtg aagtccctca acgaccagaa acaatgggat tttaaaccat gcttgaaggt tggggacctc atttccggtg gcgatattat tggttcggtc gtggagaact cactgatgta caaccacagt ataatggtcc cgccaaacat ccgagggagg gtcacttcca ttgtggcttc tggaaactac acactggagg acgatattgt ggagttggag tacaatggga aggtcaagtc gctcaagctc atgcaccggt ggccggtgcg gacccccagg cctgtggctt caaaggagtc tggaaaccac ccacttctta ctggtcagcg cgtgcttgat gccctgtttc catccgtgca gggcggaacc tgtgccatcc ccggtgcgtt tggctgtggc aagacagtta tcagtcaggc actctcgaag tactcgaaca gtgacgctgt tatttacgtg ggttgtggtg agcgtggcaa tgagatggcg gaggtgctca tggactttcc cactctcacc accattattg atggacgtga ggagtctatc atgaagcgca catgcctggt ggcaaatact tccaatatgc ctgttgctgc tcgtgaggca tctatttaca ctggtatcac cttagctgag tattatcggg atatgggaaa acacatcgcc atgatggccg attcaacctc ccgctgggct gaggctttgc gtgagatttc cggtcgtctt gctgaaatgc ctgcagatgg aggttatccc gcgtacctca gcgcccgttt ggcctccttc tacgagcgtg ctggccgtgt gacatgcatc ggtgggccca agcgtgaagg gtctgtaaca attgttggtg ccgtttcccc accgggtggt gatttctccg atcccgtaac gtccgccacc cttggtatcg tgcaagtttt ctggggactc gagaagcgac tcgctcaacg caaacacttc ccgtccgtga actggctcat ttcttactcc aagtacctca aggctttgga gcccttcttc aattctctcg accccgacta catgcggctg cgctcagttg ccgctgagat tttgcagcgc gaagaggagc tgcaggaaat tgttcagctc gtcggcaaag actccctttc tgagtctgac aagataattc tagagactgc gaaagttatc cgtgaggaat ttcttcagca gaatgccttc actccgtatg acaagtactg cccaccgtat aagacatgct ggatgttgcg caacattgtc accttctacg aggagggtca gcgagttgta gcggagtctg cgggcgagca caagatcacg tggaattaca ttcgcgaaaa gatccctcat gtctacactg gtctcacgga aatgaagttc cgagaccctc aggaaggaga agaagccaac gttaagtact acaagaagca acacgaggag atcatgaccg cattcgcttc cctcgtgcag tag
Details for TrbrA.18281.a.A1.GE34292
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 70kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 49
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSDRNPYK TEQRMGSVKA VSGPVVIAEN MGGSAMYELV QVGSFRLVGE IIRLEGDTAT IQVYEETGGL TVGDPVYCTG KPLSLELGPG IMSEIFDGIQ RPLDTIYRMV ENVFIPRGVQ VKSLNDQKQW DFKPCLKVGD LISGGDIIGS VVENSLMYNH SIMVPPNIRG RVTSIVASGN YTLEDDIVEL EYNGKVKSLK LMHRWPVRTP RPVASKESGN HPLLTGQRVL DALFPSVQGG TCAIPGAFGC GKTVISQALS KYSNSDAVIY VGXGERGNEM AXXSWXFXXX HHX
Validated NT Sequence
annattttnt ttactttaag aaggaganat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgt cgagcgaccg caacccatac aaaacagagc agcggatggg ttccgtcaag gctgtttccg gacctgtcgt tattgcggag aacatgggcg gcagcgcgat gtacgaactt gtgcaggtgg gctcatttcg gttggtgggt gaaatcattc gactagaggg tgatacggcc actattcaag tgtacgagga aactggcggg ctaaccgttg gagatccagt ctactgtaca ggtaagcctc tctcccttga acttgggccg ggtatcatgt cagaaatatt tgatgggatt cagcgccccc tcgataccat ctaccgcatg gtggagaacg tcttcattcc taggggcgtt caggtgaagt ccctcaacga ccagaaacaa tgggatttta aaccatgctt gaaggttggg gacctcattt ccggtggcga tattattggt tcggtcgtgg agaactcact gatgtacaac cacagtataa tggtcccgcc aaacatccga gggagggtca cttccattgt ggcttctgga aactacacac tggaggacga tattgtggag ttggagtaca atgggaaggt caagtcgctc aagctcatgc accggtggcc ggtgcggacc cccaggcctg tggcttcaaa ggagtccgga aaccacccac ttcttactgg tcagcgcgtg cttgatgccc tgtttccatc cgtgcagggc gggacctgtg ccatccccgg tgcgtttggc tgtggcaaga cagttatcag tcaggcactc tcgaagtact cgaacagtga cgctgttatt tacgtgggtt gnggtgagcg tggcaatgag atggcgnnnn gctcatggnc tttccnancn ncncaccatt antgatggac gtgagnagtc tatcatgaag cnnacntgcc tgnngnnnna ctnnnnatct nntcnngnng catctantta cnctngnntn nncnnanctg annnnnncgn nnnnnnnaan nnnntnnncn tgnatgnnnn annnnnnnnn ccnnntggnn nnnnnnnntt tnnnnnnnnn nannt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSDRNPYK TEQRMGSVKA VSGPVVIAEN MGGSAMYELV QVGSFRLVGE IIRLEGDTAT IQVYEETGGL TVGDPVYCTG KPLSLELGPG IMSEIFDGIQ RPLDTIYRMV ENVFIPRGVQ VKSLNDQKQW DFKPCLKVGD LISGGDIIGS VVENSLMYNH SIMVPPNIRG RVTSIVASGN YTLEDDIVEL EYNGKVKSLK LMHRWPVRTP RPVASKESGN HPLLTGQRVL DALFPSVQGG TCAIPGAFGC GKTVISQALS KYSNSDAVIY VGCGERGNEM AEVLMDFPTL TTIIDGREES IMKRTCLVAN TSNMPVAARE ASIYTGITLA EYYRDMGKHI AMMADSTSRW AEALREISGR LAEMPADGGY PAYLSARLAS FYERAGRVTC IGGPKREGSV TIVGAVSPPG GDFSDPVTSA TLGIVQVFWG LEKRLAQRKH FPSVNWLISY SKYLKALEPF FNSLDPDYMR LRSVAAEILQ REEELQEIVQ LVGKDSLSES DKIILETAKV IREEFLQQNA FTPYDKYCPP YKTCWMLRNI VTFYEEGQRV VAESAGEHKI TWNYIREKIP HVYTGLTEMK FRDPQEGEEA NVKYYKKQHE EIMTAFASLV Q
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgagcgac cgcaacccat acaaaacaga gcagcggatg ggttccgtca aggctgtttc cggacctgtc gttattgcgg agaacatggg cggcagcgcg atgtacgaac ttgtgcaggt gggctcattt cggttggtgg gtgaaatcat tcgactagag ggtgatacgg ccactattca agtgtacgag gaaactggcg ggctaaccgt tggagatcca gtctactgta caggtaagcc tctctccctt gaacttgggc cgggtatcat gtcagaaata tttgatggga ttcagcgccc cctcgatacc atctaccgca tggtggagaa cgtcttcatt cctaggggcg tccaggtgaa gtccctcaac gaccagaaac aatgggattt taaaccatgc ttgaaggttg gggacctcat ttccggtggc gatattattg gttcggtcgt ggagaactca ctgatgtaca accacagtat aatggtcccg ccaaacatcc gagggagggt cacttccatt gtggcttctg gaaactacac actggaggac gatattgtgg agttggagta caatgggaag gtcaagtcgc tcaagctcat gcaccggtgg ccggtgcgga cccccaggcc tgtggcttca aaggagtctg gaaaccaccc acttcttact ggtcagcgcg tgcttgatgc cctgtttcca tccgtgcagg gcggaacctg tgccatcccc ggtgcgtttg gctgtggcaa gacagttatc agtcaggcac tctcgaagta ctcgaacagt gacgctgtta tttacgtggg ttgtggtgag cgtggcaatg agatggcgga ggtgctcatg gactttccca ctctcaccac cattattgat ggacgtgagg agtctatcat gaagcgcaca tgcctggtgg caaatacttc caatatgcct gttgctgctc gtgaggcatc tatttacact ggtatcacct tagctgagta ttatcgggat atgggaaaac acatcgccat gatggccgat tcaacctccc gctgggctga ggctttgcgt gagatttccg gtcgtcttgc tgaaatgcct gcagatggag gttatcccgc gtacctcagc gcccgtttgg cctccttcta cgagcgtgct ggccgtgtga catgcatcgg tgggcccaag cgtgaagggt ctgtaacaat tgttggtgcc gtttccccac cgggtggtga tttctccgat cccgtaacgt ccgccaccct tggtatcgtg caagttttct ggggactcga gaagcgactc gctcaacgca aacacttccc gtccgtgaac tggctcattt cttactccaa gtacctcaag gctttggagc ccttcttcaa ttctctcgac cccgactaca tgcggctgcg ctcagttgcc gctgagattt tgcagcgcga agaggagctg caggaaattg ttcagctcgt cggcaaagac tccctttctg agtctgacaa gataattcta gagactgcga aagttatccg tgaggaattt cttcagcaga atgccttcac tccgtatgac aagtactgcc caccgtataa gacatgctgg atgttgcgca acattgtcac cttctacgag gagggtcagc gagttgtagc ggagtctgcg ggcgagcaca agatcacgtg gaattacatt cgcgaaaaga tccctcatgt ctacactggt ctcacggaaa tgaagttccg agaccctcag gaaggagaag aagccaacgt taagtactac aagaagcaac acgaggagat catgaccgca ttcgcttccc tcgtgcagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a