SSGCID: Target Page

TrbrA.18272.a

peptidylprolyl isomerase-like protein

CENTER ID:	TrbrA.18272.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.18272.a.A1.GE34296	full length		1	425

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.18272.a.A1.PW34904	full length		1	425

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.10.16100

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSPESSVTS LDSQPVREVE YPVGVQTEVP DTNGGLFKTV LIEGSGTKPI KGSKVTVHYV GTLESDGSKF DSSRDRGEYF EFTLGRGQVI KGWDRGVATM RVGEKAVLRC TPEYGYGAAG SPPKIPANAT LLFEVELFSW TREEDISESK DKSIMKSLAV EGIDYEKPGY ESKLKVDLRV YAGPHSDDQP GTLLCERLDW ELTLGDTPLP PHLETCLSTM RKRESASFRI DPRLSTEHNE EFNISPGSQL TYAVELRELT TVKTWMFEGP ARVEEAERRR AQGNEAVRSG KFSVAERKYR RALEFVEADS GFGSDNDESL ASARKVRLVL WGNLAQALLA QGSHQECIRY CNRVLEVEPG NAKALFRRAK AYDAQSDWHE AKGDLETILQ ADPQNTDARV LLQRVQAQRK AYEKKQREAY KKMFS

NT Sequence

atgagctcac cagagagcag tgtgacgagc ttggacagtc aacctgttcg cgaggtggag taccccgtcg gtgtgcaaac cgaggttccg gacacgaatg gcggtctctt caagacggtg cttattgagg gaagcgggac taagccaata aagggttcga aggtgacagt acactacgtc ggcacgctgg agtccgatgg ctccaagttc gacagcagtc gtgaccgcgg ggaatacttc gagttcacgt tgggtcgcgg gcaggtcatc aaaggttggg atcggggcgt cgccactatg cgggtggggg agaaggcagt tttgcgctgc acaccagaat acggctacgg tgcggccggt agcccgccaa agataccagc gaacgccaca cttctatttg aagtggagct attcagttgg acacgggagg aggatatttc cgagagcaag gacaaatcga tcatgaaaag ccttgctgtc gagggtatcg attacgaaaa gcctggatac gaatccaagc tgaaagttga cctacgcgtg tacgcggggc cgcacagcga tgatcagcca gggacgctgc tgtgcgagcg gctcgactgg gaactcacct tgggtgacac gccgctgcca ccccaccttg agacatgcct ctcaactatg cggaagcgcg aatctgcttc cttccgtatc gatccccgtc tttccacgga acacaacgag gagttcaaca tatcccctgg ctcgcagcta acatacgcag tagagttgcg tgagctcact acggtcaaaa catggatgtt cgagggtccg gcacgggtgg aagaagcaga gcgacggagg gcacagggca atgaggccgt aaggagcgga aagttcagcg tcgcagaacg gaaatatcgt cgagcactgg aatttgtcga ggcagatagc ggtttcggca gtgataatga tgagagcttg gcgagtgccc gcaaagtccg tctggtgctt tggggtaacc tggcgcaggc ccttcttgcc caagggtctc accaggagtg tatccggtac tgtaacaggg tgctggaggt tgaaccaggt aacgcaaagg cattgttccg ccgggcaaag gcctatgatg cacagagtga ctggcacgag gcgaaggggg atcttgagac gatcctgcaa gcagatccac agaatacgga cgccagggtg ctgttgcagc gggtacaggc acaacgcaaa gcctacgaga aaaagcagcg tgaggcatat aagaagatgt tttcgtag

Details for TrbrA.18272.a.A1.GE34296

HARVESTED ON:	12/19/2011
SEQUENCED ON:	12/28/2011
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	60

Validated AA Sequence

MKSLAVEGXD YEKPGXESKL KVDXRVYAGP HSDDQPGTLL CERLDWELTL GDTPLPPHLE TCLSTMRKRE SASFRIDPRL STEHNEEFNI SPGSQLTYAV ELRELTTVKT WMFEGPARVE EAERRRAQGN EAVRSGKFSV AERKYRRALE FVEADSGFGS DNDESLASAR KVRLVLWGNL AQALLAQGSH QECIRYCNRV LEVEPGNAKA LFRRAKAYDA QSDWHEAKGD LETILQADPQ NTDARVLLQR VQAQRKAYEK KQREAYKKM

Validated NT Sequence

aaacatcttc ttatatgcct cacgctgctt tttctcgtag gctttgcgtt gtgcctgtac ccgctgcaac agcaccctgg cgtccgtatt ctgtggatct gcttgcagga tcgtctcaag atcccccttc gcctcgtgcc agtcactctg tgcatcatag gcctttgccc ggcggaacaa tgcctttgcg ttacctggtt caacctccag caccctgtta cagtaccgga tacactcctg gtgagaccct tgggcaagaa gggcctgcgc caggttaccc caaagcacca gacggacttt gcgggcactc gccaagctct catcattatc actgccgaaa ccgctatctg cctcgacaaa ttccagtgct cgacgatatt tccgttctgc gacgctgaac tttccgctcc ttacggcctc attgccctgt gccctccgtc gctctgcttc ttccacccgt gccggaccct cgaacatcca tgttttgacc gtagtgagct cgcgcaactc tactgcgtat gttagctgcg agccagggga tatgttgaac tcctcgttgt gttccgtgga aagacgggga tcgatacgga aggaagcaga ttcgcgcttc cgcatagttg agaggcatgt ctcaaggtgg ggtggcagcg gcgtgtcacc caaggtgagt tcccagtcga gccgctcgca cagcagcgtc cctggctggt catcgctgtg cggccccgcg tacacgcgta ngtcaacttt cagcttggat tcntatccng gcttttcgta atcnataccc tcgacagcaa ggcttttcat gatcgatttg tccttgctct cggaaatatc ctcctcccgn gtccnnctga atanctncnc ttcaannaga agtgtggcgt tcgctggtat cttcggcggg ctgccg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSSPESSVT SLDSQPVREV EYPVGVQTEV PDTNGGLFKT VLIEGSGTKP IKGSKVTVHY VGTLESDGSK FDSSRDRGEY FEFTLGRGQV IKGWDRGVAT MRVGEKAVLR CTPEYGYGAA GSPPKIPANA TLLFEVELFS WTREEDISES KDKSIMKSLA VEGIDYEKPG YESKLKVDLR VYAGPHSDDQ PGTLLCERLD WELTLGDTPL PPHLETCLST MRKRESASFR IDPRLSTEHN EEFNISPGSQ LTYAVELREL TTVKTWMFEG PARVEEAERR RAQGNEAVRS GKFSVAERKY RRALEFVEAD SGFGSDNDES LASARKVRLV LWGNLAQALL AQGSHQECIR YCNRVLEVEP GNAKALFRRA KAYDAQSDWH EAKGDLETIL QADPQNTDAR VLLQRVQAQR KAYEKKQREA YKKMFS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagctcacca gagagcagtg tgacgagctt ggacagtcaa cctgttcgcg aggtggagta ccccgtcggt gtgcaaaccg aggttccgga cacgaatggc ggtctcttca agacggtgct tattgaggga agcgggacta agccaataaa gggttcgaag gtgacagtac actacgtcgg cacgctggag tccgatggct ccaagttcga cagcagtcgt gaccgcgggg aatacttcga gttcacgttg ggtcgcgggc aggtcatcaa aggttgggat cggggcgtcg ccactatgcg ggtgggggag aaggcagttt tgcgctgcac accagaatac ggctacggtg cggccggtag cccgccaaag ataccagcga acgccacact tctatttgaa gtggagctat tcagttggac acgggaggag gatatttccg agagcaagga caaatcgatc atgaaaagcc ttgctgtcga gggtatcgat tacgaaaagc ctggatacga atccaagctg aaagttgacc tacgcgtgta cgcggggccg cacagcgatg atcagccagg gacgctgctg tgcgagcggc tcgactggga actcaccttg ggtgacacgc cgctgccacc ccaccttgag acatgcctct caactatgcg gaagcgcgaa tctgcttcct tccgtatcga tccccgtctt tccacggaac acaacgagga gttcaacata tcccctggct cgcagctaac atacgcagta gagttgcgtg agctcactac ggtcaaaaca tggatgttcg agggtccggc acgggtggaa gaagcagagc gacggagggc acagggcaat gaggccgtaa ggagcggaaa gttcagcgtc gcagaacgga aatatcgtcg agcactggaa tttgtcgagg cagatagcgg tttcggcagt gataatgatg agagcttggc gagtgcccgc aaagtccgtc tggtgctttg gggtaacctg gcgcaggccc ttcttgccca agggtctcac caggagtgta tccggtactg taacagggtg ctggaggttg aaccaggtaa cgcaaaggca ttgttccgcc gggcaaaggc ctatgatgca cagagtgact ggcacgaggc gaagggggat cttgagacga tcctgcaagc agatccacag aatacggacg ccagggtgct gttgcagcgg gtacaggcac aacgcaaagc ctacgagaaa aagcagcgtg aggcatataa gaagatgttt tcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for TrbrA.18272.a.A1.PW34904

PURIFICATION DATe:	2/3/2012
CONCENTRATION:	24.3mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	110µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	60

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCTCACCA GAGAGCAGTG TGACGAGCTT GGACAGTCAA CCTGTTCGCG AGGTGGAGTA CCCCGTCGGT GTGCAAACCG AGGTTCCGGA CACGAATGGC GGTCTCTTCA AGACGGTGCT TATTGAGGGA AGCGGGACTA AGCCAATAAA GGGTTCGAAG GTGACAGTAC ACTACGTCGG CACGCTGGAG TCCGATGGCT CCAAGTTCGA CAGCAGTCGT GACCGCGGGG AATACTTCGA GTTCACGTTG GGTCGCGGGC AGGTCATCAA AGGTTGGGAT CGGGGCGTCG CCACTATGCG GGTGGGGGAG AAGGCAGTTT TGCGCTGCAC ACCAGAATAC GGCTACGGTG CGGCCGGTAG CCCGCCAAAG ATACCAGCGA ACGCCACACT TCTATTTGAA GTGGAGCTAT TCAGTTGGAC ACGGGAGGAG GATATTTCCG AGAGCAAGGA CAAATCGATC ATGAAAAGCC TTGCTGTCGA GGGTATCGAT TACGAAAAGC CTGGATACGA ATCCAAGCTG AAAGTTGACC TACGCGTGTA CGCGGGGCCG CACAGCGATG ATCAGCCAGG GACGCTGCTG TGCGAGCGGC TCGACTGGGA ACTCACCTTG GGTGACACGC CGCTGCCACC CCACCTTGAG ACATGCCTCT CAACTATGCG GAAGCGCGAA TCTGCTTCCT TCCGTATCGA TCCCCGTCTT TCCACGGAAC ACAACGAGGA GTTCAACATA TCCCCTGGCT CGCAGCTAAC ATACGCAGTA GAGTTGCGTG AGCTCACTAC GGTCAAAACA TGGATGTTCG AGGGTCCGGC ACGGGTGGAA GAAGCAGAGC GACGGAGGGC ACAGGGCAAT GAGGCCGTAA GGAGCGGAAA GTTCAGCGTC GCAGAACGGA AATATCGTCG AGCACTGGAA TTTGTCGAGG CAGATAGCGG TTTCGGCAGT GATAATGATG AGAGCTTGGC GAGTGCCCGC AAAGTCCGTC TGGTGCTTTG GGGTAACCTG GCGCAGGCCC TTCTTGCCCA AGGGTCTCAC CAGGAGTGTA TCCGGTACTG TAACAGGGTG CTGGAGGTTG AACCAGGTAA CGCAAAGGCA TTGTTCCGCC GGGCAAAGGC CTATGATGCA CAGAGTGACT GGCACGAGGC GAAGGGGGAT CTTGAGACGA TCCTGCAAGC AGATCCACAG AATACGGACG CCAGGGTGCT GTTGCAGCGG GTACAGGCAC AACGCAAAGC CTACGAGAAA AAGCAGCGTG AGGCATATAA GAAGATGTTT TCGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA