SSGCID: Target Page

TrbrA.18248.b

hypothetical protein, conserved

CENTER ID:	TrbrA.18248.b
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.18248.b.A1.GE34303	full length		1	381

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.18248.b.A1.PS01475	full length		1	381

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.3.1060

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

LGIELAPCVD IKPILASDEK KPIIFFPPPP NGFGSERENR SSWLTLNPRP MRRDVEKIEK EEGRVMNFLA ELANPLVRGD EKRRFVISFF RETDEMSIYE KPERNSGYLA GRFLAKGVYR KPMPDGSTVP YTAEDFQVGK EITILERPFR LLDMSEETKR ILTVTEQLPS EQRLKELLLL FKQQIQLKFT RGHEAYCTLA PKGVLGYRQV REFLRSCSCS ITEDEALLLV HNLVPSSAGV ISFNEFMDLV NITSSEHMDE ASLTVRSVKS VNMTKDESLK TVAIKTEDVK RRKQLAVELR QKLIQRKGSV QEQFRLIGCH SASSRLNRDV FRHSLNEVMH FNVPKTDEDM LVSLLFDGRA DENGDITYKQ FQEFLEVQDD I

NT Sequence

cttggtattg aactcgcacc gtgtgtagac ataaagccta tccttgcctc cgacgagaaa aagccaataa tattctttcc tcccccacca aacggctttg ggagcgaacg agaaaaccga tcaagttggc taactttaaa tccaaggccg atgcgccgtg atgttgaaaa aattgaaaaa gaagagggca gggtcatgaa ttttttggcg gagttggcca atcctttggt ccgcggtgat gagaagagga gatttgttat atcctttttt agagaaactg atgaaatgtc tatttatgaa aaacccgaac ggaactcagg ttaccttgcc gggaggtttc ttgcaaaagg tgtttatcgg aaaccaatgc cagatggctc aaccgttccc tacacggcag aggacttcca ggttgggaag gagattacaa tacttgagcg tccttttcgt ctcctcgata tgagtgaaga gacgaagagg atacttaccg taacggagca actcccttct gagcagcggc tgaaggagtt acttctttta ttcaagcagc aaattcagct aaaatttact cgtggccacg aagcctactg tacattggct cctaagggag tattgggcta tcgtcaggtt cgcgaattct taaggtcgtg tagctgttct ataaccgagg acgaagcact cctcctcgta cacaacctcg ttccttctag tgccggagtg atttcattta atgagttcat ggatttggtg aacataacct cttcagaaca tatggatgaa gcttcactga cggtgcgctc tgtaaagagc gtaaacatga caaaagacga atcactgaag acagtagcta tcaaaacaga ggacgtgaaa cgaaggaaac agcttgcagt tgagttacgc caaaagttga tccaaaggaa gggttccgta caggagcagt ttcgcctcat tggttgccac agcgcatcat cccggttaaa tcgagatgtt ttccgccact cactgaacga agtaatgcac tttaacgttc ctaagacgga cgaggatatg cttgtgtcac tccttttcga tgggagagct gacgaaaacg gcgacataac gtacaagcaa ttccaagaat tccttgaggt tcaggatgat atctag

Details for TrbrA.18248.b.A1.GE34303

HARVESTED ON:	12/19/2011
SEQUENCED ON:	1/3/2012
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Validated AA Sequence

MLGIELAPCV DIKPILASDE KKPIIFFPPP PNGFGSEREN RSSWLTLNPR PMRRDVEKIE KEEGRVMNFL AELANPLVRG DEKRRFVISF FRETDEMSIF EKPERNSGYL AGRFLAKGVY RKPMPDGSTV PYTAEDFQVG KEITILERPF RLLDMSEETK RILTVTEQLP SEQRLKELLL LFKQQIQLKF TRGHEAYCTL APKGVLGYRQ VREFLRSCSC SITEDEALLL VHNLVPSSAG VISFNEFMDL VNITSSEHMD EASLTVRSVK SVNMTKDESL KTVAIKTEDV KRRKQLAVEL RQKLIQRKGS VQEQFRLIGC HSASSRLNRD VFRHSLNEVM HFNVPKTDED MLVSLLFDGR ADENGDITYK QFQEFLEVQD

Validated NT Sequence

catcctgaac ctcaaggaat tcttggaatt gcttgtacgt tatgtcgccg ttttcgtcag ctctcccatc gaaaaggagt gacacaagca tatcctcgtc cgtcttagga acgttaaagt gcattacttc gttcagtgag tggcggaaaa catctcgatt taaccgggat gatgcgctgt ggcaaccaat gaggcgaaac tgctcctgta cggaaccctt cctttggatc aacttttggc gtaactcaac tgcaagctgt ttccttcgtt tcacgtcctc tgttttgata gctactgttt tcagtgattc gtcttttgtc atgtttacgc tctttacaga gcgcaccgtc agtgaagctt catccatatg ttctgaagag gttatgttca ccaaatccat gaactcatta aatgaaatca ctccggcact agaaggaacg aggttgtgta cgaggaggag tgcttcgtcc tcggttatag aacagctaca cgaccttaag aattcgcgaa cctgacgata gcccaatact cccttaggag ccaatgtaca gtaggcttcg tggccacgag taaattttag ctgaatttgc tgcttgaata aaagaagtaa ctccttcagc cgctgctcag aagggagttg ctccgttacg gtaagtatcc tcttcgtctc ttcactcata tcgaggagac gaaaaggacg ctcaagtatt gtaatctcct tcccaacctg gaagtcctct gccgtatagg gaacagttga gccatctggc attggtttcc gataaacacc ttttgcaaga aacctcccgg caaggtaacc tgagttccgt tcgggttttt caaaaataga catttcatca gtttctctaa aaaaggatat aacaaatctc ctcttctcat caccgcggac caaaggattg gccaactccg ccaaaaaatt catgaccctg ccctcttctt tttcaatttt ttcaacatca cggcgcatcg gccttggatt taaagttagc caacttgatc ggttttctcg ttcgctccca aagccgtttg gtgggggagg aaagaatatt attggctttt tctcgtcgga ggcaaggata ggctttatgt ctacacacgg tgcgagttca ataccaagca tcgaaccagg accctgggtc tgagcttcca g

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLGIELAPC VDIKPILASD EKKPIIFFPP PPNGFGSERE NRSSWLTLNP RPMRRDVEKI EKEEGRVMNF LAELANPLVR GDEKRRFVIS FFRETDEMSI YEKPERNSGY LAGRFLAKGV YRKPMPDGST VPYTAEDFQV GKEITILERP FRLLDMSEET KRILTVTEQL PSEQRLKELL LLFKQQIQLK FTRGHEAYCT LAPKGVLGYR QVREFLRSCS CSITEDEALL LVHNLVPSSA GVISFNEFMD LVNITSSEHM DEASLTVRSV KSVNMTKDES LKTVAIKTED VKRRKQLAVE LRQKLIQRKG SVQEQFRLIG CHSASSRLNR DVFRHSLNEV MHFNVPKTDE DMLVSLLFDG RADENGDITY KQFQEFLEVQ DDI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttggtatt gaactcgcac cgtgtgtaga cataaagcct atccttgcct ccgacgagaa aaagccaata atattctttc ctcccccacc aaacggcttt gggagcgaac gagaaaaccg atcaagttgg ctaactttaa atccaaggcc gatgcgccgt gatgttgaaa aaattgaaaa agaagagggc agggtcatga attttttggc ggagttggcc aatcctttgg tccgcggtga tgagaagagg agatttgtta tatccttttt tagagaaact gatgaaatgt ctatttatga aaaacccgaa cggaactcag gttaccttgc cgggaggttt cttgcaaaag gtgtttatcg gaaaccaatg ccagatggct caaccgttcc ctacacggca gaggacttcc aggttgggaa ggagattaca atacttgagc gtccttttcg tctcctcgat atgagtgaag agacgaagag gatacttacc gtaacggagc aactcccttc tgagcagcgg ctgaaggagt tacttctttt attcaagcag caaattcagc taaaatttac tcgtggccac gaagcctact gtacattggc tcctaaggga gtattgggct atcgtcaggt tcgcgaattc ttaaggtcgt gtagctgttc tataaccgag gacgaagcac tcctcctcgt acacaacctc gttccttcta gtgccggagt gatttcattt aatgagttca tggatttggt gaacataacc tcttcagaac atatggatga agcttcactg acggtgcgct ctgtaaagag cgtaaacatg acaaaagacg aatcactgaa gacagtagct atcaaaacag aggacgtgaa acgaaggaaa cagcttgcag ttgagttacg ccaaaagttg atccaaagga agggttccgt acaggagcag tttcgcctca ttggttgcca cagcgcatca tcccggttaa atcgagatgt tttccgccac tcactgaacg aagtaatgca ctttaacgtt cctaagacgg acgaggatat gcttgtgtca ctccttttcg atgggagagc tgacgaaaac ggcgacataa cgtacaagca attccaagaa ttccttgagg ttcaggatga tatctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for TrbrA.18248.b.A1.PS01475

PURIFICATION DATe:	4/5/2012
CONCENTRATION:	17.3mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTTGGTATT GAACTCGCAC CGTGTGTAGA CATAAAGCCT ATCCTTGCCT CCGACGAGAA AAAGCCAATA ATATTCTTTC CTCCCCCACC AAACGGCTTT GGGAGCGAAC GAGAAAACCG ATCAAGTTGG CTAACTTTAA ATCCAAGGCC GATGCGCCGT GATGTTGAAA AAATTGAAAA AGAAGAGGGC AGGGTCATGA ATTTTTTGGC GGAGTTGGCC AATCCTTTGG TCCGCGGTGA TGAGAAGAGG AGATTTGTTA TATCCTTTTT TAGAGAAACT GATGAAATGT CTATTTATGA AAAACCCGAA CGGAACTCAG GTTACCTTGC CGGGAGGTTT CTTGCAAAAG GTGTTTATCG GAAACCAATG CCAGATGGCT CAACCGTTCC CTACACGGCA GAGGACTTCC AGGTTGGGAA GGAGATTACA ATACTTGAGC GTCCTTTTCG TCTCCTCGAT ATGAGTGAAG AGACGAAGAG GATACTTACC GTAACGGAGC AACTCCCTTC TGAGCAGCGG CTGAAGGAGT TACTTCTTTT ATTCAAGCAG CAAATTCAGC TAAAATTTAC TCGTGGCCAC GAAGCCTACT GTACATTGGC TCCTAAGGGA GTATTGGGCT ATCGTCAGGT TCGCGAATTC TTAAGGTCGT GTAGCTGTTC TATAACCGAG GACGAAGCAC TCCTCCTCGT ACACAACCTC GTTCCTTCTA GTGCCGGAGT GATTTCATTT AATGAGTTCA TGGATTTGGT GAACATAACC TCTTCAGAAC ATATGGATGA AGCTTCACTG ACGGTGCGCT CTGTAAAGAG CGTAAACATG ACAAAAGACG AATCACTGAA GACAGTAGCT ATCAAAACAG AGGACGTGAA ACGAAGGAAA CAGCTTGCAG TTGAGTTACG CCAAAAGTTG ATCCAAAGGA AGGGTTCCGT ACAGGAGCAG TTTCGCCTCA TTGGTTGCCA CAGCGCATCA TCCCGGTTAA ATCGAGATGT TTTCCGCCAC TCACTGAACG AAGTAATGCA CTTTAACGTT CCTAAGACGG ACGAGGATAT GCTTGTGTCA CTCCTTTTCG ATGGGAGAGC TGACGAAAAC GGCGACATAA CGTACAAGCA ATTCCAAGAA TTCCTTGAGG TTCAGGATGA TATCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA