SSGCID: Target Page

TrbrA.18247.b

Protein kinase, putative (EC 2.7.1.-)

CENTER ID:	TrbrA.18247.b
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.18247.b.HZ2.GE43757	kinase domain		22	344

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.7.6220
UniProt:	Q57VI9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFHSLQSTL TEARALASSS KTVSFDHHYE LLEELGKGAY GTVWKCCRRH DSLKRPYGVK IINKREAGAK GLRSVMGEVE TMSLLSHPNI VRLEETFHDD QTLWIVMEYM AGGELQRALK RDGSFSEVQT RRITMQLLFA LEFIHQKGIV HRDLKPENCL LSEGDLVCKI SDFGFAVLVG VDQCLMSFCG TTVFMAPEIF SDTNYGKPVD MWALGVMVYL MFTGEYPFLG RTQKETTEAI LKANYNMKDG KISEGSSALH DFISKLLVVD PNRRYSAREA LKHPWIKLGM NMEIPDDEPK APKRSGLRPR GVFRALSIAI MAAHRLLYLR YCRMLDSSDC GNCAMLRNFT YVVSGRYEPP CPSLECSGVF LRHPRGLPLL LPMVEVSRTL ESLDLSNNNI FNLTFLQQLV KVVGNHPSLV SLNLSNNPIP ALAGRGLLRL ARSPQSRLTY LGLEGTQLSA EALAQISSAL KERLAAAPTL CLSMQSCTGP RPLEQVSPTQ SRSARTTRSR SQHGSAHTSL MNPSRSPQSK GPRLPPINSK RRNER

NT Sequence

atggacttcc attcgttgca gagtacgttg acggaggccc gcgcattagc ctcttcgtcc aagacagttt cttttgacca tcattacgaa cttttggagg aacttggaaa aggggcgtac ggaacggtat ggaaatgttg ccggcggcac gactcgttga agcgaccgta cggagtaaaa attatcaaca agcgagaagc gggagcaaaa gggttgcgta gtgtgatggg tgaggttgag accatgagtc ttctcagtca tccaaatatt gtgagattag aggagacgtt tcacgatgat caaacccttt ggattgtaat ggaatatatg gctggtggtg agttacagcg tgcgctgaaa cgtgatggat catttagtga agtccagact cgtcgtatta ctatgcagct tctgtttgcg ttagagttca tccaccaaaa gggtattgtt catcgagacc ttaaaccgga gaattgtctt ctttcggagg gtgacttagt gtgtaagata tctgactttg gttttgccgt gctcgttggc gtggatcagt gcctcatgtc cttttgtggc acaacggtgt ttatggcacc tgagatattc agcgacacaa actatggcaa accggtggac atgtgggcat taggcgtgat ggtgtatctt atgttcacag gtgaatatcc atttctgggt cgtacgcaga aagagacgac ggaggctatt ttgaaggcca attacaacat gaaggatgga aagattagtg aggggtcatc ggctttgcat gactttatat ccaaattact tgtggttgat ccgaaccgcc ggtatagtgc acgggaggct ctaaagcacc cgtggatcaa actaggtatg aacatggaaa ttcccgatga tgagccgaag gcgccaaagc gaagtgggct acgtccccgc ggcgtgtttc gtgctctcag catcgctatt atggctgccc accggctgct ttacctgcgg tactgccgca tgctcgatag cagtgattgc ggaaactgtg caatgctccg caacttcacc tacgtggtgt ctggtaggta cgaaccaccg tgtccgtcgt tagagtgtag tggtgtattc ctgcgtcatc ctcgcggttt gccattgtta ctcccaatgg tggaggtgag ccgaactctt gagtcgttag atctgagcaa caacaacatc ttcaatctca cgttcctgca gcaacttgtg aaggtggttg gcaatcatcc gtctttggtg tcactgaact tgtccaacaa ccccattccg gcactggcag gtcgtggtct gctccgtctt gcccgaagcc cgcagtcacg acttacatat ctcgggttgg agggaacaca actctctgct gaagcccttg ctcaaatctc atcagcactg aaggagcgac tggccgccgc cccaacgttg tgtctttcca tgcagagttg cacaggccca cgccctttag agcaggtgag tcccacgcag tcccgatccg cccgcacgac ccgttcgcga tcacaacacg ggtcggcaca cacgtcttta atgaatcctt cgcgttcccc gcagtcgaag gggccgcggc ttccacctat caactccaag cgccgcaacg agagatga

Details for TrbrA.18247.b.HZ2.GE43757

HARVESTED ON:	7/6/2020
SEQUENCED ON:	6/23/2020
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	49

Validated AA Sequence

MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMT VSFDHHYELL EELGKGAYGT VWKCCRRHDS LKRPYGVKII NKREAGAKGL RSVMGEVETM SLLSHPNIVR LEETFHDDQT LWIVMEYMAG GELQRALKX

Validated NT Sequence

tgnncagttt ccgcaatcac tgctatcgag catgcggcan nnccncaggt aaagcagccg gtgggcagcc ataatagcga tgctgagagc gcgaaacacg ccacggggac gtagcccact tcgctttggc gccttcggct catcatcggg aatttccatg ttcataccta gtttgatcca cgggtgcttt agagcctcgc gtgcactata ccggcggttc ggatcaacca caagtaattt ggatataaag tcatgcaaag ccgatgaccc ctcactaatc tttccatcct tcatgntgta attggccttc agaatagcct ccgtcnnctc tttctgcgta cgacccagaa atggatattc acctgngaac ataagataca ccattacgcc taatgcccac atgtccaccg gtttgccata gtttgtgtcg ctgaatatct caggtgccat aaacaccgtt gtgccacaaa aggacatgag gnactgatcc acgccaacga gcacggcaaa accaaagtca gatatcttac acactaagtc accctccgaa agaagacaat tctccggttt aaggtctcga tgaacaatac ccttttggtg gatgaactct aacgcaaaca gaagctgcat agtaatacga cgagtctgga cttcactaaa tgatccatca cnntttcagc gcacgctgca actcaccacc agccatatat tccattacaa tccaaagggt ttgatcatcg tgaaacgtct cctctaatcg cacaatattt ggatgactga gaagactcat ggtctcaacc tcacccatca cactacgcaa cccttttgct cccgcttctc gcttgttgat aatttttact ccgtacggtc gcttcaacga gtcgtgccgc cggcaacatt tccataccgt tccgtacgcc ccttttccaa gttcctccaa aagttcgtaa tgatggtcaa aagaaactgt catcgaacca ggaccctggg tctgagcttc cagggtacca ccaatctgtt ctctgtgagc ctcaataata tcgttatcct ccatgtccaa atcttcaggg gtctgatcag cttgaattct aataccgtcg tacaagaatc ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc cttcttaaag gagtggtctt tttgatcttg aagaagatct ctgaagatcc atcggacacc tttaaattga tgtgagtctc aggcttgact tctggcttga cctctggctt agcttcttga ttgacttctg agtccgactt catatgatgg tgatggtgat gagccat

Expected Protein Sequence

MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSTV SFDHHYELLE ELGKGAYGTV WKCCRRHDSL KRPYGVKIIN KREAGAKGLR SVMGEVETMS LLSHPNIVRL EETFHDDQTL WIVMEYMAGG ELQRALKRDG SFSEVQTRRI TMQLLFALEF IHQKGIVHRD LKPENCLLSE GDLVCKISDF GFAVLVGVDQ CLMSFCGTTV FMAPEIFSDT NYGKPVDMWA LGVMVYLMFT GEYPFLGRTQ KETTEAILKA NYNMKDGKIS EGSSALHDFI SKLLVVDPNR RYSAREALKH PWIKLGMNME IPDDEPKAPK RSGLRPRGVF RALSIAIMAA HRLLYLRYCR MLDSSDCGNC A

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga cagtttcttt tgaccatcat tacgaacttt tggaggaact tggaaaaggg gcgtacggaa cggtatggaa atgttgccgg cggcacgact cgttgaagcg accgtacgga gtaaaaatta tcaacaagcg agaagcggga gcaaaagggt tgcgtagtgt gatgggtgag gttgagacca tgagtcttct cagtcatcca aatattgtga gattagagga gacgtttcac gatgatcaaa ccctttggat tgtaatggaa tatatggctg gtggtgagtt acagcgtgcg ctgaaacgtg atggatcatt tagtgaagtc cagactcgtc gtattactat gcagcttctg tttgcgttag agttcatcca ccaaaagggt attgttcatc gagaccttaa accggagaat tgtcttcttt cggagggtga cttagtgtgt aagatatctg actttggttt tgccgtgctc gttggcgtgg atcagtgcct catgtccttt tgtggcacaa cggtgtttat ggcacctgag atattcagcg acacaaacta tggcaaaccg gtggacatgt gggcattagg cgtgatggtg tatcttatgt tcacaggtga atatccattt ctgggtcgta cgcagaaaga gacgacggag gctattttga aggccaatta caacatgaag gatggaaaga ttagtgaggg gtcatcggct ttgcatgact ttatatccaa attacttgtg gttgatccga accgccggta tagtgcacgg gaggctctaa agcacccgtg gatcaaacta ggtatgaaca tggaaattcc cgatgatgag ccgaaggcgc caaagcgaag tgggctacgt ccccgcggcg tgtttcgtgc tctcagcatc gctattatgg ctgcccaccg gctgctttac ctgcggtact gccgcatgct cgatagcagt gattgcggaa actgtgcata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a