SSGCID: Target Page

TrbrA.18217.a

DNA topoisomerase type IB small subunit

CENTER ID:	TrbrA.18217.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.18217.a.B1.GE34470	full length		1	273

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb09.160.5070
RefSeq:	XP_827055.1
UniProt:	Q38EP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSANSEVRN GGSSSVAAAQ PRAASPAKIP VKVPYSLAKV PMPRLVPAKM PAPKRAAESE AQHRGNREDS DDALLESSAP SKKPKKEESK KAPLVETESS SSSEYSSSDS DDTDASDSES EGSKTSTVSD SSDISDDEES VSEDNLTFFE LAERSGLMND IKNKVNSGEG EQEEEEEVHI DGKPPRPKFL RSFPRDIEAE IGKHEKRLKD VENMLKMKQD NKTVSLGTSK VNYIDPRIVC SWANENNVPI SRLFSATLQK KFPWALKARD FTF

NT Sequence

atgtcttcgg ctaactcgga ggtacgtaac ggtggttcat cttcagttgc cgccgcgcaa ccccgtgcgg catcgcccgc gaagatcccc gtgaaggtac cctactcact tgccaaagtt ccaatgccgc ggcttgtacc ggcgaagatg ccggcaccaa agcgagcagc tgagagcgag gctcaacatc gcggcaacag ggaagacagt gacgacgctc tgcttgagtc ctccgcaccc tctaaaaaac caaagaagga ggagtcaaaa aaggctcctc tagtggaaac ggagtctagc agtagcagcg agtacagttc ttctgacagc gatgataccg atgctagtga cagcgaaagc gagggctcga aaacgtctac agtttccgat tcaagtgaca tttcggacga cgaagaaagc gtttctgagg acaatctgac attttttgaa ttagccgaga gaagcggcct catgaatgat atcaagaata aggtaaactc tggggaaggg gaacaggagg aggaggagga ggtgcacatc gacgggaagc caccacggcc caagtttctt cgtagctttc ctcgcgatat tgaggcggaa atcgggaaac acgagaaacg cctcaaggat gttgagaata tgttaaaaat gaaacaggat aataaaactg tttcgttggg cacaagtaag gtaaattata tagacccacg gattgtgtgc tcctgggcca acgagaacaa cgtccccatt tcgaggctgt tttctgcgac acttcaaaaa aagttccctt gggcactgaa ggcaagggac tttacgtttt ga

Details for TrbrA.18217.a.B1.GE34470

HARVESTED ON:	2/6/2012
SEQUENCED ON:	2/21/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS SANSEVRNGG SSSVAAAQPR AASPAKIPVK VPYSLAKVPM PRLVPAKMPA PKRAAESEAQ HRGNREDSDD ALLESSAPSK KPKKEESKKA PLVETESSSS SEYSSSDSDD TDASDSESEG SKTSTVSDSS DISDDEESVS EDNLTFFELA ERSGLMNDIK NKVNSGEGEQ EEEEEVHIDG KPPRPKFLRS FPRDIEAEIG KHEKRLKDVE NMLKMKQDNK TVSLGTSKVN YIDPRIVCSW ANENNVPISR LFSATLQKKF PWALKARDFT F

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt cggctaactc ggaggtacgt aacggtggtt catcttcagt tgccgccgcg caaccccgtg cggcatcgcc cgcgaagatc cccgtgaagg taccctactc acttgccaaa gttccaatgc cgcggcttgt accggcgaag atgccggcac caaagcgagc agctgagagc gaggctcaac atcgcggcaa cagggaagac agtgacgacg ctctgcttga gtcctccgca ccctctaaaa aaccaaagaa ggaggagtca aaaaaggctc ctctagtgga aacggagtct agcagtagca gcgagtacag ttcttctgac agcgatgata ccgatgctag tgacagcgaa agcgagggct cgaaaacgtc tacagtttcc gattcaagtg acatttcgga cgacgaagaa agcgtttctg aggacaatct gacatttttt gaattagccg agagaagcgg cctcatgaat gatatcaaga ataaggtaaa ctctggggaa ggggaacagg aggaggagga ggaggtgcac atcgacggga agccaccacg gcccaagttt cttcgtagct ttcctcgcga tattgaggcg gaaatcggga aacacgagaa acgcctcaag gatgttgaga atatgttaaa aatgaaacag gataataaaa ctgtttcgtt gggcacaagt aaggtaaatt atatagaccc acggattgtg tgctcctggg ccaacgagaa caacgtcccc atttcgaggc tgttttctgc gacacttcaa aaaaagttcc cttgggcact gaaggcaagg gactttacgt tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat