TrbrA.17369.a

vacuolar ATP synthase subunit B, putative

CENTER ID: TrbrA.17369.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.17369.a.B1.GE34468 full length 1 495
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb11.01.3560
RefSeq: XP_829232.1
UniProt: Q383B1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGHNEEVRM LSKQELLTEH IKELQKDYSI KPRLEYTTIR AVNGPLVILG NVRLPTFAEI VNIELADGTL RRGQVLEVDG DKAVVQVFEG TSGIDVMRSK CEFTGKVMEL GVSEDMLGRV FNGSGIPIDD GPPVLAEQYR DIQGIPINPR ARVYPEEMIQ TGISSIDVMT SISRGQKIPL FSGAGLPHNE IAAQIVRQAG LVRREGKQED FCIVFAAMGV NQETARFFRT EFEENGSMEK TVLFMNLAND PTIERIVTPR LALTTAEYLA YDCGKHVLVI LTDMSSYADA LREVSAAREE VPGRRGFPGY MYTDLACIYE RAGRVLGRAG SITQIPILTM PNDDITHPIP DLTGYITEGQ IYVDRQLHNR QLYPPINILP SLSRLMKNAI GEGMTRKDHS GVSNQMYAAY AVSRDILAMK AVVGEETLSS EDLLHLEFLE KFEKKFICQG FYESRDVFQS LDLCWQLLRI FPKVLLNKID VKTRNEFYDR QPANR
NT Sequence
atgggcggac ataatgaaga agtgcggatg ctctctaagc aggagcttct cacggaacac attaaagaac tgcagaagga ctactctatc aaaccacgtc tcgagtacac aaccatccgt gcagttaatg gaccgcttgt gattttaggg aacgtgcggc tgccgacatt tgcggagatt gtaaacattg agttggctga cggcacattg cggcgcgggc aggtgctgga agtggacggt gataaggctg ttgtgcaggt gtttgaggga acctccggta ttgacgtaat gcgctcaaag tgtgagttca cagggaaggt gatggaattg ggcgtgagtg aagatatgct gggacgtgtc ttcaatggct ccggcattcc aatcgacgat gggccgccgg tgttagccga gcagtatcgc gatattcaag gtattcccat taacccacgc gcccgtgtgt acccggagga aatgattcaa accggtattt cctccattga tgttatgact tccatttcgc gtgggcagaa aattcccctc ttctccggcg ctggtcttcc acacaatgaa attgcggcgc agatcgtccg ccaggccggt ttggttcgca gggaagggaa acaggaggat ttttgtattg tgttcgcggc catgggtgta aaccaggaaa ccgctcgctt cttccgcacc gagttcgagg agaacggctc tatggaaaaa accgttctgt tcatgaactt ggcgaacgat cccaccattg aacgcatcgt cacgcctcgc cttgctctta ccacagcgga atatctcgcg tatgactgcg ggaaacacgt gctagtgatc ctcactgaca tgagctcgta tgcggatgcc ttacgtgagg tgtctgcggc gcgtgaggag gtccctggcc gtcgtggttt ccccggttac atgtacacag atctagcatg catttatgaa cgggccgggc gtgtgctggg tcgtgctgga tccattacac aaataccaat tctcaccatg ccgaatgatg atattacaca tcccattcct gacctcacgg ggtacatcac agaagggcaa atatacgtgg accgccagtt gcataaccgt cagttgtatc ctcccattaa cattttgcca tcactcagcc ggctgatgaa gaatgccatt ggtgagggaa tgacacgaaa ggatcacagc ggagtcagca atcaaatgta cgccgcgtat gccgtgagtc gcgacatttt ggccatgaag gctgttgttg gtgaggagac gctcagcagc gaagatctgc tgcatctgga gtttctggag aagttcgaga agaagttcat ttgccaaggt ttttatgagt cacgcgatgt gtttcagagc ttggacctct gctggcagtt gctccgcatt ttcccgaagg tgttgctcaa caaaattgat gtgaagacga gaaatgaatt ttacgatcgt caacccgcca accgttga
Details for TrbrA.17369.a.B1.GE34468
HARVESTED ON: 2/6/2012
SEQUENCED ON: 2/21/2012
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG GHNEEVRMLS KQELLTEHIK ELQKDYSIKP RLEYTTIRAV NGPLVILGNV RLPTFAEIVN IELADGTLRR GQVLEVDGDK AVVQVFEGTS GIDVMRSKCE FTGKVMELGV SEDMLGRVFN GSGIPIDDGP PVLAEQYRDI QGIPINPRAR VYPEEMIQTG ISSIDVMTSI SRGQKIPLFS GAGLPHNEIA AQIVRQAGLV RREGKQEDFC IVFAAMGVNQ ETARFFRTEF EENGSMEKTV LFMNLANDPT IERIVTPRLA LTTAEYLAYD CGKHVLVILT DMSSYADALR EVSAAREEVP GRRGFPGYMY TDLACIYERA GRVLGRAGSI TQIPILTMPN DDITHPIPDL TGYITEGQIY VDRQLHNRQL YPPINILPSL SRLMKNAIGE GMTRKDHSGV SNQMYAAYAV SRDILAMKAV VGEETLSSED LLHLEFLEKF EKKFICQGFY ESRDVFQSLD LCWQLLRIFP KVLLNKIDVK TRNEFYDRQP ANR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg gacataatga agaagtgcgg atgctctcta agcaggagct tctcacggaa cacattaaag aactgcagaa ggactactct atcaaaccac gtctcgagta cacaaccatc cgtgcagtta atggaccgct tgtgatttta gggaacgtgc ggctgccgac atttgcggag attgtaaaca ttgagttggc tgacggcaca ttgcggcgcg ggcaggtgct ggaagtggac ggtgataagg ctgttgtgca ggtgtttgag ggaacctccg gtattgacgt aatgcgctca aagtgtgagt tcacagggaa ggtgatggaa ttgggcgtga gtgaagatat gctgggacgt gtcttcaatg gctccggcat tccaatcgac gatgggccgc cggtgttagc cgagcagtat cgcgatattc aaggtattcc cattaaccca cgcgcccgtg tgtacccgga ggaaatgatt caaaccggta tttcctccat tgatgttatg acttccattt cgcgtgggca gaaaattccc ctcttctccg gcgctggtct tccacacaat gaaattgcgg cgcagatcgt ccgccaggcc ggtttggttc gcagggaagg gaaacaggag gatttttgta ttgtgttcgc ggccatgggt gtaaaccagg aaaccgctcg cttcttccgc accgagttcg aggagaacgg ctctatggaa aaaaccgttc tgttcatgaa cttggcgaac gatcccacca ttgaacgcat cgtcacgcct cgccttgctc ttaccacagc ggaatatctc gcgtatgact gcgggaaaca cgtgctagtg atcctcactg acatgagctc gtatgcggat gccttacgtg aggtgtctgc ggcgcgtgag gaggtccctg gccgtcgtgg tttccccggt tacatgtaca cagatctagc atgcatttat gaacgggccg ggcgtgtgct gggtcgtgct ggatccatta cacaaatacc aattctcacc atgccgaatg atgatattac acatcccatt cctgacctca cggggtacat cacagaaggg caaatatacg tggaccgcca gttgcataac cgtcagttgt atcctcccat taacattttg ccatcactca gccggctgat gaagaatgcc attggtgagg gaatgacacg aaaggatcac agcggagtca gcaatcaaat gtacgccgcg tatgccgtga gtcgcgacat tttggccatg aaggctgttg ttggtgagga gacgctcagc agcgaagatc tgctgcatct ggagtttctg gagaagttcg agaagaagtt catttgccaa ggtttttatg agtcacgcga tgtgtttcag agcttggacc tctgctggca gttgctccgc attttcccga aggtgttgct caacaaaatt gatgtgaaga cgagaaatga attttacgat cgtcaacccg ccaaccgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat