TrbrA.10384.a

cell division control protein 2 homolog 2,cdc2-related kinase

CENTER ID: TrbrA.10384.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.10384.a.A1.GE34285 full length 1 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.7.7360
RefSeq: XP_846356.1
UniProt: Q57TX3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQVQVQEGQT ACDGSLRPLP SAGPASFVPR SLRPAPLRGT STPDRYSRIE KVGEGSYGIV YKCHDNFTGR TVAMKRIPLI VNDGGVPSTA VREVSLLREL NHPYVVRLLD VVLHEAKLLL IFEYMEQDLQ GMLKQRNTAF VGGKLRRIMF QLLLGLHECH SRRFVHRDIK PSNILIDRKE SVVKLADFGL GRAFRVPLQT YTTEVMTLWY RAPEVLLGDK QYLPAVDVWS MGCVFAELAR RRSLFAGDTA INQLFSIFQL LGTPTEATWR GVTSLPHHNV NFPRWTAKPL RTAVPALDDD GVDLLRRMLC YNPRERITAY EALQHSYFDE VREEEVEKLM RFNGA
NT Sequence
atgcaggtgc aggtgcagga aggacagacg gcatgtgatg gttcgctgcg accacttcct tctgctggcc ctgcttcttt cgtgccgcgc tcgctgcgcc ccgccccgct ccgaggtacg tccaccccgg accggtatag ccgaatagag aaggttggtg aaggttccta cggtattgtg tacaagtgcc acgacaactt taccggccgc actgtagcta tgaagcggat cccgcttatc gtaaacgacg gcggcgtacc ttccaccgcc gtccgcgagg tatcactgct acgcgaactg aatcacccat acgtggtacg gcttctcgat gtggtactgc atgaggcgaa gctgttgctc atatttgaat acatggagca agacttgcaa gggatgctga agcaacggaa caccgccttc gttggcggca agcttcgtcg tatcatgttt cagctgcttc ttggcttgca tgagtgccac agtcgccgct tcgtccaccg tgacattaag ccaagcaata ttcttattga ccgcaaggag tctgtcgtga aacttgccga ttttggactt ggtcgtgcct tccgtgtgcc cctgcagaca tacacaacgg aggttatgac gctgtggtac cgtgcccccg aggtgctcct tggggacaaa cagtacctcc ccgctgtgga tgtgtggtcg atggggtgtg tctttgctga acttgcccgt cgcaggtcgc tcttcgcggg ggataccgcc atcaatcagc tcttttccat attccagttg ctcggcacac cgacggaggc aacttggcgc ggcgtcacat cgctacccca ccacaacgtt aacttcccgc ggtggaccgc gaagccactg cgcactgcag ttcccgcgtt ggacgacgac ggtgtagatt tactgcggcg catgctgtgc tacaacccga gggaacgtat aaccgcttat gaggctcttc aacactcata ctttgatgag gtgcgtgagg aagaagtgga aaagttgatg cggttcaacg gagcttaa
Details for TrbrA.10384.a.A1.GE34285
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQVQVQEGQ TACDGSLRPL PSAGPASFVP RSLRPAPLRG TSTPDRYSRI EKVGEGSYGI VYKCHDNFTG RTVAMKRIPL IVNDGGVPST AVREVSLLRE LNHPYVVRLL DVVLHEAKLL LIFEYMEQDL QGMLKQRNTA FVGGKLRRIM FQLLLGLHEC HSRRFVHRDI KPSNILIDRK ESVVKLADFG LGRAFRVPLQ TYTTEVMTLW YRAPEVLLGD KQYLPAVDVW SMXXVFAELA RRSRSSRGIP PSISSFPYSS CSAHRRRQLG AXSHRYPXXN VNFPXXTXXX LXTAXPXXXX DXVDLLXRML XXXRGTXXXX XXXXXT
Validated NT Sequence
ttgttnnntt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgcaggtgc aggtgcagga aggacagacg gcatgtgatg gttcgctgcg accacttcct tctgctggcc ctgcttcttt cgtgccgcgc tcgctgcgcc ccgccccgct ccgaggtacg tccaccccgg accggtatag ccgaatagag aaggttggtg aaggttccta cggtattgtg tacaagtgcc acgacaactt taccggccgc actgtagcta tgaagcggat cccgcttatc gtaaacgacg gcggcgtacc ttccaccgcc gtccgcgagg tatcactgct acgcgaactg aatcacccat acgtggtacg gcttctcgat gtggtactgc atgaggcgaa gctgttgctc atatttgaat acatggagca agacttgcaa gggatgctga agcaacggaa caccgccttc gttggcggca agcttcgtcg tatcatgttt cagctgcttc ttggcttgca tgagtgccac agtcgccgct tcgtccaccg tgacattaag ccaagcaata ttcttattga ccgcaaggag tctgtcgtga aacttgccga ttttggactt ggtcgtgcct tccgtgtgcc cctgcagaca tacacaacgg aggttatgac gctgtggtac cgtgcccccg aggtgctcct tggggacaaa cagtacctcc ccgctgtgga tgtgtggtcg atgnnnngtg tctttgctga acttgcccgt cgcagtcgct cttcgcgggg gataccgcca tcaatcagct cttttccata ttccagttgc tcggcacacc gacggaggca acttggngcn gngtcacatc gctacccccn cnacaacgtt aacttcccgc ngnggaccnc gaancnnctg cncactgcan tnccnncgtn ngannacgac gnngtagatt tactgcnncg catgctgtnc tannnncgng gnacgtannn nnntnnnnnn tntnnnnctc ntacttgatn nnnngnnnnn nnnnnnannn nntgnnnnca nnnnnctnnn aannnnnnnn annntgcann nnnnnnnnnn nnnnnnngnn nnnnncnnaa nnnnnnnnnn nnnnnnnnna nnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQVQVQEGQ TACDGSLRPL PSAGPASFVP RSLRPAPLRG TSTPDRYSRI EKVGEGSYGI VYKCHDNFTG RTVAMKRIPL IVNDGGVPST AVREVSLLRE LNHPYVVRLL DVVLHEAKLL LIFEYMEQDL QGMLKQRNTA FVGGKLRRIM FQLLLGLHEC HSRRFVHRDI KPSNILIDRK ESVVKLADFG LGRAFRVPLQ TYTTEVMTLW YRAPEVLLGD KQYLPAVDVW SMGCVFAELA RRRSLFAGDT AINQLFSIFQ LLGTPTEATW RGVTSLPHHN VNFPRWTAKP LRTAVPALDD DGVDLLRRML CYNPRERITA YEALQHSYFD EVREEEVEKL MRFNGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggtgcag gtgcaggaag gacagacggc atgtgatggt tcgctgcgac cacttccttc tgctggccct gcttctttcg tgccgcgctc gctgcgcccc gccccgctcc gaggtacgtc caccccggac cggtatagcc gaatagagaa ggttggtgaa ggttcctacg gtattgtgta caagtgccac gacaacttta ccggccgcac tgtagctatg aagcggatcc cgcttatcgt aaacgacggc ggcgtacctt ccaccgccgt ccgcgaggta tcactgctac gcgaactgaa tcacccatac gtggtacggc ttctcgatgt ggtactgcat gaggcgaagc tgttgctcat atttgaatac atggagcaag acttgcaagg gatgctgaag caacggaaca ccgccttcgt tggcggcaag cttcgtcgta tcatgtttca gctgcttctt ggcttgcatg agtgccacag tcgccgcttc gtccaccgtg acattaagcc aagcaatatt cttattgacc gcaaggagtc tgtcgtgaaa cttgccgatt ttggacttgg tcgtgccttc cgtgtgcccc tgcagacata cacaacggag gttatgacgc tgtggtaccg tgcccccgag gtgctccttg gggacaaaca gtacctcccc gctgtggatg tgtggtcgat ggggtgtgtc tttgctgaac ttgcccgtcg caggtcgctc ttcgcggggg ataccgccat caatcagctc ttttccatat tccagttgct cggcacaccg acggaggcaa cttggcgcgg cgtcacatcg ctaccccacc acaacgttaa cttcccgcgg tggaccgcga agccactgcg cactgcagtt cccgcgttgg acgacgacgg tgtagattta ctgcggcgca tgctgtgcta caacccgagg gaacgtataa ccgcttatga ggctcttcaa cactcatact ttgatgaggt gcgtgaggaa gaagtggaaa agttgatgcg gttcaacgga gcttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa