SSGCID: Target Page

TrbrA.10317.a

cell division related protein kinase 2, putative,CDC2-related protein kinase

CENTER ID:	TrbrA.10317.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.10317.a.A1.GE34284	full length		1	311

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.10.4990

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTMLGALTGR QLSSGLKDQF DRYNRMDILG EGTYGVVYRA VDRATGQIVA LKKVRLDRTD EGIPQTALRE VSILQEIHHP NIVNLLDVIC ADGKLYLIFE YVDHDLKKAL EKRGGAFTGT TLKKIIYQLL EGLSFCHRHR IVHRDLKPAN ILVTTDNSVK IADFGLARAF QIPMHTYTHE VVTLWYRAPE ILLGEKHYTP AVDMWSIGCI FAELARGKVL FRGDSEIGQL FEIFQVLGTP MDAEGSWLGV SSLPDYRDVF PKWSGKPLTQ VLPALDGDAV DLLSQMLRYN PAERISAKAA LQHPWFSDAM F

NT Sequence

atgacaatgc ttggggcgtt aaccggtcga caactttcct ctggtcttaa ggatcagttc gaccgctata atcgaatgga catacttgga gaaggaacgt atggagttgt gtaccgtgct gttgacaggg caacgggaca gatcgtcgca ctgaagaaag tgagattaga tcgcaccgat gagggaatac ctcaaacagc tcttcgggag gtatctattt tgcaagaaat ccatcacccc aacattgtta atcttctaga tgttatatgt gccgatggga agctttactt gatatttgag tatgttgatc atgatctcaa aaaggctctc gagaagaggg gtggagcatt cacgggaact acactaaaaa agataattta ccagctactc gagggacttt cgttttgtca tcggcaccgc attgtccatc gagacctaaa accagcaaac atacttgtca ccacggataa ttccgttaaa atagcagact ttggattggc tcgcgctttc cagattccga tgcacactta cactcacgag gttgtcacac tctggtatag agcgccagag attctcctcg gtgaaaagca ctacaccccg gcagtggata tgtggagcat tggttgtatt tttgctgaac tagctcgagg taaggtactc tttcgtggtg acagtgagat tggacagtta tttgagattt ttcaggtgct tggcacccca atggatgctg aggggtcgtg gttgggggtg tcgtctcttc cagactatcg tgacgtcttt ccaaagtgga gtggaaaacc cctcactcag gtgttaccag cgcttgacgg tgatgccgtt gatctgcttt ctcagatgct gaggtataac cctgctgaac gtatttcagc caaggcggcg ctgcaacatc catggtttag tgatgccatg ttttag

Details for TrbrA.10317.a.A1.GE34284

HARVESTED ON:	12/19/2011
SEQUENCED ON:	12/28/2011
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MTMLGALTGR QLSSGLKDQF DRYNRMDILG EGTYGVVYRA VDRATGQIVA LKKVRLDRTD EGIPQTALRE VSILQEIHHP NIVNLLDVIC ADGKLYLIFE YVDHDLKKAL EKRGGAFTGT TLKKIIYQLL EGLSFCHRHR IVHRDLKPAN ILVTTDNSVK IADFGLARAF QIPMHTYTHE VVTLWYRAPE ILLGEKHYTP AVDMWSIGCI FAELARGKVL FRGDSEIGQL FEIFQVLGTP MDAEGSWLGV SSLPDYRDVF PKWNGKPLTQ VLPALDGDAV DLLSQMLRYN PAERISAKAA LQHPWFSDAM F

Validated NT Sequence

aaaacatggc atcactaaac catggatgtt gcagtgccgc cttggctgaa atacgttcag cagggttata cctcagcatc tgagaaagca gatcaacggc atcaccgtca agcgctggta acacctgagt gaggggtttt ccattccact ttggaaagac gtcacgatag tctggaagag acgacacccc caaccacgac ccctcagcat ccattggggt gccaagcacc tgaaaaatct caaataactg tccaatctca ctgtcaccac gaaagagtac cttacctcga gctagttcag caaaaataca accaatgctc cacatatcca ctgccggggt gtagtgcttt tcaccgagga gaatctctgg cgctctatac cagagtgtga caacctcgtg agtgtaagtg tgcatcggaa tctggaaagc gcgagccaat ccaaagtctg ctattttaac ggaattatcc gtggtgacaa gtatgtttgc tggttttagg tctcgatgga caatgcggtg ccgatgacaa aacgaaagtc cctcgagtag ctggtaaatt atctttttta gtgtagttcc cgtgaatgct ccacccctct tttcgagagc ctttttgaga tcatgatcaa catactcaaa tatcaagtaa agcttcccat cggcacatat aacatctaga agattaacaa tgttggggtg atggatttct tgcaaaatag atacttcccg aagagctgtt tgaggtattc cctcatcggt gcgatctaat ctcactttct tcagtgcgac gatctgtccc gttgccctgt caacagcacg gtatacaact ccatacgttc cttctccaag tatgtccatt cgattatagc ggtcgaactg atccttaaga ccagaggaaa gttgtcgacc ggttaacgcc ccaagcattg tcatcgaacc aggaccctgg gtctgagctt ccag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTMLGALTG RQLSSGLKDQ FDRYNRMDIL GEGTYGVVYR AVDRATGQIV ALKKVRLDRT DEGIPQTALR EVSILQEIHH PNIVNLLDVI CADGKLYLIF EYVDHDLKKA LEKRGGAFTG TTLKKIIYQL LEGLSFCHRH RIVHRDLKPA NILVTTDNSV KIADFGLARA FQIPMHTYTH EVVTLWYRAP EILLGEKHYT PAVDMWSIGC IFAELARGKV LFRGDSEIGQ LFEIFQVLGT PMDAEGSWLG VSSLPDYRDV FPKWSGKPLT QVLPALDGDA VDLLSQMLRY NPAERISAKA ALQHPWFSDA MF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaatgctt ggggcgttaa ccggtcgaca actttcctct ggtcttaagg atcagttcga ccgctataat cgaatggaca tacttggaga aggaacgtat ggagttgtgt accgtgctgt tgacagggca acgggacaga tcgtcgcact gaagaaagtg agattagatc gcaccgatga gggaatacct caaacagctc ttcgggaggt atctattttg caagaaatcc atcaccccaa cattgttaat cttctagatg ttatatgtgc cgatgggaag ctttacttga tatttgagta tgttgatcat gatctcaaaa aggctctcga gaagaggggt ggagcattca cgggaactac actaaaaaag ataatttacc agctactcga gggactttcg ttttgtcatc ggcaccgcat tgtccatcga gacctaaaac cagcaaacat acttgtcacc acggataatt ccgttaaaat agcagacttt ggattggctc gcgctttcca gattccgatg cacacttaca ctcacgaggt tgtcacactc tggtatagag cgccagagat tctcctcggt gaaaagcact acaccccggc agtggatatg tggagcattg gttgtatttt tgctgaacta gctcgaggta aggtactctt tcgtggtgac agtgagattg gacagttatt tgagattttt caggtgcttg gcaccccaat ggatgctgag gggtcgtggt tgggggtgtc gtctcttcca gactatcgtg acgtctttcc aaagtggagt ggaaaacccc tcactcaggt gttaccagcg cttgacggtg atgccgttga tctgctttct cagatgctga ggtataaccc tgctgaacgt atttcagcca aggcggcgct gcaacatcca tggtttagtg atgccatgtt ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa