TrbrA.01606.a

iron superoxide dismutase, putative

CENTER ID: TrbrA.01606.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.01606.a.A1.GE34282 full length 1 238
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.5.3350
RefSeq: XP_845007.1
UniProt: Q57Z58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSVMMRCAT GHHMAPLGLA CMQYATLPNL KKPNGAAAEL PPLQFNWKDG CAPVLSPRQL ELHYTKHHKA YVDKLNALAG ATYDGKTMED IIVALANDSE KKVLFNQAAQ HFNHSFYWLC ITPNGKPMPK SLESALTAQF GSVDGFKDTF MQAGANNFGS GWTWLCVDPK NKGKPLVIDN TSNAGCPITK GLRPVFTVDV WEHAYYKDFE NRRVDYLKEI WTIVDWEFVS RTYEQAMK
NT Sequence
atgaggtctg tcatgatgcg ttgcgcaacg ggccatcaca tggcaccact gggccttgcg tgcatgcagt acgcaacttt gcctaacctt aagaagccaa atggagcagc agcggaattg ccaccgcttc agttcaactg gaaagacgga tgtgcaccgg tgctcagccc acgtcagttg gagctgcact acacgaagca tcacaaggcg tatgttgaca agttgaatgc actggcggga gccacatacg atggaaaaac tatggaggat attatcgttg ctttagctaa tgattctgag aagaaggtct tgttcaacca agcggcacag cacttcaacc actcctttta ttggttgtgc attacaccca atgggaagcc aatgcccaaa tccctggagt ctgccctcac tgctcagttt ggttcagttg acggttttaa ggacacgttc atgcaagcgg gagcaaacaa ctttggctct ggctggacgt ggttgtgtgt ggacccgaaa aataagggta aacctcttgt aattgataat accagcaatg ccgggtgccc aatcacaaaa ggactccgac ccgtcttcac agtggatgtg tgggaacatg catattacaa ggactttgaa aaccggcgcg tggattacct caaggaaatt tggactattg ttgattggga gtttgtttca aggacgtatg aacaggctat gaagtaa
Details for TrbrA.01606.a.A1.GE34282
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 91
Validated AA Sequence
MRSVMMRCAT GRHMAPLGLA CMQYATLPNL KKPNGAAAEL PPLQFNWKDG CAPVLSPRQL ELHYTKHHKA YVDKLNALAG ATYDGKTMED IIVALANDSE KKVLFNQAAQ HFNHSFYWLC ITPNGKPMPK SLESALTAQF GSVDGFKDTF MQAGANNFGS GWTWLCVDPK NKGKPLVIDN TSNAGCPITE GLRPVFTVDV WEHAYYKDFE NRRVDYLKEI WAIVDWEFVS RTYEQAMK
Validated NT Sequence
attttnnnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt cgatgaggtc tgtcatgatg cgttgcgcaa cgggccgtca catggcacca ctgggccttg cgtgcatgca gtacgcaact ttgcctaacc ttaagaagcc aaatggagca gcagcggaat tgccaccgct tcagttcaac tggaaagacg gatgtgcacc ggtgctcagc ccacgtcagt tggagctgca ctacacgaag catcacaagg cgtatgttga caagttgaat gcactggcgg gagccacata cgatggaaaa actatggagg atattatcgt tgctttagct aatgattctg agaagaaggt cttgttcaac caagcggcac agcacttcaa ccactccttt tattggttgt gcattacacc caatgggaag ccaatgccca aatccctgga gtctgccctc actgctcagt ttggttcagt tgacggtttt aaggacacgt tcatgcaagc gggagcaaac aactttggct ctggctggac gtggttgtgt gtggacccga aaaataaggg taaacctctt gtaattgata ataccagcaa tgccgggtgc ccaatcacag aaggactccg acccgtcttc acagtggatg tgtgggaaca tgcatattac aaggactttg aaaaccggcg cgtggattac ctcaaggaaa tttgggctat tgttgattgg gagtttgttt caaggacgta tgaacaggct atgaagtaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccngctgct aacaaagccc gaaaggaagc tganttggct gctgnnnnnn ntnnnnnnna nnngnnnnaa gtnnnnnntn ntnnnnnncc cnnagntgan tngcnntcnn agnnnntnaa nnnatnnnnn tnnnnnnnnn nntnantnnn ncnannngnn nnnnnnnnna annnngnnnn cnncnnanna nnggnnnnnn c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRSVMMRCA TGHHMAPLGL ACMQYATLPN LKKPNGAAAE LPPLQFNWKD GCAPVLSPRQ LELHYTKHHK AYVDKLNALA GATYDGKTME DIIVALANDS EKKVLFNQAA QHFNHSFYWL CITPNGKPMP KSLESALTAQ FGSVDGFKDT FMQAGANNFG SGWTWLCVDP KNKGKPLVID NTSNAGCPIT KGLRPVFTVD VWEHAYYKDF ENRRVDYLKE IWTIVDWEFV SRTYEQAMK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaggtctgtc atgatgcgtt gcgcaacggg ccatcacatg gcaccactgg gccttgcgtg catgcagtac gcaactttgc ctaaccttaa gaagccaaat ggagcagcag cggaattgcc accgcttcag ttcaactgga aagacggatg tgcaccggtg ctcagcccac gtcagttgga gctgcactac acgaagcatc acaaggcgta tgttgacaag ttgaatgcac tggcgggagc cacatacgat ggaaaaacta tggaggatat tatcgttgct ttagctaatg attctgagaa gaaggtcttg ttcaaccaag cggcacagca cttcaaccac tccttttatt ggttgtgcat tacacccaat gggaagccaa tgcccaaatc cctggagtct gccctcactg ctcagtttgg ttcagttgac ggttttaagg acacgttcat gcaagcggga gcaaacaact ttggctctgg ctggacgtgg ttgtgtgtgg acccgaaaaa taagggtaaa cctcttgtaa ttgataatac cagcaatgcc gggtgcccaa tcacaaaagg actccgaccc gtcttcacag tggatgtgtg ggaacatgca tattacaagg actttgaaaa ccggcgcgtg gattacctca aggaaatttg gactattgtt gattgggagt ttgtttcaag gacgtatgaa caggctatga agtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa