TrbrA.01515.a

serine/threonine-protein kinase a, putative,protein kinase, putative (RDK2)

CENTER ID: TrbrA.01515.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.01515.a.B1.GE40767 full length 1 440
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.8.7110
RefSeq: XP_847541.1
UniProt: Q57YR8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAHKRATNG DNGLPVPPVD DDSEIEDPLE GILGSRTGCR YVKKKLLGQG SFGSVWRVEE TATGSIFAAK VMDTNNMSAK DRGFVINEVK CLSRCNNANI IRHHASFNRG GMLLIIAEYA DGGDLCRQIK VRQHAGRHFK EHEVLYIFLQ LCLALDHIHG NNMMHRDLKS ANVLLTTMGL VKLGDFGFSR QYEDSLSNPV GTTFCGTPYY LSPELWRRAP YSKKSEMWAL GVVLYEVMML KRPFGGRNMD ELINNIVHAR RAPLPNMYSD ELRRVCDQLL SLDPAKRPSL RQLFQQPFIR RGLDSLRRSV ETHKKIPPRT FSEIARNIDE VLRPGLPEYR GSSVTPHRGM LQRHTADRGW KDCELSLNAG GVSMRDVETG GVETVNLETL TSVCPVDAAT AQEKYVFALK NQTGKAYWFK DLAEDSYDYW ISALQKAISH
NT Sequence
atgaccgcgc acaaacgtgc aacaaacggg gataatggac tacccgtgcc gcctgtggac gatgactctg aaattgaaga tcctcttgaa ggcatccttg gctctcgcac cggttgcagg tatgtcaaaa aaaagctgtt gggtcaagga agttttggaa gcgtgtggcg ggtggaggaa acggcgacag ggtcgatctt tgcagcaaag gtgatggata caaacaatat gagcgcgaag gaccgtggtt ttgttataaa tgaggttaaa tgcctttcac gctgcaacaa cgcaaatatt atacgccacc atgcctcatt caaccgcggt gggatgctgc tcatcatcgc ggagtatgcc gacggtggcg acctctgcag gcaaatcaag gtgcggcagc acgcaggacg gcacttcaag gagcatgaag tgttatacat atttctgcaa ctatgtcttg cattggatca catccacggc aataacatga tgcaccgcga ccttaagtct gccaacgtac tgcttaccac aatgggcctt gtgaagttgg gtgactttgg cttcagtcgt caatacgagg actcgctgtc aaatcctgtt ggcactactt tctgtgggac gccatattat ttgagccccg aactctggcg ccgcgcgccg tacagcaaaa agagtgaaat gtgggctctt ggtgtggtgt tgtatgaagt gatgatgttg aagcgacctt ttggtggacg taatatggat gagctcatca acaatattgt ccatgcccgc cgcgccccgc ttccaaatat gtactcggat gaactgcgtc gagtgtgtga tcagttgctt tctctcgatc cagctaaacg gccgtcactg cgacagctct tccaacagcc tttcattcgc cgtggcttag actcactgcg acgaagtgtg gagacgcaca aaaaaattcc accaagaacc ttcagcgaga ttgcacgaaa cattgatgag gtgttgagac ctggtctccc ggagtatcgg gggagtagtg taacgcctca tcgtgggatg ttgcaacgtc acaccgcgga tcgtggttgg aaggattgcg agctttcact aaacgctggc ggtgtttcaa tgcgagatgt agaaactggt ggcgttgaaa cggtgaatct tgaaaccctc acatccgtct gtcccgtaga cgcggcaacc gctcaggaga aatatgtttt cgctcttaag aatcaaacag gaaaggcgta ttggtttaag gaccttgcag aggattcata cgattattgg atttccgccc ttcagaaagc catatcgcat tga
Details for TrbrA.01515.a.B1.GE40767
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMT AHKRATNGDN GLPVPPVDDD SEIEDPLEGI LGSRTGCRYV KKKLLGQGSF GSVWRVEETA TGSIFAAKVM DTNNMSAKDR GFVINEVKCL SRCNNANIIR HHASFNRGGM LLIIAEYADG GDLCRQIKVR QHAGWHFKEH EVLYIFLQLC LALDHIHGNN MMHRDLKSAN VLLTTMGLVK LGDFGFSRQY EDSLSNPVGT TFCGTPYYLS PELWRRAPYS KKSEMWALGV VLYEVMMLKR PFGGRNMDEL INNIVHARRA PLPNMYSDEL RRVCDQLLSL DPAKRPSLRQ LFQQPFIRRG LDSLRRSVET HKKIPPRTFS EIARNIDEVL RPGLPEYRGS SVTPHRGMLQ RHTADRGWKD CELSLNTGGV SMRDVETGGV ETVNLETLTS VCPVDAATAQ EKYVFALKNQ TGKAYWFKDL AEDSYDYWIS
Validated NT Sequence
ggcggaaatc caataatcgt atgaatcctc tgcaaggtcc ttaaaccaat acgcctttcc tgtttgattc ttaagagcga aaacatattt ctcctgagcg gttgccgcgt ctacgggaca gacggatgtg agggtttcaa gattcaccgt ttcaacgcca ccagtttcta catctcgcat tgaaacaccg ccagtgttta gtgaaagctc gcaatccttc caaccacgat ccgcggtgtg acgttgcaac atcccacgat gaggcgttac actactcccc cgatactccg ggagaccagg tctcaacacc tcatcaatgt ttcgtgcaat ctcgctgaag gttcttggtg gaattttttt gtgcgtctcc acacttcgtc gcagtgagtc taagccacgg cgaatgaaag gctgttggaa gagctgtcgc agtgacggcc gtttagctgg atcgagtgaa agcaactgat cacacactcg acgcagttca tccgagtaca tatttggaag cggggcgcgg cgggcatgga caatattgtt gatgagctca tccatattac gtccaccaaa aggtcgcttc aacatcatca cttcatacaa caccacacca agagcccaca tttcactctt tttgctgtac ggcgcgcggc gccagagttc ggggctcaaa taatatggcg tcccacagaa agtagtgcca acaggatttg acagcgagtc ctcgtattga cggctgaagc caaagtcacc caacttcaca aggcccattg tggtaagcag tacgttggca gacttaaggt cgcggtgcat catgttattg ccgtggatgt gatccaatgc aagacatagt tgcagaaata tgtataacac ttcatgctcc ttgaagtgcc atcctgcgtg ctgccgcacc ttgatttgcc tgcagaggtc gccaccgtcg gcatactccg cgatgatgag cagcatccca ccgcggttga atgaggcatg gtggcgtata atatttgcgt tgttgcagcg tgaaaggcat ttaacctcat ttataacaaa accacggtcc ttcgcgctca tattgtttgt atccatcacc tttgctgcaa agatcgaccc tgtcgccgtt tcctccaccc gccacacgct tccaaaactt ccttgaccca acagcttttt tttgacatac ctgcaaccgg tgcgagagcc aaggatgcct tcaagaggat cttcaatttc agagtcatcg tccacaggcg gcacgggtag tccattatcc ccgtttgttg cacgtttgtg cgcggtcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMT AHKRATNGDN GLPVPPVDDD SEIEDPLEGI LGSRTGCRYV KKKLLGQGSF GSVWRVEETA TGSIFAAKVM DTNNMSAKDR GFVINEVKCL SRCNNANIIR HHASFNRGGM LLIIAEYADG GDLCRQIKVR QHAGRHFKEH EVLYIFLQLC LALDHIHGNN MMHRDLKSAN VLLTTMGLVK LGDFGFSRQY EDSLSNPVGT TFCGTPYYLS PELWRRAPYS KKSEMWALGV VLYEVMMLKR PFGGRNMDEL INNIVHARRA PLPNMYSDEL RRVCDQLLSL DPAKRPSLRQ LFQQPFIRRG LDSLRRSVET HKKIPPRTFS EIARNIDEVL RPGLPEYRGS SVTPHRGMLQ RHTADRGWKD CELSLNAGGV SMRDVETGGV ETVNLETLTS VCPVDAATAQ EKYVFALKNQ TGKAYWFKDL AEDSYDYWIS ALQKAISH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg cgcacaaacg tgcaacaaac ggggataatg gactacccgt gccgcctgtg gacgatgact ctgaaattga agatcctctt gaaggcatcc ttggctctcg caccggttgc aggtatgtca aaaaaaagct gttgggtcaa ggaagttttg gaagcgtgtg gcgggtggag gaaacggcga cagggtcgat ctttgcagca aaggtgatgg atacaaacaa tatgagcgcg aaggaccgtg gttttgttat aaatgaggtt aaatgccttt cacgctgcaa caacgcaaat attatacgcc accatgcctc attcaaccgc ggtgggatgc tgctcatcat cgcggagtat gccgacggtg gcgacctctg caggcaaatc aaggtgcggc agcacgcagg acggcacttc aaggagcatg aagtgttata catatttctg caactatgtc ttgcattgga tcacatccac ggcaataaca tgatgcaccg cgaccttaag tctgccaacg tactgcttac cacaatgggc cttgtgaagt tgggtgactt tggcttcagt cgtcaatacg aggactcgct gtcaaatcct gttggcacta ctttctgtgg gacgccatat tatttgagcc ccgaactctg gcgccgcgcg ccgtacagca aaaagagtga aatgtgggct cttggtgtgg tgttgtatga agtgatgatg ttgaagcgac cttttggtgg acgtaatatg gatgagctca tcaacaatat tgtccatgcc cgccgcgccc cgcttccaaa tatgtactcg gatgaactgc gtcgagtgtg tgatcagttg ctttctctcg atccagctaa acggccgtca ctgcgacagc tcttccaaca gcctttcatt cgccgtggct tagactcact gcgacgaagt gtggagacgc acaaaaaaat tccaccaaga accttcagcg agattgcacg aaacattgat gaggtgttga gacctggtct cccggagtat cgggggagta gtgtaacgcc tcatcgtggg atgttgcaac gtcacaccgc ggatcgtggt tggaaggatt gcgagctttc actaaacgct ggcggtgttt caatgcgaga tgtagaaact ggtggcgttg aaacggtgaa tcttgaaacc ctcacatccg tctgtcccgt agacgcggca accgctcagg agaaatatgt tttcgctctt aagaatcaaa caggaaaggc gtattggttt aaggaccttg cagaggattc atacgattat tggatttccg cccttcagaa agccatatcg cattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat