TrbrA.01488.a

Aurora kinase 2, putative (AUK2)

CENTER ID: TrbrA.01488.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.01488.a.A1.GE34280 full length 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.3.3920
RefSeq: XP_843984.1
UniProt: Q57WC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVIWFSAGDG VGFPTPTPWE IQPPRGLTSA DGCRGGLRDG GEPAVISRQQ RQQKPSRHQQ QHHCVRQPKP KVVIQQSDLR DEQFQRLSVL GEGSYSVVVA ARHLPSRQVV ALKELSRQRL RDAKLETQLQ WEINLHRTLR HPNIVRLLSY YITPRSVVLV LELCRGGSLL RRLQATAERR FDEGRATRYI RHVAQALAYL HEHGIVHRDL KPGNILVDAR GVARLADFGW SKGLAGSAAA AVITNGYDAS EVAPRCAAGE TDACSETEDG HGRLTVCGTL DYMPPELLGG TPCSYKADMW SLGALLVEIL SGQPPFYRTS QQETLQAIQD EGPQLDVDGS VLSPLARDLA LQLLQKDPNV RPTAAEVLQH PWLRGKRPRV
NT Sequence
atggttattt ggttttccgc tggtgatggt gtgggctttc ccaccccaac accatgggaa attcaacccc cacggggctt aacctccgct gatggttgcc gaggtggcct gagggatggt ggggagccgg ctgtaatctc ccgtcaacag cgacaacaaa agccttctcg gcatcaacaa cagcatcatt gtgtgagaca gccgaaaccg aaggttgtga tccagcagag tgatctgcgt gatgagcaat tccagcggct gagcgtgctg ggggagggtt cgtactctgt tgtggttgcc gcccggcacc tcccttcacg tcaagtcgtt gcgctgaaag aactgtcgcg acagcggctc cgtgatgcta agttagagac tcagctgcag tgggagatca atctccaccg aactttgcgg caccccaata ttgtgcgttt gctcagctat tacataactc cgcgaagtgt cgtgcttgtg ctggaactat gtaggggtgg gtcgctgctg cggaggcttc aggcgacggc ggagagacga tttgacgagg gccgggcgac acgttatatt cgacatgttg ctcaggcact ggcatatctt cacgaacacg gcattgttca ccgtgaccta aagccgggaa atattctcgt cgacgccaga ggtgtagcac ggcttgctga cttcggctgg agtaagggtc ttgccggctc tgcagccgct gctgtgataa caaacgggta tgatgcgtct gaagtggcgc cacgctgcgc ggcgggtgaa accgacgcat gtagtgagac ggaggacggc cacgggaggc tgacagtgtg tgggacgctt gattatatgc caccagaact tttgggcggc acgccatgct cctacaaggc tgatatgtgg tcgctgggtg cactcttggt ggaaatactg tccggacaac cgccctttta ccgcacgtct cagcaggaga cgttacaggc tattcaagac gagggcccgc agctagatgt tgatggtagt gtcctttcac cacttgcccg tgatctcgcc ttgcagttgc tgcagaaaga ccccaacgtg cgcccgaccg ctgccgaggt gctgcagcat ccgtggttac ggggaaaacg tcctcgcgtg tga
Details for TrbrA.01488.a.A1.GE34280
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 75
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVIWFSAGD GVGFPTPTPW EIQPPRGLTS ADGCRGGLRD GGEPAVISRQ QRQQKPSRHQ QEHHCVRQPK PKVVIQQSDL RDEQFQRLSV LGEGSYSVVV AARHLPSRQV VALKELSRQR LRDAKLETQL QWEINLHRTL RHPNIVRLLS YYITPRSVVL VLELCRGGSL LRRLQATAER RFDEGRATRY IRHVAQALAY LHEHGIVHRD LKPGNILVDA RXVARLADFG WSKGLAGSAA AAVITNGYXA SEVAPRCAAG ETDACSETEX XXRXADSVWX A
Validated NT Sequence
annnattttn ttnnntttaa gaaggagann taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gttatttggt tttccgctgg tgatggtgtg ggctttccca ccccaacacc atgggaaatt caacccccac ggggcttaac ctccgctgat ggttgccgag gtggcctgag ggatggtggg gagccggctg taatctcccg tcaacagcga caacaaaagc cttctcggca tcaacaagag catcattgtg tgagacagcc gaaaccgaag gttgtgatcc agcagagtga tctgcgtgat gagcaattcc agcggctgag cgtgctgggg gagggttcgt actctgttgt ggttgccgcc cggcacctcc cttcacgtca agtcgttgcg ctgaaagaac tgtcgcgaca gcggctccgt gacgctaagt tagagactca gctgcagtgg gagatcaatc tccaccgaac tttgcggcac cccaatattg tgcgtttgct cagctattac ataactccgc gaagtgtcgt gcttgtgctg gaactatgta ggggtgggtc gctgctgcgg aggcttcagg cgacggcgga gagacgattt gacgagggcc gggcgacacg ttatattcga catgttgctc aggcactggc atatcttcac gaacacggca ttgttcaccg tgacctaaag ccgggaaata ttctcgtcga cgccagangt gtagcacggc ttgctgactt cggctggagt aagggtcttg ccggctctgc agccgctgct gtgataacaa acgggtatga ngcgtctgaa gtggcgccac gctgcgcggc gggtgaaacc gacgcatgta gtgagacnga gnacngncnn cgggnngctg acagtgtgtg ggangcttga ttatntgcna ccagaacttt tgggcnncac gccatgctcn anangctgan atgnngnnnc tgggngcact ntnnnggnan nnctgnnnna nacnttttac ngcnngnntc annngnannn gnnacnngct nntnnnanna ngnnncnncn nntagannnn nnnnnnnnnn nnttnnnccn nnnnccnnnn cnnnnnnnnn nnnnnnnncc cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIWFSAGD GVGFPTPTPW EIQPPRGLTS ADGCRGGLRD GGEPAVISRQ QRQQKPSRHQ QQHHCVRQPK PKVVIQQSDL RDEQFQRLSV LGEGSYSVVV AARHLPSRQV VALKELSRQR LRDAKLETQL QWEINLHRTL RHPNIVRLLS YYITPRSVVL VLELCRGGSL LRRLQATAER RFDEGRATRY IRHVAQALAY LHEHGIVHRD LKPGNILVDA RGVARLADFG WSKGLAGSAA AAVITNGYDA SEVAPRCAAG ETDACSETED GHGRLTVCGT LDYMPPELLG GTPCSYKADM WSLGALLVEI LSGQPPFYRT SQQETLQAIQ DEGPQLDVDG SVLSPLARDL ALQLLQKDPN VRPTAAEVLQ HPWLRGKRPR V
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttatttgg ttttccgctg gtgatggtgt gggctttccc accccaacac catgggaaat tcaaccccca cggggcttaa cctccgctga tggttgccga ggtggcctga gggatggtgg ggagccggct gtaatctccc gtcaacagcg acaacaaaag ccttctcggc atcaacaaca gcatcattgt gtgagacagc cgaaaccgaa ggttgtgatc cagcagagtg atctgcgtga tgagcaattc cagcggctga gcgtgctggg ggagggttcg tactctgttg tggttgccgc ccggcacctc ccttcacgtc aagtcgttgc gctgaaagaa ctgtcgcgac agcggctccg tgatgctaag ttagagactc agctgcagtg ggagatcaat ctccaccgaa ctttgcggca ccccaatatt gtgcgtttgc tcagctatta cataactccg cgaagtgtcg tgcttgtgct ggaactatgt aggggtgggt cgctgctgcg gaggcttcag gcgacggcgg agagacgatt tgacgagggc cgggcgacac gttatattcg acatgttgct caggcactgg catatcttca cgaacacggc attgttcacc gtgacctaaa gccgggaaat attctcgtcg acgccagagg tgtagcacgg cttgctgact tcggctggag taagggtctt gccggctctg cagccgctgc tgtgataaca aacgggtatg atgcgtctga agtggcgcca cgctgcgcgg cgggtgaaac cgacgcatgt agtgagacgg aggacggcca cgggaggctg acagtgtgtg ggacgcttga ttatatgcca ccagaacttt tgggcggcac gccatgctcc tacaaggctg atatgtggtc gctgggtgca ctcttggtgg aaatactgtc cggacaaccg cccttttacc gcacgtctca gcaggagacg ttacaggcta ttcaagacga gggcccgcag ctagatgttg atggtagtgt cctttcacca cttgcccgtg atctcgcctt gcagttgctg cagaaagacc ccaacgtgcg cccgaccgct gccgaggtgc tgcagcatcc gtggttacgg ggaaaacgtc ctcgcgtgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a