TrbrA.01480.a

serine/threonine-protein kinase, putative (AGC essential kinase 1) (AEK1)

CENTER ID: TrbrA.01480.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.01480.a.B3.GE40766 putative kinase domain 57 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.3.2440
RefSeq: XP_843838.1
UniProt: Q582V7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNRLFGRGK SKKDKDKDKD KSKEKEKRDG ESSNTKSGNS HKSGSERKDE NTTEKVTKED FETIDTLGKG SFAYVVLVRR VGTNNLYAMK VVNKQGLLDH NRCRDVFIER NVLSRINHPF LLKLYWTFQS EHKLFFVMEY MAGGDLDKYM NSVPNKQLDL PTSKLYGAEI LMAILTLHEQ SVIYRDLKPE NILLTGDGHC VLADFGLSKD FYDAKMGENA SVTDMRANSF VGSPFYVAPD VLKQREYTNA VDFWSFGILL YRMLCGRTPF SGKNMKEVFD NILYSDLRFP STVSIPSEAK DLISRLLVKD AAHRIKGPEV KAHPFWTGIN FDEVMQKKVK PPRWVPAPSV DKLIAERSAA QSGNNSSKNP AQVVNTPAHS SQLNARQQQL FNGFSCTTDN HLGGGS
NT Sequence
atgctcaata gactatttgg ccggggcaag agcaagaagg ataaagataa ggataaggat aaatcaaagg aaaaggaaaa gagggatggc gaaagctcca atacaaagag tggaaacagt cacaagagtg gcagcgagcg taaggatgaa aataccaccg aaaaggtgac aaaggaggac tttgaaacaa ttgacacatt aggaaaggga tccttcgcat atgtagttct tgtgcgccgt gtggggacaa acaacttata tgctatgaag gtggtaaata aacaggggct tctcgatcac aatcgatgcc gcgatgtctt tatcgaacgt aatgtgctct cccgtattaa tcatcccttc ctcttaaagc tctactggac ttttcagtcc gagcacaaac tcttctttgt gatggagtac atggcgggag gtgaccttga caaatatatg aattcagttc ctaacaagca gcttgactta cccacatcca agttgtatgg ggctgagatc ctaatggcaa ttctcacatt gcacgaacag agcgtcattt atcgtgattt gaaaccggag aacatattgt taactggaga tggacactgc gttttggcgg attttggtct atcaaaagac ttttatgatg ccaaaatggg tgagaatgca tcggtaacgg acatgcgggc gaactcattt gttggttcac cattttatgt cgcacctgat gttctgaagc aacgtgagta cacgaacgcc gtggactttt ggtcctttgg aattctgtta taccgcatgt tatgtggccg cacgccattt agtgggaaga atatgaaaga agtgttcgac aacattctct attcggatct ccgcttccca agcactgttt caattccgtc tgaggcgaaa gatttgataa gtcggctgct ggtgaaagat gcagcccatc gcattaaggg accagaagta aaagctcacc cattctggac aggcattaac ttcgatgaag tgatgcaaaa gaaggtgaaa ccaccgaggt gggtacccgc tccatcagta gataagttaa tagcggaacg ctcagctgca caaagtggaa acaactcatc aaagaatccc gcacaggttg ttaacacacc agcacattcg agtcaattaa atgctcggca gcagcaactc tttaacgggt tttcatgtac tactgataat catctgggtg gtggtagtta g
Details for TrbrA.01480.a.B3.GE40766
HARVESTED ON: data unavailable
SEQUENCED ON: 11/22/2016
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT KEDFETIDTL GKGSFAYVVL VRRVGTNNLY AMKVVNKQGL LDHNRCRDVF IERNVLSRIN HPFLLKLYWT FQSEHKLFFV MEYMAGGDLD KYMNSVPNKQ LDLPTSKLYG AEILMAILTL HEQSVIYRDL KPENILLTGD GHCVLADFGL SKDFYDAKMG ENASVTDMRA NSFVGSPFYV APDVLKQREY TNAVDFWSFG ILLYRMLCGR TPFSGKNMKE VFDNILYSDL RFPSTVSIPS EAKDLISRLL VKDAAHRIKG PEVKAHPFWT GINFDEVMQ
Validated NT Sequence
atcttactca cttattgcat cacttcatcg aagttaatgc ctgtccagaa tgggtgagct tttacttctg gtcccttaat gcgatgggct gcatctttca ccagcagccg acttatcaaa tctttcgcct cagacggaat tgaaacagtg cttgggaagc ggagatccga atagagaatg ttgtcgaaca cttctttcat attcttccca ctaaatggcg tgcggccaca taacatgcgg tataacagaa ttccaaagga ccaaaagtcc acggcgttcg tgtactcacg ttgcttcaga acatcaggtg cgacataaaa tggtgaacca acaaatgagt tcgcccgcat gtccgttacc gatgcattct cacccatttt ggcatcataa aagtcttttg atagaccaaa atccgccaaa acgcagtgtc catctccagt taacaatatg ttctccggtt tcaaatcacg ataaatgacg ctctgttcgt gcaatgtgag aattgccatt aggatctcag ccccatacaa cttggatgtg ggtaagtcaa gctgcttgtt aggaactgaa ttcatatatt tgtcaaggtc acctcccgcc atgtactcca tcacaaagaa gagtttgtgc tcggactgaa aagtccagta gagctttaag aggaagggat gattaatacg ggagagcaca ttacgttcga taaagacatc gcggcatcga ttgtgatcga gaagcccctg tttatttacc accttcatag catataagtt gtttgtcccc acacggcgca caagaactac atatgcgaag gatccttttc ctaatgtgtc aattgtttca aagtcctcct ttgtcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHTK EDFETIDTLG KGSFAYVVLV RRVGTNNLYA MKVVNKQGLL DHNRCRDVFI ERNVLSRINH PFLLKLYWTF QSEHKLFFVM EYMAGGDLDK YMNSVPNKQL DLPTSKLYGA EILMAILTLH EQSVIYRDLK PENILLTGDG HCVLADFGLS KDFYDAKMGE NASVTDMRAN SFVGSPFYVA PDVLKQREYT NAVDFWSFGI LLYRMLCGRT PFSGKNMKEV FDNILYSDLR FPSTVSIPSE AKDLISRLLV KDAAHRIKGP EVKAHPFWTG INFDEVMQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacaaagg aggactttga aacaattgac acattaggaa agggatcctt cgcatatgta gttcttgtgc gccgtgtggg gacaaacaac ttatatgcta tgaaggtggt aaataaacag gggcttctcg atcacaatcg atgccgcgat gtctttatcg aacgtaatgt gctctcccgt attaatcatc ccttcctctt aaagctctac tggacttttc agtccgagca caaactcttc tttgtgatgg agtacatggc gggaggtgac cttgacaaat atatgaattc agttcctaac aagcagcttg acttacccac atccaagttg tatggggctg agatcctaat ggcaattctc acattgcacg aacagagcgt catttatcgt gatttgaaac cggagaacat attgttaact ggagatggac actgcgtttt ggcggatttt ggtctatcaa aagactttta tgatgccaaa atgggtgaga atgcatcggt aacggacatg cgggcgaact catttgttgg ttcaccattt tatgtcgcac ctgatgttct gaagcaacgt gagtacacga acgccgtgga cttttggtcc tttggaattc tgttataccg catgttatgt ggccgcacgc catttagtgg gaagaatatg aaagaagtgt tcgacaacat tctctattcg gatctccgct tcccaagcac tgtttcaatt ccgtctgagg cgaaagattt gataagtcgg ctgctggtga aagatgcagc ccatcgcatt aagggaccag aagtaaaagc tcacccattc tggacaggca ttaacttcga tgaagtgatg caatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat