TrbrA.01471.a
aspartate aminotransferase, putative
CENTER ID:
TrbrA.01471.a
ORGANISM:
Trypanosoma brucei TREU927
ASSOCIATED DISEASE:
African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
unclassified
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
TrbrA.01471.a.A1.GE34283
full length
info
1
388
TrbrA.01471.a.A1.GE34283
TrbrA.01471.a.B1.GE36914
full length
info
1
388
TrbrA.01471.a.B1.GE36914
TrbrA.01471.a.B11.GE36562
full length
info
1
388
TrbrA.01471.a.B11.GE36562
TrbrA.01471.a.B12.GE36563
full length
info
1
388
TrbrA.01471.a.B12.GE36563
TrbrA.01471.a.B13.GE36564
full length
info
1
388
TrbrA.01471.a.B13.GE36564
TrbrA.01471.a.B15.GE36566
full length
info
1
388
TrbrA.01471.a.B15.GE36566
TrbrA.01471.a.B16.GE36567
full length
info
1
388
TrbrA.01471.a.B16.GE36567
TrbrA.01471.a.B17.GE36568
full length
info
1
388
TrbrA.01471.a.B17.GE36568
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
TrbrA.01471.a.B11.PS01759
full length
info
1
388
PS01759
TrbrA.01471.a.B13.PS01760
full length
info
1
388
PS01760
TrbrA.01471.a.A1.PW34891
full length
info
1
388
PW34891
TrbrA.01471.a.B1.PW37325
full length
info
1
388
PW37325
Structures
4EU1
DEPOSITED: 4/25/2012
DETERMINATION: XRay
CLONE: TrbrA.01471.a.A1.GE34283
PROTEIN: TrbrA.01471.a.A1.PW34891
4W5K
DEPOSITED: 8/18/2014
DETERMINATION: XRay
CLONE: TrbrA.01471.a.B13.GE36564
PROTEIN: TrbrA.01471.a.B13.PS01760
Publications by SSGCID
Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia.
Abendroth J, Choi R, Clifton MC, Edwards TE, Lorimer DD, Lukacs CM, Myler P, Staker BL, van Voorhis W, Wall A
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 566-71
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKPDPILGL GQDFRMDPAK RKVNLSIGVY RDDADQPFVL ECVKQATLGT NMDYAPVTGI ASFVEEAQKL CFGPTCAALR DGRIASCQTL GGTGALRIGG DLLNRFVANC NRIYGPDVGY PNHESIFAKA GMELTPYSYY DPATKGFNLA GMLECLDKAP EGSVILVHAC AHNPTGVDPT HDDWRQVCDV IKRRNHIPFV DMAYQGFATG QLDYDAFVPR HLVDMVPNLI VAQSFSKNFG LYGHRCGALH ISTASAEEAK RLVSQLALLI RPMYSNPPLY GAWVVSSILK DPQLTALWKK ELKQMSSRIA EVRKRLVSEL KACGSVHDWS HIERQVGMMA YTGLTREQVE LLRSEYHIYM TLNGRAAVSG LNSTNVEYVS QAIHNVTK
NT Sequence
atggggaaac cggatcccat cctcggcctc gggcaggatt ttcgtatgga tcccgccaaa aggaaggtga atttgtctat tggtgtgtac cgcgacgacg ccgaccagcc ttttgtgctg gaatgtgtga aacaagccac attaggcacc aacatggact acgccccagt cacaggaatt gcaagttttg tagaggaggc tcaaaaactg tgttttggcc caacgtgtgc ggcgcttcgg gatggacgca ttgcaagttg ccaaacgctt ggcggcacag gtgcgcttcg gattggtggt gaccttctca atcgctttgt ggccaattgt aaccgcattt acggtcctga cgtcggttat ccgaatcatg agagtatctt tgctaaggcg gggatggaac ttaccccata ttcgtactat gatcctgcca caaaaggttt taaccttgcg ggtatgctcg agtgcctaga caaggcgcct gagggctctg ttatactcgt tcatgcctgc gcacacaatc caactggcgt cgatccaaca cacgatgact ggcgacaggt gtgcgatgtg ataaagaggc gtaaccatat tccgttcgta gacatggctt accaaggttt tgctactggt caattggatt atgatgcctt cgtgccacgc cacctggtgg atatggtgcc aaacctgatt gttgcgcaga gtttctccaa gaactttggc ctgtacgggc atcgctgtgg tgctcttcac atctcgacgg cgagtgctga agaggcgaag cgtttggtct cacaattggc cctactgatt cggcccatgt atagcaatcc gcctctttat ggtgcctggg tcgtaagttc tattctaaag gacccgcagc tgactgcact ctggaagaag gaattaaagc aaatgtcatc tcgcattgct gaggtgcgca agcgtttggt ttcagagctg aaagcatgcg gctccgtcca tgactggtct cacattgagc gccaagttgg tatgatggca tacacaggcc taacaaggga gcaggtggag ttactgagga gcgagtacca catatacatg acgttgaatg gtcgcgctgc ggtgtccgga cttaacagca caaatgttga atatgtttcc caagctattc acaacgttac taaatga
Details for TrbrA.01471.a.A1.GE34283
HARVESTED ON:
12/19/2011
SEQUENCED ON:
12/28/2011
EXPECTED MW:
45kDa
OBSERVED MW:
45kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
96
PERCENT COVERAGE:
62
Details for TrbrA.01471.a.B1.GE36914
HARVESTED ON:
5/8/2013
SEQUENCED ON:
5/8/2013
EXPECTED MW:
44kDa
OBSERVED MW:
44kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with sequence variation
PERCENT IDENTITY:
98
PERCENT COVERAGE:
77
Details for TrbrA.01471.a.B11.GE36562
HARVESTED ON:
1/9/2013
SEQUENCED ON:
1/8/2013
EXPECTED MW:
44kDa
OBSERVED MW:
43kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
94
PERCENT COVERAGE:
76
Details for TrbrA.01471.a.B12.GE36563
HARVESTED ON:
1/9/2013
SEQUENCED ON:
1/7/2013
EXPECTED MW:
44kDa
OBSERVED MW:
43kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
98
PERCENT COVERAGE:
78
Details for TrbrA.01471.a.B13.GE36564
HARVESTED ON:
1/9/2013
SEQUENCED ON:
1/7/2013
EXPECTED MW:
44kDa
OBSERVED MW:
43kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Few (1-10)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with sequence variation
PERCENT IDENTITY:
95
PERCENT COVERAGE:
76
Details for TrbrA.01471.a.B15.GE36566
HARVESTED ON:
1/9/2013
SEQUENCED ON:
1/7/2013
EXPECTED MW:
44kDa
OBSERVED MW:
43kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
99
PERCENT COVERAGE:
91
Details for TrbrA.01471.a.B16.GE36567
HARVESTED ON:
1/9/2013
SEQUENCED ON:
1/7/2013
EXPECTED MW:
44kDa
OBSERVED MW:
43kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
79
PERCENT COVERAGE:
99
Details for TrbrA.01471.a.B17.GE36568
HARVESTED ON:
1/9/2013
SEQUENCED ON:
1/7/2013
EXPECTED MW:
44kDa
OBSERVED MW:
43kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with sequence variation
PERCENT IDENTITY:
95
PERCENT COVERAGE:
82
Details for TrbrA.01471.a.B11.PS01759
PURIFICATION DATe:
2/27/2013
CONCENTRATION:
29mg/ml
OBSERVED MW:
39kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
5
VIAL VOLUME:
200µl
PERCENT IDENTITY:
94
PERCENT COVERAGE:
76
Details for TrbrA.01471.a.B13.PS01760
PURIFICATION DATe:
2/27/2013
CONCENTRATION:
28.8mg/ml
OBSERVED MW:
39kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
7
VIAL VOLUME:
200µl
PERCENT IDENTITY:
95
PERCENT COVERAGE:
76
Details for TrbrA.01471.a.A1.PW34891
PURIFICATION DATe:
2/17/2012
CONCENTRATION:
27.7mg/ml
OBSERVED MW:
38kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
8
VIAL VOLUME:
110µl
PERCENT IDENTITY:
96
PERCENT COVERAGE:
62
Details for TrbrA.01471.a.B1.PW37325
PURIFICATION DATe:
7/8/2013
CONCENTRATION:
20mg/ml
OBSERVED MW:
43kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
8
VIAL VOLUME:
200µl
PERCENT IDENTITY:
98
PERCENT COVERAGE:
77