TrbrA.01471.a

aspartate aminotransferase, putative

CENTER ID: TrbrA.01471.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TrbrA.01471.a.A1.GE34283 full length 1 388
TrbrA.01471.a.B1.GE36914 full length 1 388
TrbrA.01471.a.B11.GE36562 full length 1 388
TrbrA.01471.a.B12.GE36563 full length 1 388
TrbrA.01471.a.B13.GE36564 full length 1 388
TrbrA.01471.a.B15.GE36566 full length 1 388
TrbrA.01471.a.B16.GE36567 full length 1 388
TrbrA.01471.a.B17.GE36568 full length 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TrbrA.01471.a.B11.PS01759 full length 1 388
TrbrA.01471.a.B13.PS01760 full length 1 388
TrbrA.01471.a.A1.PW34891 full length 1 388
TrbrA.01471.a.B1.PW37325 full length 1 388
Structures
4EU1
DEPOSITED: 4/25/2012
DETERMINATION: XRay
CLONE: TrbrA.01471.a.A1.GE34283
PROTEIN: TrbrA.01471.a.A1.PW34891
4W5K
DEPOSITED: 8/18/2014
DETERMINATION: XRay
CLONE: TrbrA.01471.a.B13.GE36564
PROTEIN: TrbrA.01471.a.B13.PS01760
Publications by SSGCID
Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia.
Abendroth J, Choi R, Clifton MC, Edwards TE, Lorimer DD, Lukacs CM, Myler P, Staker BL, van Voorhis W, Wall A
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 566-71
PMID: 25945710; PMCID: PMC4427166
PDB: 3MEB, 4EU1, 4H51, 4W5K
External Resources
RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb11.02.2740
OrthoMCL: OG5_126737
RefSeq: XP_828584.1
UniProt: Q385Q9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKPDPILGL GQDFRMDPAK RKVNLSIGVY RDDADQPFVL ECVKQATLGT NMDYAPVTGI ASFVEEAQKL CFGPTCAALR DGRIASCQTL GGTGALRIGG DLLNRFVANC NRIYGPDVGY PNHESIFAKA GMELTPYSYY DPATKGFNLA GMLECLDKAP EGSVILVHAC AHNPTGVDPT HDDWRQVCDV IKRRNHIPFV DMAYQGFATG QLDYDAFVPR HLVDMVPNLI VAQSFSKNFG LYGHRCGALH ISTASAEEAK RLVSQLALLI RPMYSNPPLY GAWVVSSILK DPQLTALWKK ELKQMSSRIA EVRKRLVSEL KACGSVHDWS HIERQVGMMA YTGLTREQVE LLRSEYHIYM TLNGRAAVSG LNSTNVEYVS QAIHNVTK
NT Sequence
atggggaaac cggatcccat cctcggcctc gggcaggatt ttcgtatgga tcccgccaaa aggaaggtga atttgtctat tggtgtgtac cgcgacgacg ccgaccagcc ttttgtgctg gaatgtgtga aacaagccac attaggcacc aacatggact acgccccagt cacaggaatt gcaagttttg tagaggaggc tcaaaaactg tgttttggcc caacgtgtgc ggcgcttcgg gatggacgca ttgcaagttg ccaaacgctt ggcggcacag gtgcgcttcg gattggtggt gaccttctca atcgctttgt ggccaattgt aaccgcattt acggtcctga cgtcggttat ccgaatcatg agagtatctt tgctaaggcg gggatggaac ttaccccata ttcgtactat gatcctgcca caaaaggttt taaccttgcg ggtatgctcg agtgcctaga caaggcgcct gagggctctg ttatactcgt tcatgcctgc gcacacaatc caactggcgt cgatccaaca cacgatgact ggcgacaggt gtgcgatgtg ataaagaggc gtaaccatat tccgttcgta gacatggctt accaaggttt tgctactggt caattggatt atgatgcctt cgtgccacgc cacctggtgg atatggtgcc aaacctgatt gttgcgcaga gtttctccaa gaactttggc ctgtacgggc atcgctgtgg tgctcttcac atctcgacgg cgagtgctga agaggcgaag cgtttggtct cacaattggc cctactgatt cggcccatgt atagcaatcc gcctctttat ggtgcctggg tcgtaagttc tattctaaag gacccgcagc tgactgcact ctggaagaag gaattaaagc aaatgtcatc tcgcattgct gaggtgcgca agcgtttggt ttcagagctg aaagcatgcg gctccgtcca tgactggtct cacattgagc gccaagttgg tatgatggca tacacaggcc taacaaggga gcaggtggag ttactgagga gcgagtacca catatacatg acgttgaatg gtcgcgctgc ggtgtccgga cttaacagca caaatgttga atatgtttcc caagctattc acaacgttac taaatga
Details for TrbrA.01471.a.A1.GE34283
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 62
Validated AA Sequence
MGKPDPILGL GQDFRMDPAK RKVNLSIGVY RDDADQPFVL ECVKQATLGT NMDYAPVTGI ASFVEEAQKL CFGPTCAALR DGRIASCQTL GGTGALRIGG DLLNRFVANC NRIYGPDVGY PNHESIFAKA GMELTPYSYY DPATKGLNLA GMLECLDKAP EGSVILVHAC AHNPTGVDPT HDDWRQVCDV IKRRNHIPFV DMAYQGFATG QLDYDAFVPR HLVDMVPNLI VAQSFSKNFX PVRASLWCSS HLNGEC
Validated NT Sequence
gtaacgttgt gaatagcttg tgaaacatat tcaacatttg tgctgttaag tccggacacc gcagcgcgac catttaatgt catgtatatg tggtactcgc tcctcagtaa ctccacctgc tcccttgtta ggcctgtgta tgccatcata ccaacttggc gctcaatgtg agaccagtca tggacggagc cgcatgcttt cagctctgaa accaaacgct tgcgcacctc agcaatgcga gatgacattt gcttcaattc cttcttccag agtgcagtca gctgcgggtc ctttagaata gaacttacga cccaggcacc ataaagaggc ggattgttat acatgggccg aatcagtagg gccaattgtg agaccaaacg cttcgcctct tcagcactcg ccgttgagat gtgaagagca ccacagcgat gcccgtacag gccnaaagtt cttggagaaa ctctgcgcaa caatcaggtt tggcaccata tccaccaggt ggcgtggcac gaaggcatca taatccaatt gaccagtagc aaaaccttgg taagccatgt ctacgaacgg aatatggtta cgcctcttta tcacatcgca cacctgtcgc cagtcatcgt gtgttggatc gacgccagtt ggattgtgtg cgcaggcatg aacgagtata acagagccct caggcgcctt gtctaggcac tcgagcatac ccgcaaggtt taagcctttt gtggcaggat catagtacga atatggggta agttccattc ccgccttagc aaagatactc tcatgattcg gataaccgac gtcaggaccg taaatgcggt tacaattggc cacaaagcga ttgagaaggt caccaccaat ccgaagcgca cctgtgccgc caagcgtttg gcaacttgca atgcgtccat cccgaagcgc cgcacacgtt gggccaaaac acagtttttg agcctcctct acaaaacttg caattcctgt gactggggcg tagtccatgt tggtgcctaa tgtggcttgt ttcacacatt ccagcacaaa aggctggtcg gcgtcgtcgc ggtacacacc aatagacaaa ttcaccttcc ttttggcggg atccatacga aaatcctgcc cgaggccgag gatgggatcc ggtttcccca tcgaaccagg accctgggtc tgagcttcca gggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGKPDPILG LGQDFRMDPA KRKVNLSIGV YRDDADQPFV LECVKQATLG TNMDYAPVTG IASFVEEAQK LCFGPTCAAL RDGRIASCQT LGGTGALRIG GDLLNRFVAN CNRIYGPDVG YPNHESIFAK AGMELTPYSY YDPATKGFNL AGMLECLDKA PEGSVILVHA CAHNPTGVDP THDDWRQVCD VIKRRNHIPF VDMAYQGFAT GQLDYDAFVP RHLVDMVPNL IVAQSFSKNF GLYGHRCGAL HISTASAEEA KRLVSQLALL IRPMYSNPPL YGAWVVSSIL KDPQLTALWK KELKQMSSRI AEVRKRLVSE LKACGSVHDW SHIERQVGMM AYTGLTREQV ELLRSEYHIY MTLNGRAAVS GLNSTNVEYV SQAIHNVTK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggggaaaccg gatcccatcc tcggcctcgg gcaggatttt cgtatggatc ccgccaaaag gaaggtgaat ttgtctattg gtgtgtaccg cgacgacgcc gaccagcctt ttgtgctgga atgtgtgaaa caagccacat taggcaccaa catggactac gccccagtca caggaattgc aagttttgta gaggaggctc aaaaactgtg ttttggccca acgtgtgcgg cgcttcggga tggacgcatt gcaagttgcc aaacgcttgg cggcacaggt gcgcttcgga ttggtggtga ccttctcaat cgctttgtgg ccaattgtaa ccgcatttac ggtcctgacg tcggttatcc gaatcatgag agtatctttg ctaaggcggg gatggaactt accccatatt cgtactatga tcctgccaca aaaggtttta accttgcggg tatgctcgag tgcctagaca aggcgcctga gggctctgtt atactcgttc atgcctgcgc acacaatcca actggcgtcg atccaacaca cgatgactgg cgacaggtgt gcgatgtgat aaagaggcgt aaccatattc cgttcgtaga catggcttac caaggttttg ctactggtca attggattat gatgccttcg tgccacgcca cctggtggat atggtgccaa acctgattgt tgcgcagagt ttctccaaga actttggcct gtacgggcat cgctgtggtg ctcttcacat ctcgacggcg agtgctgaag aggcgaagcg tttggtctca caattggccc tactgattcg gcccatgtat agcaatccgc ctctttatgg tgcctgggtc gtaagttcta ttctaaagga cccgcagctg actgcactct ggaagaagga attaaagcaa atgtcatctc gcattgctga ggtgcgcaag cgtttggttt cagagctgaa agcatgcggc tccgtccatg actggtctca cattgagcgc caagttggta tgatggcata cacaggccta acaagggagc aggtggagtt actgaggagc gagtaccaca tatacatgac gttgaatggt cgcgctgcgg tgtccggact taacagcaca aatgttgaat atgtttccca agctattcac aacgttacta aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for TrbrA.01471.a.B1.GE36914
HARVESTED ON: 5/8/2013
SEQUENCED ON: 5/8/2013
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGLNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAXRL VSQLALLIRP MYSNPPLYGA WVVSSILKDX XLXALWX
Validated NT Sequence
atggctcacc accaccacca ccatatgggg aaaccggatc ccatcctcgg cctcgggcag gattttcgta tggatcccgc caaaaggaag gtgaatttgt ctattggtgt gtaccgcgac gacgccgacc agccttttgt gctggaatgt gtgaaacaag ccacattagg caccaacatg gactacgccc cagtcacagg aattgcaagt tttgtagagg aggctcaaaa actgtgtttt ggcccaacgt gtgcggcgct tcgggatgga cgcattgcaa gttgccaaac gcttggcggc acaggtgcgc ttcggattgg tggtgacctt ctcaatcgct ttgtggccaa ttgtaaccgc atttacggtc ctgacgtcgg ttatccgaat catgagagta tctttgctaa ggcgggaatg gaacttaccc catattcgta ctatgatcct gccacaaaag gcttaaacct tgcgggtatg ctcgagtgcc tagacaaggc gcctgagggc tctgttatac tcgttcatgc ctgcgcacac aatccaactg gcgtcgatcc aacacacgat gactggcgac aggtgtgcga tgtgataaag aggcgtaacc atattccgtt cgtagacatg gcttaccaag gttttgctac tggtcaattg gattatgatg ccttcgtgcc acgccacctg gtggatatgg tgccaaacct gattgttgcg cagagtttct ccaagaactt tggcctgtac gggcatcgct gtggtgctct tcacatctca acggcgagtg ctgaagaggc gaancgtttg gtctcacaat tggccctact gattcggccc atgtatagca atccgcctct ttatggtgcc tgggtcgtaa gttctattct aaaggaccnn canctgantg cactctggna gaa
Expected Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIRP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGRAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcagg attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc agtcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tatccgaatc atgagagtat ctttgctaag gcggggatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg ttttaacctt gcgggtatgc tcgagtgcct agacaaggcg cctgagggct ctgttatact cgttcatgcc tgcgcacaca atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccaaacctg attgttgcgc agagtttctc caagaacttt ggcctgtacg ggcatcgctg tggtgctctt cacatctcga cggcgagtgc tgaagaggcg aagcgtttgg tctcacaatt ggccctactg attcggccca tgtatagcaa tccgcctctt tatggtgcct gggtcgtaag ttctattcta aaggacccgc agctgactgc actctggaag aaggaattaa agcaaatgtc atctcgcatt gctgaggtgc gcaagcgttt ggtttcagag ctgaaagcat gcggctccgt ccatgactgg tctcacattg agcgccaagt tggtatgatg gcatacacag gcctaacaag ggagcaggtg gagttactga ggagcgagta ccacatatac atgacgttga atggtcgcgc tgcggtgtcc ggacttaaca gcacaaatgt tgaatatgtt tcccaagcta ttcacaacgt tactaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for TrbrA.01471.a.B11.GE36562
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/8/2013
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG KPDPILGLGX DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPXTXIAS FVEEAQKLCF GPTCAALXDG RIASCQTLGG TXALRIGGDL LNRFVANCNR IYGPDVGWPN HESIFAKAGM ELTPYSYYDP ATKGLNLAXM LECLXXAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSXNFGLY GHRCGALHIS TASAEEAKRL VSQLXLLXXP MYSNPPLYGX WVVSSILKDX QXT
Validated NT Sequence
atggctcacc accaccacca ccatatgggg aaaccggatc ccatcctcgg cctcggnnnn gattttcgta tggatcccgc caaaaggaag gtgaatttgt ctattggtgt gtaccgcgac gacgccgacc agccttttgt gctggaatgt gtgaaacaag ccacattagg caccaacatg gactacgccc cantcacnng aattgcaagt tttgtagagg aggctcaaaa actgtgtttt ggcccaacgt gtgcggcgct tcnggatgga cgcattgcaa gttgccaaac gcttggcggc acangtgcgc ttcggattgg tggtgacctt ctcaatcgct ttgtggccaa ttgtaaccgc atttacggtc ctgacgtcgg ttggccgaat catgagagta tctttgctaa ggcgggaatg gaacttaccc catattcgta ctatgatcct gccacaaaag gcttaaacct tgcggnnatg ctcgagtgcc tnnnnnnngc gcctgagggc tctgttatac tcgttcatgc ctgcgcacac aatccaactg gcgtcgatcc aacacacgat gactggcgac aggtgtgcga tgtgataaag aggcgtaacc atattccgtt cgtagacatg gcttaccaag gttttgctac tggtcaattg gattatgatg ccttcgtgcc acgccacctg gtggatatgg tgccnaacct gattgttgcg cagagtttct ccnagaactt tggcctgtac gggcatcgct gtggtgctct tcacatctca acggcgagtg ctgaagaggc gaagcgtttg gtctcacaat tgnncctact gnntcngccc atgtatagca atccgcctct ttatggngnc tgggtcgtaa gttctattct aaaggaccnn cagntgactg c
Expected Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGWPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIRP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGRAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcagg attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc agtcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tggccgaatc atgagagtat ctttgctaag gcggggatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg ttttaacctt gcgggtatgc tcgagtgcct agacaaggcg cctgagggct ctgttatact cgttcatgcc tgcgcacaca atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccaaacctg attgttgcgc agagtttctc caagaacttt ggcctgtacg ggcatcgctg tggtgctctt cacatctcga cggcgagtgc tgaagaggcg aagcgtttgg tctcacaatt ggccctactg attcggccca tgtatagcaa tccgcctctt tatggtgcct gggtcgtaag ttctattcta aaggacccgc agctgactgc actctggaag aaggaattaa agcaaatgtc atctcgcatt gctgaggtgc gcaagcgttt ggtttcagag ctgaaagcat gcggctccgt ccatgactgg tctcacattg agcgccaagt tggtatgatg gcatacacag gcctaacaag ggagcaggtg gagttactga ggagcgagta ccacatatac atgacgttga atggtcgcgc tgcggtgtcc ggacttaaca gcacaaatgt tgaatatgtt tcccaagcta ttcacaacgt tactaaatga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrbrA.01471.a.B12.GE36563
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 78
Validated AA Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGLNLAGM LECLDKAPEG SVILVHACAH DPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIXP MYSNPPLYGA WVVSSILKDP QLTALWXXN
Validated NT Sequence
annnnnnttg tttaacttta agaaggagan ataccatggc tcaccaccac caccaccata tggggaaacc ggatcccatc ctcggcctcg ggcaggattt tcgtatggat cccgccaaaa ggaaggtgaa tttgtctatt ggtgtgtacc gcgacgacgc cgaccagcct tttgtgctgg aatgtgtgaa acaagccaca ttaggcacca acatggacta cgccccagtc acaggaattg caagttttgt agaggaggct caaaaactgt gttttggccc aacgtgtgcg gcgcttcggg atggacgcat tgcaagttgc caaacgcttg gcggcacagg tgcgcttcgg attggtggtg accttctcaa tcgctttgtg gccaattgta accgcattta cggtcctgac gtcggttatc cgaatcatga gagtatcttt gctaaggcgg gaatggaact taccccatat tcgtactatg atcctgccac aaaaggctta aaccttgcgg gtatgctcga gtgcctagac aaggcgcctg agggctctgt tatactcgtt catgcctgcg cacacgatcc aactggcgtc gatccaacac acgatgactg gcgacaggtg tgcgatgtga taaagaggcg taaccatatt ccgttcgtag acatggctta ccaaggtttt gctactggtc aattggatta tgatgccttc gtgccacgcc acctggtgga tatggtgcca aacctgattg ttgcgcagag tttctccaag aactttggcc tgtacgggca tcgctgtggt gctcttcaca tctcaacggc gagtgctgaa gaggcgaagc gtttggtctc acaattggcc ctactgattc ngcccatgta tagcaatccg cctctttatg gtgcctgggt cgtaagttct attctaaagg acccgcagct gactgcactc tggaananna attgaancaa atgtcatctc ncattgctga nngcgcnagc gtttggtttc nnagctnaag catgcggctn cntccatgac tggnctcncn ttgagcnnca agttggnntg anggcatann nnggnntaan nngnancnnn ngnanttact gnnnncgagt nnnnnntnnn cntgnnnnna ntggncnnnn ctnnnnngnc cngannnnnn nncnannntn aannttnnnn ngcnnntnnn anntnnnnnn nannnnnnan nannnga
Expected Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH DPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIRP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGRAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcagg attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc agtcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tatccgaatc atgagagtat ctttgctaag gcggggatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg ttttaacctt gcgggtatgc tcgagtgcct agacaaggcg cctgagggct ctgttatact cgttcatgcc tgcgcacacg atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccaaacctg attgttgcgc agagtttctc caagaacttt ggcctgtacg ggcatcgctg tggtgctctt cacatctcga cggcgagtgc tgaagaggcg aagcgtttgg tctcacaatt ggccctactg attcggccca tgtatagcaa tccgcctctt tatggtgcct gggtcgtaag ttctattcta aaggacccgc agctgactgc actctggaag aaggaattaa agcaaatgtc atctcgcatt gctgaggtgc gcaagcgttt ggtttcagag ctgaaagcat gcggctccgt ccatgactgg tctcacattg agcgccaagt tggtatgatg gcatacacag gcctaacaag ggagcaggtg gagttactga ggagcgagta ccacatatac atgacgttga atggtcgcgc tgcggtgtcc ggacttaaca gcacaaatgt tgaatatgtt tcccaagcta ttcacaacgt tactaaatga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrbrA.01471.a.B13.GE36564
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 95
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG KPDPILGLGX DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPXTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGLNLAGM LECLXXAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QXFSANFGLY GHRCGALXIS TASAEXAKRL VSXLALLIRX XYSNPPLYGA WXVSSILXDX S
Validated NT Sequence
tttgtttnnc tttaagaagg aganatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcang attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc antcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tatccgaatc atgagagtat ctttgctaag gcgggaatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg cttaaacctt gcgggtatgc tcgagtgcct nnnnnangcg cctgagggct ctgttatact cgttcatgcc tgcgcacaca atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccnaacctg attgttgcgc agantttctc cgcgaacttt ggcctgtacg ggcatcgctg tggtgctctt cncatctcaa cggcgagtgc tgaagangcg aagcgtttgg tctcacnatt ggccctactg attcggnccn tgtatagcaa tccgcctctt tatggtgcct gggncgtaag ttctattcta aangaccnca gctgactgca ntctgganaa ngaattgaag caaatgtcat ctcgcattgc tgagngcnca gcgnttggnt nnnnanctga aagcatgnnn tncntcatga ctggnnnnnc attgagnnnc anntggnnng anggcnnacn nngnnaacnn nnnnnngnng nnntactnnn nnnnnantac nnnnnnnnnn gacatnnaat ggnnnnntnc nnnncngnnn nnnnnnnnna annnnnnatn nnnnntntnn nnncntnnnn nnnnnnnnnn nnnnnnnnnn g
Expected Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSANFGLY GHRCGALHIS TASAEEAKRL VSQLALLIRP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGRAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcagg attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc agtcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tatccgaatc atgagagtat ctttgctaag gcggggatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg ttttaacctt gcgggtatgc tcgagtgcct agacaaggcg cctgagggct ctgttatact cgttcatgcc tgcgcacaca atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccaaacctg attgttgcgc agagtttctc cgcgaacttt ggcctgtacg ggcatcgctg tggtgctctt cacatctcga cggcgagtgc tgaagaggcg aagcgtttgg tctcacaatt ggccctactg attcggccca tgtatagcaa tccgcctctt tatggtgcct gggtcgtaag ttctattcta aaggacccgc agctgactgc actctggaag aaggaattaa agcaaatgtc atctcgcatt gctgaggtgc gcaagcgttt ggtttcagag ctgaaagcat gcggctccgt ccatgactgg tctcacattg agcgccaagt tggtatgatg gcatacacag gcctaacaag ggagcaggtg gagttactga ggagcgagta ccacatatac atgacgttga atggtcgcgc tgcggtgtcc ggacttaaca gcacaaatgt tgaatatgtt tcccaagcta ttcacaacgt tactaaatga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrbrA.01471.a.B15.GE36566
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 91
Validated AA Sequence
MDPAKRKVNL SIGVYRDDAD QPFVLECVKQ ATLGTNMDYA PVTGIASFVE EAQKLCFGPT CAALRDGRIA SCQTLGGTGA LRIGGDLLNR FVANCNRIYG PDVGYPNHES IFAKAGMELT PYSYYDPATK GLNLAGMLEC LDKAPEGSVI LVHACAHNPT GVDPTHDDWR QVCDVIKRRN HIPFVDMAYQ GFATGQLDYD AFVPRHLVDM VPNLIVAQSF SKNFGLYGHE CGALHISTAS AEEAKRLVSQ LALLIRPMYS NPPLYGAWVV SSILKDPQLT ALWKKELKQM SSRIAEVRKR LVSELKACGS VHDWSHIERQ VGMMAYTGLT REQVELLRSE YHIYMTLNGR AAVSGLNSTN VEYV
Validated NT Sequence
tgaaacatat tcaacatttg tgctgttaag tccggacacc gcagcgcgac catttaatgt catgtatatg tggtactcgc tcctcagtaa ctccacctgc tcccttgtta ggcctgtgta tgccatcata ccaacttggc gctcaatgtg agaccagtca tggacggagc cgcatgcttt cagctctgaa accaaacgct tgcgcacctc agcaatgcga gatgacattt gcttcaattc cttcttccag agtgcagtca gctgcgggtc ctttagaata gaacttacga cccaggcacc ataaagaggc ggattgctat acatgggccg aatcagtagg gccaattgtg agaccaaacg cttcgcctct tcagcactcg ccgttgagat gtgaagagca ccacactcat gcccgtacag gccaaagttc ttggagaaac tctgcgcaac aatcaggttt ggcaccatat ccaccaggtg gcgtggcacg aaggcatcat aatccaattg accagtagca aaaccttggt aagccatgtc tacgaacgga atatggttac gcctctttat cacatcgcac acctgtcgcc agtcatcgtg tgttggatcg acgccagttg gattgtgtgc gcaggcatga acgagtataa cagagccctc aggcgccttg tctaggcact cgagcatacc cgcaaggttt aagccttttg tggcaggatc atagtacgaa tatggggtaa gttccattcc cgccttagca aagatactct catgattcgg ataaccgacg tcaggaccgt aaatgcggtt acaattggcc acaaagcgat tgagaaggtc accaccaatc cgaagcgcac ctgtgccgcc aagcgtttgg caacttgcaa tgcgtccatc ccgaagcgcc gcacacgttg ggccaaaaca cagtttttga gcctcctcta caaaacttgc aattcctgtg actggggcgt agtccatgtt ggtgcctaat gtggcttgtt tcacacattc cagcacaaaa ggctggtcgg cgtcgtcgcg gtacacacca atagacaaat tcaccttcct tttggcggga tccatacgaa aatcctgccc gaggccgagg atgggatccg gttt
Expected Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHECGALHIS TASAEEAKRL VSQLALLIRP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGRAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcagg attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc agtcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tatccgaatc atgagagtat ctttgctaag gcggggatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg ttttaacctt gcgggtatgc tcgagtgcct agacaaggcg cctgagggct ctgttatact cgttcatgcc tgcgcacaca atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccaaacctg attgttgcgc agagtttctc caagaacttt ggcctgtacg ggcatgagtg tggtgctctt cacatctcga cggcgagtgc tgaagaggcg aagcgtttgg tctcacaatt ggccctactg attcggccca tgtatagcaa tccgcctctt tatggtgcct gggtcgtaag ttctattcta aaggacccgc agctgactgc actctggaag aaggaattaa agcaaatgtc atctcgcatt gctgaggtgc gcaagcgttt ggtttcagag ctgaaagcat gcggctccgt ccatgactgg tctcacattg agcgccaagt tggtatgatg gcatacacag gcctaacaag ggagcaggtg gagttactga ggagcgagta ccacatatac atgacgttga atggtcgcgc tgcggtgtcc ggacttaaca gcacaaatgt tgaatatgtt tcccaagcta ttcacaacgt tactaaatga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrbrA.01471.a.B16.GE36567
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGLNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSXNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIEP MYSNPPLYGA WVVSSILXDX QXTALWXXEX XQMSSPLLXA QAFXXXXESM XXXXDWXHIX XXXXXWXTXX XGXGXXTXXX XXXXXXXXXX XXXXXXXXXX XXXXSXXXXX XXX
Validated NT Sequence
annnntttgt ttaactttaa gaaggagana taccatggct caccaccacc accaccatat ggggaaaccg gatcccatcc tcggcctcgg gcaggatttt cgtatggatc ccgccaaaag gaaggtgaat ttgtctattg gtgtgtaccg cgacgacgcc gaccagcctt ttgtgctgga atgtgtgaaa caagccacat taggcaccaa catggactac gccccagtca caggaattgc aagttttgta gaggaggctc aaaaactgtg ttttggccca acgtgtgcgg cgcttcggga tggacgcatt gcaagttgcc aaacgcttgg cggcacaggt gcgcttcgga ttggtggtga ccttctcaat cgctttgtgg ccaattgtaa ccgcatttac ggtcctgacg tcggttatcc gaatcatgag agtatctttg ctaaggcggg aatggaactt accccatatt cgtactatga tcctgccaca aaaggcttaa accttgcggg tatgctcgag tgcctagaca aggcgcctga gggctctgtt atactcgttc atgcctgcgc acacaatcca actggcgtcg atccaacaca cgatgactgg cgacaggtgt gcgatgtgat aaagaggcgt aaccatattc cgttcgtaga catggcttac caaggttttg ctactggtca attggattat gatgccttcg tgccacgcca cctggtggat atggtgccaa acctgattgt tgcgcagagt ttctccnaga actttggcct gtacgggcat cgctgtggtg ctcttcacat ctcaacggcg agtgctgaag aggcgaagcg tttggtctca caattggccc tactgattga gcccatgtat agcaatccgc ctctttatgg tgcctgggtc gtaagttcta ttctaaanga ccnncagntg actgcnctct ggaanaanga antgnancaa atgtcatctc cattgctgnn ngcncaagcg tttngnttnn nanntgaaag catgnnnnnc nnncntgact ggnntcacat tgnncnnnag tnggnannnt ggnntacncn nnnacnnggn ncnggngnnn ttactgnnna ncnnnnncnn nnnnnnntga nntnaanngn nnncntnnnn nnnnnnnnnn nnnnnnnnnn annnnnttnn nncnnntcnn nnnnnnnnna annnnnnnnn nana
Expected Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIEP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGRAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcagg attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc agtcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tatccgaatc atgagagtat ctttgctaag gcggggatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg ttttaacctt gcgggtatgc tcgagtgcct agacaaggcg cctgagggct ctgttatact cgttcatgcc tgcgcacaca atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccaaacctg attgttgcgc agagtttctc caagaacttt ggcctgtacg ggcatcgctg tggtgctctt cacatctcga cggcgagtgc tgaagaggcg aagcgtttgg tctcacaatt ggccctactg attgagccca tgtatagcaa tccgcctctt tatggtgcct gggtcgtaag ttctattcta aaggacccgc agctgactgc actctggaag aaggaattaa agcaaatgtc atctcgcatt gctgaggtgc gcaagcgttt ggtttcagag ctgaaagcat gcggctccgt ccatgactgg tctcacattg agcgccaagt tggtatgatg gcatacacag gcctaacaag ggagcaggtg gagttactga ggagcgagta ccacatatac atgacgttga atggtcgcgc tgcggtgtcc ggacttaaca gcacaaatgt tgaatatgtt tcccaagcta ttcacaacgt tactaaatga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrbrA.01471.a.B17.GE36568
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 95
PERCENT COVERAGE: 82
Validated AA Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGLNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSXNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIXP MYSNPPLYGA WVVSSILKDX QLTALWXXXL KXMSSRIAXX ASVXXXX
Validated NT Sequence
tttgtttaac tttaagaagg aganatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcagg attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc agtcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tatccgaatc atgagagtat ctttgctaag gcgggaatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg cttaaacctt gcgggtatgc tcgagtgcct agacaaggcg cctgagggct ctgttatact cgttcatgcc tgcgcacaca atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccaaacctg attgttgcgc agagtttctc cnagaacttt ggcctgtacg ggcatcgctg tggtgctctt cacatctcaa cggcgagtgc tgaagaggcg aagcgtttgg tctcacaatt ggccctactg attcngccca tgtatagcaa tccgcctctt tatggtgcct gggtcgtaag ttctattcta aaggaccngc agctgactgc nctctggaan nannaattga agcnaatgtc atctcgcatt gctganngcg cnagcgtttn gnttnnnanc tgaaagcatg cngctccnnc ntgactggnn tcacnttnnn nnnnnnngna ngnngnntan nnngnannnn gnnncngnng nntnctnnnn annnannacn nnatnnnnnt gactnannnn nngnnnnnnn nnnnnnnnnn nnnnnaannn nnannnnnnt nnnnnnntnn na
Expected Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIRP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGEAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcagg attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc agtcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tatccgaatc atgagagtat ctttgctaag gcggggatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg ttttaacctt gcgggtatgc tcgagtgcct agacaaggcg cctgagggct ctgttatact cgttcatgcc tgcgcacaca atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccaaacctg attgttgcgc agagtttctc caagaacttt ggcctgtacg ggcatcgctg tggtgctctt cacatctcga cggcgagtgc tgaagaggcg aagcgtttgg tctcacaatt ggccctactg attcggccca tgtatagcaa tccgcctctt tatggtgcct gggtcgtaag ttctattcta aaggacccgc agctgactgc actctggaag aaggaattaa agcaaatgtc atctcgcatt gctgaggtgc gcaagcgttt ggtttcagag ctgaaagcat gcggctccgt ccatgactgg tctcacattg agcgccaagt tggtatgatg gcatacacag gcctaacaag ggagcaggtg gagttactga ggagcgagta ccacatatac atgacgttga atggtgaggc tgcggtgtcc ggacttaaca gcacaaatgt tgaatatgtt tcccaagcta ttcacaacgt tactaaatga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrbrA.01471.a.B11.PS01759
PURIFICATION DATe: 2/27/2013
CONCENTRATION: 29mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 94
PERCENT COVERAGE: 76
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG KPDPILGLGX DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPXTXIAS FVEEAQKLCF GPTCAALXDG RIASCQTLGG TXALRIGGDL LNRFVANCNR IYGPDVGWPN HESIFAKAGM ELTPYSYYDP ATKGLNLAXM LECLXXAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSXNFGLY GHRCGALHIS TASAEEAKRL VSQLXLLXXP MYSNPPLYGX WVVSSILKDX QXT
Validated NT Sequence
atggctcacc accaccacca ccatatgggg aaaccggatc ccatcctcgg cctcggnnnn gattttcgta tggatcccgc caaaaggaag gtgaatttgt ctattggtgt gtaccgcgac gacgccgacc agccttttgt gctggaatgt gtgaaacaag ccacattagg caccaacatg gactacgccc cantcacnng aattgcaagt tttgtagagg aggctcaaaa actgtgtttt ggcccaacgt gtgcggcgct tcnggatgga cgcattgcaa gttgccaaac gcttggcggc acangtgcgc ttcggattgg tggtgacctt ctcaatcgct ttgtggccaa ttgtaaccgc atttacggtc ctgacgtcgg ttggccgaat catgagagta tctttgctaa ggcgggaatg gaacttaccc catattcgta ctatgatcct gccacaaaag gcttaaacct tgcggnnatg ctcgagtgcc tnnnnnnngc gcctgagggc tctgttatac tcgttcatgc ctgcgcacac aatccaactg gcgtcgatcc aacacacgat gactggcgac aggtgtgcga tgtgataaag aggcgtaacc atattccgtt cgtagacatg gcttaccaag gttttgctac tggtcaattg gattatgatg ccttcgtgcc acgccacctg gtggatatgg tgccnaacct gattgttgcg cagagtttct ccnagaactt tggcctgtac gggcatcgct gtggtgctct tcacatctca acggcgagtg ctgaagaggc gaagcgtttg gtctcacaat tgnncctact gnntcngccc atgtatagca atccgcctct ttatggngnc tgggtcgtaa gttctattct aaaggaccnn cagntgactg c
Expressed Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGWPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIRP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGRAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGGA AACCGGATCC CATCCTCGGC CTCGGGCAGG ATTTTCGTAT GGATCCCGCC AAAAGGAAGG TGAATTTGTC TATTGGTGTG TACCGCGACG ACGCCGACCA GCCTTTTGTG CTGGAATGTG TGAAACAAGC CACATTAGGC ACCAACATGG ACTACGCCCC AGTCACAGGA ATTGCAAGTT TTGTAGAGGA GGCTCAAAAA CTGTGTTTTG GCCCAACGTG TGCGGCGCTT CGGGATGGAC GCATTGCAAG TTGCCAAACG CTTGGCGGCA CAGGTGCGCT TCGGATTGGT GGTGACCTTC TCAATCGCTT TGTGGCCAAT TGTAACCGCA TTTACGGTCC TGACGTCGGT TGGCCGAATC ATGAGAGTAT CTTTGCTAAG GCGGGGATGG AACTTACCCC ATATTCGTAC TATGATCCTG CCACAAAAGG TTTTAACCTT GCGGGTATGC TCGAGTGCCT AGACAAGGCG CCTGAGGGCT CTGTTATACT CGTTCATGCC TGCGCACACA ATCCAACTGG CGTCGATCCA ACACACGATG ACTGGCGACA GGTGTGCGAT GTGATAAAGA GGCGTAACCA TATTCCGTTC GTAGACATGG CTTACCAAGG TTTTGCTACT GGTCAATTGG ATTATGATGC CTTCGTGCCA CGCCACCTGG TGGATATGGT GCCAAACCTG ATTGTTGCGC AGAGTTTCTC CAAGAACTTT GGCCTGTACG GGCATCGCTG TGGTGCTCTT CACATCTCGA CGGCGAGTGC TGAAGAGGCG AAGCGTTTGG TCTCACAATT GGCCCTACTG ATTCGGCCCA TGTATAGCAA TCCGCCTCTT TATGGTGCCT GGGTCGTAAG TTCTATTCTA AAGGACCCGC AGCTGACTGC ACTCTGGAAG AAGGAATTAA AGCAAATGTC ATCTCGCATT GCTGAGGTGC GCAAGCGTTT GGTTTCAGAG CTGAAAGCAT GCGGCTCCGT CCATGACTGG TCTCACATTG AGCGCCAAGT TGGTATGATG GCATACACAG GCCTAACAAG GGAGCAGGTG GAGTTACTGA GGAGCGAGTA CCACATATAC ATGACGTTGA ATGGTCGCGC TGCGGTGTCC GGACTTAACA GCACAAATGT TGAATATGTT TCCCAAGCTA TTCACAACGT TACTAAATGA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for TrbrA.01471.a.B13.PS01760
PURIFICATION DATe: 2/27/2013
CONCENTRATION: 28.8mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 95
PERCENT COVERAGE: 76
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG KPDPILGLGX DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPXTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGLNLAGM LECLXXAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QXFSANFGLY GHRCGALXIS TASAEXAKRL VSXLALLIRX XYSNPPLYGA WXVSSILXDX S
Validated NT Sequence
tttgtttnnc tttaagaagg aganatacca tggctcacca ccaccaccac catatgggga aaccggatcc catcctcggc ctcgggcang attttcgtat ggatcccgcc aaaaggaagg tgaatttgtc tattggtgtg taccgcgacg acgccgacca gccttttgtg ctggaatgtg tgaaacaagc cacattaggc accaacatgg actacgcccc antcacagga attgcaagtt ttgtagagga ggctcaaaaa ctgtgttttg gcccaacgtg tgcggcgctt cgggatggac gcattgcaag ttgccaaacg cttggcggca caggtgcgct tcggattggt ggtgaccttc tcaatcgctt tgtggccaat tgtaaccgca tttacggtcc tgacgtcggt tatccgaatc atgagagtat ctttgctaag gcgggaatgg aacttacccc atattcgtac tatgatcctg ccacaaaagg cttaaacctt gcgggtatgc tcgagtgcct nnnnnangcg cctgagggct ctgttatact cgttcatgcc tgcgcacaca atccaactgg cgtcgatcca acacacgatg actggcgaca ggtgtgcgat gtgataaaga ggcgtaacca tattccgttc gtagacatgg cttaccaagg ttttgctact ggtcaattgg attatgatgc cttcgtgcca cgccacctgg tggatatggt gccnaacctg attgttgcgc agantttctc cgcgaacttt ggcctgtacg ggcatcgctg tggtgctctt cncatctcaa cggcgagtgc tgaagangcg aagcgtttgg tctcacnatt ggccctactg attcggnccn tgtatagcaa tccgcctctt tatggtgcct gggncgtaag ttctattcta aangaccnca gctgactgca ntctgganaa ngaattgaag caaatgtcat ctcgcattgc tgagngcnca gcgnttggnt nnnnanctga aagcatgnnn tncntcatga ctggnnnnnc attgagnnnc anntggnnng anggcnnacn nngnnaacnn nnnnnngnng nnntactnnn nnnnnantac nnnnnnnnnn gacatnnaat ggnnnnntnc nnnncngnnn nnnnnnnnna annnnnnatn nnnnntntnn nnncntnnnn nnnnnnnnnn nnnnnnnnnn g
Expressed Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSANFGLY GHRCGALHIS TASAEEAKRL VSQLALLIRP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGRAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGGA AACCGGATCC CATCCTCGGC CTCGGGCAGG ATTTTCGTAT GGATCCCGCC AAAAGGAAGG TGAATTTGTC TATTGGTGTG TACCGCGACG ACGCCGACCA GCCTTTTGTG CTGGAATGTG TGAAACAAGC CACATTAGGC ACCAACATGG ACTACGCCCC AGTCACAGGA ATTGCAAGTT TTGTAGAGGA GGCTCAAAAA CTGTGTTTTG GCCCAACGTG TGCGGCGCTT CGGGATGGAC GCATTGCAAG TTGCCAAACG CTTGGCGGCA CAGGTGCGCT TCGGATTGGT GGTGACCTTC TCAATCGCTT TGTGGCCAAT TGTAACCGCA TTTACGGTCC TGACGTCGGT TATCCGAATC ATGAGAGTAT CTTTGCTAAG GCGGGGATGG AACTTACCCC ATATTCGTAC TATGATCCTG CCACAAAAGG TTTTAACCTT GCGGGTATGC TCGAGTGCCT AGACAAGGCG CCTGAGGGCT CTGTTATACT CGTTCATGCC TGCGCACACA ATCCAACTGG CGTCGATCCA ACACACGATG ACTGGCGACA GGTGTGCGAT GTGATAAAGA GGCGTAACCA TATTCCGTTC GTAGACATGG CTTACCAAGG TTTTGCTACT GGTCAATTGG ATTATGATGC CTTCGTGCCA CGCCACCTGG TGGATATGGT GCCAAACCTG ATTGTTGCGC AGAGTTTCTC CGCGAACTTT GGCCTGTACG GGCATCGCTG TGGTGCTCTT CACATCTCGA CGGCGAGTGC TGAAGAGGCG AAGCGTTTGG TCTCACAATT GGCCCTACTG ATTCGGCCCA TGTATAGCAA TCCGCCTCTT TATGGTGCCT GGGTCGTAAG TTCTATTCTA AAGGACCCGC AGCTGACTGC ACTCTGGAAG AAGGAATTAA AGCAAATGTC ATCTCGCATT GCTGAGGTGC GCAAGCGTTT GGTTTCAGAG CTGAAAGCAT GCGGCTCCGT CCATGACTGG TCTCACATTG AGCGCCAAGT TGGTATGATG GCATACACAG GCCTAACAAG GGAGCAGGTG GAGTTACTGA GGAGCGAGTA CCACATATAC ATGACGTTGA ATGGTCGCGC TGCGGTGTCC GGACTTAACA GCACAAATGT TGAATATGTT TCCCAAGCTA TTCACAACGT TACTAAATGA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for TrbrA.01471.a.A1.PW34891
PURIFICATION DATe: 2/17/2012
CONCENTRATION: 27.7mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 62
Protocol Notes
notes unavailable
Validated AA Sequence
MGKPDPILGL GQDFRMDPAK RKVNLSIGVY RDDADQPFVL ECVKQATLGT NMDYAPVTGI ASFVEEAQKL CFGPTCAALR DGRIASCQTL GGTGALRIGG DLLNRFVANC NRIYGPDVGY PNHESIFAKA GMELTPYSYY DPATKGLNLA GMLECLDKAP EGSVILVHAC AHNPTGVDPT HDDWRQVCDV IKRRNHIPFV DMAYQGFATG QLDYDAFVPR HLVDMVPNLI VAQSFSKNFX PVRASLWCSS HLNGEC
Validated NT Sequence
gtaacgttgt gaatagcttg tgaaacatat tcaacatttg tgctgttaag tccggacacc gcagcgcgac catttaatgt catgtatatg tggtactcgc tcctcagtaa ctccacctgc tcccttgtta ggcctgtgta tgccatcata ccaacttggc gctcaatgtg agaccagtca tggacggagc cgcatgcttt cagctctgaa accaaacgct tgcgcacctc agcaatgcga gatgacattt gcttcaattc cttcttccag agtgcagtca gctgcgggtc ctttagaata gaacttacga cccaggcacc ataaagaggc ggattgttat acatgggccg aatcagtagg gccaattgtg agaccaaacg cttcgcctct tcagcactcg ccgttgagat gtgaagagca ccacagcgat gcccgtacag gccnaaagtt cttggagaaa ctctgcgcaa caatcaggtt tggcaccata tccaccaggt ggcgtggcac gaaggcatca taatccaatt gaccagtagc aaaaccttgg taagccatgt ctacgaacgg aatatggtta cgcctcttta tcacatcgca cacctgtcgc cagtcatcgt gtgttggatc gacgccagtt ggattgtgtg cgcaggcatg aacgagtata acagagccct caggcgcctt gtctaggcac tcgagcatac ccgcaaggtt taagcctttt gtggcaggat catagtacga atatggggta agttccattc ccgccttagc aaagatactc tcatgattcg gataaccgac gtcaggaccg taaatgcggt tacaattggc cacaaagcga ttgagaaggt caccaccaat ccgaagcgca cctgtgccgc caagcgtttg gcaacttgca atgcgtccat cccgaagcgc cgcacacgtt gggccaaaac acagtttttg agcctcctct acaaaacttg caattcctgt gactggggcg tagtccatgt tggtgcctaa tgtggcttgt ttcacacatt ccagcacaaa aggctggtcg gcgtcgtcgc ggtacacacc aatagacaaa ttcaccttcc ttttggcggg atccatacga aaatcctgcc cgaggccgag gatgggatcc ggtttcccca tcgaaccagg accctgggtc tgagcttcca gggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGKPDPILG LGQDFRMDPA KRKVNLSIGV YRDDADQPFV LECVKQATLG TNMDYAPVTG IASFVEEAQK LCFGPTCAAL RDGRIASCQT LGGTGALRIG GDLLNRFVAN CNRIYGPDVG YPNHESIFAK AGMELTPYSY YDPATKGFNL AGMLECLDKA PEGSVILVHA CAHNPTGVDP THDDWRQVCD VIKRRNHIPF VDMAYQGFAT GQLDYDAFVP RHLVDMVPNL IVAQSFSKNF GLYGHRCGAL HISTASAEEA KRLVSQLALL IRPMYSNPPL YGAWVVSSIL KDPQLTALWK KELKQMSSRI AEVRKRLVSE LKACGSVHDW SHIERQVGMM AYTGLTREQV ELLRSEYHIY MTLNGRAAVS GLNSTNVEYV SQAIHNVTK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGGAAACCG GATCCCATCC TCGGCCTCGG GCAGGATTTT CGTATGGATC CCGCCAAAAG GAAGGTGAAT TTGTCTATTG GTGTGTACCG CGACGACGCC GACCAGCCTT TTGTGCTGGA ATGTGTGAAA CAAGCCACAT TAGGCACCAA CATGGACTAC GCCCCAGTCA CAGGAATTGC AAGTTTTGTA GAGGAGGCTC AAAAACTGTG TTTTGGCCCA ACGTGTGCGG CGCTTCGGGA TGGACGCATT GCAAGTTGCC AAACGCTTGG CGGCACAGGT GCGCTTCGGA TTGGTGGTGA CCTTCTCAAT CGCTTTGTGG CCAATTGTAA CCGCATTTAC GGTCCTGACG TCGGTTATCC GAATCATGAG AGTATCTTTG CTAAGGCGGG GATGGAACTT ACCCCATATT CGTACTATGA TCCTGCCACA AAAGGTTTTA ACCTTGCGGG TATGCTCGAG TGCCTAGACA AGGCGCCTGA GGGCTCTGTT ATACTCGTTC ATGCCTGCGC ACACAATCCA ACTGGCGTCG ATCCAACACA CGATGACTGG CGACAGGTGT GCGATGTGAT AAAGAGGCGT AACCATATTC CGTTCGTAGA CATGGCTTAC CAAGGTTTTG CTACTGGTCA ATTGGATTAT GATGCCTTCG TGCCACGCCA CCTGGTGGAT ATGGTGCCAA ACCTGATTGT TGCGCAGAGT TTCTCCAAGA ACTTTGGCCT GTACGGGCAT CGCTGTGGTG CTCTTCACAT CTCGACGGCG AGTGCTGAAG AGGCGAAGCG TTTGGTCTCA CAATTGGCCC TACTGATTCG GCCCATGTAT AGCAATCCGC CTCTTTATGG TGCCTGGGTC GTAAGTTCTA TTCTAAAGGA CCCGCAGCTG ACTGCACTCT GGAAGAAGGA ATTAAAGCAA ATGTCATCTC GCATTGCTGA GGTGCGCAAG CGTTTGGTTT CAGAGCTGAA AGCATGCGGC TCCGTCCATG ACTGGTCTCA CATTGAGCGC CAAGTTGGTA TGATGGCATA CACAGGCCTA ACAAGGGAGC AGGTGGAGTT ACTGAGGAGC GAGTACCACA TATACATGAC GTTGAATGGT CGCGCTGCGG TGTCCGGACT TAACAGCACA AATGTTGAAT ATGTTTCCCA AGCTATTCAC AACGTTACTA AATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for TrbrA.01471.a.B1.PW37325
PURIFICATION DATe: 7/8/2013
CONCENTRATION: 20mg/ml
OBSERVED MW: 43kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 77
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGLNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAXRL VSQLALLIRP MYSNPPLYGA WVVSSILKDX XLXALWX
Validated NT Sequence
atggctcacc accaccacca ccatatgggg aaaccggatc ccatcctcgg cctcgggcag gattttcgta tggatcccgc caaaaggaag gtgaatttgt ctattggtgt gtaccgcgac gacgccgacc agccttttgt gctggaatgt gtgaaacaag ccacattagg caccaacatg gactacgccc cagtcacagg aattgcaagt tttgtagagg aggctcaaaa actgtgtttt ggcccaacgt gtgcggcgct tcgggatgga cgcattgcaa gttgccaaac gcttggcggc acaggtgcgc ttcggattgg tggtgacctt ctcaatcgct ttgtggccaa ttgtaaccgc atttacggtc ctgacgtcgg ttatccgaat catgagagta tctttgctaa ggcgggaatg gaacttaccc catattcgta ctatgatcct gccacaaaag gcttaaacct tgcgggtatg ctcgagtgcc tagacaaggc gcctgagggc tctgttatac tcgttcatgc ctgcgcacac aatccaactg gcgtcgatcc aacacacgat gactggcgac aggtgtgcga tgtgataaag aggcgtaacc atattccgtt cgtagacatg gcttaccaag gttttgctac tggtcaattg gattatgatg ccttcgtgcc acgccacctg gtggatatgg tgccaaacct gattgttgcg cagagtttct ccaagaactt tggcctgtac gggcatcgct gtggtgctct tcacatctca acggcgagtg ctgaagaggc gaancgtttg gtctcacaat tggccctact gattcggccc atgtatagca atccgcctct ttatggtgcc tgggtcgtaa gttctattct aaaggaccnn canctgantg cactctggna gaa
Expressed Protein Sequence
MAHHHHHHMG KPDPILGLGQ DFRMDPAKRK VNLSIGVYRD DADQPFVLEC VKQATLGTNM DYAPVTGIAS FVEEAQKLCF GPTCAALRDG RIASCQTLGG TGALRIGGDL LNRFVANCNR IYGPDVGYPN HESIFAKAGM ELTPYSYYDP ATKGFNLAGM LECLDKAPEG SVILVHACAH NPTGVDPTHD DWRQVCDVIK RRNHIPFVDM AYQGFATGQL DYDAFVPRHL VDMVPNLIVA QSFSKNFGLY GHRCGALHIS TASAEEAKRL VSQLALLIRP MYSNPPLYGA WVVSSILKDP QLTALWKKEL KQMSSRIAEV RKRLVSELKA CGSVHDWSHI ERQVGMMAYT GLTREQVELL RSEYHIYMTL NGRAAVSGLN STNVEYVSQA IHNVTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGGA AACCGGATCC CATCCTCGGC CTCGGGCAGG ATTTTCGTAT GGATCCCGCC AAAAGGAAGG TGAATTTGTC TATTGGTGTG TACCGCGACG ACGCCGACCA GCCTTTTGTG CTGGAATGTG TGAAACAAGC CACATTAGGC ACCAACATGG ACTACGCCCC AGTCACAGGA ATTGCAAGTT TTGTAGAGGA GGCTCAAAAA CTGTGTTTTG GCCCAACGTG TGCGGCGCTT CGGGATGGAC GCATTGCAAG TTGCCAAACG CTTGGCGGCA CAGGTGCGCT TCGGATTGGT GGTGACCTTC TCAATCGCTT TGTGGCCAAT TGTAACCGCA TTTACGGTCC TGACGTCGGT TATCCGAATC ATGAGAGTAT CTTTGCTAAG GCGGGGATGG AACTTACCCC ATATTCGTAC TATGATCCTG CCACAAAAGG TTTTAACCTT GCGGGTATGC TCGAGTGCCT AGACAAGGCG CCTGAGGGCT CTGTTATACT CGTTCATGCC TGCGCACACA ATCCAACTGG CGTCGATCCA ACACACGATG ACTGGCGACA GGTGTGCGAT GTGATAAAGA GGCGTAACCA TATTCCGTTC GTAGACATGG CTTACCAAGG TTTTGCTACT GGTCAATTGG ATTATGATGC CTTCGTGCCA CGCCACCTGG TGGATATGGT GCCAAACCTG ATTGTTGCGC AGAGTTTCTC CAAGAACTTT GGCCTGTACG GGCATCGCTG TGGTGCTCTT CACATCTCGA CGGCGAGTGC TGAAGAGGCG AAGCGTTTGG TCTCACAATT GGCCCTACTG ATTCGGCCCA TGTATAGCAA TCCGCCTCTT TATGGTGCCT GGGTCGTAAG TTCTATTCTA AAGGACCCGC AGCTGACTGC ACTCTGGAAG AAGGAATTAA AGCAAATGTC ATCTCGCATT GCTGAGGTGC GCAAGCGTTT GGTTTCAGAG CTGAAAGCAT GCGGCTCCGT CCATGACTGG TCTCACATTG AGCGCCAAGT TGGTATGATG GCATACACAG GCCTAACAAG GGAGCAGGTG GAGTTACTGA GGAGCGAGTA CCACATATAC ATGACGTTGA ATGGTCGCGC TGCGGTGTCC GGACTTAACA GCACAAATGT TGAATATGTT TCCCAAGCTA TTCACAACGT TACTAAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT