TrbrA.01427.a

inorganic pyrophosphatase, putative

CENTER ID: TrbrA.01427.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.01427.a.A2.GE34289 mature protein 28 261
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.3.2840
RefSeq: XP_843877.1
UniProt: Q57ZM8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPTSIMFKG MTGAGIMLPA WALQEVGAAG TRAWRMYFTS SEAGSVARRS AWHDLPLHPS PDASVITFVC EIPRRTRAKL ELVKEEPHNP IAQDTLKKEG NALRFFKYGD VPFNYGFAPQ TWEDPSVMDQ LTTCGGDGDP IDIVELSSNP FAVGSVRAVR VLGLLGLIDE GETDWKVITE AIGPDATGTY GSLNNVPQEL KATIVKWFRE YKTADGKKPN EFVFGGELRN ADDALRVIEG GSRQYTGLIA GTVRNPGYWL H
NT Sequence
atgcgcccaa catcaattat gtttaaaggg atgaccggag caggcattat gctccccgca tgggcattgc aggaagtggg agcagcggga acgcgggcgt ggcgcatgta cttcacaagc agcgaggccg gatccgttgc gaggcgctcg gcatggcatg atcttccgct acatccatca cctgatgcat ctgtcattac atttgtatgt gagattccca gaaggacgcg tgccaaattg gaactcgtga aggaagaacc gcacaacccg atagctcaag acacattaaa gaaagagggc aatgctttgc ggttcttcaa gtatggtgat gtccccttca actatggttt tgcgccacaa acatgggagg atccttcagt aatggaccaa ctgacgacgt gtgggggtga tggtgatccc attgatatcg ttgaattatc gtcaaatccc tttgcagttg gttccgtaag ggcagttcga gtcctcgggt tgttgggact tattgacgag ggggaaaccg actggaaagt cattacggag gccattggac ccgatgcgac cggtacgtac gggtctttaa ataatgtgcc acaagagctg aaggctacca ttgttaaatg gttccgcgag tacaaaactg ctgatgggaa gaagccaaat gaatttgttt ttggagggga gttgcgtaat gcagacgacg cacttcgtgt gattgaagga ggttcccgcc agtacaccgg acttattgca ggcacggttc gtaatcccgg ttactggctt cactga
Details for TrbrA.01427.a.A2.GE34289
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAGTRAWR MYFTSSEAGS VARRSAWHDL PLHPSPDASV ITFVCEIPRR TRAKLELVKE EPHNPIAQDT LKKEGNALRF FKYGDVPFNY GFAPQTWEDP SVVDQLTTCG GDGDPIDIVE LSSNPFAVGS VRAVRVLGLL GLIDEGETDW KVITEAIGPD ATGTYGSLNN VPQELKATIV KWFREYKTAD GKKPNEFVFG GELRNADDAL RVIEGGSRQY TGLIAGTVRN PGYWLH
Validated NT Sequence
attttntnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcag cgggaacgcg ggcgtggcgc atgtacttca caagcagcga ggccggatcc gttgcgaggc gctcggcatg gcatgatctt ccgctacatc catcacctga tgcatctgtc attacatttg tatgtgagat tcccagaagg acgcgtgcca aattggaact cgtgaaggaa gaaccgcaca acccgatagc tcaagacaca ttaaagaaag agggcaatgc tttgcggttc ttcaagtatg gtgatgtccc cttcaactat ggttttgcgc cacaaacatg ggaggatcct tcagtagtgg accaactgac gacgtgtggg ggtgatggtg atcccattga tatcgttgaa ttatcgtcaa atccctttgc agttggttcc gtaagggcag ttcgagtcct cgggttgtta ggacttattg acgaggggga aaccgactgg aaagtcatta cggaggccat tggacccgat gcgaccggta cgtacgggtc tttaaataat gtgccacaag agctgaaggc taccattgtt aaatggttcc gcgagtacaa aactgctgat gggaagaagc caaatgaatt tgtttttgga ggggagttgc gtaatgcaga cgacgcactt cgtgtgattg aaggaggttc ccgccagtac accggactta ttgcaggcac ggttcgtaat cccggttact ggcttcacta ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctganttgg ctgctgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAGTRAWR MYFTSSEAGS VARRSAWHDL PLHPSPDASV ITFVCEIPRR TRAKLELVKE EPHNPIAQDT LKKEGNALRF FKYGDVPFNY GFAPQTWEDP SVMDQLTTCG GDGDPIDIVE LSSNPFAVGS VRAVRVLGLL GLIDEGETDW KVITEAIGPD ATGTYGSLNN VPQELKATIV KWFREYKTAD GKKPNEFVFG GELRNADDAL RVIEGGSRQY TGLIAGTVRN PGYWLH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagcggga acgcgggcgt ggcgcatgta cttcacaagc agcgaggccg gatccgttgc gaggcgctcg gcatggcatg atcttccgct acatccatca cctgatgcat ctgtcattac atttgtatgt gagattccca gaaggacgcg tgccaaattg gaactcgtga aggaagaacc gcacaacccg atagctcaag acacattaaa gaaagagggc aatgctttgc ggttcttcaa gtatggtgat gtccccttca actatggttt tgcgccacaa acatgggagg atccttcagt aatggaccaa ctgacgacgt gtgggggtga tggtgatccc attgatatcg ttgaattatc gtcaaatccc tttgcagttg gttccgtaag ggcagttcga gtcctcgggt tgttgggact tattgacgag ggggaaaccg actggaaagt cattacggag gccattggac ccgatgcgac cggtacgtac gggtctttaa ataatgtgcc acaagagctg aaggctacca ttgttaaatg gttccgcgag tacaaaactg ctgatgggaa gaagccaaat gaatttgttt ttggagggga gttgcgtaat gcagacgacg cacttcgtgt gattgaagga ggttcccgcc agtacaccgg acttattgca ggcacggttc gtaatcccgg ttactggctt cactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa