TrbrA.01405.a
adenine phosphoribosyltransferase
| CENTER ID: | TrbrA.01405.a |
| ORGANISM: | Trypanosoma brucei TREU927 |
| ASSOCIATED DISEASE: | African trypanosomiasis (African Sleeping Sickness) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrbrA.01405.a.B1.GE40765 | full length | 1 | 235 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrbrA.01405.a.B1.PS38186 | full length | 1 | 235 |
Structures
5VN4
DEPOSITED: 4/28/2017
DETERMINATION: XRay
CLONE: TrbrA.01405.a.B1.GE40765
PROTEIN: TrbrA.01405.a.B1.PS38186
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:Tb927.7.1780 |
| OrthoMCL: | OG5_127379 |
| RefSeq: | XP_845802.1 |
| UniProt: | Q57V32 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLVEVLPNY FTLSKDSPLR KKFEKVYKWY SPAFSPHDVP RFAEVGNITE NPEVMRGIRD FFVDRYKNLQ QPITHILGFD SRGFLLGPMI AVELNVPFVL IRKANKIAGV IIKSEPYTKE YAAESEECMT VRFGSFDKNS RVVLIDDVIA TGGTMLAGVQ LVDACGATLV EVAGILGLTF LKGTQPAHTF AGGRYSNVPF VTLVDETVLS DENCGDPLHH KGSRIISCAE AKKLI
NT Sequence
atgtcacttg tggaggtttt gccgaactac ttcactcttt cgaaggactc accccttcgg aagaagttcg aaaaggtgta caaatggtat tcaccagcct tttcgcctca cgatgttccg cgctttgctg aggtggggaa tatcaccgaa aatcctgaag tgatgcgcgg cattcgagac ttcttcgttg accgctataa aaatcttcag cagcccatca cccacatttt ggggtttgac tctcgtggtt ttctgcttgg accaatgatt gcagtggagc tgaacgtgcc ttttgtactg ataaggaagg cgaacaaaat cgctggtgtt atcattaaga gtgaacctta cacgaaggaa tacgccgcag aatcagagga gtgcatgacc gttcgcttcg ggagctttga caagaattct cgcgttgttc tcatcgatga tgtgattgcc actggtggta ccatgcttgc gggagtgcaa cttgttgatg catgtggtgc cactcttgtg gaagtggcgg gtattctcgg cctcactttc ctcaaaggaa cgcaacccgc ccatacattt gcgggaggtc ggtacagtaa tgttccattt gtcactcttg tggatgaaac tgtcctttct gatgaaaact gtggcgatcc tcttcatcac aagggatccc gcataataag ctgcgccgag gcaaaaaaat tgatttaa
Details for TrbrA.01405.a.B1.GE40765
| HARVESTED ON: | 11/21/2016 |
| SEQUENCED ON: | 11/22/2016 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS LVEVLPNYFT LSKDSPLRKK FEKVYKWYSP AFSPHDVPRF AEVGNITENP EVMRGIRDFF VDRYKNLQQP ITHILGFDSR GFLLGPMIAV ELNVPFVLIR KANKIAGVII KSEPYTKEYA AESEECMTVR FGSFDKNSRV VLIDDVIATG GTMLAGVQLV DACGATLVEV AGILGLTFLK GTQPAHTFAG GRYSNVPFVT LVDETVLSDE NCGDPLHHKG SRIISCAEAK KLI
Validated NT Sequence
atcttactca cttaaatcaa tttttttgcc tcggcgcagc ttattatgcg ggatcccttg tgatgaagag gatcgccaca gttttcatca gaaaggacag tttcatccac aagagtgaca aatggaacat tactgtaccg acctcccgca aatgtatggg cgggttgcgt tcctttgagg aaagtgaggc cgagaatacc cgccacttcc acaagagtgg caccacatgc atcaacaagt tgcactcccg caagcatggt accaccagtg gcaatcacat catcgatgag aacaacgcga gaattcttgt caaagctccc gaagcgaacg gtcatgcact cctctgattc tgcggcgtat tccttcgtgt aaggttcact cttaatgata acaccagcga ttttgttcgc cttccttatc agtacaaaag gcacgttcag ctccactgca atcattggtc caagcagaaa accacgagag tcaaacccca aaatgtgggt gatgggctgc tgaagatttt tatagcggtc aacgaagaag tctcgaatgc cgcgcatcac ttcaggattt tcggtgatat tccccacctc agcaaagcgc ggaacatcgt gaggcgaaaa ggctggtgaa taccatttgt acaccttttc gaacttcttc cgaaggggtg agtccttcga aagagtgaag tagttcggca aaacctccac aagtgacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS LVEVLPNYFT LSKDSPLRKK FEKVYKWYSP AFSPHDVPRF AEVGNITENP EVMRGIRDFF VDRYKNLQQP ITHILGFDSR GFLLGPMIAV ELNVPFVLIR KANKIAGVII KSEPYTKEYA AESEECMTVR FGSFDKNSRV VLIDDVIATG GTMLAGVQLV DACGATLVEV AGILGLTFLK GTQPAHTFAG GRYSNVPFVT LVDETVLSDE NCGDPLHHKG SRIISCAEAK KLI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcac ttgtggaggt tttgccgaac tacttcactc tttcgaagga ctcacccctt cggaagaagt tcgaaaaggt gtacaaatgg tattcaccag ccttttcgcc tcacgatgtt ccgcgctttg ctgaggtggg gaatatcacc gaaaatcctg aagtgatgcg cggcattcga gacttcttcg ttgaccgcta taaaaatctt cagcagccca tcacccacat tttggggttt gactctcgtg gttttctgct tggaccaatg attgcagtgg agctgaacgt gccttttgta ctgataagga aggcgaacaa aatcgctggt gttatcatta agagtgaacc ttacacgaag gaatacgccg cagaatcaga ggagtgcatg accgttcgct tcgggagctt tgacaagaat tctcgcgttg ttctcatcga tgatgtgatt gccactggtg gtaccatgct tgcgggagtg caacttgttg atgcatgtgg tgccactctt gtggaagtgg cgggtattct cggcctcact ttcctcaaag gaacgcaacc cgcccataca tttgcgggag gtcggtacag taatgttcca tttgtcactc ttgtggatga aactgtcctt tctgatgaaa actgtggcga tcctcttcat cacaagggat cccgcataat aagctgcgcc gaggcaaaaa aattgatttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for TrbrA.01405.a.B1.PS38186
| PURIFICATION DATe: | 2/1/2017 |
| CONCENTRATION: | 45.7mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS LVEVLPNYFT LSKDSPLRKK FEKVYKWYSP AFSPHDVPRF AEVGNITENP EVMRGIRDFF VDRYKNLQQP ITHILGFDSR GFLLGPMIAV ELNVPFVLIR KANKIAGVII KSEPYTKEYA AESEECMTVR FGSFDKNSRV VLIDDVIATG GTMLAGVQLV DACGATLVEV AGILGLTFLK GTQPAHTFAG GRYSNVPFVT LVDETVLSDE NCGDPLHHKG SRIISCAEAK KLI
Validated NT Sequence
atcttactca cttaaatcaa tttttttgcc tcggcgcagc ttattatgcg ggatcccttg tgatgaagag gatcgccaca gttttcatca gaaaggacag tttcatccac aagagtgaca aatggaacat tactgtaccg acctcccgca aatgtatggg cgggttgcgt tcctttgagg aaagtgaggc cgagaatacc cgccacttcc acaagagtgg caccacatgc atcaacaagt tgcactcccg caagcatggt accaccagtg gcaatcacat catcgatgag aacaacgcga gaattcttgt caaagctccc gaagcgaacg gtcatgcact cctctgattc tgcggcgtat tccttcgtgt aaggttcact cttaatgata acaccagcga ttttgttcgc cttccttatc agtacaaaag gcacgttcag ctccactgca atcattggtc caagcagaaa accacgagag tcaaacccca aaatgtgggt gatgggctgc tgaagatttt tatagcggtc aacgaagaag tctcgaatgc cgcgcatcac ttcaggattt tcggtgatat tccccacctc agcaaagcgc ggaacatcgt gaggcgaaaa ggctggtgaa taccatttgt acaccttttc gaacttcttc cgaaggggtg agtccttcga aagagtgaag tagttcggca aaacctccac aagtgacata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMS LVEVLPNYFT LSKDSPLRKK FEKVYKWYSP AFSPHDVPRF AEVGNITENP EVMRGIRDFF VDRYKNLQQP ITHILGFDSR GFLLGPMIAV ELNVPFVLIR KANKIAGVII KSEPYTKEYA AESEECMTVR FGSFDKNSRV VLIDDVIATG GTMLAGVQLV DACGATLVEV AGILGLTFLK GTQPAHTFAG GRYSNVPFVT LVDETVLSDE NCGDPLHHKG SRIISCAEAK KLI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAC TTGTGGAGGT TTTGCCGAAC TACTTCACTC TTTCGAAGGA CTCACCCCTT CGGAAGAAGT TCGAAAAGGT GTACAAATGG TATTCACCAG CCTTTTCGCC TCACGATGTT CCGCGCTTTG CTGAGGTGGG GAATATCACC GAAAATCCTG AAGTGATGCG CGGCATTCGA GACTTCTTCG TTGACCGCTA TAAAAATCTT CAGCAGCCCA TCACCCACAT TTTGGGGTTT GACTCTCGTG GTTTTCTGCT TGGACCAATG ATTGCAGTGG AGCTGAACGT GCCTTTTGTA CTGATAAGGA AGGCGAACAA AATCGCTGGT GTTATCATTA AGAGTGAACC TTACACGAAG GAATACGCCG CAGAATCAGA GGAGTGCATG ACCGTTCGCT TCGGGAGCTT TGACAAGAAT TCTCGCGTTG TTCTCATCGA TGATGTGATT GCCACTGGTG GTACCATGCT TGCGGGAGTG CAACTTGTTG ATGCATGTGG TGCCACTCTT GTGGAAGTGG CGGGTATTCT CGGCCTCACT TTCCTCAAAG GAACGCAACC CGCCCATACA TTTGCGGGAG GTCGGTACAG TAATGTTCCA TTTGTCACTC TTGTGGATGA AACTGTCCTT TCTGATGAAA ACTGTGGCGA TCCTCTTCAT CACAAGGGAT CCCGCATAAT AAGCTGCGCC GAGGCAAAAA AATTGATTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT