TrbrA.01391.c

Protein kinase, putative (EC 2.7.1.-) (CRK12)

CENTER ID: TrbrA.01391.c
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.01391.c.B1.GE42087 full length 1 769
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tb11.01.4130
UniProt: Q382V0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGMATRSPSP RTVHSGQRQE EGNWVASVPG ERTQLGTSDH VKLHPIDRCS PDIPISPPHT SAADVNDVPL AEVVHGGGFG ETNIAADIGG CARLVEPATF CVACEDRQST TLLLAHNQNA LDHQKQQQPV DGEGQCPLTA HDGPPRGRLR FGLRSLRVVT EESGTTARRF LRLRRPQVGA EERAEQSIGP GDRHFAPEEG EHPLQEKTNS QFDTQPLPPM HTSVTTDGSS RTNEKVEHPG GTVQHQHTTR MAEAVNASFN GPTPSLSVCK LGKSLGSGVL LSPTPTTPIL KRNNGAASRK RHSKVNVFLP HSPVPIDFSS VTTHVESKYE VGKKISEGTY GEVYIGRCRT TGEYVALKRL KVLEGLEGFP ITSLREVIAL QHINNERQNI AVRNGNASGS NRAKMDAIDE VVRLRDVLLS STHNDIYLVF PYASCSLAGL MHRRFPFSEK EIAYIFRKVI TAVKKLHEMG IIHRDVKADN VLINRDGSVQ LGDFGLCAFE GCGTRALTPS LINLNYRPPE MLLGAVAYNA KVDIWSIGCF LSQMFLRVPP FACVRPKAEF DGAGAGNAKV DPHPKEPQQK QKLKQQGYRV QAETELAQLS LITEVLGPLG GDLDEAFPPA QCRNVSLLRE VRASLTSCNS QSALRASMAS LFEPSHLYSQ YRGFRSWFIA TAERRRRSPT YPAPSPECLD VLTAIFQLDP RKRPTAAQLL EMPFFDLSRA VSSPARRSYA AYTYAEVEVE KAELLIREEM AEKLQRYEGS HLLPYPSAA
NT Sequence
atgggtatgg caacacgttc gccttcgcca cggacagttc actccggaca gcggcaggag gaagggaatt gggtggcgtc tgttcccggg gagagaactc aactagggac atcggatcac gtgaagctcc acccaattga tcgctgctct cccgatatcc ccatatcgcc gcctcacacc tcagccgctg atgtgaatga tgtaccttta gcggaggttg ttcacggtgg gggcttcggt gaaactaata ttgccgccga tattggaggt tgtgctcgct tagttgagcc tgcaactttt tgtgtggcct gcgaagaccg ccaaagtacc acccttttgt tagctcacaa ccagaacgct ttggaccacc aaaagcaaca gcaacccgtg gatggtgagg gacagtgccc actcaccgcg cacgatgggc caccacgagg caggttgcga tttggtttgc gttcgttgag ggttgtaacc gaagaaagtg gcacgacggc gaggcggttc ttaagactta gaagaccaca agtaggtgcc gaggagcggg ctgaacagtc cataggtcca ggagacagac atttcgctcc agaagaggga gaacatcccc ttcaagaaaa aacaaattca caattcgaca cccagcccct tccccctatg cacaccagcg taactacaga cggatcatcc cgcaccaacg aaaaggtgga gcatcccggt ggtactgtgc aacaccagca cacgacaagg atggcggagg cagtaaatgc aagcttcaat ggccccactc cgtcgctttc ggtgtgtaaa ttgggaaagt ctttgggatc tggtgtactg ctctcaccaa cacctacgac cccaatattg aagagaaata atggtgcggc cagccgaaag cgtcacagca aggtgaatgt atttctgccg catagtcccg ttcccatcga cttcagttcg gttaccacac atgtggagtc aaagtacgaa gttgggaaaa agataagtga gggcacgtac ggtgaagtat acattggtcg ttgccgaact acgggtgagt atgttgcgtt gaagcgactt aaagttttag aggggctgga ggggtttccc atcacgtctc tgcgagaagt gatcgccctt cagcacatta acaacgagcg ccaaaacatt gcagtacgta acggaaacgc aagcggcagc aaccgtgcca aaatggacgc catagatgaa gtagttagac tccgtgacgt tttactgtcg agcacccaca acgatatata tcttgtgttc ccttacgcgt cttgcagttt ggctgggcta atgcaccgcc gatttccatt cagtgagaaa gagattgcgt atattttccg gaaagttatt actgctgtga aaaagttaca tgaaatgggt atcatccacc gtgatgtcaa ggctgacaat gtgctcatta atagggatgg cagcgtccag cttggggact tcggtttgtg cgcctttgag ggatgcggca cgcgtgcctt aactcccagt cttattaacc ttaactatcg cccgcctgag atgttactcg gtgccgtcgc atataacgca aaggttgata tatggtctat tggctgcttt ctaagccaaa tgtttctgcg tgtgccgcca tttgcctgcg ttcggccgaa agcagaattc gacggagcgg gcgctggcaa cgcaaaggtg gacccgcatc ccaaagagcc gcagcaaaaa cagaagttaa aacagcaggg gtatagggtg caagcggaaa cagaactggc gcaactatct cttataacag aggtgttggg cccgcttggc ggtgaccttg atgaagcttt cccaccggcg cagtgtcgaa acgtgtcgct attacgtgag gtacgcgcat ctctaactag ctgtaactca caatcagcgt taagggcgtc tatggcttct ctttttgaac caagccatct ttactctcag tacaggggct tccgctcgtg gttcatcgcc actgctgagc gccgccgtcg ttccccaact tatccagcgc cgtcgccgga gtgtctcgac gtgctcactg caatatttca gctggatccg cggaaacggc cgacggcggc ccaattgctg gaaatgcctt tctttgacct tagccgcgcg gtgtcgtctc ccgcccgacg gtcgtatgct gcttatactt acgctgaagt tgaggtagag aaggcggagc tgttaataag agaagaaatg gctgagaaat tacagaggta cgagggcagt caccttttgc cttatccaag cgctgcgtaa
Details for TrbrA.01391.c.B1.GE42087
HARVESTED ON: data unavailable
SEQUENCED ON: 12/7/2017
EXPECTED MW: 86kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG MATRSPSPRT VHSGQRQEEG NWVASVPGER TQLGTSDHVK LHPIDRCSPD IPISPPHTSA ADVNDVPLAE VVHGGGFGET NIAADIGGCA RLVEPATFCV ACEDRQSTTL LLAHNQNALD HQKQQQPVDG EGQCPLTAHD GPPRGRLRFG LRSLRVVTEE SGTTARRFLR LRRPQVGAEE RAEQSIGPGD RHFAPEEGEH PLQEKTNSQF DTQPLPPMHT SVTTDGSSRT NEKVEHPGGT VQHQHTTRMA EAVNASFNGP TPSLSVCKLG KSLGSGVLLS PTPTTPILKR NNGAASRKRH SKVNVFLPHS PVPIDFSSVT THVESKYEVG KKISEGTYGE VYIGRCRTTG EYVALKRLKV LEGLEGFPIT SLREVIALQH INNERQNIAV RNGNASGSNR AKMDAIDEVV RLRDVLLSST HNDIYLVFPY ASCSLAGLMH RRFPFSEKEI AYIFRKVITA VKKLHEMGII HRDVKADNVL INRDGSVQLG DFGLCAFEGC GTRALTPSLI NLNYRPPEML LGAVAYNAKV DIWSIGCFLS QMFLRVPPFA CVRPKAEFDG AGAGNAKVDP HPKEPQQKQK LKQQGYRVQA ETELAQLSLI TEVLGPLGGD LDEAFPPAQC RNVSLLREVR ASLTSCNSQS ALRASMASLF EPSHLYSQYR GFRSWFIATA ERRRRSPTYP APSPECLDVL TAIFQLDPRK RPTAAQLLEM PFFDLSRAVS SPARRSYAAY TYAEVEVEKA ELLIREEMAE KLQRYEGSHL LPYPSAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta tggcaacacg ttcgccttcg ccacggacag ttcactccgg acagcggcag gaggaaggga attgggtggc gtctgttccc ggggagagaa ctcaactagg gacatcggat cacgtgaagc tccacccaat tgatcgctgc tctcccgata tccccatatc gccgcctcac acctcagccg ctgatgtgaa tgatgtacct ttagcggagg ttgttcacgg tgggggcttc ggtgaaacta atattgccgc cgatattgga ggttgtgctc gcttagttga gcctgcaact ttttgtgtgg cctgcgaaga ccgccaaagt accacccttt tgttagctca caaccagaac gctttggacc accaaaagca acagcaaccc gtggatggtg agggacagtg cccactcacc gcgcacgatg ggccaccacg aggcaggttg cgatttggtt tgcgttcgtt gagggttgta accgaagaaa gtggcacgac ggcgaggcgg ttcttaagac ttagaagacc acaagtaggt gccgaggagc gggctgaaca gtccataggt ccaggagaca gacatttcgc tccagaagag ggagaacatc cccttcaaga aaaaacaaat tcacaattcg acacccagcc ccttccccct atgcacacca gcgtaactac agacggatca tcccgcacca acgaaaaggt ggagcatccc ggtggtactg tgcaacacca gcacacgaca aggatggcgg aggcagtaaa tgcaagcttc aatggcccca ctccgtcgct ttcggtgtgt aaattgggaa agtctttggg atctggtgta ctgctctcac caacacctac gaccccaata ttgaagagaa ataatggtgc ggccagccga aagcgtcaca gcaaggtgaa tgtatttctg ccgcatagtc ccgttcccat cgacttcagt tcggttacca cacatgtgga gtcaaagtac gaagttggga aaaagataag tgagggcacg tacggtgaag tatacattgg tcgttgccga actacgggtg agtatgttgc gttgaagcga cttaaagttt tagaggggct ggaggggttt cccatcacgt ctctgcgaga agtgatcgcc cttcagcaca ttaacaacga gcgccaaaac attgcagtac gtaacggaaa cgcaagcggc agcaaccgtg ccaaaatgga cgccatagat gaagtagtta gactccgtga cgttttactg tcgagcaccc acaacgatat atatcttgtg ttcccttacg cgtcttgcag tttggctggg ctaatgcacc gccgatttcc attcagtgag aaagagattg cgtatatttt ccggaaagtt attactgctg tgaaaaagtt acatgaaatg ggtatcatcc accgtgatgt caaggctgac aatgtgctca ttaataggga tggcagcgtc cagcttgggg acttcggttt gtgcgccttt gagggatgcg gcacgcgtgc cttaactccc agtcttatta accttaacta tcgcccgcct gagatgttac tcggtgccgt cgcatataac gcaaaggttg atatatggtc tattggctgc tttctaagcc aaatgtttct gcgtgtgccg ccatttgcct gcgttcggcc gaaagcagaa ttcgacggag cgggcgctgg caacgcaaag gtggacccgc atcccaaaga gccgcagcaa aaacagaagt taaaacagca ggggtatagg gtgcaagcgg aaacagaact ggcgcaacta tctcttataa cagaggtgtt gggcccgctt ggcggtgacc ttgatgaagc tttcccaccg gcgcagtgtc gaaacgtgtc gctattacgt gaggtacgcg catctctaac tagctgtaac tcacaatcag cgttaagggc gtctatggct tctctttttg aaccaagcca tctttactct cagtacaggg gcttccgctc gtggttcatc gccactgctg agcgccgccg tcgttcccca acttatccag cgccgtcgcc ggagtgtctc gacgtgctca ctgcaatatt tcagctggat ccgcggaaac ggccgacggc ggcccaattg ctggaaatgc ctttctttga ccttagccgc gcggtgtcgt ctcccgcccg acggtcgtat gctgcttata cttacgctga agttgaggta gagaaggcgg agctgttaat aagagaagaa atggctgaga aattacagag gtacgagggc agtcaccttt tgccttatcc aagcgctgcg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat