TrbrA.01391.a

protein kinase, putative,cdc2-related related kinase, putative

CENTER ID: TrbrA.01391.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.01391.a.A1.GE34294 full length 1 292
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.7.1900
RefSeq: XP_845814.1
UniProt: Q57V20

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKYLLGPSM GEGRFGSVRS AVIRGSGVPV AIKLINVTRV EEGIPHMVAR ELLVSMRLQH PYIIRTYEVF PYGSSVALVM ERCATDLSAV LCERSPANPL SPQSAKSLFR MLLGALAYMH KEGILHRDVK PSNCFLARDG TLRLGDFGLS RMQDTEESMT HEVVSRWYRP PELLLGQRHY GPEVDMWSAG CVFVELLRGY TGAFFASDGD VMQLSRIFDV LGTPTQESWP TAELLPDWGK VHFEPKNPCP LGEFFPEASG SAVDLLRGLL CLDPSRRLSA ADALCHTYFN EY
NT Sequence
atggagaagt acctccttgg cccttcgatg ggagaaggaa ggttcggatc cgtacgttcc gctgttatca gggggtccgg ggtgcctgtc gccatcaagt tgattaacgt cacgcgcgtt gaggaaggta ttcctcatat ggttgccagg gaattactag tgtctatgcg gctgcagcac ccatacatta ttaggactta tgaggtcttc ccatacggat cttctgtggc tttggtgatg gagcgttgcg cgaccgattt gtcggcggtg ctctgtgaaa gaagtcccgc taatcccctt tcccctcaat cagcaaaatc acttttcagg atgcttcttg gggcacttgc atatatgcac aaggagggca tccttcatcg tgacgtaaaa ccctcaaatt gcttcttggc gcgagatgga acccttcgtc ttggtgactt tggtcttagt cgcatgcaag acacggaaga gtccatgact catgaggtgg tgtcgagatg gtaccgtccc ccggagttgt tgctgggtca gcgccactac ggaccagagg ttgacatgtg gtccgctggc tgtgtgttcg tagagttgtt acgagggtat accggcgcgt tttttgctag tgacggagat gtcatgcagc tctcacggat tttcgatgtt ttgggcaccc cgacacagga atcatggccc actgcggagt tgcttccaga ttggggaaag gtgcacttcg aacctaagaa cccgtgccca ttgggtgagt ttttccccga ggcgtccgga tctgcggttg acctattgcg gggtttactt tgtcttgatc cttccagacg tctcagtgct gcagatgccc tttgtcatac atatttcaac gagtactga
Details for TrbrA.01391.a.A1.GE34294
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEKYLLGPS MGEGRFGSVR SAVIRGSGVP VAIKLINVTR VEEGIPHMVA RELLVSMRLQ HPYIIRTYEV FPYGSSVALV MERCATDLSA VLCERSPANP LSPQSAKSLF RMLLGALAYM HKEGILHRDV KPSNCFLARD GTLRLGDFGL SRMQDTEESM THEVVSRWYR PPELLLGQRH YGPEVDMWSA GCVFVELLRG YTGAFFASDG DVMQLSRIFD VLGTPTQESW PTAELLPDWG KVHFEPXNPC PLGEFFPEAS GSAVDLLRGL LCLDPSRRLS AADAXCXTYF XXX
Validated NT Sequence
ttttgtttaa ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaga agtacctcct tggcccttcg atgggagaag gaaggttcgg atccgtacgt tccgctgtta tcagggggtc cggggtgcct gtcgccatca agttgattaa cgtcacgcgc gttgaggaag gtattcctca tatggttgcc agggaattac tagtgtctat gcggctgcag cacccataca ttattaggac ttatgaggtc ttcccatacg gatcttctgt ggctttggtg atggagcgtt gcgcgaccga tttgtcggcg gtgctctgtg aaagaagtcc cgctaatccc ctttcccctc aatcagcaaa atcacttttc aggatgcttc ttggggcact tgcatatatg cacaaggagg gcatccttca tcgtgacgta aaaccctcaa attgcttctt ggcgcgagat ggaacccttc gtcttggtga ctttggtctt agtcgcatgc aagacacgga agagtccatg actcatgagg tggtgtcgag atggtaccgt cccccggagt tgttgctggg tcagcgccac tacggaccag aggttgacat gtggtccgct ggctgtgtgt tcgtagagtt gttacgaggg tataccggcg cgttttttgc tagtgacgga gatgtcatgc agctctcacg gattttcgat gttttgggca ccccgacaca ggaatcatgg cccactgcgg agttgcttcc agattgggga aaggtgcact tcgaacctaa naacccgtgc ccattgggtg agtttttccc cgaggcgtcc ggatctgcgg ttgacctatt gcggggttta ctttgtcttg atccttccag acgtctcagt gctgcagatg ccntttgtcn tacatatttc ancnagnact aataaangca cnannantnc tgcagcnnnc ttctcnnnnn nncnnntgct ancnaagcnn naannnanct nnnnnnnntg ctnnnnnnnn t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEKYLLGPS MGEGRFGSVR SAVIRGSGVP VAIKLINVTR VEEGIPHMVA RELLVSMRLQ HPYIIRTYEV FPYGSSVALV MERCATDLSA VLCERSPANP LSPQSAKSLF RMLLGALAYM HKEGILHRDV KPSNCFLARD GTLRLGDFGL SRMQDTEESM THEVVSRWYR PPELLLGQRH YGPEVDMWSA GCVFVELLRG YTGAFFASDG DVMQLSRIFD VLGTPTQESW PTAELLPDWG KVHFEPKNPC PLGEFFPEAS GSAVDLLRGL LCLDPSRRLS AADALCHTYF NEY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagaagtac ctccttggcc cttcgatggg agaaggaagg ttcggatccg tacgttccgc tgttatcagg gggtccgggg tgcctgtcgc catcaagttg attaacgtca cgcgcgttga ggaaggtatt cctcatatgg ttgccaggga attactagtg tctatgcggc tgcagcaccc atacattatt aggacttatg aggtcttccc atacggatct tctgtggctt tggtgatgga gcgttgcgcg accgatttgt cggcggtgct ctgtgaaaga agtcccgcta atcccctttc ccctcaatca gcaaaatcac ttttcaggat gcttcttggg gcacttgcat atatgcacaa ggagggcatc cttcatcgtg acgtaaaacc ctcaaattgc ttcttggcgc gagatggaac ccttcgtctt ggtgactttg gtcttagtcg catgcaagac acggaagagt ccatgactca tgaggtggtg tcgagatggt accgtccccc ggagttgttg ctgggtcagc gccactacgg accagaggtt gacatgtggt ccgctggctg tgtgttcgta gagttgttac gagggtatac cggcgcgttt tttgctagtg acggagatgt catgcagctc tcacggattt tcgatgtttt gggcaccccg acacaggaat catggcccac tgcggagttg cttccagatt ggggaaaggt gcacttcgaa cctaagaacc cgtgcccatt gggtgagttt ttccccgagg cgtccggatc tgcggttgac ctattgcggg gtttactttg tcttgatcct tccagacgtc tcagtgctgc agatgccctt tgtcatacat atttcaacga gtactaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa