SSGCID: Target Page

TrbrA.01375.b

cyclophilin, putative

CENTER ID:	TrbrA.01375.b
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.01375.b.A2.GE34293	mature protein		24	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.01375.b.A2.PS01663	mature protein		24	301

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.8.2000
OrthoMCL:	OG5_126792
RefSeq:	XP_847033.1
UniProt:	Q581Y2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKSPQNFRK CGLLFVSTFD FLYACNLFPP VLFSSGKESM PKVSVPPKTE RHGKIEAPET NNPKVFFDVS INEKAAGRIV MELYADTVPR TAENFRALCT GEKGRGKSGK PLHYKGCIFH RVIPGFMLQG GDITRGNGTG GESIYGTTFR DESFAGKAGK HTGLGCLSMA NAGPNTNGSQ FFICTANTPW LNGKHVVFGR VTEGIDVVKS IERLGSDSGK TRGRIIIANC GELQTQKEGA AKKEKVKAKE GNNAGKLSTI VEGTGGAKRP REDVEDLEER KKRIREKRER IAKLRAQAEE K

NT Sequence

atgctaaaga gcccgcaaaa ttttcggaag tgtggtctgt tatttgtgtc aacgtttgat tttttgtatg cgtgtaacct ctttcccccc gttttatttt cttcaggtaa ggagtcaatg cccaaggtaa gcgtcccacc taagacggag cgccacggga agatcgaggc gccagagact aataatccca aagtcttctt tgatgtgtct ataaatgaga aagcagcggg acggattgtt atggaattgt acgcggacac tgtgccgagg acagcggaga atttccgtgc actatgcacg ggcgagaagg gtcgtgggaa gagtggaaag ccgctgcatt acaaggggtg cattttccac cgagttatac caggtttcat gctacaaggg ggtgacatca cacgtggaaa tggcaccggt ggagagtcca tatacggcac aacgtttcgt gacgagagtt ttgccggaaa agccggaaag cataccggac tcggttgcct gtccatggct aacgcgggac ccaacaccaa cggttcgcag tttttcatct gtacagccaa cacaccgtgg ttgaacggaa agcacgttgt gtttggtcgt gttactgagg gaattgacgt ggtgaagagc attgaacggt tgggttccga ttcggggaaa acgcgcggtc gaatcatcat cgcgaattgt ggtgagctgc agacgcagaa ggaaggtgca gccaaaaagg agaaggttaa ggcgaaggaa ggaaacaatg cagggaagct gtccaccatt gtcgaaggga ccggtggagc aaagcgtcca cgtgaagatg tggaggattt ggaagaacgg aagaaacgca tccgagagaa gagggagagg atagcgaaac tccgcgccca ggcggaagag aaataa

Details for TrbrA.01375.b.A2.GE34293

HARVESTED ON:	12/19/2011
SEQUENCED ON:	12/28/2011
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMACNLFPPV LFSSGKESMP KVSVPPKTER HGKIEAPETN NLKVFFDVSI NEKAAGRIVM ELYADTVPRT AENFRALCTG EKGRGKSGKP LHYKGCIFHR VIPGFMLQGG DITRGNGTGG ESIYGTTFRD ESFAGKAGKH TGLGCLSMAN AGPNTNGSQF FICTANTPWL NGKHVVFGRV TEGIDVVKSI ERLGSDSGKT RGRIIIANCG ELQTQKEGAA KKEKVKAKEG NNAGKLSTIV EGTGGAKRPR EDVEDLEERK KRIREKRERI AKLRAQAE

Validated NT Sequence

cttccgcctg ggcgcggagt ttcgctatcc tctccctctt ctctcggatg cgtttcttcc gttcttccaa atcctccaca tcttcacgtg gacgctttgc tccaccggtc ccttcgacaa tggtggacag cttccctgca ttgtttcctt ccttcgcctt aaccttctcc tttttagctg caccttcctt ctgcgtctgc agctcaccac aattcgcgat gatgattcga ccgcgcgttt tccccgaatc ggaacccaac cgttcaatgc tcttcaccac gtcaattccc tcagtaacac gaccaaacac aacgtgcttt ccattcaacc acggtgtgtt ggctgtacag atgaaaaact gcgaaccgtt ggtgttgggt cccgcgttag ccatggacag gcaaccgagt ccggtatgct ttccggcttt tccggcaaaa ctctcgtcac gaaacgttgt gccgtatatg gactctccac cggtgccatt tccacgtgtg atgtcacccc cttgtagcat gaaacctggt atgactcggt ggaaaatgca ccccttgtaa tgcagcggct ttccactctt cccacgaccc ttctcgcctg tgcatagtgc acggaaattc tccgctgtcc tcggcacagt gtccgcgtac aattccataa caatccgtcc cgctgctttc tcatttatag acacatcaaa gaagactttg agattattag tctctggcgc ctcgatcttc ccgtggcgct ccgtcttagg tgggacgctt accttgggca ttgactcctt acctgaagaa aataaaacgg ggggaaagag gttacacgcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccatggta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMACNLFPPV LFSSGKESMP KVSVPPKTER HGKIEAPETN NPKVFFDVSI NEKAAGRIVM ELYADTVPRT AENFRALCTG EKGRGKSGKP LHYKGCIFHR VIPGFMLQGG DITRGNGTGG ESIYGTTFRD ESFAGKAGKH TGLGCLSMAN AGPNTNGSQF FICTANTPWL NGKHVVFGRV TEGIDVVKSI ERLGSDSGKT RGRIIIANCG ELQTQKEGAA KKEKVKAKEG NNAGKLSTIV EGTGGAKRPR EDVEDLEERK KRIREKRERI AKLRAQAEEK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtgtaac ctctttcccc ccgttttatt ttcttcaggt aaggagtcaa tgcccaaggt aagcgtccca cctaagacgg agcgccacgg gaagatcgag gcgccagaga ctaataatcc caaagtcttc tttgatgtgt ctataaatga gaaagcagcg ggacggattg ttatggaatt gtacgcggac actgtgccga ggacagcgga gaatttccgt gcactatgca cgggcgagaa gggtcgtggg aagagtggaa agccgctgca ttacaagggg tgcattttcc accgagttat accaggtttc atgctacaag ggggtgacat cacacgtgga aatggcaccg gtggagagtc catatacggc acaacgtttc gtgacgagag ttttgccgga aaagccggaa agcataccgg actcggttgc ctgtccatgg ctaacgcggg acccaacacc aacggttcgc agtttttcat ctgtacagcc aacacaccgt ggttgaacgg aaagcacgtt gtgtttggtc gtgttactga gggaattgac gtggtgaaga gcattgaacg gttgggttcc gattcgggga aaacgcgcgg tcgaatcatc atcgcgaatt gtggtgagct gcagacgcag aaggaaggtg cagccaaaaa ggagaaggtt aaggcgaagg aaggaaacaa tgcagggaag ctgtccacca ttgtcgaagg gaccggtgga gcaaagcgtc cacgtgaaga tgtggaggat ttggaagaac ggaagaaacg catccgagag aagagggaga ggatagcgaa actccgcgcc caggcggaag agaaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for TrbrA.01375.b.A2.PS01663

PURIFICATION DATe:	10/30/2012
CONCENTRATION:	89mg/ml
OBSERVED MW:	32kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGTGTAAC CTCTTTCCCC CCGTTTTATT TTCTTCAGGT AAGGAGTCAA TGCCCAAGGT AAGCGTCCCA CCTAAGACGG AGCGCCACGG GAAGATCGAG GCGCCAGAGA CTAATAATCC CAAAGTCTTC TTTGATGTGT CTATAAATGA GAAAGCAGCG GGACGGATTG TTATGGAATT GTACGCGGAC ACTGTGCCGA GGACAGCGGA GAATTTCCGT GCACTATGCA CGGGCGAGAA GGGTCGTGGG AAGAGTGGAA AGCCGCTGCA TTACAAGGGG TGCATTTTCC ACCGAGTTAT ACCAGGTTTC ATGCTACAAG GGGGTGACAT CACACGTGGA AATGGCACCG GTGGAGAGTC CATATACGGC ACAACGTTTC GTGACGAGAG TTTTGCCGGA AAAGCCGGAA AGCATACCGG ACTCGGTTGC CTGTCCATGG CTAACGCGGG ACCCAACACC AACGGTTCGC AGTTTTTCAT CTGTACAGCC AACACACCGT GGTTGAACGG AAAGCACGTT GTGTTTGGTC GTGTTACTGA GGGAATTGAC GTGGTGAAGA GCATTGAACG GTTGGGTTCC GATTCGGGGA AAACGCGCGG TCGAATCATC ATCGCGAATT GTGGTGAGCT GCAGACGCAG AAGGAAGGTG CAGCCAAAAA GGAGAAGGTT AAGGCGAAGG AAGGAAACAA TGCAGGGAAG CTGTCCACCA TTGTCGAAGG GACCGGTGGA GCAAAGCGTC CACGTGAAGA TGTGGAGGAT TTGGAAGAAC GGAAGAAACG CATCCGAGAG AAGAGGGAGA GGATAGCGAA ACTCCGCGCC CAGGCGGAAG AGAAATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA