SSGCID: Target Page

TrbrA.01375.a

cyclophilin, putative

CENTER ID:	TrbrA.01375.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.01375.a.A1.GE34297	full length		1	243

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.8.5230
RefSeq:	XP_847353.1
UniProt:	Q57UJ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MELACFNGFP ETPSLSGSLV QQRHNLLDSV KSAGPPGPVF RVEFELFDDE SPKTCANFRN LCAGRTTSRK GETYWFQGVV PSYRGTYFHK IIPQFCAQGG DLTMRVDNGA NHLSSFGRGW FADEYKRRRM NEVGLLAMAN NGPNSNGSQF FITTSDSNEK ALNGRHVCFG RVVQGLKEFM HEVAPFGDSA GYPSRFIVVT DCGEGPLPAT LGSTLPDKTP AADISEEGAR ECGRVSVKSP SGG

NT Sequence

atggagctcg catgttttaa cggtttccca gaaaccccat ccctctccgg gtctttagtt cagcaacgtc ataatttgct ggactctgtc aaatccgcgg ggcctccggg gcctgtcttt cgcgtggaat ttgaactttt tgatgatgaa tcgcccaaga cttgtgcgaa cttcaggaat ttgtgcgcag ggcgcacaac cagccggaag ggtgagacat actggttcca gggtgtcgtg ccttcctacc gcgggacata ctttcacaag attattccac aattctgcgc tcaaggcggg gacctcacca tgcgggtaga caatggcgcg aatcatttga gtagcttcgg tcgcggttgg ttcgcggatg agtataagcg acggcggatg aacgaagtcg ggctcttggc gatggccaac aacggaccca acagcaacgg ctcgcaattt ttcataacca cgtctgactc aaatgagaag gcgctcaacg gtcgtcacgt ttgctttggc cgagtggtgc agggcctgaa ggagttcatg catgaggttg ctccctttgg cgacagtgct ggctatccat cacgttttat tgtcgttacg gactgcgggg aagggccact accagctacg ctcggctcca cattgccgga taaaacacct gcagctgata ttagcgaaga aggagctcgg gaatgcggga gggtgtcggt gaagtcccct tccggcgggt ag

Details for TrbrA.01375.a.A1.GE34297

HARVESTED ON:	12/19/2011
SEQUENCED ON:	12/28/2011
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMELACFNGF PETPSLSGSL VQQRHNLLDS VKSAGPPGPV FRVEFELFDD ESPKTCANFR NLCAGRTTSR KGETYWFQGV VPSYRGTYFH KIIPQFCAQG GDLTMRVDNG ANHLSSFGRG WFADEYKRRR MNEVGLLAMA NNGPNSNGSQ FFITTSDSNE KALNGRHVCF GRVVQGLKEF MHEVAPFGDS AGYPSRFIVV TDCGEGPLPA TLGSTLPDKT PAADISEEGA REGGRVSVKS PSGG

Validated NT Sequence

ttattacccg ccggaagggg acttcaccga caccctcccg ccttcccgag ctccttcttc gctaatatca gctgcaggtg ttttatccgg caatgtggag ccgagcgtag ctggtagtgg cccttccccg cagtccgtaa cgacaataaa acgtgatgga tagccagcac tgtcgccaaa gggagcaacc tcatgcatga actccttcag gccctgcacc actcggccaa agcaaacgtg acgaccgttg agcgccttct catttgagtc agacgtggta atgaaaaatt gcgagccgtt gctgttgggt ccgttgttgg ccatcgccaa gagcccgact tcgttcatcc gccgtcgctt atactcatcc gcgaaccaac cgcgaccaaa gctactcaaa tgattcgcgc cattgtctac ccgcatggtg aggtccccgc cttgagcgca gaattgtgga ataatcttgt gaaagtatgt cccgcggtag gaaggcacga caccctggaa ccagtatgtc tcacccttcc ggctggttgt gcgccctgcg cacaaattcc tgaagttcgc acaagtcttg ggcgattcat catcaaaaag ttcaaattcc acgcgaaaga caggccccgg aggccccgcg gatttgacag agtccagcaa attatgacgt tgctgaacta aagacccgga gagggatggg gtttctggga aaccgttaaa acatgcgagc tccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagctcgca tgttttaacg gtttcccaga aaccccatcc ctctccgggt ctttagttca gcaacgtcat aatttgctgg actctgtcaa atccgcgggg cctccggggc ctgtctttcg cgtggaattt gaactttttg atgatgaatc gcccaagact tgtgcgaact tcaggaattt gtgcgcaggg cgcacaacca gccggaaggg tgagacatac tggttccagg gtgtcgtgcc ttcctaccgc gggacatact ttcacaagat tattccacaa ttctgcgctc aaggcgggga cctcaccatg cgggtagaca atggcgcgaa tcatttgagt agcttcggtc gcggttggtt cgcggatgag tataagcgac ggcggatgaa cgaagtcggg ctcttggcga tggccaacaa cggacccaac agcaacggct cgcaattttt cataaccacg tctgactcaa atgagaaggc gctcaacggt cgtcacgttt gctttggccg agtggtgcag ggcctgaagg agttcatgca tgaggttgct ccctttggcg acagtgctgg ctatccatca cgttttattg tcgttacgga ctgcggggaa gggccactac cagctacgct cggctccaca ttgccggata aaacacctgc agctgatatt agcgaagaag gagctcggga atgcgggagg gtgtcggtga agtccccttc cggcgggtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa