TrbrA.01252.a

protein kinase A catalytic subunit

CENTER ID: TrbrA.01252.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.01252.a.B1.GE40501 full length 1 334
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb09.211.2410
RefSeq: XP_827385.1
UniProt: Q38DR5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTTPTGDGQ LFTKPDTSGW KLSDFEMGDT LGTGSFGRVR IAKLKSRGEY YAIKCLKKHE ILKMKQVQHL NQEKQILMEL SHPFIVNMMC SFQDENRVYF VLEFVVGGEV FTHLRSAGRF PNDVAKFYHA ELVLAFEYLH SKDIIYRDLK PENLLLDGKG HVKVTDFGFA KKVTDRTYTL CGTPEYLAPE VIQSKGHGKA VDWWTMGVLL YEFIAGHPPF FDETPIRTYE KILAGRLKFP NWFDERARDL VKGLLQTDHT KRLGTLKDGV ADVKNHPFFR GANWEKLYGR HYNAPIAVKV KSPGDTSNFE SYPESGDKGS PPLTPSQQVA FRGF
NT Sequence
atgacgacaa ctcccactgg tgatggccaa ctgtttacca agcctgacac atcgggatgg aagctgagtg actttgaaat gggtgacacg ctagggaccg gctcgtttgg tcgcgtgcgc attgcaaaac tgaagagcag gggggagtat tatgcaataa aatgtctaaa gaagcatgag atactaaaga tgaagcaggt acaacacctg aaccaagaga agcaaattct aatggagttg tcacacccct tcattgtgaa catgatgtgt tccttccagg atgagaaccg cgtctacttt gttctagaat ttgtggtagg tggtgaggta tttactcacc ttcgttccgc aggccgtttc ccgaatgacg tagcgaagtt ctatcatgcg gagcttgtgt tggcctttga atatttacac tcgaaggaca ttatctaccg tgacttgaaa cctgagaatc tgctacttga tgggaaggga cacgtcaagg tgactgattt tggttttgct aagaaggtga cggatcgtac ctatacgtta tgtgggacac ctgagtatct tgcacctgag gtaattcaga gcaaaggaca tgggaaggct gtggattggt ggacgatggg tgttttgctg tatgaattca tagctggcca tcctcccttt tttgatgaaa ccccaattcg gacgtatgaa aagattcttg cgggccggct taaattcccc aattggtttg atgagcgtgc gcgggatctc gtaaagggtt tattgcaaac ggatcacacg aaacggttgg gcacgctgaa ggatggcgta gctgatgtga agaatcaccc attcttccgt ggtgcgaatt gggagaaact ctatggacgt cattataacg cccccattgc cgtaaaagtg aagagccccg gcgacacaag taactttgag tcgtatcccg agagtggaga taagggttct cctccactaa ccccttcgca acaggttgca ttccgtggtt tttag
Details for TrbrA.01252.a.B1.GE40501
HARVESTED ON: 8/10/2016
SEQUENCED ON: 8/11/2016
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 91
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMT TTPTGDGQLF TKPDTSGWKL SDFEMGDTLG TGSFGRVRIA KLKSRGEYYA IKCLKKHEIL KMKQVQHLNQ EKQILMELSH PFIVNMMCSF QDENRVYFVL EFVVGGEVFT HLRSAGRFPN DVAKFYHAEL VLAFEYLHSK DIIYRDLKPE NLLLDGKGHV KVTDFGFAKK VTDRTYTLCG TPEYLAPEVI QSKGHGKAVD WWTMGVLLYE FIAGHPPFFD ETPIRTYEKI LAGRLKFPNW FDERARDLVK GLLQTDHTKR LGTLXXXVXD VXNHPFXXGA XXEKLYGRHX XXPXXXXXXA XXXXXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatgacg acaactccca ctggtgatgg ccaactgttt accaagcctg acacatcggg atggaagctg agtgactttg aaatgggtga cacgctaggg accggctcgt ttggtcgcgt gcgcattgca aaactgaaga gcagggggga gtattatgca ataaaatgtc taaagaagca tgagatacta aagatgaagc aggtacaaca cctgaaccaa gagaagcaaa ttctaatgga gttgtcacac cccttcattg tgaacatgat gtgttccttc caggatgaga accgcgtcta ctttgttcta gaatttgtgg taggtggtga ggtatttact caccttcgtt ccgcaggccg tttcccgaat gacgtagcga agttctatca tgcggagctt gtgttggctt ttgaatattt acactcgaag gacattatct accgtgactt gaaacctgag aatctgctac ttgatgggaa gggacacgtc aaggtgactg attttggttt tgctaagaag gtgacggatc gtacctatac gttatgtggg acacctgagt atcttgcacc tgaggtaatt cagagcaaag gacatgggaa ggctgtggat tggtggacga tgggtgtttt gctgtatgaa ttcatagctg gccatcctcc cttttttgat gaaaccccaa ttcggacgta tgaaaagatt cttgcgggcc ggcttaaatt ccccaattgg tttgatgagc gtgcgcggga tctcgtaaag ggtttattgc aaacggatca cacgaaacgg ttgggcacgc tgannnatgn cgtanctgat gtgaanaatc acccattcnt ccnnggngcg aanngggaga aactctatgg acgtcatnat ancnccccnn ttnncntnaa ntgnanagcc nngnnnnnnn nnnnncnttn antnnta
Expected Protein Sequence
MAHHHHHHMT TTPTGDGQLF TKPDTSGWKL SDFEMGDTLG TGSFGRVRIA KLKSRGEYYA IKCLKKHEIL KMKQVQHLNQ EKQILMELSH PFIVNMMCSF QDENRVYFVL EFVVGGEVFT HLRSAGRFPN DVAKFYHAEL VLAFEYLHSK DIIYRDLKPE NLLLDGKGHV KVTDFGFAKK VTDRTYTLCG TPEYLAPEVI QSKGHGKAVD WWTMGVLLYE FIAGHPPFFD ETPIRTYEKI LAGRLKFPNW FDERARDLVK GLLQTDHTKR LGTLKDGVAD VKNHPFFRGA NWEKLYGRHY NAPIAVKVKS PGDTSNFESY PESGDKGSPP LTPSQQVAFR GF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga caactcccac tggtgatggc caactgttta ccaagcctga cacatcggga tggaagctga gtgactttga aatgggtgac acgctaggga ccggctcgtt tggtcgcgtg cgcattgcaa aactgaagag caggggggag tattatgcaa taaaatgtct aaagaagcat gagatactaa agatgaagca ggtacaacac ctgaaccaag agaagcaaat tctaatggag ttgtcacacc ccttcattgt gaacatgatg tgttccttcc aggatgagaa ccgcgtctac tttgttctag aatttgtggt aggtggtgag gtatttactc accttcgttc cgcaggccgt ttcccgaatg acgtagcgaa gttctatcat gcggagcttg tgttggcctt tgaatattta cactcgaagg acattatcta ccgtgacttg aaacctgaga atctgctact tgatgggaag ggacacgtca aggtgactga ttttggtttt gctaagaagg tgacggatcg tacctatacg ttatgtggga cacctgagta tcttgcacct gaggtaattc agagcaaagg acatgggaag gctgtggatt ggtggacgat gggtgttttg ctgtatgaat tcatagctgg ccatcctccc ttttttgatg aaaccccaat tcggacgtat gaaaagattc ttgcgggccg gcttaaattc cccaattggt ttgatgagcg tgcgcgggat ctcgtaaagg gtttattgca aacggatcac acgaaacggt tgggcacgct gaaggatggc gtagctgatg tgaagaatca cccattcttc cgtggtgcga attgggagaa actctatgga cgtcattata acgcccccat tgccgtaaaa gtgaagagcc ccggcgacac aagtaacttt gagtcgtatc ccgagagtgg agataagggt tctcctccac taaccccttc gcaacaggtt gcattccgtg gtttttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat