TrbrA.01191.a

Tb927.7.5480; Bifunctional dihydrofolate reductase-thymidylate synthase (TbDHFR-TS)

CENTER ID: TrbrA.01191.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.01191.a.A2.GE45586 soluble domain 1 432
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tb927.7.5480
UniProt: Q582G3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSLTRILRK KIPVHELAGK ISRPPLRPFS VVVASDEKGG IGDGGTIPWE IPEDMQYFRR VTTNLRGKNV KPSPSKRNAV VMGRKTWDSL PPKFRPLSNR LNVVLSRSAT KEQLLAGIPD PIKRAEAAND VVAVNGGLED ALRMLVSKEH TSSIETVFCI GGGTIYKQAL CAPCVNVLQA IHRTVVRPAS NSCSVFFDIP AAGTKTLEGL ELVRESITDE RVSTGAGGKK YQFEKLVPRN SEEEQYLNLV GRIIDEGCTK CDRTGVGTRS LFGAQMRFSL RNNRLPLLTT KRVFWRGVCE ELLWFLRGET NAKLLSDKGI HIWDGNGSRA FLDSRGLTDY DEMDLGPVYG FQWRHFGADY ISCKEDSEGK GVDQIANIVK SLIENPDDRR MICTAWNPAA LPRMALPPCH MMAQFYVSNG ELSCMLYQRS CDMGLGVPFN IASYALLTFL MAKASGLRPG ELVHTLGDAH VYSNHVEPCR KQLKRVPRPF PFIVFKQDKE FLEDFQESDI EVIDYSPYPV ISMEMAV
NT Sequence
atgctcagtc ttacgcgtat cctccgcaag aaaattcctg ttcatgagtt ggcggggaaa atttcccgcc ctcccctgcg acccttctct gttgttgtag cgtcggacga gaagggtggg attggggacg gtggtacgat cccctgggaa atacctgagg atatgcaata cttcagaagg gtaacgacaa accttcgggg gaaaaatgta aagccatcgc caagtaaacg gaatgctgtt gtgatggggc ggaaaacatg ggacagtctt ccaccaaagt tccgtccact gtctaaccgc cttaatgttg tgctgtcacg aagtgcgaca aaggaacagt tgttggctgg tatccctgat cccatcaaac gagcggaggc tgcgaatgat gtggttgctg tgaacggtgg gcttgaagac gcacttcgga tgttggtaag taaggagcac acatcttcta tcgaaacggt gttttgcatt ggtggcggga ctatatataa acaagccctt tgtgccccat gcgttaatgt gttgcaagct atccaccgga cggtggtccg accagcctca aattcgtgta gcgttttctt tgacatccca gccgcaggga caaagaccct ggaaggactt gagttggtgc gggaaagcat aacggacgaa cgggtatcaa ccggagctgg cggaaagaaa tatcagtttg agaagcttgt cccccgaaac agcgaagagg agcagtattt aaatctcgtt ggccgcataa ttgatgaggg atgtacgaaa tgtgaccgca cgggtgttgg tacccgctcc ctctttgggg cccagatgcg tttctctctc cgcaacaatc gcctgccatt actaaccacg aagcgtgtct tttggcgtgg agtatgtgaa gagctgttgt ggtttcttcg gggagaaaca aatgcaaagt tgctgagcga caaggggatt cacatatggg atgggaatgg ctcacgagca ttccttgaca gtcgtggctt gactgattat gatgagatgg acctcggacc tgtttatggc ttccagtgga ggcattttgg tgccgactat atttcttgca aggaggattc cgaggggaaa ggtgttgatc agattgcaaa cattgtgaag tcattgatag agaacccgga tgaccgtcgc atgatttgta cagcttggaa tccggccgcc ttgccacgca tggcgctccc accatgccac atgatggcgc aattctacgt gagcaatggg gaactctcat gcatgttgta ccagcggtca tgtgatatgg gtcttggcgt ccccttcaat attgcttcgt atgcactgct tacattcctc atggcaaaag cttctggact tcggccgggc gaattggtgc acacccttgg cgatgcacat gtttacagta accatgtgga gccgtgtcgg aagcaattga aacgcgtgcc ccgcccgttc cctttcatcg tgttcaaaca agacaaagag ttcctggaag actttcagga aagcgatata gaggtcatcg attattctcc gtaccccgta atttctatgg agatggcggt gtag
Details for TrbrA.01191.a.A2.GE45586
HARVESTED ON: data unavailable
SEQUENCED ON: 10/10/2024
EXPECTED MW: 50kDa
OBSERVED MW: data unavailable
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLSLTRILR KKIPVHELAG KISRPPLRPF SVVVASDEKG GIGDGGTIPW EIPEDMQYFR RVTTNLRGKN VKPSPSKRNA VVMGRKTWDS LPPKFRPLSN RLNVVLSRSA TKEQLLAGIP DPIKRAEAAN DVVAVNGGLE DALRMLVSKE HTSSIETVFC IGGGTIYKQA LCAPCVNVLQ AIHRTVVRPA SNSCSVFFDI PAAGTKTLEG LELVRESITD ERVSTGAGGK KYQFEKLVPR NSEEEQYLNL VGRIIDEGCT KCDRTGVGTR SLFGAQMRFS LRNNRLPLLT TKRVFWRGVC EELLWFLRGE TNAKLLSDKG IHIWDGNGSR AFLDSRGLTD YDEMDLGPVY GFQWRHFGAD YISCKVDSEG KGVDQIANIV KSLIENPDDR RMICTAWNPA ALPRMALPPC HMMAQFYVSN GELSCMLYQR SCD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctca gtcttacgcg tatcctccgc aagaaaattc ctgttcatga gttggcgggg aaaatttccc gccctcccct gcgacccttc tctgttgttg tagcgtcgga cgagaagggt gggattgggg acggtggtac gatcccctgg gaaatacctg aggacatgca atacttcaga agggtaacga caaaccttcg ggggaaaaat gtaaagccat cgccaagtaa acggaatgct gttgtgatgg ggcggaaaac atgggacagt cttccaccaa agttccgtcc actgtctaac cgccttaatg ttgtgctgtc acgaagtgcg acaaaggaac agttgttggc tggtatccct gatcccatca aacgagcgga ggctgcgaat gatgtggttg ccgtgaacgg tgggcttgaa gacgcacttc ggatgttggt aagtaaggag cacacatctt ctatcgaaac ggtgttttgc attggtggcg ggactatata taaacaagcc ctttgtgccc catgcgttaa tgtgttgcaa gctatccacc ggacggtggt ccgaccagcc tcaaattcgt gtagcgtttt ctttgacatc ccagccgcag ggacaaagac cctggaagga cttgagttgg tgcgggaaag cataacggac gaacgggtat caaccggagc tggcggaaag aaatatcagt ttgagaagct tgtcccccga aacagcgaag aggagcagta tttaaatctc gttggccgca taattgatga gggatgtacg aaatgtgacc gcacgggtgt tggtacccgc tccctctttg gggcccagat gcgtttctct ctccgcaaca atcgcctgcc attactaacc acgaagcgtg tcttttggcg tggagtatgt gaagagctgt tgtggtttct tcggggagaa acaaatgcaa agttgctgag cgacaagggg attcacatat gggatgggaa tggctcacga gcattccttg acagtcgtgg cttgactgat tatgatgaga tggacctcgg acctgtttat ggcttccagt ggaggcattt tggtgccgac tacatttctt gcaaagtgga ttccgagggg aagggtgttg atcagattgc aaacattgtg aagtcattga tagagaatcc ggatgaccgt cgcatgattt gtacagcttg gaatccggcc gccttgccac gcatggcgct cccaccatgc cacatgatgg cgcaattcta cgtgagcaat ggggaactct catgcatgtt gtaccagcgg tcatgtgatt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSLTRILR KKIPVHELAG KISRPPLRPF SVVVASDEKG GIGDGGTIPW EIPEDMQYFR RVTTNLRGKN VKPSPSKRNA VVMGRKTWDS LPPKFRPLSN RLNVVLSRSA TKEQLLAGIP DPIKRAEAAN DVVAVNGGLE DALRMLVSKE HTSSIETVFC IGGGTIYKQA LCAPCVNVLQ AIHRTVVRPA SNSCSVFFDI PAAGTKTLEG LELVRESITD ERVSTGAGGK KYQFEKLVPR NSEEEQYLNL VGRIIDEGCT KCDRTGVGTR SLFGAQMRFS LRNNRLPLLT TKRVFWRGVC EELLWFLRGE TNAKLLSDKG IHIWDGNGSR AFLDSRGLTD YDEMDLGPVY GFQWRHFGAD YISCKEDSEG KGVDQIANIV KSLIENPDDR RMICTAWNPA ALPRMALPPC HMMAQFYVSN GELSCMLYQR SCD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctcagtctt acgcgtatcc tccgcaagaa aattcctgtt catgagttgg cggggaaaat ttcccgccct cccctgcgac ccttctctgt tgttgtagcg tcggacgaga agggtgggat tggggacggt ggtacgatcc cctgggaaat acctgaggat atgcaatact tcagaagggt aacgacaaac cttcggggga aaaatgtaaa gccatcgcca agtaaacgga atgctgttgt gatggggcgg aaaacatggg acagtcttcc accaaagttc cgtccactgt ctaaccgcct taatgttgtg ctgtcacgaa gtgcgacaaa ggaacagttg ttggctggta tccctgatcc catcaaacga gcggaggctg cgaatgatgt ggttgctgtg aacggtgggc ttgaagacgc acttcggatg ttggtaagta aggagcacac atcttctatc gaaacggtgt tttgcattgg tggcgggact atatataaac aagccctttg tgccccatgc gttaatgtgt tgcaagctat ccaccggacg gtggtccgac cagcctcaaa ttcgtgtagc gttttctttg acatcccagc cgcagggaca aagaccctgg aaggacttga gttggtgcgg gaaagcataa cggacgaacg ggtatcaacc ggagctggcg gaaagaaata tcagtttgag aagcttgtcc cccgaaacag cgaagaggag cagtatttaa atctcgttgg ccgcataatt gatgagggat gtacgaaatg tgaccgcacg ggtgttggta cccgctccct ctttggggcc cagatgcgtt tctctctccg caacaatcgc ctgccattac taaccacgaa gcgtgtcttt tggcgtggag tatgtgaaga gctgttgtgg tttcttcggg gagaaacaaa tgcaaagttg ctgagcgaca aggggattca catatgggat gggaatggct cacgagcatt ccttgacagt cgtggcttga ctgattatga tgagatggac ctcggacctg tttatggctt ccagtggagg cattttggtg ccgactatat ttcttgcaag gaggattccg aggggaaagg tgttgatcag attgcaaaca ttgtgaagtc attgatagag aacccggatg accgtcgcat gatttgtaca gcttggaatc cggccgcctt gccacgcatg gcgctcccac catgccacat gatggcgcaa ttctacgtga gcaatgggga actctcatgc atgttgtacc agcggtcatg tgattaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa