SSGCID: Target Page

TrbrA.00934.a

Methylene-tetrahydrofolate dehydrogenase/cyclohydrolase (DHCH1)

CENTER ID:	TrbrA.00934.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00934.a.B1.GE35065	full length		1	298

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00934.a.B1.PW35682	full length		1	298

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.7.1600
RefSeq:	XP_845784.1
UniProt:	Q57WP0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPEAVVIDGR AVAKAIQKEL TEEVALLERR YKGRRPGLST IICGKRKDSQ TYVRLKRKAA AACGFRNFSV ELPANVTQET LEREVIRLNE EEACHSIVVQ LPLPPHIDKV AALSKIKPEK DADCLLPVNV GQLHIRERNP AIVPCTASAV MELLRCSGVE ICGKRVVVLG RGDIAGLPVA TLLANEDATV TVVHSATPLC DIADIVRASD IVVSAAGQPG LVRGEWIKLG AAVIDVGTTP VADPSKVPGY RLVGDVCFDV ARKRAAYITP VPGGVGPVTV SMLLKNTLTM FKRSVRAL

NT Sequence

atgcctgagg cggttgtaat tgacggcaga gcggtggcga aggccattca aaaggaactg actgaagagg tcgcattgtt ggaacgccgt tacaaaggaa gacgtccggg gctctccacc atcatttgcg gtaagcgtaa ggactctcaa acgtatgtcc gcttgaaacg taaggccgct gccgcttgcg ggtttcgcaa ctttagtgtg gaactccctg caaatgttac gcaggaaact ttggaacggg aggtgattag gttaaacgag gaggaagcat gccatagcat cgttgtgcag ttaccgcttc cgccgcacat cgacaaggtg gcagcgttat cgaagattaa gccggagaag gatgcagact gtttacttcc agtcaatgtg ggtcagctcc acattaggga acgcaatcct gccattgttc cgtgcaccgc atctgccgtg atggagttgc ttaggtgcag tggcgtggaa atatgtggaa agcgggtagt ggtgcttgga cggggtgaca tcgctggtct gcccgttgcc actttgctgg caaatgaaga tgccacggtt actgtagtgc actccgcgac gccactatgt gacattgcgg acattgttcg agcgtccgat atagttgtgt ctgctgcagg tcagccgggc cttgttcgcg gggagtggat caagcttggt gctgcagtta ttgacgtggg caccacaccc gtcgccgatc catcgaaggt gccgggatat cgtcttgtgg gtgacgtttg ctttgatgtg gcgcgtaagc gagccgctta tatcacacct gtgcctggcg gtgttggtcc tgtgactgtc agcatgctgc tgaagaacac tcttacgatg tttaaaagaa gcgttcgtgc cttgtga

Details for TrbrA.00934.a.B1.GE35065

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	32kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP EAVVIDGRAV AKAIQKELTE EVALLERRYK GRRPGLSTII CGKRKDSQTY VRLKRKAAAA CGFRNFSVEL PANVTQEALE REVIRLNEEE ACHSIVVQLP LPPHIDKVAA LSKIKPEKDA DCLLPVNVGQ LHIRERNPAI VPCTASAVME LLKCSGVEIC GKRVVVLGRG DIAGLPVATL LANEDATVTV VHSATPLCDI ADIVRASDIV VSAAGQPGLV RGEWIKLGAA VIDVGTTPVA DPSKVPGYRL VGDVCFDVAR KRAAYITPVP GGVGPVTVSM LLKNTLTMFK RSVRAL

Validated NT Sequence

tactcactta caaggcacga acgcttcttt taaacatcgt aagagtgttc ttcagcagca tgctgacagt cacaggacca acaccgccag gcacaggtgt gatataagcg gctcgcttac gcgccacatc aaagcaaacg tcacccacaa gacgatatcc cggcaccttc gatggatcgg cgacgggtgt ggtgcccacg tcaataactg cagcaccaag cttgatccac tccccgcgaa caaggcccgg ctgacctgca gcagacacaa ctatatcgga cgctcgaaca atgtccgcaa tgtcacatag tggcgtcgcg gagtgcacta cagtaaccgt ggcatcttca tttgccagca aagttgcaac gggcagacca gcgatgtcac cccgtccaag caccactacc cgctttccac atatttccac gccactgcac ttaagcaact ccatgacggc agatgcggtg cacggaacaa tggcaggatt gcgttcccta atgtgtagct gacccacatt gactggaagt aaacagtctg catccttctc cggcttaatc ttcgataacg ctgccacctt gtcgatgtgc ggcggaagcg gtaactgcac aacgatgcta tggcatgctt cctcctcgtt taacctaatc acctcccgtt ccaaagcttc ctgcgtaaca tttgcaggga gttccacact aaagttgcga aacccgcaag cggcagcggc cttacgtttc aagcggacat acgtttgaga gtccttacgc ttaccgcaaa tgatggtgga gagccccgga cgtcttcctt tgtaacggcg ttccaacaat gcgacctctt cagtcagttc cttttgaatg gccttcgcca ccgctctgcc gtcaattaca accgcctcag gcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHMP EAVVIDGRAV AKAIQKELTE EVALLERRYK GRRPGLSTII CGKRKDSQTY VRLKRKAAAA CGFRNFSVEL PANVTQETLE REVIRLNEEE ACHSIVVQLP LPPHIDKVAA LSKIKPEKDA DCLLPVNVGQ LHIRERNPAI VPCTASAVME LLRCSGVEIC GKRVVVLGRG DIAGLPVATL LANEDATVTV VHSATPLCDI ADIVRASDIV VSAAGQPGLV RGEWIKLGAA VIDVGTTPVA DPSKVPGYRL VGDVCFDVAR KRAAYITPVP GGVGPVTVSM LLKNTLTMFK RSVRAL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctg aggcggttgt aattgacggc agagcggtgg cgaaggccat tcaaaaggaa ctgactgaag aggtcgcatt gttggaacgc cgttacaaag gaagacgtcc ggggctctcc accatcattt gcggtaagcg taaggactct caaacgtatg tccgcttgaa acgtaaggcc gctgccgctt gcgggtttcg caactttagt gtggaactcc ctgcaaatgt tacgcaggaa actttggaac gggaggtgat taggttaaac gaggaggaag catgccatag catcgttgtg cagttaccgc ttccgccgca catcgacaag gtggcagcgt tatcgaagat taagccggag aaggatgcag actgtttact tccagtcaat gtgggtcagc tccacattag ggaacgcaat cctgccattg ttccgtgcac cgcatctgcc gtgatggagt tgcttaggtg cagtggcgtg gaaatatgtg gaaagcgggt agtggtgctt ggacggggtg acatcgctgg tctgcccgtt gccactttgc tggcaaatga agatgccacg gttactgtag tgcactccgc gacgccacta tgtgacattg cggacattgt tcgagcgtcc gatatagttg tgtctgctgc aggtcagccg ggccttgttc gcggggagtg gatcaagctt ggtgctgcag ttattgacgt gggcaccaca cccgtcgccg atccatcgaa ggtgccggga tatcgtcttg tgggtgacgt ttgctttgat gtggcgcgta agcgagccgc ttatatcaca cctgtgcctg gcggtgttgg tcctgtgact gtcagcatgc tgctgaagaa cactcttacg atgtttaaaa gaagcgttcg tgccttgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for TrbrA.00934.a.B1.PW35682

PURIFICATION DATe:	6/1/2012
CONCENTRATION:	28.28mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTG AGGCGGTTGT AATTGACGGC AGAGCGGTGG CGAAGGCCAT TCAAAAGGAA CTGACTGAAG AGGTCGCATT GTTGGAACGC CGTTACAAAG GAAGACGTCC GGGGCTCTCC ACCATCATTT GCGGTAAGCG TAAGGACTCT CAAACGTATG TCCGCTTGAA ACGTAAGGCC GCTGCCGCTT GCGGGTTTCG CAACTTTAGT GTGGAACTCC CTGCAAATGT TACGCAGGAA ACTTTGGAAC GGGAGGTGAT TAGGTTAAAC GAGGAGGAAG CATGCCATAG CATCGTTGTG CAGTTACCGC TTCCGCCGCA CATCGACAAG GTGGCAGCGT TATCGAAGAT TAAGCCGGAG AAGGATGCAG ACTGTTTACT TCCAGTCAAT GTGGGTCAGC TCCACATTAG GGAACGCAAT CCTGCCATTG TTCCGTGCAC CGCATCTGCC GTGATGGAGT TGCTTAGGTG CAGTGGCGTG GAAATATGTG GAAAGCGGGT AGTGGTGCTT GGACGGGGTG ACATCGCTGG TCTGCCCGTT GCCACTTTGC TGGCAAATGA AGATGCCACG GTTACTGTAG TGCACTCCGC GACGCCACTA TGTGACATTG CGGACATTGT TCGAGCGTCC GATATAGTTG TGTCTGCTGC AGGTCAGCCG GGCCTTGTTC GCGGGGAGTG GATCAAGCTT GGTGCTGCAG TTATTGACGT GGGCACCACA CCCGTCGCCG ATCCATCGAA GGTGCCGGGA TATCGTCTTG TGGGTGACGT TTGCTTTGAT GTGGCGCGTA AGCGAGCCGC TTATATCACA CCTGTGCCTG GCGGTGTTGG TCCTGTGACT GTCAGCATGC TGCTGAAGAA CACTCTTACG ATGTTTAAAA GAAGCGTTCG TGCCTTGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT