TrbrA.00855.a
glyceraldehyde 3-phosphate dehydrogenase, glycosomal
| CENTER ID: | TrbrA.00855.a |
| ORGANISM: | Trypanosoma brucei TREU927 |
| ASSOCIATED DISEASE: | African trypanosomiasis (African Sleeping Sickness) |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrbrA.00855.a.B1.GE35079 | full length | 1 | 331 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrbrA.00855.a.B1.PS01548 | full length | 1 | 331 |
Structures
4P8R
DEPOSITED: 3/31/2014
DETERMINATION: XRay
CLONE: TrbrA.00855.a.B1.GE35079
PROTEIN: TrbrA.00855.a.B1.PS01548
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:Tb927.10.6880 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVIRVGINGF GRIGRVVFRA AQRRNDIEIV GINDLLDADY MAYMLKYDST HGRFEGAVEV QGGALVVNGK KIRVTSERDP ANLKWNEINV DVVVESTGLF LSDDTARKHI QAGAKKVVIT GPSKDDTPMF VMGVNHTTYK GEAIVSNASC TTNCLAPLAK VLNDKFGIVE GLMTTVHATT ATQKTVDGPS QKDWRGGRGA AQNIIPSSTG AAKAVGKIIP SLNGKLTGMA FRVPTPNVSV VDLTVRLERP ATYKQICDAI KAASEGELKG ILGYVDEEIV SSDINGIPLT SVFDARAGIS LNDNFVKLVS WYDNETGYSN KVLDLIAHIT K
NT Sequence
atggtaatca gagtcggcat caatgggttt ggtcgtattg gccgcgttgt gttccgagct gcgcagcgcc gaaacgacat cgaaattgtt gggatcaacg atttgctcga tgcggattac atggcttaca tgttgaagta tgattccact cacggccgct tcgaaggtgc ggtagaggtt caaggtggtg cacttgtggt aaacggcaag aagattcgcg tcacgtccga gcgggatccc gcgaacttga agtggaatga aattaatgtg gacgttgttg tggagtctac tggacttttt ctctccgacg atacggcacg aaagcacatc caggctggtg ccaagaaggt cgtcatcaca ggtccttcga aggacgacac accaatgttc gttatgggtg taaaccacac gacttacaag ggggaagcca tcgtctcaaa cgcatcatgc acgacgaact gccttgcccc ccttgcaaag gtgctcaacg acaagtttgg catcgtggaa ggactcatga caaccgttca cgcaacaacg gcgacacaga aaactgtcga tggcccctcg cagaaggact ggcgtggcgg caggggtgca gcgcagaata ttattccttc atccaccggt gcggcgaaag cggtgggcaa aatcatcccg tcgctcaatg gcaaactcac tggtatggcc ttccgtgtac caactcccaa cgtttccgtg gtggatctca cagtgcggct agagcgtccc gcgacctaca agcaaatttg cgacgccatt aaggctgcat cagagggtga actgaagggg attttgggtt atgtggacga agagattgtt tccagtgaca tcaacggcat tccgctcaca tccgtcttcg atgccagggc gggtatttcg ctgaacgaca acttcgttaa gctggtctcg tggtatgaca acgaaaccgg gtactcaaac aaggtcctag acctcattgc gcacatcaca aagtga
Details for TrbrA.00855.a.B1.GE35079
| HARVESTED ON: | 4/10/2012 |
| SEQUENCED ON: | 4/11/2012 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MVIRVGINGF GRIGRVVFRA AQRRNDIEIV GINDLLDADY MAYMLKYDST HGRFEGAVEV QGGALVVNGK KIRVTSERDP ANLKWNEINV DVVVESTGLF LSDDTARKHI QAGAKKVVIT GPSKDDTPMF VMGVNHTTYK GEAIVSNASC TTNCLAPLAK VLNDKFGIVE GLMTTVHATT ATQKTVDGPS QKDWRGGRGA AQNIIPSSTG AAKAVGKIIP SLNGKLTGMA FRVPTPNVSV VDLTVRLERP ATYKQICDAI KAASEGELKG ILGYVDEEIV SSDINGIPLT SVFDARAGIS LNDNFVKLVS WYDNETGYSN KVLDLIAHIT K
Validated NT Sequence
tactcactta ctttgtgatg tgcgcaatga ggtctaggac cttgtttgag tacccggttt cgttgtcata ccacgagacc agcttaacga agttgtcgtt cagcgaaata cccgccctgg catcgaagac ggatgtgagc ggaatgccgt tgatgtcact ggaaacaatc tcttcgtcca cataacccaa aatccccttc agttcaccct ctgatgcagc cttaatggcg tcgcaaattt gcttgtaggt cgcgggacgc tctagccgca ctgtgagatc caccacggaa acgttgggag ttggtacacg gaaggccata ccagtgagtt tgccattgag cgacgggatg attttgccca ccgctttcgc cgcaccggtg gatgaaggaa taatattctg cgccgcaccc ctgccgccac gccagtcctt ctgcgagggg ccatcgacag ttttctgtgt cgccgttgtt gcgtgaacgg ttgtcatgag tccttccacg atgccaaact tgtcgttgag cacctttgca aggggggcaa ggcagttcgt cgtgcatgat gcgtttgaga cgatggcttc ccccttgtaa gtcgtgtggt ttacacccat aacgaacatt ggtgtgtcgt ccttcgaagg acctgtgatg acgaccttct tggcaccagc ctggatgtgc tttcgtgccg tatcgtcgga gagaaaaagt ccagtagact ccacaacaac gtccacatta atttcattcc acttcaagtt cgcgggatcc cgctcggacg tgacgcgaat cttcttgcca tttaccacaa gtgcaccacc ttgaacctct accgcacctt cgaagcggcc gtgagtggaa tcatacttca acatgtaagc catgtaatcc gcatcgagca aatcgttgat cccaacaatt tcaatgtcgt ttcggcgctg cgcagctcgg aacacaacgc ggccaatacg gccaaaccca ttgatgccga ctctgattac catatggtgg
Expected Protein Sequence
MAHHHHHHMV IRVGINGFGR IGRVVFRAAQ RRNDIEIVGI NDLLDADYMA YMLKYDSTHG RFEGAVEVQG GALVVNGKKI RVTSERDPAN LKWNEINVDV VVESTGLFLS DDTARKHIQA GAKKVVITGP SKDDTPMFVM GVNHTTYKGE AIVSNASCTT NCLAPLAKVL NDKFGIVEGL MTTVHATTAT QKTVDGPSQK DWRGGRGAAQ NIIPSSTGAA KAVGKIIPSL NGKLTGMAFR VPTPNVSVVD LTVRLERPAT YKQICDAIKA ASEGELKGIL GYVDEEIVSS DINGIPLTSV FDARAGISLN DNFVKLVSWY DNETGYSNKV LDLIAHITK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtaa tcagagtcgg catcaatggg tttggtcgta ttggccgcgt tgtgttccga gctgcgcagc gccgaaacga catcgaaatt gttgggatca acgatttgct cgatgcggat tacatggctt acatgttgaa gtatgattcc actcacggcc gcttcgaagg tgcggtagag gttcaaggtg gtgcacttgt ggtaaacggc aagaagattc gcgtcacgtc cgagcgggat cccgcgaact tgaagtggaa tgaaattaat gtggacgttg ttgtggagtc tactggactt tttctctccg acgatacggc acgaaagcac atccaggctg gtgccaagaa ggtcgtcatc acaggtcctt cgaaggacga cacaccaatg ttcgttatgg gtgtaaacca cacgacttac aagggggaag ccatcgtctc aaacgcatca tgcacgacga actgccttgc cccccttgca aaggtgctca acgacaagtt tggcatcgtg gaaggactca tgacaaccgt tcacgcaaca acggcgacac agaaaactgt cgatggcccc tcgcagaagg actggcgtgg cggcaggggt gcagcgcaga atattattcc ttcatccacc ggtgcggcga aagcggtggg caaaatcatc ccgtcgctca atggcaaact cactggtatg gccttccgtg taccaactcc caacgtttcc gtggtggatc tcacagtgcg gctagagcgt cccgcgacct acaagcaaat ttgcgacgcc attaaggctg catcagaggg tgaactgaag gggattttgg gttatgtgga cgaagagatt gtttccagtg acatcaacgg cattccgctc acatccgtct tcgatgccag ggcgggtatt tcgctgaacg acaacttcgt taagctggtc tcgtggtatg acaacgaaac cgggtactca aacaaggtcc tagacctcat tgcgcacatc acaaagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for TrbrA.00855.a.B1.PS01548
| PURIFICATION DATe: | 7/3/2012 |
| CONCENTRATION: | 77.6mg/ml |
| OBSERVED MW: | 33kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MVIRVGINGF GRIGRVVFRA AQRRNDIEIV GINDLLDADY MAYMLKYDST HGRFEGAVEV QGGALVVNGK KIRVTSERDP ANLKWNEINV DVVVESTGLF LSDDTARKHI QAGAKKVVIT GPSKDDTPMF VMGVNHTTYK GEAIVSNASC TTNCLAPLAK VLNDKFGIVE GLMTTVHATT ATQKTVDGPS QKDWRGGRGA AQNIIPSSTG AAKAVGKIIP SLNGKLTGMA FRVPTPNVSV VDLTVRLERP ATYKQICDAI KAASEGELKG ILGYVDEEIV SSDINGIPLT SVFDARAGIS LNDNFVKLVS WYDNETGYSN KVLDLIAHIT K
Validated NT Sequence
tactcactta ctttgtgatg tgcgcaatga ggtctaggac cttgtttgag tacccggttt cgttgtcata ccacgagacc agcttaacga agttgtcgtt cagcgaaata cccgccctgg catcgaagac ggatgtgagc ggaatgccgt tgatgtcact ggaaacaatc tcttcgtcca cataacccaa aatccccttc agttcaccct ctgatgcagc cttaatggcg tcgcaaattt gcttgtaggt cgcgggacgc tctagccgca ctgtgagatc caccacggaa acgttgggag ttggtacacg gaaggccata ccagtgagtt tgccattgag cgacgggatg attttgccca ccgctttcgc cgcaccggtg gatgaaggaa taatattctg cgccgcaccc ctgccgccac gccagtcctt ctgcgagggg ccatcgacag ttttctgtgt cgccgttgtt gcgtgaacgg ttgtcatgag tccttccacg atgccaaact tgtcgttgag cacctttgca aggggggcaa ggcagttcgt cgtgcatgat gcgtttgaga cgatggcttc ccccttgtaa gtcgtgtggt ttacacccat aacgaacatt ggtgtgtcgt ccttcgaagg acctgtgatg acgaccttct tggcaccagc ctggatgtgc tttcgtgccg tatcgtcgga gagaaaaagt ccagtagact ccacaacaac gtccacatta atttcattcc acttcaagtt cgcgggatcc cgctcggacg tgacgcgaat cttcttgcca tttaccacaa gtgcaccacc ttgaacctct accgcacctt cgaagcggcc gtgagtggaa tcatacttca acatgtaagc catgtaatcc gcatcgagca aatcgttgat cccaacaatt tcaatgtcgt ttcggcgctg cgcagctcgg aacacaacgc ggccaatacg gccaaaccca ttgatgccga ctctgattac catatggtgg
Expressed Protein Sequence
MAHHHHHHMV IRVGINGFGR IGRVVFRAAQ RRNDIEIVGI NDLLDADYMA YMLKYDSTHG RFEGAVEVQG GALVVNGKKI RVTSERDPAN LKWNEINVDV VVESTGLFLS DDTARKHIQA GAKKVVITGP SKDDTPMFVM GVNHTTYKGE AIVSNASCTT NCLAPLAKVL NDKFGIVEGL MTTVHATTAT QKTVDGPSQK DWRGGRGAAQ NIIPSSTGAA KAVGKIIPSL NGKLTGMAFR VPTPNVSVVD LTVRLERPAT YKQICDAIKA ASEGELKGIL GYVDEEIVSS DINGIPLTSV FDARAGISLN DNFVKLVSWY DNETGYSNKV LDLIAHITK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTAA TCAGAGTCGG CATCAATGGG TTTGGTCGTA TTGGCCGCGT TGTGTTCCGA GCTGCGCAGC GCCGAAACGA CATCGAAATT GTTGGGATCA ACGATTTGCT CGATGCGGAT TACATGGCTT ACATGTTGAA GTATGATTCC ACTCACGGCC GCTTCGAAGG TGCGGTAGAG GTTCAAGGTG GTGCACTTGT GGTAAACGGC AAGAAGATTC GCGTCACGTC CGAGCGGGAT CCCGCGAACT TGAAGTGGAA TGAAATTAAT GTGGACGTTG TTGTGGAGTC TACTGGACTT TTTCTCTCCG ACGATACGGC ACGAAAGCAC ATCCAGGCTG GTGCCAAGAA GGTCGTCATC ACAGGTCCTT CGAAGGACGA CACACCAATG TTCGTTATGG GTGTAAACCA CACGACTTAC AAGGGGGAAG CCATCGTCTC AAACGCATCA TGCACGACGA ACTGCCTTGC CCCCCTTGCA AAGGTGCTCA ACGACAAGTT TGGCATCGTG GAAGGACTCA TGACAACCGT TCACGCAACA ACGGCGACAC AGAAAACTGT CGATGGCCCC TCGCAGAAGG ACTGGCGTGG CGGCAGGGGT GCAGCGCAGA ATATTATTCC TTCATCCACC GGTGCGGCGA AAGCGGTGGG CAAAATCATC CCGTCGCTCA ATGGCAAACT CACTGGTATG GCCTTCCGTG TACCAACTCC CAACGTTTCC GTGGTGGATC TCACAGTGCG GCTAGAGCGT CCCGCGACCT ACAAGCAAAT TTGCGACGCC ATTAAGGCTG CATCAGAGGG TGAACTGAAG GGGATTTTGG GTTATGTGGA CGAAGAGATT GTTTCCAGTG ACATCAACGG CATTCCGCTC ACATCCGTCT TCGATGCCAG GGCGGGTATT TCGCTGAACG ACAACTTCGT TAAGCTGGTC TCGTGGTATG ACAACGAAAC CGGGTACTCA AACAAGGTCC TAGACCTCAT TGCGCACATC ACAAAGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T