TrbrA.00825.a

aspartyl-tRNA synthetase, putative

CENTER ID: TrbrA.00825.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.00825.a.B1.GE42775 full length 1 541
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tb927.10.1260
UniProt: Q38CB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYRFWPSFSP FPTLKLLNIH RWCCSATGKP PMVGVAAIQR GALQSAASAR VRGRVENFRV RGKLAFIQLR QPPSHSIQVV AAGAEISHKV KELTPESIVD VIGVIKPTER PVTSVSCSNY ELHATSIEVV SKAAVPLPFP LHDQNAKLDT RLNNRYIDMR TPLAVAMIRL VSAAAQSFRT QLLSRDFVEL HTPKIIGTAS EGGSALFSLD YFGRQAFLAQ SPQLYKQMAI MGDGMRVFEI GPVFRAEKSL THRHLTEFTG LDAEFTVDEF YTELLDVLES ILCGMIWKLQ TENAEWISQA RAALMELDST EDCGTVVPRR EDIVCEVAEE IIAMYNIRFD DAASRIAPSV PTEPSEDRYH ARIGTSGPKV LRMTFRDALQ LIRDNDHQHA GRQQEVVGES DEEVVVDFNL NQERALGELI RLRYGVDLYV VDQFPLAARP FYTMPHPSEE GVACGFDMYL RGEEICSGGQ RIHDVAQLIH SMEQRRMEAA HMRDYVDSFR YGAWPHGGFG LGLERIVLFL LGARDIRRVS LFPRDPRRLA P
NT Sequence
atgtatcgtt tctggccgag cttctcgccg tttccaactt tgaaactcct caacatccat cgctggtgtt gcagcgctac aggaaagcca cccatggtgg gtgttgctgc catacagcgc ggcgcgttac aatcagcggc atcggcaagg gttcgcggtc gtgtggagaa ttttcgtgtc cgcggcaagc tggctttcat tcaactccgc caaccaccat cgcactccat tcaagttgta gctgctgggg ctgaaatctc acacaaggtg aaggaactta cgcctgagtc tattgttgat gttattggtg taatcaaacc tactgagcga cccgtgacat ccgtcagttg ctcgaactat gaactgcatg cgacatctat agaagttgtg agcaaggcag ctgtaccgct tccttttccg cttcacgacc agaacgcgaa gctggacacg cgcctgaaca accgatacat cgacatgcga acaccgttgg cggttgctat gatacgtctg gtttcagccg ctgcgcagag cttccgcacg cagcttttgt cccgcgactt tgtggaactt cacacgccaa agatcatagg cacagcatca gagggtggta gtgcgctctt ctcactggat tactttggtc gtcaagcctt cttggcgcag tcgcctcagt tgtacaagca aatggcaata atgggtgatg gtatgcgagt gttcgaaatt gggcccgttt tccgcgcgga gaagagtctt acgcatcgtc atctcacgga gttcacagga ctggatgctg aatttacagt cgacgaattc tatactgagt tgttggatgt gctagagtca atattatgcg gcatgatttg gaaacttcag acagaaaacg ccgagtggat cagccaggca cgagcggcat taatggagtt ggacagtact gaggactgcg gcactgtggt gcctcgtcgt gaagatatag tatgcgaggt agcggaggag ataattgcca tgtacaatat acgttttgat gacgcggcga gccgaatagc accttccgtg ccgactgaac cctcggagga tcgctatcat gcgcgtatcg gtacaagcgg gccaaaggtg ctgcgtatga cattcaggga tgcactgcaa ctcatccggg ataacgatca tcaacacgct ggaaggcaac aagaggtcgt gggggaaagt gacgaagagg ttgtggtcga cttcaacctc aaccaggagc gggcactcgg ggagttgatt cggttacgct acggcgtcga tttgtacgtt gtggatcaat tcccactggc tgcacgaccc ttttacacga tgccacaccc gagtgaagag ggtgtagcct gtggcttcga tatgtatttg cgtggagagg aaatttgtag cggcgggcag cggatacacg acgtggcgca gctcattcat agcatggagc agcggcgaat ggaagcagca catatgaggg actacgtgga ctcattccgg tacggtgcgt ggccacacgg tggcttcggc ctcggacttg aacgtatcgt gctttttctc ttgggtgcgc gtgacatcag gcgtgtttca ctatttcccc gtgatccgag gcggttggcg ccatga
Details for TrbrA.00825.a.B1.GE42775
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 62kDa
OBSERVED MW: 62kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 77
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMY RFWPSFSPFP TLKLLNIHRW CCSATGKPPM VGVAAIQRGA LQSAASARVR GRVENFRVRG KLAFIQLRQP PSHSIQVVAA GAEISHRVKE LTPESIVDVI GEIKPTERPV TSVSCSNYEL HATSIEVVSK AAVPLPFPLH DQNAKLDTRL NNRYIDMRTP LAVAMXXLVS AAAXXFRTQL FVPRLCXTXH AXXXRHXXXG WXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXIYXXXXX XXXVGXXXXN IXXHDXXXQT ENXXXISQAR XXLMELDSAE DCGTVVPRRE DIVCEVAEEI IAMYNIRFDD AASRIAPSVP TEPSEDRYHA RIGTSGPKVL RMTFRDALQL IRDNDHQHAG RQQEVAGESD GEVVVDFNLN QERALGELIR LRYGVDLYVV DQFPLAARPF YTMPHPSEEG VACGFDMYLR GEEICSGGQR IHDVAQLIHS MEQRRMEAAH MRDYVDSFRY GAWPHGGFGL GLERIVLFLL GARDIRRVSL F
Validated NT Sequence
ggaaatagtg aaacacgcct gatgtcacgc gcacccaaga gaaaaagcac gatacgttca agtccgaggc cgaagccacc gtgtggccac gcaccgtacc ggaatgagtc cacgtagtcc ctcatatgtg ctgcttccat tcgccgctgc tccatgctat gaatgagctg cgccacgtcg tgtatccgct gcccgccgct acaaatttcc tctccacgca aatacatatc gaagccacag gctacaccct cttcactcgg gtgtggcatc gtgtaaaagg gtcgtgcagc cagtgggaat tgatccacaa cgtacaaatc gacgccgtag cgtaaccgaa tcaactcccc gagtgcccgc tcctggttga ggttgaagtc gaccacaacc tctccgtcac tttcccccgc gacctcttgt tgccttccag cgtgttgatg atcgttatcc cggatgagtt gcagtgcatc cctgaatgtc atacgcagca cctttggccc gcttgtaccg atacgcgcat gatagcgatc ctccgagggt tcagtcggca cggaaggtgc tattcggctc gccgcgtcat caaaacgtat attgtacatg gcaattatct cctccgctac ctcgcatact atatcttcac gacgaggcac cacagtgccg cagtcctcag cactgtccaa ctccattaat nnnnntcgtg cctggctgat cnnntnnncg ttttctgtct gnnnnnnnna atcatgccnn ataatattnn nnnnnnnnnn nccaacnnnn cannnnnnna nnnnnnnnnt gtaaatnnnn nannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt atnnnnnnnn nnnnnnnnnn nnnnnngnnn nnnnnnnnnn nnnnnnnnnn nacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccacccnn nnnnnctgtg cctatnancn nnggcgtgtn nngtnnnaca aagtcgcggg acaaaaagct gcgtgcggaa gnnnnncgca gcggcngaaa ccagacnnnt catagcaacc gccaacggtg ttcgcatgtc gatgtatcgg ttgttcaggc gcgtgtccag cttcgcgttc tggtcgtgaa gcggaaaagg aagcggtaca gctgccttgc tcacaacttc tatagatgtc gcatgcagtt catagttcga gcaactgacg gatgtcacgg gtcgctcagt aggtttgatt tcaccaataa catcaacaat agactcaggc gtaagttcct tcaccctgtg tgagatttca gccccagcag ctacaacttg aatggagtgc gatggtggtt ggcggagttg aatgaaagcc agcttgccgc ggacacgaaa attctccaca cgaccgcgaa cccttgccga tgccgctgat tgtaacgcgc cgcgctgtat ggcagcaaca cccaccatgg gtggctttcc tgtagcgctg caacaccagc gatggatgtt gaggagtttc aaagttggaa acggcgagaa gctcggccag aaacgataca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMY RFWPSFSPFP TLKLLNIHRW CCSATGKPPM VGVAAIQRGA LQSAASARVR GRVENFRVRG KLAFIQLRQP PSHSIQVVAA GAEISHKVKE LTPESIVDVI GVIKPTERPV TSVSCSNYEL HATSIEVVSK AAVPLPFPLH DQNAKLDTRL NNRYIDMRTP LAVAMIRLVS AAAQSFRTQL LSRDFVELHT PKIIGTASEG GSALFSLDYF GRQAFLAQSP QLYKQMAIMG DGMRVFEIGP VFRAEKSLTH RHLTEFTGLD AEFTVDEFYT ELLDVLESIL CGMIWKLQTE NAEWISQARA ALMELDSTED CGTVVPRRED IVCEVAEEII AMYNIRFDDA ASRIAPSVPT EPSEDRYHAR IGTSGPKVLR MTFRDALQLI RDNDHQHAGR QQEVVGESDE EVVVDFNLNQ ERALGELIRL RYGVDLYVVD QFPLAARPFY TMPHPSEEGV ACGFDMYLRG EEICSGGQRI HDVAQLIHSM EQRRMEAAHM RDYVDSFRYG AWPHGGFGLG LERIVLFLLG ARDIRRVSLF PRDPRRLAP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtatc gtttctggcc gagcttctcg ccgtttccaa ctttgaaact cctcaacatc catcgctggt gttgcagcgc tacaggaaag ccacccatgg tgggtgttgc tgccatacag cgcggcgcgt tacaatcagc ggcatcggca agggttcgcg gtcgtgtgga gaattttcgt gtccgcggca agctggcttt cattcaactc cgccaaccac catcgcactc cattcaagtt gtagctgctg gggctgaaat ctcacacaag gtgaaggaac ttacgcctga gtctattgtt gatgttattg gtgtaatcaa acctactgag cgacccgtga catccgtcag ttgctcgaac tatgaactgc atgcgacatc tatagaagtt gtgagcaagg cagctgtacc gcttcctttt ccgcttcacg accagaacgc gaagctggac acgcgcctga acaaccgata catcgacatg cgaacaccgt tggcggttgc tatgatacgt ctggtttcag ccgctgcgca gagcttccgc acgcagcttt tgtcccgcga ctttgtggaa cttcacacgc caaagatcat aggcacagca tcagagggtg gtagtgcgct cttctcactg gattactttg gtcgtcaagc cttcttggcg cagtcgcctc agttgtacaa gcaaatggca ataatgggtg atggtatgcg agtgttcgaa attgggcccg ttttccgcgc ggagaagagt cttacgcatc gtcatctcac ggagttcaca ggactggatg ctgaatttac agtcgacgaa ttctatactg agttgttgga tgtgctagag tcaatattat gcggcatgat ttggaaactt cagacagaaa acgccgagtg gatcagccag gcacgagcgg cattaatgga gttggacagt actgaggact gcggcactgt ggtgcctcgt cgtgaagata tagtatgcga ggtagcggag gagataattg ccatgtacaa tatacgtttt gatgacgcgg cgagccgaat agcaccttcc gtgccgactg aaccctcgga ggatcgctat catgcgcgta tcggtacaag cgggccaaag gtgctgcgta tgacattcag ggatgcactg caactcatcc gggataacga tcatcaacac gctggaaggc aacaagaggt cgtgggggaa agtgacgaag aggttgtggt cgacttcaac ctcaaccagg agcgggcact cggggagttg attcggttac gctacggcgt cgatttgtac gttgtggatc aattcccact ggctgcacga cccttttaca cgatgccaca cccgagtgaa gagggtgtag cctgtggctt cgatatgtat ttgcgtggag aggaaatttg tagcggcggg cagcggatac acgacgtggc gcagctcatt catagcatgg agcagcggcg aatggaagca gcacatatga gggactacgt ggactcattc cggtacggtg cgtggccaca cggtggcttc ggcctcggac ttgaacgtat cgtgcttttt ctcttgggtg cgcgtgacat caggcgtgtt tcactatttc cccgtgatcc gaggcggttg gcgccatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t