SSGCID: Target Page

TrbrA.00792.a

Protein kinase, putative; likely master regulator

CENTER ID:	TrbrA.00792.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00792.a.B1.GE40764	full length		1	384

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00792.a.B1.PS38349	full length		1	384

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.9.4910.
UniProt:	Q38F57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSASRMVPAD FELGSALGAG SFSKVVTASH VPTGRRYAIK VVSKQQICTA PSDEEKRRMA EVAIREARML RMCSHPNIVR FHASMQGPQD LMYVTELCDG GELLEAIKAW GTIPLEAARH VLAELFSAIW YLHHAPKHTQ PGVPDAPLES ITILHRDIKP ENIMLMSDRH VRLIDFGTAV VCRSVDERPE GEHSSKGRAK TFCGTAPYMS PELLRNNYTC TASDYWACGC VLYHMLVGKR PFDGSTEYFL IQSILEKEPE YPEDLDDDAK DLIHRLLSKS PDERPGREEV QNHPFFVSID FDGLATKEVA PFWVRNVEWE PNSLFSTCVV CRRAFFLWRR RRHCHNCGKL VCSSCSANHI TIPGCSSASP ERVCDSCFGK IRGD

NT Sequence

atgtcagcgt cccggatggt gccggccgat tttgagctcg gatcagctct tggtgcggga tccttcagta aggtggtgac agccagccac gtaccaaccg gacggcgcta tgcgatcaag gtggtttcaa agcaacagat ctgtaccgct ccctccgatg aagaaaagag gcgtatggca gaagtggcca taagagaggc ccgcatgctt cgtatgtgca gccaccccaa cattgtccgg ttccacgcct caatgcaagg tccgcaggac cttatgtacg taacggaact ttgtgacggg ggggagctac tcgaagctat caaagcgtgg ggaacaatac cccttgaggc tgcacgccac gtcctcgccg agcttttttc ggctatatgg tatttgcatc atgcaccgaa acacacacag cccggagttc ctgatgcccc ccttgagtca ataacaatat tacatagaga tataaaaccc gaaaatatta tgctgatgag cgataggcac gtcaggctca ttgacttcgg tacggcggtg gtgtgtcggt cggttgacga acggccggaa ggtgaacaca gtagcaaagg aagggcgaag acattttgtg gtacagcacc ttacatgtca cctgaattgt tacggaacaa ctacacttgc actgcttctg attactgggc ctgtggttgc gtgttatacc acatgctggt gggcaagcgt ccttttgatg gatcaacgga gtactttctt atacagagta tcctcgaaaa ggaacccgag tatcccgagg acttagacga cgacgcgaag gatttgatac acaggttgct gtcaaaatca ccggatgaac gacccggtcg tgaagaggtg caaaatcatc cattttttgt gagtattgat tttgatggtc tcgcaacgaa ggaagtggcc cctttttggg tgaggaacgt tgagtgggag cccaattccc tatttagcac ctgcgtagtc tgtagaaggg cgttcttcct ctggaggcgt cgccgccact gtcataattg tgggaagttg gtttgtagtt cctgctccgc gaatcatatt accatacctg gttgttccag cgcctcccct gagagggttt gtgattcttg ctttggcaag attagggggg attga

Details for TrbrA.00792.a.B1.GE40764

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS ASRMVPADFE LGSALGAGSF SKVVTASHVP TGRRYAIKVV SKQQICTAPS DEEKRRMAEV AIREARMLRM CSHPNIVRFH ASMQGPQDLM YVTELCDGGE LLEAIKAWGT IPLEAARHVL AELFSAIWYL HHAPKHTQPG VPDAPLESIT ILHRDIKPEN IMLMSDKHVR LIDFGTAVVC RSVDERPEGE HSSKGRAKTF CGTAPYMSPE LLRNNYTCTA SDYWACGCVL YHMLVGKRPF DGSTEYFLIQ SILEKEPEYP EDLDDDAKDL IHRLLSKSPD ERPGREEVQN HPFFVSIDFD GLATKEVTPF WVRNVEWEPN SLFSTCVVCR RAFFLWRRRR HCHNCGKLVC SSCSANHITI PGCSSASPER VCDS

Validated NT Sequence

caagaatcac aaaccctctc aggggaggcg ctggaacaac caggtatggt aatatgattc gcggagcagg aactacaaac caacttccca caattatgac agtggcggcg acgcctccag aggaagaacg cccttctaca gactacgcag gtgctaaata gggaattggg ctcccactca acgttcctca cccaaaaagg ggtcacttcc ttcgttgcga gaccatcaaa atcaatactc acaaaaaatg gatgattttg cacctcttca cgaccgggtc gttcatccgg tgattttgac agcaacctgt gtatcaaatc cttcgcgtcg tcgtctaagt cctcgggata ctcgggttcc ttttcgagga tactctgtat aagaaagtac tccgttgatc catcaaaagg acgcttgccc accagcatgt ggtataacac gcaaccacag gcccagtaat cagaagcagt gcaagtgtag ttgttccgta acaattcagg tgacatgtaa ggtgctgtac cacaaaatgt cttcgccctg cctttgctac tgtgttcacc ttccggccgt tcgtcaaccg agcgacacac caccgccgta ccgaagtcaa tgagcctgac gtgcttatcg ctcatcagca taatattttc gggctttata tctctatgta atattgttat tgactcaagg ggggcatcag gaactccggg ctgtgtgtgt ttcggtgcat gatgcaaata ccatatagcc gaaaaaagct cggcgaggac gtggcgtgca gcctcaaggg gtattgttcc ccacgctttg atagcttcga gtagctcccc cccgtcacaa agttccgtta cgtacataag gtcctgcgga ccttgcattg aggcgtggaa ccggacaatg ttggggtggc tgcacatacg aagcatgcgg gcctctctta tggccacttc tgccatacgc ctcttttctt catcggaggg agcggtacag atctgttgct ttgaaaccac cttgatcgca tagcgccgtc cggttggtac gtggctggct gtcaccacct tactgaagga tcccgcacca agagctgatc cgagctcaaa atcggccggc accatccggg acgctgacat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHMS ASRMVPADFE LGSALGAGSF SKVVTASHVP TGRRYAIKVV SKQQICTAPS DEEKRRMAEV AIREARMLRM CSHPNIVRFH ASMQGPQDLM YVTELCDGGE LLEAIKAWGT IPLEAARHVL AELFSAIWYL HHAPKHTQPG VPDAPLESIT ILHRDIKPEN IMLMSDRHVR LIDFGTAVVC RSVDERPEGE HSSKGRAKTF CGTAPYMSPE LLRNNYTCTA SDYWACGCVL YHMLVGKRPF DGSTEYFLIQ SILEKEPEYP EDLDDDAKDL IHRLLSKSPD ERPGREEVQN HPFFVSIDFD GLATKEVAPF WVRNVEWEPN SLFSTCVVCR RAFFLWRRRR HCHNCGKLVC SSCSANHITI PGCSSASPER VCDSCFGKIR GD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag cgtcccggat ggtgccggcc gattttgagc tcggatcagc tcttggtgcg ggatccttca gtaaggtggt gacagccagc cacgtaccaa ccggacggcg ctatgcgatc aaggtggttt caaagcaaca gatctgtacc gctccctccg atgaagaaaa gaggcgtatg gcagaagtgg ccataagaga ggcccgcatg cttcgtatgt gcagccaccc caacattgtc cggttccacg cctcaatgca aggtccgcag gaccttatgt acgtaacgga actttgtgac gggggggagc tactcgaagc tatcaaagcg tggggaacaa taccccttga ggctgcacgc cacgtcctcg ccgagctttt ttcggctata tggtatttgc atcatgcacc gaaacacaca cagcccggag ttcctgatgc cccccttgag tcaataacaa tattacatag agatataaaa cccgaaaata ttatgctgat gagcgatagg cacgtcaggc tcattgactt cggtacggcg gtggtgtgtc ggtcggttga cgaacggccg gaaggtgaac acagtagcaa aggaagggcg aagacatttt gtggtacagc accttacatg tcacctgaat tgttacggaa caactacact tgcactgctt ctgattactg ggcctgtggt tgcgtgttat accacatgct ggtgggcaag cgtccttttg atggatcaac ggagtacttt cttatacaga gtatcctcga aaaggaaccc gagtatcccg aggacttaga cgacgacgcg aaggatttga tacacaggtt gctgtcaaaa tcaccggatg aacgacccgg tcgtgaagag gtgcaaaatc atccattttt tgtgagtatt gattttgatg gtctcgcaac gaaggaagtg gccccttttt gggtgaggaa cgttgagtgg gagcccaatt ccctatttag cacctgcgta gtctgtagaa gggcgttctt cctctggagg cgtcgccgcc actgtcataa ttgtgggaag ttggtttgta gttcctgctc cgcgaatcat attaccatac ctggttgttc cagcgcctcc cctgagaggg tttgtgattc ttgctttggc aagattaggg gggattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for TrbrA.00792.a.B1.PS38349

PURIFICATION DATe:	9/12/2017
CONCENTRATION:	43.9mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMS ASRMVPADFE LGSALGAGSF SKVVTASHVP TGRRYAIKVV SKQQICTAPS DEEKRRMAEV AIREARMLRM CSHPNIVRFH ASMQGPQDLM YVTELCDGGE LLEAIKAWGT IPLEAARHVL AELFSAIWYL HHAPKHTQPG VPDAPLESIT ILHRDIKPEN IMLMSDRHVR LIDFGTAVVC RSVDERPEGE HSSKGRAKTF CGTAPYMSPE LLRNNYTCTA SDYWACGCVL YHMLVGKRPF DGSTEYFLIQ SILEKEPEYP EDLDDDAKDL IHRLLSKSPD ERPGREEVQN HPFFVSIDFD GLATKEVAPF WVRNVEWEPN SLFSTCVVCR RAFFLWRRRR HCHNCGKLVC SSCSANHITI PGCSSASPER VCDSCFGKIR GD

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAG CGTCCCGGAT GGTGCCGGCC GATTTTGAGC TCGGATCAGC TCTTGGTGCG GGATCCTTCA GTAAGGTGGT GACAGCCAGC CACGTACCAA CCGGACGGCG CTATGCGATC AAGGTGGTTT CAAAGCAACA GATCTGTACC GCTCCCTCCG ATGAAGAAAA GAGGCGTATG GCAGAAGTGG CCATAAGAGA GGCCCGCATG CTTCGTATGT GCAGCCACCC CAACATTGTC CGGTTCCACG CCTCAATGCA AGGTCCGCAG GACCTTATGT ACGTAACGGA ACTTTGTGAC GGGGGGGAGC TACTCGAAGC TATCAAAGCG TGGGGAACAA TACCCCTTGA GGCTGCACGC CACGTCCTCG CCGAGCTTTT TTCGGCTATA TGGTATTTGC ATCATGCACC GAAACACACA CAGCCCGGAG TTCCTGATGC CCCCCTTGAG TCAATAACAA TATTACATAG AGATATAAAA CCCGAAAATA TTATGCTGAT GAGCGATAGG CACGTCAGGC TCATTGACTT CGGTACGGCG GTGGTGTGTC GGTCGGTTGA CGAACGGCCG GAAGGTGAAC ACAGTAGCAA AGGAAGGGCG AAGACATTTT GTGGTACAGC ACCTTACATG TCACCTGAAT TGTTACGGAA CAACTACACT TGCACTGCTT CTGATTACTG GGCCTGTGGT TGCGTGTTAT ACCACATGCT GGTGGGCAAG CGTCCTTTTG ATGGATCAAC GGAGTACTTT CTTATACAGA GTATCCTCGA AAAGGAACCC GAGTATCCCG AGGACTTAGA CGACGACGCG AAGGATTTGA TACACAGGTT GCTGTCAAAA TCACCGGATG AACGACCCGG TCGTGAAGAG GTGCAAAATC ATCCATTTTT TGTGAGTATT GATTTTGATG GTCTCGCAAC GAAGGAAGTG GCCCCTTTTT GGGTGAGGAA CGTTGAGTGG GAGCCCAATT CCCTATTTAG CACCTGCGTA GTCTGTAGAA GGGCGTTCTT CCTCTGGAGG CGTCGCCGCC ACTGTCATAA TTGTGGGAAG TTGGTTTGTA GTTCCTGCTC CGCGAATCAT ATTACCATAC CTGGTTGTTC CAGCGCCTCC CCTGAGAGGG TTTGTGATTC TTGCTTTGGC AAGATTAGGG GGGATTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT