SSGCID: Target Page

TrbrA.00729.b

S-adenosylmethionine synthetase

CENTER ID:	TrbrA.00729.b
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00729.b.B1.GE40497	full length		1	397

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00729.b.B1.PW38371	full length		1	397

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.6.4870
RefSeq:	XP_845604.1
UniProt:	Q586F9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVRQIFFTS EHVSEGHPDK LCDQVSDAVL DACLEQDPLS KVACETCSKT GMVLVFGEIT TKAVIDYQKV VRDTVKEIGF DNTEKGLDYR SLNLLVAVEQ QSPDIYQGLG DFGGENLGAG DQGMMFGYAT NETETLMPLS YELARGLAMT YSELRRNGGL PWARPDAKTQ VTVQYEYDTR GGKQLLTPKR VAVILVSAQL DDGVSNETIR EDLVKKVIST TIPAKFLDEN TKYHLNPSGR FVIGGPHGDA GLTGRKIIVD TYGGWGAHGG GAFSGKDPSK VDRSAAYAAR WIAKSLVAAG LADRCLVQLS YAIGVAEPLN IHVDTYGTGK RDDEYILGIV QKNFRLRPYD IIKELDLRRP IYRQTACFGH FGRVDTNGKG GFTWEVPKTL EGLSDVQ

NT Sequence

atgtccgtgc gccagatatt cttcacgtcg gagcatgtga gtgaaggtca ccccgataaa ctatgcgatc aggtgtcgga tgccgtactg gatgcatgcc ttgagcagga cccgctctct aaggttgcat gcgagacgtg ctcaaagacg gggatggtgc ttgtgtttgg tgagatcaca acgaaggctg tgattgacta ccagaaggtc gtacgtgaca cggttaagga gatcggcttc gataacactg agaaggggtt ggactaccgt tcactgaacc tgcttgtggc cgttgagcaa caatcacccg atatttacca gggcctcggt gactttggcg gggagaacct cggtgctgga gaccagggca tgatgtttgg ttatgcgacg aatgagacgg agacactaat gccactctct tacgaacttg cgcgcggtct tgcaatgaca tacagcgagt tgcgacgcaa tggtggtctt ccgtgggcgc gccccgacgc taagacgcaa gtgacagtgc agtacgaata tgacacccgc ggtggcaagc agttgctgac acctaagcga gtggctgtga ttcttgtttc tgcccagctt gacgacggtg tgtcaaatga aaccatccgc gaggacctgg tgaagaaggt aatcagcacc accattcccg cgaagttcct cgatgagaac accaaatatc acttgaaccc atctgggcgt ttcgtcatcg gtggcccaca tggtgacgct gggttaactg gtcgcaagat aattgttgac acgtatggcg gttggggtgc ccatggtggt ggcgcgtttt ctggcaagga tccctcgaag gtggaccggt cggcagcgta tgctgctcgc tggattgcga agtctctggt ggccgcaggt cttgcagatc ggtgccttgt acaactttcg tacgccattg gagttgcgga accgctcaat atccatgtcg acacatatgg cactggtaag cgcgatgacg agtatatatt gggtattgtg cagaaaaact tccgactgcg cccgtacgac ataatcaagg aacttgatct tcgtcggccg atataccgcc agactgcctg tttcgggcac tttgggcgtg tggacacgaa cgggaaggga ggattcacgt gggaggtgcc caaaacacta gagggtctta gtgacgtgca gtag

Details for TrbrA.00729.b.B1.GE40497

HARVESTED ON:	8/10/2016
SEQUENCED ON:	8/11/2016
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS VRQIFFTSEH VSEGHPDKLC DQVSDAVLDA CLEQDPLSKV ACETCSKTGM VLVFGEITTK AVIDYQKVVR DTVKEIGFDN TEKGLDYRSL NLLVAVEQQS PDIYQGLGDF GGENLGAGDQ GMMFGYATNE TETLMPLSYE LARGLAMTYS ELRRNGGLPW ARPDAKTQVT VQYEYDTRGG KQLLTPKRVA VILVSAQLDD GVSNETIRED LVKKVISTTI PAKFLDENTK YHLNPSGRFV IGGPHGDAGL TGRKIIVDTY GGWGAHGGGA FSGKDPSKVD RSAAYAARWI AKSLVAAGLA DRCLVQLSYA IGVAEPLNIH VDTYGTGKRD DEYILGIVQK NFRLRPYDII KELDLRRPIY RQTACFGHFG RVDTNGKGGF TWEVPKTLEG LSDVQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg tgcgccagat attcttcacg tcggagcatg tgagtgaagg tcaccccgat aaactatgcg atcaggtgtc ggatgccgta ctggatgcat gccttgagca ggacccgctc tctaaggttg catgcgagac gtgctcaaag acggggatgg tgcttgtgtt tggtgagatc acaacgaagg ctgtgattga ctaccagaag gtcgtacgtg acacggttaa ggagatcggc ttcgataaca ctgagaaggg gttggactac cgttcactga acctgcttgt ggccgttgag caacaatcac ccgatattta ccagggcctc ggtgactttg gcggggagaa cctcggtgct ggagaccagg gcatgatgtt tggttatgcg acgaatgaga cggagacact aatgccactc tcttacgaac ttgcgcgcgg tcttgcaatg acatacagcg agttgcgacg caatggtggt cttccgtggg cgcgccccga cgctaagacg caagtgacag tgcagtacga atatgacacc cgcggtggca agcagttgct gacacctaag cgagtggctg tgattcttgt ttctgcccag cttgacgacg gtgtgtcaaa tgaaaccatc cgcgaggacc tggtgaagaa ggtaatcagc accaccattc ccgcgaagtt cctcgatgag aacaccaaat atcacttgaa cccatctggg cgtttcgtca tcggtggccc acatggtgac gctgggttaa ctggtcgcaa gataattgtt gacacgtatg gcggttgggg tgcccatggt ggtggcgcgt tttctggcaa ggatccctcg aaggtggacc ggtcggcagc gtatgctgct cgctggattg cgaagtctct ggtggccgca ggtcttgcag atcggtgcct tgtacaactt tcgtacgcca ttggagttgc ggaaccgctc aatatccatg tcgacacata tggcactggt aagcgcgatg acgagtatat attgggtatt gtgcagaaaa acttccgact gcgcccgtac gacataatca aggaacttga tcttcgtcgg ccgatatacc gccagactgc ctgtttcggg cactttgggc gtgtggacac gaacgggaag ggaggattca cgtgggaggt gcccaaaaca ctagagggtc ttagtgacgt gcagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for TrbrA.00729.b.B1.PW38371

PURIFICATION DATe:	10/16/2017
CONCENTRATION:	36.2mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

Added 5mM Methionine and ATP to lysis buffer Combined two pellets for purification

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG TGCGCCAGAT ATTCTTCACG TCGGAGCATG TGAGTGAAGG TCACCCCGAT AAACTATGCG ATCAGGTGTC GGATGCCGTA CTGGATGCAT GCCTTGAGCA GGACCCGCTC TCTAAGGTTG CATGCGAGAC GTGCTCAAAG ACGGGGATGG TGCTTGTGTT TGGTGAGATC ACAACGAAGG CTGTGATTGA CTACCAGAAG GTCGTACGTG ACACGGTTAA GGAGATCGGC TTCGATAACA CTGAGAAGGG GTTGGACTAC CGTTCACTGA ACCTGCTTGT GGCCGTTGAG CAACAATCAC CCGATATTTA CCAGGGCCTC GGTGACTTTG GCGGGGAGAA CCTCGGTGCT GGAGACCAGG GCATGATGTT TGGTTATGCG ACGAATGAGA CGGAGACACT AATGCCACTC TCTTACGAAC TTGCGCGCGG TCTTGCAATG ACATACAGCG AGTTGCGACG CAATGGTGGT CTTCCGTGGG CGCGCCCCGA CGCTAAGACG CAAGTGACAG TGCAGTACGA ATATGACACC CGCGGTGGCA AGCAGTTGCT GACACCTAAG CGAGTGGCTG TGATTCTTGT TTCTGCCCAG CTTGACGACG GTGTGTCAAA TGAAACCATC CGCGAGGACC TGGTGAAGAA GGTAATCAGC ACCACCATTC CCGCGAAGTT CCTCGATGAG AACACCAAAT ATCACTTGAA CCCATCTGGG CGTTTCGTCA TCGGTGGCCC ACATGGTGAC GCTGGGTTAA CTGGTCGCAA GATAATTGTT GACACGTATG GCGGTTGGGG TGCCCATGGT GGTGGCGCGT TTTCTGGCAA GGATCCCTCG AAGGTGGACC GGTCGGCAGC GTATGCTGCT CGCTGGATTG CGAAGTCTCT GGTGGCCGCA GGTCTTGCAG ATCGGTGCCT TGTACAACTT TCGTACGCCA TTGGAGTTGC GGAACCGCTC AATATCCATG TCGACACATA TGGCACTGGT AAGCGCGATG ACGAGTATAT ATTGGGTATT GTGCAGAAAA ACTTCCGACT GCGCCCGTAC GACATAATCA AGGAACTTGA TCTTCGTCGG CCGATATACC GCCAGACTGC CTGTTTCGGG CACTTTGGGC GTGTGGACAC GAACGGGAAG GGAGGATTCA CGTGGGAGGT GCCCAAAACA CTAGAGGGTC TTAGTGACGT GCAGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT