SSGCID: Target Page

TrbrA.00612.a

lysyl-tRNA synthetase, putative

CENTER ID:	TrbrA.00612.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00612.a.B1.GE42774	full length		1	584

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00612.a.B1.PW38585	full length		1	584

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.8.1600
UniProt:	Q580G3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAVEELRAQ IDALASQISA VKKEQGASSE ACKSLVAKMT ELRKQLPQEK KEKKAEKAPE ISYYDTRLAM VKEMGPLGAA YPHKFHRDYT LPAFRECFKP MLQEKGQRLD KVVTIAGRIV VKRSSSSKLH FLALQGDGEV LQVISMASDY VNDGFADIHS KIKRGDIIGV RGVPSFSNTG EFSMSAYEIT LLSTCFHMLP DEHYGLSSVE QRFRQRYLDL IVNRENAKTF ILRSKIISYI RSFFDQKDFL EVETPMLNQI AGGAAARPFI THHNELNQTM YLRIAPELYL KKLVVGGLDR VYEIGKQFRN EGIDLTHNPE FTSVESYWAY ADYNDWMETT EELLYGMVMH LYGSPIVKYS PRDSEGRQLP EVTFNFNRPF KRLHIVPKLE EILGLKFPAD FYSSEANSFF LDICKKNQVE CNPPFTTTRL LDALVSHYLE PQCHDPTFLC DHPRIMSPLA KWHRKDLRLS ERFELFINKK EICNSYTELN SPLVQREEFE RQLRDREKGD DEAMDIDEGY VQALEYALPP TGGWGLGIDR LVMYLTSQNN IKEVLLFPAM KPEGTSSATF PPGTMLNGQG VPLL

NT Sequence

atgtcggctg tggaggagct ccgagctcaa atcgatgcgc tcgcgtcgca gatctctgcg gttaaaaagg aacagggcgc ctctagtgag gcgtgcaaat cccttgtggc taaaatgact gaacttcgaa agcaacttcc tcaagaaaag aaggaaaaga aagcggagaa ggccccggag atcagttact acgatacacg tctcgcaatg gtgaaagaaa tgggccctct cggtgccgcc taccctcata agttccaccg agactacacc ttacccgcct tccgagagtg tttcaagcca atgttgcagg aaaagggtca acgcctcgat aaggtcgtga cgattgccgg acgtatcgtc gtgaagagaa gctcgagctc caaactgcat tttttggctc ttcagggtga tggggaggta ctgcaggtta tatccatggc gagtgactac gttaatgatg gttttgcgga cattcacagc aagatcaagc gtggcgatat cattggtgtg cgaggtgtgc catcgttttc aaacacgggg gagttctcca tgagtgccta cgaaatcacg ctgctctcca catgttttca catgttgcca gatgagcatt acgggctgag ctcagtcgaa cagcgcttcc gtcagcgata cctcgacctg attgtgaatc gagaaaacgc gaagactttt atcctccgct ccaagatcat aagctacatc cgcagtttct ttgaccagaa ggacttccta gaggtggaaa cgcccatgtt gaaccagatc gccggtggtg ctgctgcgcg gccttttatc acacaccaca atgagctcaa ccagacaatg tacctgcgaa ttgcaccgga gttgtaccta aaaaaacttg ttgttggtgg cttggatcgt gtatatgaga tcggcaaaca gttccgcaac gaaggtatcg atctcacgca taatccggag ttcacgagtg tggagagtta ttgggcgtac gcagactaca atgattggat ggagaccact gaggagttac tttacggtat ggtcatgcat ctttacggat ctcctattgt gaagtactcc ccgagggatt cagagggccg tcagttacct gaggtaactt ttaacttcaa caggcccttc aaacgcctgc acattgttcc gaagttggag gagatactag gacttaaatt ccctgctgat ttctattcct ctgaggcaaa cagtttcttt ctggacattt gcaagaagaa ccaggttgag tgcaatcccc ccttcacaac gacacgccta ctagatgcgc ttgtctccca ctatcttgag ccacagtgcc acgacccgac atttctgtgc gaccacccgc gtataatgtc tccgttggca aagtggcacc gtaaagacct tcggctcagc gagcgcttcg agctgtttat taacaaaaaa gagatctgca attcatatac ggagttaaac agtcccctcg tgcagcgaga ggagtttgaa aggcaactgc gtgaccgcga gaagggtgat gacgaggcaa tggatattga tgagggttat gtacaagcat tggaatacgc gttgcctccc actggtggtt ggggtttggg tattgatcga ctcgtcatgt atctcacaag ccaaaacaac atcaaggaag tccttctatt tccagctatg aaacctgaag ggacatcctc tgccacattc ccaccaggaa caatgttgaa tggacaaggg gtgccacttc tttga

Details for TrbrA.00612.a.B1.GE42774

HARVESTED ON:	11/5/2018
SEQUENCED ON:	11/7/2018
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS AVEELRAQID ALASQISAVK KEQGASSEAC KSLVAKMTEL RKQLPQEKKE KKAEKAPEIS YYDTRLAMVK EMGPLGAAYP HKFHRDYTLP AFRECFKPML QEKGQRLDKV VTIAGRIVVK RSSSSKLHFL ALQGDGEVLQ VISMASDYVN DGFADIHSKI KRGDIIGVRG VPSFSNTGEF SMSAYEITLL STCFHMLPDE HYGLSSVEQR FRQRYLDLIV NRENAKTFIL RSKIISYIRS FFDQKDFLEV ETPMLNQIAG GAAARPFITH HNELNQTMYL RIAPELYLKK LVVGGLDRVY EIGKQFRNEG IDLTHNPEFT SVESYWAYAD YNDWMETTEE LLYGMVMHLY GSPIVKYSPR DSEGRQLPEV TFNFNRPFKR LHIVPKLEEI LGLKFPADFY SSEANSFFLD ICKKNQVECN PPFTTTRLLD ALVSHYLEPQ CHDPTFLCDH PRIMSPLAKW HRKDLRLSER FELFINKKEI CNSYTELNSP LVQREEFERQ LRDREKGDDE AMDIDEGYVQ ALEYALPPTG GWGLGIDRLV MYLTSQNNIK EVLLFPAMKP EGTSSATFPP GTMLNGQGVP LL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgg ctgtggagga gctccgagct caaatcgatg cgctcgcgtc gcagatctct gcggttaaaa aggaacaggg cgcctctagt gaggcgtgca aatcccttgt ggctaaaatg actgaacttc gaaagcaact tcctcaagaa aagaaggaaa agaaagcgga gaaggccccg gagatcagtt actacgatac acgtctcgca atggtgaaag aaatgggccc tctcggtgcc gcctaccctc ataagttcca ccgagactac accttacccg ccttccgaga gtgtttcaag ccaatgttgc aggaaaaggg tcaacgcctc gataaggtcg tgacgattgc cggacgtatc gtcgtgaaga gaagctcgag ctccaaactg cattttttgg ctcttcaggg tgatggggag gtactgcagg ttatatccat ggcgagtgac tacgttaatg atggttttgc ggacattcac agcaagatca agcgtggcga tatcattggt gtgcgaggtg tgccatcgtt ttcaaacacg ggggagttct ccatgagtgc ctacgaaatc acgctgctct ccacatgttt tcacatgttg ccagatgagc attacgggct gagctcagtc gaacagcgct tccgtcagcg atacctcgac ctgattgtga atcgagaaaa cgcgaagact tttatcctcc gctccaagat cataagctac atccgcagtt tctttgacca gaaggacttc ctagaggtgg aaacgcccat gttgaaccag atcgccggtg gtgctgctgc gcggcctttt atcacacacc acaatgagct caaccagaca atgtacctgc gaattgcacc ggagttgtac ctaaaaaaac ttgttgttgg tggcttggat cgtgtatatg agatcggcaa acagttccgc aacgaaggta tcgatctcac gcataatccg gagttcacga gtgtggagag ttattgggcg tacgcagact acaatgattg gatggagacc actgaggagt tactttacgg tatggtcatg catctttacg gatctcctat tgtgaagtac tccccgaggg attcagaggg ccgtcagtta cctgaggtaa cttttaactt caacaggccc ttcaaacgcc tgcacattgt tccgaagttg gaggagatac taggacttaa attccctgct gatttctatt cctctgaggc aaacagtttc tttctggaca tttgcaagaa gaaccaggtt gagtgcaatc cccccttcac aacgacacgc ctactagatg cgcttgtctc ccactatctt gagccacagt gccacgaccc gacatttctg tgcgaccacc cgcgtataat gtctccgttg gcaaagtggc accgtaaaga ccttcggctc agcgagcgct tcgagctgtt tattaacaaa aaagagatct gcaattcata tacggagtta aacagtcccc tcgtgcagcg agaggagttt gaaaggcaac tgcgtgaccg cgagaagggt gatgacgagg caatggatat tgatgagggt tatgtacaag cattggaata cgcgttgcct cccactggtg gttggggttt gggtattgat cgactcgtca tgtatctcac aagccaaaac aacatcaagg aagtccttct atttccagct atgaaacctg aagggacatc ctctgccaca ttcccaccag gaacaatgtt gaatggacaa ggggtgccac ttctttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for TrbrA.00612.a.B1.PW38585

PURIFICATION DATe:	12/31/2018
CONCENTRATION:	19.04mg/ml
OBSERVED MW:	68kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCGG CTGTGGAGGA GCTCCGAGCT CAAATCGATG CGCTCGCGTC GCAGATCTCT GCGGTTAAAA AGGAACAGGG CGCCTCTAGT GAGGCGTGCA AATCCCTTGT GGCTAAAATG ACTGAACTTC GAAAGCAACT TCCTCAAGAA AAGAAGGAAA AGAAAGCGGA GAAGGCCCCG GAGATCAGTT ACTACGATAC ACGTCTCGCA ATGGTGAAAG AAATGGGCCC TCTCGGTGCC GCCTACCCTC ATAAGTTCCA CCGAGACTAC ACCTTACCCG CCTTCCGAGA GTGTTTCAAG CCAATGTTGC AGGAAAAGGG TCAACGCCTC GATAAGGTCG TGACGATTGC CGGACGTATC GTCGTGAAGA GAAGCTCGAG CTCCAAACTG CATTTTTTGG CTCTTCAGGG TGATGGGGAG GTACTGCAGG TTATATCCAT GGCGAGTGAC TACGTTAATG ATGGTTTTGC GGACATTCAC AGCAAGATCA AGCGTGGCGA TATCATTGGT GTGCGAGGTG TGCCATCGTT TTCAAACACG GGGGAGTTCT CCATGAGTGC CTACGAAATC ACGCTGCTCT CCACATGTTT TCACATGTTG CCAGATGAGC ATTACGGGCT GAGCTCAGTC GAACAGCGCT TCCGTCAGCG ATACCTCGAC CTGATTGTGA ATCGAGAAAA CGCGAAGACT TTTATCCTCC GCTCCAAGAT CATAAGCTAC ATCCGCAGTT TCTTTGACCA GAAGGACTTC CTAGAGGTGG AAACGCCCAT GTTGAACCAG ATCGCCGGTG GTGCTGCTGC GCGGCCTTTT ATCACACACC ACAATGAGCT CAACCAGACA ATGTACCTGC GAATTGCACC GGAGTTGTAC CTAAAAAAAC TTGTTGTTGG TGGCTTGGAT CGTGTATATG AGATCGGCAA ACAGTTCCGC AACGAAGGTA TCGATCTCAC GCATAATCCG GAGTTCACGA GTGTGGAGAG TTATTGGGCG TACGCAGACT ACAATGATTG GATGGAGACC ACTGAGGAGT TACTTTACGG TATGGTCATG CATCTTTACG GATCTCCTAT TGTGAAGTAC TCCCCGAGGG ATTCAGAGGG CCGTCAGTTA CCTGAGGTAA CTTTTAACTT CAACAGGCCC TTCAAACGCC TGCACATTGT TCCGAAGTTG GAGGAGATAC TAGGACTTAA ATTCCCTGCT GATTTCTATT CCTCTGAGGC AAACAGTTTC TTTCTGGACA TTTGCAAGAA GAACCAGGTT GAGTGCAATC CCCCCTTCAC AACGACACGC CTACTAGATG CGCTTGTCTC CCACTATCTT GAGCCACAGT GCCACGACCC GACATTTCTG TGCGACCACC CGCGTATAAT GTCTCCGTTG GCAAAGTGGC ACCGTAAAGA CCTTCGGCTC AGCGAGCGCT TCGAGCTGTT TATTAACAAA AAAGAGATCT GCAATTCATA TACGGAGTTA AACAGTCCCC TCGTGCAGCG AGAGGAGTTT GAAAGGCAAC TGCGTGACCG CGAGAAGGGT GATGACGAGG CAATGGATAT TGATGAGGGT TATGTACAAG CATTGGAATA CGCGTTGCCT CCCACTGGTG GTTGGGGTTT GGGTATTGAT CGACTCGTCA TGTATCTCAC AAGCCAAAAC AACATCAAGG AAGTCCTTCT ATTTCCAGCT ATGAAACCTG AAGGGACATC CTCTGCCACA TTCCCACCAG GAACAATGTT GAATGGACAA GGGGTGCCAC TTCTTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT