SSGCID: Target Page

TrbrA.00600.a

Tryptophanyl-tRNA synthetase 2

CENTER ID:	TrbrA.00600.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00600.a.B1.GE42773	full length		1	473

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.8.2240
UniProt:	Q581V8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRASHVCTS CELLGFQKQF LMLSNRGNSS GDNDVIDTSG GKFRNGNGDG KDDDVVTPWN VSATGVRGVN YDRVLVRFKS QPIGEPLLKR MQDVCVERVK CNNSERGHNA ERSAVGEEVQ PLHHFFRRGI AFSHRDFDIA LTNVQEALRS GTQGAYLYTG RGPSTRTMHL GHVIPFMLTR YLQDALGLPL VIQITDDEKF FFRDVPLHDN RCSDGIDIVT ENIKDIIAFG FDPKRTFIFR NTSYMGSMYP TVVRLQRTMT LSAVKNTLGL IDNDNIGKAS FAATQAAPCF SSSFPCILGE QDFEKPLQCI VPCAIDQDPY FVLARASASR MKYRAPALLH TKFLPALKGM RLKMSSSAEE SGVITLHDSP EQVQKKMKKA FSGGSGTLDD MKTKGVDLEA DVAYQFIRFF SPDDEMVGEV SAKYVVGEMN SCYVKSLAAD VVVRHVLRNW QAKRKLVTDE EVRRFTEVRN IMA

NT Sequence

atgcggcggg cttcgcatgt ttgcacgtcg tgtgaactgc tgggtttcca gaagcaattt ctcatgctct ccaaccgcgg taatagcagt ggtgataatg acgtcatcga tacaagtggc ggcaagttcc gcaacggcaa tggggatgga aaagacgacg atgttgtgac accatggaat gtttctgcca ccggagtgcg gggtgtcaac tatgatcgtg tgttggtgcg atttaagtcc caacctatcg gtgagccact gttaaaaagg atgcaggatg tttgtgttga aagagtgaaa tgcaataata gcgaaagagg acacaatgcg gaaaggagtg cagtgggtga agaggtgcag ccattgcatc acttcttcag gcgtggcata gccttttcgc acagagattt tgacatcgca ctgaccaacg tgcaggaagc cttaagatcg ggaactcagg gagcgtattt atacactggt cgcggtccga gtacgcggac aatgcatctt ggacacgtga taccctttat gcttacgcgc tacctgcagg atgcgctcgg gctgccgcta gtcatccaaa tcaccgacga tgagaagttt ttttttcgcg atgtaccgtt gcatgacaac cgatgctcgg acggaattga tattgtaacc gaaaacataa aggatattat tgcatttggc tttgacccta aacggacgtt tattttccgg aacacctcct acatgggtag catgtatcct accgttgttc gcctgcagcg cacaatgaca ctcagtgccg tgaagaatac gctgggattg atcgataatg acaatatcgg gaaagcctcc ttcgctgcta ctcaagcagc gccttgcttc agtagctcct ttccgtgcat ccttggagaa caagattttg aaaagcctct acagtgtatt gtgccatgtg ccattgatca ggatccgtac ttcgttctgg cgcgggcatc ggcatcccgc atgaagtatc gagctcccgc actactgcat acaaaattcc ttccagcttt gaaggggatg agactaaaga tgagtagtag tgccgaggag agcggtgtga taacattgca cgactcaccg gagcaagtac aaaagaagat gaagaaggct ttctccggcg gtagtgggac actggacgac atgaagacga aaggcgtgga tttggaggcc gatgtggcct accaattcat tcggtttttc tctccggacg acgagatggt tggggaggta tcggcaaagt atgtggttgg tgagatgaac agctgttatg tgaagagctt agccgcagat gttgtcgtcc ggcatgtgct gcgcaactgg caagcgaagc gaaagctggt gacagatgag gaggttcggc ggtttacgga ggtgcgcaac attatggcgt ag

Details for TrbrA.00600.a.B1.GE42773

HARVESTED ON:	11/5/2018
SEQUENCED ON:	11/7/2018
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	50

Validated AA Sequence

Validated NT Sequence

aaaccgccga acctcctcat ctgtcaccag ctttcgcttc gcttgccagt tgcgcagcac atgccggacg acaacatctg cggctaagct cttcacataa cagctgttca tctcaccaac cacatacttt gccgatacct ccccaaccat ctcgtcgtcc ggagagaaaa accgaatgaa ttggtaggcc acatcggcct ccaaatccac gcctttcgtc ttcatgtcgt ccagtgtccc actaccgccg gagaaagcct tcttcatctt cttttgtact tgctccggtg agtcgtgcaa tgttatcaca ccgctctcct cggcactact actcatcttt agtctcatcc ccttcaaagc tggaaggaat tttgtatgca gtagtgcggg agctcgatac ttcatgcggg atgccgatgc ccgcgccaga acgaagtacg gatcctgatc aatggcacat ggcacaatac actgtagagg cttttcaaaa tcttgttctc caaggacgca cggaaaggag ctactgaagc aaggcgctgc ttgagtagca gcgaaggagg ctttcccgat attgtcatta tcgatcaatc ccagcgtatt cttcacggca ctgagtgtca ttgtgcgctg caggcgaaca acggtaggat acatgctacc catgtaggag gtgttccgga aaataaacgt ccgtttaggn nnaangccaa aatgcaataa tatcctttat gttttcggtt acaatatcaa ttccgtccga gcatcggttg tcatgcaacg gtacatcgcg aaaaaaaaac ttctcatcgt cggtgatttg gatgactagc ggcagcccga gcgcatcctg caggtagcgc gtaagcataa agggtatcac gtgtccaaga tgcattgtcc gcgtactcgg accacgacca gtgtataaat acgctccctg agttcccgat cttaaggctt cctgcacgtt ggtcagtgcg atgtcaaaat ctctgtgcga aaaggctatg ccacgcctga agaagtgatg caatggctgc acctcttcac ccactgcact cctttccgca ttgtgtcctc tttcgctatt attgcatttc actctttcaa cacaaacatc ctgcatcctt tttaacagtg gctcaccgat aggttgggac ttaaatcgca ccaacacacg atcatagttg acaccccgca ctccggtggc agaaacattc catggtgtca caacatcgtc gtcttttcca tccccattgc cgttgcggaa cttgccgcca cttgtatcga tgacgtcatt atcaccactg ctattaccgc ggttggagag catgagaaat tgcttctgga aacccagcag ttcacacgac gtgcaaacat gcgaagcccg ccgcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHMR RASHVCTSCE LLGFQKQFLM LSNRGNSSGD NDVIDTSGGK FRNGNGDGKD DDVVTPWNVS ATGVRGVNYD RVLVRFKSQP IGEPLLKRMQ DVCVERVKCN NSERGHNAER SAVGEEVQPL HHFFRRGIAF SHRDFDIALT NVQEALRSGT QGAYLYTGRG PSTRTMHLGH VIPFMLTRYL QDALGLPLVI QITDDEKFFF RDVPLHDNRC SDGIDIVTEN IKDIIAFGFD PKRTFIFRNT SYMGSMYPTV VRLQRTMTLS AVKNTLGLID NDNIGKASFA ATQAAPCFSS SFPCILGEQD FEKPLQCIVP CAIDQDPYFV LARASASRMK YRAPALLHTK FLPALKGMRL KMSSSAEESG VITLHDSPEQ VQKKMKKAFS GGSGTLDDMK TKGVDLEADV AYQFIRFFSP DDEMVGEVSA KYVVGEMNSC YVKSLAADVV VRHVLRNWQA KRKLVTDEEV RRFTEVRNIM A

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcggc gggcttcgca tgtttgcacg tcgtgtgaac tgctgggttt ccagaagcaa tttctcatgc tctccaaccg cggtaatagc agtggtgata atgacgtcat cgatacaagt ggcggcaagt tccgcaacgg caatggggat ggaaaagacg acgatgttgt gacaccatgg aatgtttctg ccaccggagt gcggggtgtc aactatgatc gtgtgttggt gcgatttaag tcccaaccta tcggtgagcc actgttaaaa aggatgcagg atgtttgtgt tgaaagagtg aaatgcaata atagcgaaag aggacacaat gcggaaagga gtgcagtggg tgaagaggtg cagccattgc atcacttctt caggcgtggc atagcctttt cgcacagaga ttttgacatc gcactgacca acgtgcagga agccttaaga tcgggaactc agggagcgta tttatacact ggtcgcggtc cgagtacgcg gacaatgcat cttggacacg tgataccctt tatgcttacg cgctacctgc aggatgcgct cgggctgccg ctagtcatcc aaatcaccga cgatgagaag tttttttttc gcgatgtacc gttgcatgac aaccgatgct cggacggaat tgatattgta accgaaaaca taaaggatat tattgcattt ggctttgacc ctaaacggac gtttattttc cggaacacct cctacatggg tagcatgtat cctaccgttg ttcgcctgca gcgcacaatg acactcagtg ccgtgaagaa tacgctggga ttgatcgata atgacaatat cgggaaagcc tccttcgctg ctactcaagc agcgccttgc ttcagtagct cctttccgtg catccttgga gaacaagatt ttgaaaagcc tctacagtgt attgtgccat gtgccattga tcaggatccg tacttcgttc tggcgcgggc atcggcatcc cgcatgaagt atcgagctcc cgcactactg catacaaaat tccttccagc tttgaagggg atgagactaa agatgagtag tagtgccgag gagagcggtg tgataacatt gcacgactca ccggagcaag tacaaaagaa gatgaagaag gctttctccg gcggtagtgg gacactggac gacatgaaga cgaaaggcgt ggatttggag gccgatgtgg cctaccaatt cattcggttt ttctctccgg acgacgagat ggttggggag gtatcggcaa agtatgtggt tggtgagatg aacagctgtt atgtgaagag cttagccgca gatgttgtcg tccggcatgt gctgcgcaac tggcaagcga agcgaaagct ggtgacagat gaggaggttc ggcggtttac ggaggtgcgc aacattatgg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat