SSGCID: Target Page

TrbrA.00436.a

protein kinase A catalytic subunit

CENTER ID:	TrbrA.00436.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.00436.a.B1.GE35078	full length		1	337

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.10.13010

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKSDGCLTRK DVSSWKLQDL EIRETIGTGT FGRVRLCRHR ASGQYVVLKI LKKQEILRMK QVDHILAESS ILQELSHPFI VTMYKGFMDD DRLYLLLEYV VGGELFSHLR KAGKFPNDVA KFYSAEVVLA FEYIHECGIV YRDLKPENVL LDKQGNIKIT DFGFAKRVRD RTYTLCGTPE YLAPEIIQSK GHDRAVDWWT LGILLYEMLV GYPPFFDESP FRTYEKILEG KLQFPKWVEM RAKDLIKSFL TIEPTKRLGS VKRGVQDVKR HKFYSGVNWN VLLQRGVAAP IHVRLNNDGD TRYFDRYPES PRHPLQPLTE AQQELFSGFC DGEYTRI

NT Sequence

atgaagtcgg atgggtgcct gacaaggaag gacgtgtcca gctggaagct gcaagacctg gagatccgag agacaatagg taccggtact tttgggcgcg tccgcctatg tagacacagg gcttctggac aatacgttgt tttgaagatt cttaaaaagc aggaaatttt gcgtatgaaa caggtagacc atatactggc cgagtcgagt attctacagg agttgagcca tccatttatt gtgacaatgt ataaaggttt tatggatgat gaccgattgt acctcctcct cgagtatgtg gtgggtggcg agctgttctc ccacctccgg aaggcgggaa aattccctaa tgatgtagcc aagttctact ccgcagaagt ggttttggcg tttgaatata ttcatgagtg cggcatcgta taccgtgact tgaagccaga aaatgtgctt ttggacaagc agggaaacat taagattacg gactttgggt tcgcgaaacg cgttagggac agaacgtaca cgctatgtgg gactccagag tatcttgcgc cggagataat ccaaagtaaa ggtcacgatc gggctgtgga ttggtggaca ctcggaattc ttctctatga gatgcttgtc ggttatcctc cttttttcga cgagagtcct tttagaacat acgaaaaaat tttagagggg aaacttcagt ttccaaagtg ggtggagatg cgggcgaagg acctcataaa gagtttttta acaattgaac caacgaaacg ccttgggtca gtaaaacgtg gtgtgcagga cgttaagcga cacaagttct acagtggggt taattggaac gttctcctac agagaggtgt cgccgctccc attcacgtgc gtctaaacaa cgatggtgac actaggtact tcgatcgtta tccagaaagt cctcgccatc cacttcaacc tttgactgag gcacagcagg agttattctc ggggttttgc gatggtgaat acacgaggat ctga

Details for TrbrA.00436.a.B1.GE35078

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	102

Validated AA Sequence

MAHHHHHHMK SDGCLTRKDV SSWKLQDLEI RETIGTGTFG RVRLCRHRAS GQYVVLKILK KQEILRMKQV DHILAESSIL QELSHPFIVT MYKGFMDDDR LYLLLEYVVG GELFSHLRKA GKFPNDVAKF YSAEVVLAFE YIHECGIVYR DLKPENVLLD KQGNIKITDF GFAKRVRDRT YTLCGTPEYL APEIIQSKGH DRAVDWWTLG ILLYEMLVGY PPFFDESPFR TYEKILEGKL QFPKWVEMRA KDLIKSFLTI EPTKRLGSVK RGVQDVKRHK FYSGVNWNVL LQRGVAAPIH VRLNNDGDTX YFDRYPESPR HXLQPLTXAQ XXXXRGFAMV IXXDXXSXXX XXXX

Validated NT Sequence

ttgnttaact ttaagaagga gatataccat ggctcaccac caccaccacc atatgaagtc ggatgggtgc ttgacaagga aggacgtgtc cagctggaag ctgcaagacc tggagatccg agagacaata ggtaccggta cttttgggcg cgtccgccta tgtagacaca gggcttctgg acaatacgtt gttttgaaga ttcttaaaaa gcaggaaatt ttgcgtatga aacaggtaga ccatatactg gccgagtcga gtattctaca ggagttgagc catccattta ttgtgacaat gtataaaggt tttatggatg atgaccgatt gtacctcctc ctcgagtatg tggtgggtgg cgagctgttc tcccacctcc ggaaggcggg aaaattccct aatgatgtag ccaagttcta ctccgcagaa gtggttttgg cgtttgaata tattcatgag tgcggcatcg tataccgtga cttgaagcca gaaaatgtgc ttttggacaa gcagggaaac attaagatta cggactttgg gttcgcgaaa cgcgttaggg acagaacgta cacgctatgt gggactccag agtatcttgc gccggagata atccaaagta aaggtcacga tcgggctgtg gattggtgga cactcggaat tcttctctat gagatgcttg tcggttatcc tccttttttc gacgagagtc cttttagaac atacgaaaaa attttagagg ggaaacttca gtttccaaag tgggtggaga tgcgggcgaa ggacctcata aagagttttc taacaattga accaacgaaa cgccttgggt cagtaaaacg tggtgtgcag gacgttaagc gacacaagtt ctacagtggg gttaattgga acgttctcct acagagaggt gtcgccgctc ccattcacgt gcgtctaaac aacgatggtg acactagnta cttcgatcgt tatccagaaa gtcctcgcca tcnacttcaa cctttgactg angcacagcn gnagtnatnn cggggttttg cgatggtnat acncnangat cnanngagta nnnnnnncnn nnnnnnnnnc ccc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagt cggatgggtg cctgacaagg aaggacgtgt ccagctggaa gctgcaagac ctggagatcc gagagacaat aggtaccggt acttttgggc gcgtccgcct atgtagacac agggcttctg gacaatacgt tgttttgaag attcttaaaa agcaggaaat tttgcgtatg aaacaggtag accatatact ggccgagtcg agtattctac aggagttgag ccatccattt attgtgacaa tgtataaagg ttttatggat gatgaccgat tgtacctcct cctcgagtat gtggtgggtg gcgagctgtt ctcccacctc cggaaggcgg gaaaattccc taatgatgta gccaagttct actccgcaga agtggttttg gcgtttgaat atattcatga gtgcggcatc gtataccgtg acttgaagcc agaaaatgtg cttttggaca agcagggaaa cattaagatt acggactttg ggttcgcgaa acgcgttagg gacagaacgt acacgctatg tgggactcca gagtatcttg cgccggagat aatccaaagt aaaggtcacg atcgggctgt ggattggtgg acactcggaa ttcttctcta tgagatgctt gtcggttatc ctcctttttt cgacgagagt ccttttagaa catacgaaaa aattttagag gggaaacttc agtttccaaa gtgggtggag atgcgggcga aggacctcat aaagagtttt ttaacaattg aaccaacgaa acgccttggg tcagtaaaac gtggtgtgca ggacgttaag cgacacaagt tctacagtgg ggttaattgg aacgttctcc tacagagagg tgtcgccgct cccattcacg tgcgtctaaa caacgatggt gacactaggt acttcgatcg ttatccagaa agtcctcgcc atccacttca acctttgact gaggcacagc aggagttatt ctcggggttt tgcgatggtg aatacacgag gatctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat